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Entry version 154 (29 Sep 2021)
Sequence version 1 (01 Apr 1990)
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Protein

Negative regulator of SacY activity

Gene

sacX

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Negatively regulates SacY activity by catalyzing its phosphorylation on 'His-99'.

1 Publication

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system may be involved in sucrose transport.

1 Publication

Negatively regulates SacY.

1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei25Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU38410-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
4.A.1.2.10, the pts glucose-glucoside (glc) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Negative regulator of SacY activity
Alternative name(s):
PTS system sac EIIBC component
Including the following 2 domains:
Phosphotransferase enzyme IIB component (EC:2.7.1.-)
Alternative name(s):
PTS system EIIB component
Permease IIC component
Alternative name(s):
PTS system EIIC component
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:sacX
Synonyms:sacS
Ordered Locus Names:BSU38410
ORF Names:ipa-14r
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei111 – 131HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei147 – 167HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei177 – 197HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei209 – 229HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei245 – 265HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei288 – 308HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei329 – 349HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei360 – 380HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei388 – 408HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei412 – 432HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei434 – 454HelicalPROSITE-ProRule annotationAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001866921 – 459Negative regulator of SacY activityAdd BLAST459

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P15400

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224308.BSU38410

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P15400

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1 – 86PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST86
Domaini106 – 459PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST354

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The PTS EIIB type-2 domain may serve a regulatory function, through its phosphorylation activity.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1263, Bacteria
COG1264, Bacteria

Identification of Orthologs from Complete Genome Data

More...
OMAi
QGTFFRY

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P15400

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00212, PTS_IIB_glc, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.1360.60, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR036878, Glu_permease_IIB
IPR018113, PTrfase_EIIB_Cys
IPR003352, PTS_EIIC
IPR013013, PTS_EIIC_1
IPR001996, PTS_IIB_1
IPR010973, PTS_IIBC_sucr

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00367, PTS_EIIB, 1 hit
PF02378, PTS_EIIC, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55604, SSF55604, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01996, PTS-II-BC-sucr, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51098, PTS_EIIB_TYPE_1, 1 hit
PS01035, PTS_EIIB_TYPE_1_CYS, 1 hit
PS51103, PTS_EIIC_TYPE_1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P15400-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHKEIAKELL LLAGGKNNII SISHCTTRLR FDVKDETKID IHAIENLQGV
60 70 80 90 100
QGTFFRYGLF QIIFGAGVVN KIYKEVVHVW ETAPSEEPVH QKKASRKLNP
110 120 130 140 150
AAAFAKTLSD IFVPIIPAIT ASGLLMGLIG MIKVFHWFAA GSPWIKMLDL
160 170 180 190 200
VSSTAFILLP ILVGFSAARQ FGSNPYLGAV IAGLLTHPDL LDPSMLGSKT
210 220 230 240 250
PSSLDIWGLH IPMMGYQGSM IPILLSVFVM SKIEKLLKSI VPKSLDVVII
260 270 280 290 300
PFITVMVTGC LALIVMNPAA SIIGQIMTQS IVYIYDHAGI AAGALFGGIY
310 320 330 340 350
STIVLSGLHH SFYAIEATLL ANPHVGVNFL VPIWSMANVA QGGAGLAVFL
360 370 380 390 400
KTKQSSLKKI ALPASLTAFL GIVEPIVFGV NLKLIRPFIG AAIGGAIGGA
410 420 430 440 450
YVVAVQVVAN SYGLTGIPMI SIVLPFGAAN FVHYMIGFLI AAVSAFIATL

FLGFKEETE
Length:459
Mass (Da):49,024
Last modified:April 1, 1990 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA5C4E996ECDA3D40
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M29333 Genomic DNA Translation: AAA75335.1
X73124 Genomic DNA Translation: CAA51570.1
AL009126 Genomic DNA Translation: CAB15867.1
X52480 Genomic DNA Translation: CAA36719.1

Protein sequence database of the Protein Information Resource

More...
PIRi
JU0293

NCBI Reference Sequences

More...
RefSeqi
NP_391720.1, NC_000964.3
WP_003243800.1, NZ_JNCM01000034.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB15867; CAB15867; BSU_38410

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
937333

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU38410

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.4158

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29333 Genomic DNA Translation: AAA75335.1
X73124 Genomic DNA Translation: CAA51570.1
AL009126 Genomic DNA Translation: CAB15867.1
X52480 Genomic DNA Translation: CAA36719.1
PIRiJU0293
RefSeqiNP_391720.1, NC_000964.3
WP_003243800.1, NZ_JNCM01000034.1

3D structure databases

SMRiP15400
ModBaseiSearch...

Protein-protein interaction databases

STRINGi224308.BSU38410

Protein family/group databases

TCDBi4.A.1.2.10, the pts glucose-glucoside (glc) family

Proteomic databases

PaxDbiP15400

Genome annotation databases

EnsemblBacteriaiCAB15867; CAB15867; BSU_38410
GeneIDi937333
KEGGibsu:BSU38410
PATRICifig|224308.179.peg.4158

Phylogenomic databases

eggNOGiCOG1263, Bacteria
COG1264, Bacteria
OMAiQGTFFRY
PhylomeDBiP15400

Enzyme and pathway databases

BioCyciBSUB:BSU38410-MONOMER

Family and domain databases

CDDicd00212, PTS_IIB_glc, 1 hit
Gene3Di3.30.1360.60, 1 hit
InterProiView protein in InterPro
IPR036878, Glu_permease_IIB
IPR018113, PTrfase_EIIB_Cys
IPR003352, PTS_EIIC
IPR013013, PTS_EIIC_1
IPR001996, PTS_IIB_1
IPR010973, PTS_IIBC_sucr
PfamiView protein in Pfam
PF00367, PTS_EIIB, 1 hit
PF02378, PTS_EIIC, 1 hit
SUPFAMiSSF55604, SSF55604, 1 hit
TIGRFAMsiTIGR01996, PTS-II-BC-sucr, 1 hit
PROSITEiView protein in PROSITE
PS51098, PTS_EIIB_TYPE_1, 1 hit
PS01035, PTS_EIIB_TYPE_1_CYS, 1 hit
PS51103, PTS_EIIC_TYPE_1, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSACX_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P15400
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 29, 2021
This is version 154 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome
UniProt is an ELIXIR core data resource
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