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Entry version 201 (02 Jun 2021)
Sequence version 1 (01 Apr 1990)
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Protein

DNA topoisomerase 2

Gene

Top2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:8978614).

Topoisomerase II makes double-strand breaks (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:9545289).

Essential during mitosis and meiosis for proper segregation of daughter chromosomes (PubMed:10751154, PubMed:14600258, PubMed:18752348, PubMed:25340780).

During meiosis, it disrupts heterochromatic connections between achiasmate and chiasmate homologs after spindle assembly so that chromosomes can separate at prometaphase I (PubMed:25340780).

During mitosis, it functions in the separation of sister chromatids by establishing amphitelic kinetochore attachments in mitotic spindles (PubMed:18752348).

May have a role in chromatin condensation and chromosome structure (PubMed:14600258, PubMed:18752348, PubMed:25340780).

May be involved in X-chromosome dosage compensation, perhaps by modifying the topological state of compensated genes (PubMed:23989663).

Regulates activity of the gypsy chromatin insulator complex by binding to mod(mdg4) and preventing its degradation (PubMed:21304601).

13 Publications

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+4 Publications, Mn2+PROSITE-ProRule annotation, Ca2+2 PublicationsNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.PROSITE-ProRule annotation

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=200 µM for negatively supercoiled double-stranded plasmid DNA (at 30 degrees Celsius)1 Publication
  2. KM=140 µM for negatively supercoiled double-stranded viral DNA (at 30 degrees Celsius)1 Publication
  3. KM=270 µM for positively supercoiled double-stranded linear DNA1 Publication
  4. KM=280 µM for ATP (at 30 degrees Celsius)1 Publication
  5. KM=630 µM for dATP (at 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei72ATPBy similarity1
    Binding sitei101ATPBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi441Magnesium 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi521Magnesium 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi521Magnesium 2PROSITE-ProRule annotation1
    Metal bindingi523Magnesium 2PROSITE-ProRule annotation1
    <p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei784Transition state stabilizerBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei785O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1
    Sitei836Important for DNA bending; intercalates between base pairs of target DNABy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi129 – 131ATPBy similarity3
    Nucleotide bindingi142 – 149ATPBy similarity8
    Nucleotide bindingi357 – 359ATPBy similarity3

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionDNA-binding, Isomerase, Topoisomerase
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    Reactome - a knowledgebase of biological pathways and processes

    More...
    Reactomei
    R-DME-4615885, SUMOylation of DNA replication proteins

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    P15348

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    DNA topoisomerase 2Curated (EC:5.6.2.27 Publications)
    Alternative name(s):
    DNA topoisomerase IICurated
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:Top21 PublicationImported
    ORF Names:CG10223Imported
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2L

    Organism-specific databases

    Drosophila genome database

    More...
    FlyBasei
    FBgn0284220, Top2

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Nucleus

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Homozygous lethal (PubMed:21304601). Embryogenesis appears normal, probably due to maternal contribution of the protein (PubMed:21304601). However after hatching flies show a 2-3 day delay in development and most die before the third instar stage (PubMed:21304601). Conditional RNAi-mediated knockdown in the female germline does not affect fertilization but embryos fail to initiate proper mitotic divisions and do not hatch (PubMed:25340780). Embryos contain only two nuclei; a small nucleus with a centriolar spindle and a large round nucleus (PubMed:25340780). Knockdown in stage 3 oocytes often results in the heterochromatic regions of all four chromosomes failing to separate during prometaphase I and metaphase I of meiosis I (PubMed:25340780). In some instances, the anchored heterochromatic regions become stretched as the centromeres attempt to move towards the spindles poles creating long, abnormal heterochromatin structures that project from the main chromosome mass with centromeres at their tips (PubMed:25340780). Spindle assembly is not affected (PubMed:25340780). Spindles are bipolar although one or both sides of the spindle are elongated due to the abnormal heterochromatin projections (PubMed:25340780).2 Publications

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi359K → Q or E: No effect on double-stranded DNA cleavage. Strong decrease in ATPase activity and strand passage activity. 1 Publication1
    Mutagenesisi359K → R: No effect on enzyme activity. 1 Publication1

    Chemistry databases

    ChEMBL database of bioactive drug-like small molecules

    More...
    ChEMBLi
    CHEMBL2671

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001453741 – 1447DNA topoisomerase 2Add BLAST1447

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1284Phosphoserine1 Publication1
    Modified residuei1344Phosphoserine1 Publication1
    Modified residuei1352Phosphothreonine1 Publication1
    Modified residuei1374Phosphoserine1 Publication1
    Modified residuei1385Phosphoserine1 Publication1
    Modified residuei1392Phosphoserine1 Publication1
    Modified residuei1396Phosphoserine1 Publication1

    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

    Phosphorylated (PubMed:1328202, PubMed:8383533, PubMed:10751154). Phosphorylation by casein kinase II enhances ATPase activity (PubMed:1328202).3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P15348

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P15348

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...
    iPTMneti
    P15348

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

    Detected in germline and follicle cells (at protein level). Predominantly expressed in follicle cells where expression persists throughout oogenesis (at protein level). In germline cells, expression levels decrease as cells develop and move to the posterior region of the germarium.1 Publication

    Gene expression databases

    Bgee dataBase for Gene Expression Evolution

    More...
    Bgeei
    FBgn0003732, Expressed in eye disc (Drosophila) and 33 other tissues

    Genevisible search portal to normalized and curated expression data from Genevestigator

    More...
    Genevisiblei
    P15348, DM

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer (By similarity).

    Interacts with mod(mdg4) (PubMed:21304601).

    Interacts with barr (PubMed:8978614, PubMed:11172718).

    Interacts with ph-p (PubMed:11172718).

    Interacts with mle; the interaction mediates association with the MSL dosage compensation complex (PubMed:23989663).

    By similarity4 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei469Interaction with DNAPROSITE-ProRule annotation1
    Sitei472Interaction with DNAPROSITE-ProRule annotation1
    Sitei641Interaction with DNAPROSITE-ProRule annotation1
    Sitei642Interaction with DNAPROSITE-ProRule annotation1
    Sitei703Interaction with DNAPROSITE-ProRule annotation1
    Sitei737Interaction with DNAPROSITE-ProRule annotation1
    Sitei743Interaction with DNAPROSITE-ProRule annotation1
    Sitei911Interaction with DNABy similarity1

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...
    BioGRIDi
    61203, 25 interactors

    Protein interaction database and analysis system

    More...
    IntActi
    P15348, 13 interactors

    Molecular INTeraction database

    More...
    MINTi
    P15348

    STRING: functional protein association networks

    More...
    STRINGi
    7227.FBpp0304598

    Chemistry databases

    BindingDB database of measured binding affinities

    More...
    BindingDBi
    P15348

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P15348

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini435 – 552ToprimPROSITE-ProRule annotationAdd BLAST118

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni323 – 325Interaction with DNABy similarity3
    Regioni972 – 981Interaction with DNABy similarity10
    Regioni1079 – 1110DisorderedSequence analysisAdd BLAST32
    Regioni1183 – 1231DisorderedSequence analysisAdd BLAST49
    Regioni1246 – 1447DisorderedSequence analysisAdd BLAST202

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1095 – 1110Basic and acidic residuesSequence analysisAdd BLAST16
    Compositional biasi1254 – 1284Basic and acidic residuesSequence analysisAdd BLAST31
    Compositional biasi1306 – 1364Basic and acidic residuesSequence analysisAdd BLAST59
    Compositional biasi1391 – 1415Basic and acidic residuesSequence analysisAdd BLAST25
    Compositional biasi1430 – 1447Acidic residuesSequence analysisAdd BLAST18

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the type II topoisomerase family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG0355, Eukaryota

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00940000168342

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_001935_1_0_1

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P15348

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    DCASARY

    Database of Orthologous Groups

    More...
    OrthoDBi
    117851at2759

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P15348

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd00187, TOP4c, 1 hit
    cd03365, TOPRIM_TopoIIA, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.10.268.10, 1 hit
    3.30.230.10, 1 hit
    3.30.565.10, 1 hit
    3.40.50.670, 1 hit
    3.90.199.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR003594, HATPase_C
    IPR036890, HATPase_C_sf
    IPR020568, Ribosomal_S5_D2-typ_fold
    IPR014721, Ribosomal_S5_D2-typ_fold_subgr
    IPR001241, Topo_IIA
    IPR013760, Topo_IIA-like_dom_sf
    IPR002205, Topo_IIA_A/C
    IPR013758, Topo_IIA_A/C_ab
    IPR013757, Topo_IIA_A_a_sf
    IPR013759, Topo_IIA_B_C
    IPR013506, Topo_IIA_bsu_dom2
    IPR018522, TopoIIA_CS
    IPR031660, TOPRIM_C
    IPR006171, TOPRIM_domain
    IPR034157, TOPRIM_TopoII

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00204, DNA_gyraseB, 1 hit
    PF00521, DNA_topoisoIV, 1 hit
    PF02518, HATPase_c, 1 hit
    PF01751, Toprim, 1 hit
    PF16898, TOPRIM_C, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00433, TOP2c, 1 hit
    SM00434, TOP4c, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF54211, SSF54211, 1 hit
    SSF55874, SSF55874, 1 hit
    SSF56719, SSF56719, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00177, TOPOISOMERASE_II, 1 hit
    PS50880, TOPRIM, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P15348-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MENGNKALSI EQMYQKKSQL EHILLRPDSY IGSVEFTKEL MWVYDNSQNR
    60 70 80 90 100
    MVQKEISFVP GLYKIFDEIL VNAADNKQRD KSMNTIKIDI DPERNMVSVW
    110 120 130 140 150
    NNGQGIPVTM HKEQKMYVPT MIFGHLLTSS NYNDDEKKVT GGRNGYGAKL
    160 170 180 190 200
    CNIFSTSFTV ETATREYKKS FKQTWGNNMG KASDVQIKDF NGTDYTRITF
    210 220 230 240 250
    SPDLAKFKMD RLDEDIVALM SRRAYDVAAS SKGVSVFLNG NKLGVRNFKD
    260 270 280 290 300
    YIDLHIKNTD DDSGPPIKIV HEVANERWEV ACCPSDRGFQ QVSFVNSIAT
    310 320 330 340 350
    YKGGRHVDHV VDNLIKQLLE VLKKKNKGGI NIKPFQVRNH LWVFVNCLIE
    360 370 380 390 400
    NPTFDSQTKE NMTLQQKGFG SKCTLSEKFI NNMSKSGIVE SVLAWAKFKA
    410 420 430 440 450
    QNDIAKTGGR KSSKIKGIPK LEDANEAGGK NSIKCTLILT EGDSAKSLAV
    460 470 480 490 500
    SGLGVIGRDL YGVFPLRGKL LNVREANFKQ LSENAEINNL CKIIGLQYKK
    510 520 530 540 550
    KYLTEDDLKT LRYGKVMIMT DQDQDGSHIK GLLINFIHTN WPELLRLPFL
    560 570 580 590 600
    EEFITPIVKA TKKNEELSFY SLPEFEEWKN DTANHHTYNI KYYKGLGTST
    610 620 630 640 650
    SKEAKEYFQD MDRHRILFKY DGSVDDESIV MAFSKKHIES RKVWLTNHMD
    660 670 680 690 700
    EVKRRKELGL PERYLYTKGT KSITYADFIN LELVLFSNAD NERSIPSLVD
    710 720 730 740 750
    GLKPGQRKVM FTCFKRNDKR EVKVAQLSGS VAEMSAYHHG EVSLQMTIVN
    760 770 780 790 800
    LAQNFVGANN INLLEPRGQF GTRLSGGKDC ASARYIFTIM SPLTRLIYHP
    810 820 830 840 850
    LDDPLLDYQV DDGQKIEPLW YLPIIPMVLV NGAEGIGTGW STKISNYNPR
    860 870 880 890 900
    EIMKNLRKMI NGQEPSVMHP WYKNFLGRME YVSDGRYIQT GNIQILSGNR
    910 920 930 940 950
    LEISELPVGV WTQNYKENVL EPLSNGTEKV KGIISEYREY HTDTTVRFVI
    960 970 980 990 1000
    SFAPGEFERI HAEEGGFYRV FKLTTTLSTN QMHAFDQNNC LRRFPTAIDI
    1010 1020 1030 1040 1050
    LKEYYKLRRE YYARRRDFLV GQLTAQADRL SDQARFILEK CEKKLVVENK
    1060 1070 1080 1090 1100
    QRKAMCDELL KRGYRPDPVK EWQRRIKMED AEQADEEDEE EEEAAPSVSS
    1110 1120 1130 1140 1150
    KAKKEKEVDP EKAFKKLTDV KKFDYLLGMS MWMLTEEKKN ELLKQRDTKL
    1160 1170 1180 1190 1200
    SELESLRKKT PEMLWLDDLD ALESKLNEVE EKERAEEQGI NLKTAKALKG
    1210 1220 1230 1240 1250
    QKSASAKGRK VKSMGGGAGA GDVFPDPDGE PVEFKITEEI IKKMAAAAKV
    1260 1270 1280 1290 1300
    AQAAKEPKKP KEPKEPKVKK EPKGKQIKAE PDASGDEVDE FDAMVEGGSK
    1310 1320 1330 1340 1350
    TSPKAKKAVV KKEPGEKKPR QKKENGGGLK QSKIDFSKAK AKKSDDDVEE
    1360 1370 1380 1390 1400
    VTPRAERPGR RQASKKIDYS SLFSDEEEDG GNVGSDDDGN ASDDDSPKRP
    1410 1420 1430 1440
    AKRGREDESS GGAKKKAPPK KRRAVIESDD DDIEIDEDDD DDSDFNC
    Length:1,447
    Mass (Da):164,396
    Last modified:April 1, 1990 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i63B5D2814AD06419
    GO

    <p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

    The sequence AAQ23558 differs from that shown. Reason: Frameshift.Curated

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AE014134 Genomic DNA Translation: AGB93115.1
    X61209 Genomic DNA Translation: CAA43523.1
    AE014134 Genomic DNA Translation: AAF53802.2
    BT010240 mRNA Translation: AAQ23558.1 Frameshift.
    BT150455 mRNA Translation: AJP62077.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    S02160

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_001260580.1, NM_001273651.1
    NP_476760.1, NM_057412.5

    Genome annotation databases

    Ensembl metazoan genome annotation project

    More...
    EnsemblMetazoai
    FBtr0081287; FBpp0080825; FBgn0284220
    FBtr0332320; FBpp0304598; FBgn0284220

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    35225

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    dme:Dmel_CG10223

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE014134 Genomic DNA Translation: AGB93115.1
    X61209 Genomic DNA Translation: CAA43523.1
    AE014134 Genomic DNA Translation: AAF53802.2
    BT010240 mRNA Translation: AAQ23558.1 Frameshift.
    BT150455 mRNA Translation: AJP62077.1
    PIRiS02160
    RefSeqiNP_001260580.1, NM_001273651.1
    NP_476760.1, NM_057412.5

    3D structure databases

    SMRiP15348
    ModBaseiSearch...

    Protein-protein interaction databases

    BioGRIDi61203, 25 interactors
    IntActiP15348, 13 interactors
    MINTiP15348
    STRINGi7227.FBpp0304598

    Chemistry databases

    BindingDBiP15348
    ChEMBLiCHEMBL2671

    PTM databases

    iPTMnetiP15348

    Proteomic databases

    PaxDbiP15348
    PRIDEiP15348

    Genome annotation databases

    EnsemblMetazoaiFBtr0081287; FBpp0080825; FBgn0284220
    FBtr0332320; FBpp0304598; FBgn0284220
    GeneIDi35225
    KEGGidme:Dmel_CG10223

    Organism-specific databases

    Comparative Toxicogenomics Database

    More...
    CTDi
    35225
    FlyBaseiFBgn0284220, Top2

    Phylogenomic databases

    eggNOGiKOG0355, Eukaryota
    GeneTreeiENSGT00940000168342
    HOGENOMiCLU_001935_1_0_1
    InParanoidiP15348
    OMAiDCASARY
    OrthoDBi117851at2759
    PhylomeDBiP15348

    Enzyme and pathway databases

    ReactomeiR-DME-4615885, SUMOylation of DNA replication proteins
    SABIO-RKiP15348

    Miscellaneous databases

    ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

    More...
    ChiTaRSi
    Top2, fly

    Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

    More...
    GenomeRNAii
    35225

    Protein Ontology

    More...
    PROi
    PR:P15348

    Gene expression databases

    BgeeiFBgn0003732, Expressed in eye disc (Drosophila) and 33 other tissues
    GenevisibleiP15348, DM

    Family and domain databases

    CDDicd00187, TOP4c, 1 hit
    cd03365, TOPRIM_TopoIIA, 1 hit
    Gene3Di1.10.268.10, 1 hit
    3.30.230.10, 1 hit
    3.30.565.10, 1 hit
    3.40.50.670, 1 hit
    3.90.199.10, 1 hit
    InterProiView protein in InterPro
    IPR003594, HATPase_C
    IPR036890, HATPase_C_sf
    IPR020568, Ribosomal_S5_D2-typ_fold
    IPR014721, Ribosomal_S5_D2-typ_fold_subgr
    IPR001241, Topo_IIA
    IPR013760, Topo_IIA-like_dom_sf
    IPR002205, Topo_IIA_A/C
    IPR013758, Topo_IIA_A/C_ab
    IPR013757, Topo_IIA_A_a_sf
    IPR013759, Topo_IIA_B_C
    IPR013506, Topo_IIA_bsu_dom2
    IPR018522, TopoIIA_CS
    IPR031660, TOPRIM_C
    IPR006171, TOPRIM_domain
    IPR034157, TOPRIM_TopoII
    PfamiView protein in Pfam
    PF00204, DNA_gyraseB, 1 hit
    PF00521, DNA_topoisoIV, 1 hit
    PF02518, HATPase_c, 1 hit
    PF01751, Toprim, 1 hit
    PF16898, TOPRIM_C, 1 hit
    SMARTiView protein in SMART
    SM00433, TOP2c, 1 hit
    SM00434, TOP4c, 1 hit
    SUPFAMiSSF54211, SSF54211, 1 hit
    SSF55874, SSF55874, 1 hit
    SSF56719, SSF56719, 1 hit
    PROSITEiView protein in PROSITE
    PS00177, TOPOISOMERASE_II, 1 hit
    PS50880, TOPRIM, 1 hit

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTOP2_DROME
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P15348
    Secondary accession number(s): M9PDQ1, Q6NR40, Q9VIW2
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: June 2, 2021
    This is version 201 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families
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