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Protein

Embryonic polarity protein dorsal

Gene

dl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Embryonic developmental protein (PubMed:2598266, PubMed:10072776). The lateral or ventral identity of a cell depends upon the concentration of this protein in its nucleus during the blastoderm stage (PubMed:2598266). A morphogenetic protein that specifically binds to the kappa B-related consensus sequence 5'-GRGAAAANCC-3', located in the enhancer region of zygotic genes such as Zen, Twist, Snail and Decapentaplegic. Mediates an immune response in larvae (PubMed:10072776). Part of a signaling pathway involving NF-kappa-B and Toll-related receptors, that functions in the apoptosis of unfit cells during cell competition (PubMed:25477468). May be part of a NF-kappa-B and Tollo signaling cascade that regulates development of the peripheral nervous system (PubMed:18000549).4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-1810476 RIP-mediated NFkB activation via ZBP1
R-DME-202424 Downstream TCR signaling
R-DME-209400 Transcriptional activtion by phosphorylated DL/DIF dimer
R-DME-209406 Degradation of NF-kappa-B inhibitor, CACT
R-DME-209560 NF-kB is activated and signals survival
R-DME-214842 DL and DIF homodimers bind to TUB and phosphorylated PLL in the TL receptor 'signalling complex'
R-DME-214844 DL and DIF homodimers complexed with CACT are all phosphorylated in the TL receptor 'signalling complex'
R-DME-214869 Phosphorylated CACT, DL and DIF homodimers dissociate from the TL receptor 'signalling complex'
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-3134963 DEx/H-box helicases activate type I IFN and inflammatory cytokines production
R-DME-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-DME-4755510 SUMOylation of immune response proteins
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-5621575 CD209 (DC-SIGN) signaling
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-9020702 Interleukin-1 signaling
R-DME-933542 TRAF6 mediated NF-kB activation
SignaLinkiP15330

Names & Taxonomyi

Protein namesi
Recommended name:
Embryonic polarity protein dorsal
Gene namesi
Name:dl
ORF Names:CG6667
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0260632 dl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002051641 – 999Embryonic polarity protein dorsalAdd BLAST999

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei312Phosphoserine; by PKASequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP15330
PRIDEiP15330

PTM databases

iPTMnetiP15330

Expressioni

Tissue specificityi

In unchallenged larvae, expression of both isoforms is seen in fat body and gut (isoform A is more abundant). After immune challenge levels of both isoforms are enhanced.1 Publication

Developmental stagei

Isoform A is expressed maternally and both isoforms are expressed zygotically from 6-9 hours embryos through to adulthood.1 Publication

Gene expression databases

BgeeiFBgn0260632 Expressed in 28 organ(s), highest expression level in crop (Drosophila)
ExpressionAtlasiP15330 baseline and differential
GenevisibleiP15330 DM

Interactioni

Subunit structurei

Interacts with tamo via the nuclear localization signal. Interacts with emb, a component of the nuclear pore complex.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi61043, 70 interactors
DIPiDIP-17423N
IntActiP15330, 7 interactors
STRINGi7227.FBpp0080560

Structurei

3D structure databases

ProteinModelPortaliP15330
SMRiP15330
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 342RHDPROSITE-ProRule annotationAdd BLAST296

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi756 – 773Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi395 – 464Gln-richAdd BLAST70
Compositional biasi635 – 705Pro-richAdd BLAST71

Phylogenomic databases

eggNOGiENOG410IG8F Eukaryota
ENOG410XT64 LUCA
GeneTreeiENSGT00500000044765
InParanoidiP15330
KOiK09254
OMAiFNTLPRQ
OrthoDBiEOG091G07RW
PhylomeDBiP15330

Family and domain databases

CDDicd01177 IPT_NFkappaB, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.340, 1 hit
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR033926 IPT_NFkappaB
IPR000451 NFkB/Dor
IPR008967 p53-like_TF_DNA-bd
IPR030492 RHD_CS
IPR032397 RHD_dimer
IPR011539 RHD_DNA_bind_dom
IPR037059 RHD_DNA_bind_dom_sf
PANTHERiPTHR24169 PTHR24169, 2 hits
PfamiView protein in Pfam
PF16179 RHD_dimer, 1 hit
PF00554 RHD_DNA_bind, 1 hit
PRINTSiPR00057 NFKBTNSCPFCT
SMARTiView protein in SMART
SM00429 IPT, 1 hit
SUPFAMiSSF49417 SSF49417, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS01204 REL_1, 1 hit
PS50254 REL_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform C (identifier: P15330-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFPNQNNGAA PGQGPAVDGQ QSLNYNGLPA QQQQQLAQST KNVRKKPYVK
60 70 80 90 100
ITEQPAGKAL RFRYECEGRS AGSIPGVNST PENKTYPTIE IVGYKGRAVV
110 120 130 140 150
VVSCVTKDTP YRPHPHNLVG KEGCKKGVCT LEINSETMRA VFSNLGIQCV
160 170 180 190 200
KKKDIEAALK AREEIRVDPF KTGFSHRFQP SSIDLNSVRL CFQVFMESEQ
210 220 230 240 250
KGRFTSPLPP VVSEPIFDKK AMSDLVICRL CSCSATVFGN TQIILLCEKV
260 270 280 290 300
AKEDISVRFF EEKNGQSVWE AFGDFQHTDV HKQTAITFKT PRYHTLDITE
310 320 330 340 350
PAKVFIQLRR PSDGVTSEAL PFEYVPMDSG KHTFWNLHRH LKRKPDEDLF
360 370 380 390 400
QQILRLDAKR EVQPPTIEVI DLDTPKIDVQ REIPSEMEFN HEESQQSEPA
410 420 430 440 450
LEQEQSVQQE QYTQEQSLQQ EQYTQEQSLQ QEQYLQQLEQ QQSFQLEEPM
460 470 480 490 500
QQDQELPAQQ SFDQAIDHLP DHTSDHIPED MEAADAHAEA EAHRLRSEQE
510 520 530 540 550
KEIDTIIDEK VRELEQLDLG QQLEPRPLTA NDKITEWMKS SEIEQQVHEP
560 570 580 590 600
SPTAEADVLD SALEISKADK TLDELLETVA ELDEIYTDFK VQRDTYKNTI
610 620 630 640 650
QNELAGLQGR APLQVEDSFD DAATYTSLQI AFKNPVLIPM DDIMPPTPPM
660 670 680 690 700
SQCAPEDAHQ HYDPVEVNSQ ARKPETPMRP VPPVPPAILT IQYPPEEDKL
710 720 730 740 750
PPLPPKRIRK QDSNAENRSI EANTVQTKPS TGESPLNKRL PPAPKNPNFN
760 770 780 790 800
TLPRQKKPGF FSKLFSRRKS KPDLAQGQEN SSILDSKANS REPSIGHFNM
810 820 830 840 850
QDPMRASLRS SKSAAPFISN PAPAKSSPVK AKKPGSKLTK PVGRSVSSVS
860 870 880 890 900
GKRPAYLNAD VVHIPLKGDS VNSLPQQQRT EGYSQSSTIS VGAGLDRRTA
910 920 930 940 950
SALQLADIPI SEGGMELVAI ADRQSLHNLV SSIEGHFNVQ LDPNLDLTEA
960 970 980 990
EHFALYTSIP PLAAASEFDE TSAYYAPVDA GEILTPDEVA KRLAAANGI
Length:999
Mass (Da):111,551
Last modified:June 1, 2001 - v2
Checksum:iE29C6594AC07D662
GO
Isoform A (identifier: P15330-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     330-677: GKHTFWNLHR...NSQARKPETP → DPAHLRRKRQ...LQISNLSIST
     678-999: Missing.

Note: Nuclear localization signal at positions 335-340.Curated
Show »
Length:677
Mass (Da):75,347
Checksum:i10FEFCEB1D3A5789
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E1JHK3E1JHK3_DROME
Dorsal, isoform D
dl anon-EST:GressD7, d, DL, Dl, dL
677Annotation score:
C7LA93C7LA93_DROME
Dorsal, isoform F
dl anon-EST:GressD7, d, DL, Dl, dL
999Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236T → S in AAT94434 (Ref. 6) Curated1
Sequence conflicti391H → Q in AAC35296 (PubMed:10072776).Curated1
Sequence conflicti401L → F in AAC35296 (PubMed:10072776).Curated1
Sequence conflicti407V → SQQEQYTQEQSL in AAC35296 (PubMed:10072776).Curated1
Sequence conflicti698 – 699DK → EQ in AAC35296 (PubMed:10072776).Curated2
Sequence conflicti965 – 999ASEFD…AANGI → PVNLTRPPPTMLPWMLARF in AAC35296 (PubMed:10072776).CuratedAdd BLAST35
Isoform A (identifier: P15330-2)
Sequence conflicti506Q → QQ in AAA28479 (PubMed:3118464).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005581330 – 677GKHTF…KPETP → DPAHLRRKRQKTGGDPMHLL LQQQQKQQLQNDHQDGRQTN MNCWNTQNIPPIKTEPRDTS PQPFGLSYRAPPELTPSPQP LSPSSNYNHNSTPSPYNMAS AVTPTNGQQQLMSPNHPQQQ QQQQQYGATDLGSNYNPFAQ QVLAQQQQHQQQQQQHQHQH QQQHQQQQQQQQQQQQQSLQ FHANPFGNPGGNSWESKFSA AAVAAAAATATGAAPANGNS NNLSNLNNPFTMHNLLTSGG GPGNANNLQWNLTTNHLHNQ HTLHQQQQLQQQQQQQYDNT APTNNNANLNNNNNNNNTAG NQADNNGPTLSNLLSFDSGQ LVHINSEDQQILRLNSEDLQ ISNLSIST in isoform A. 3 PublicationsAdd BLAST348
Alternative sequenceiVSP_005582678 – 999Missing in isoform A. 3 PublicationsAdd BLAST322

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23702 mRNA Translation: AAA28479.1
AF053614 mRNA Translation: AAC35296.1
AE014134 Genomic DNA Translation: AAF53611.1
AE014134 Genomic DNA Translation: AAF53612.1
BT015205 mRNA Translation: AAT94434.1
PIRiA30350
RefSeqiNP_001163000.1, NM_001169529.1 [P15330-2]
NP_001163001.1, NM_001169530.1 [P15330-2]
NP_001286014.1, NM_001299085.1 [P15330-1]
NP_724052.1, NM_165217.3 [P15330-2]
NP_724053.1, NM_165218.3 [P15330-2]
NP_724054.1, NM_165219.2 [P15330-1]
UniGeneiDm.3233

Genome annotation databases

EnsemblMetazoaiFBtr0081005; FBpp0080558; FBgn0260632 [P15330-2]
FBtr0081006; FBpp0080559; FBgn0260632 [P15330-2]
FBtr0081007; FBpp0080560; FBgn0260632 [P15330-1]
FBtr0301383; FBpp0290597; FBgn0260632 [P15330-2]
FBtr0301384; FBpp0290598; FBgn0260632 [P15330-2]
FBtr0340250; FBpp0309222; FBgn0260632 [P15330-1]
GeneIDi35047
KEGGidme:Dmel_CG6667
UCSCiCG6667-RA d. melanogaster [P15330-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23702 mRNA Translation: AAA28479.1
AF053614 mRNA Translation: AAC35296.1
AE014134 Genomic DNA Translation: AAF53611.1
AE014134 Genomic DNA Translation: AAF53612.1
BT015205 mRNA Translation: AAT94434.1
PIRiA30350
RefSeqiNP_001163000.1, NM_001169529.1 [P15330-2]
NP_001163001.1, NM_001169530.1 [P15330-2]
NP_001286014.1, NM_001299085.1 [P15330-1]
NP_724052.1, NM_165217.3 [P15330-2]
NP_724053.1, NM_165218.3 [P15330-2]
NP_724054.1, NM_165219.2 [P15330-1]
UniGeneiDm.3233

3D structure databases

ProteinModelPortaliP15330
SMRiP15330
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61043, 70 interactors
DIPiDIP-17423N
IntActiP15330, 7 interactors
STRINGi7227.FBpp0080560

PTM databases

iPTMnetiP15330

Proteomic databases

PaxDbiP15330
PRIDEiP15330

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081005; FBpp0080558; FBgn0260632 [P15330-2]
FBtr0081006; FBpp0080559; FBgn0260632 [P15330-2]
FBtr0081007; FBpp0080560; FBgn0260632 [P15330-1]
FBtr0301383; FBpp0290597; FBgn0260632 [P15330-2]
FBtr0301384; FBpp0290598; FBgn0260632 [P15330-2]
FBtr0340250; FBpp0309222; FBgn0260632 [P15330-1]
GeneIDi35047
KEGGidme:Dmel_CG6667
UCSCiCG6667-RA d. melanogaster [P15330-1]

Organism-specific databases

CTDi35047
FlyBaseiFBgn0260632 dl

Phylogenomic databases

eggNOGiENOG410IG8F Eukaryota
ENOG410XT64 LUCA
GeneTreeiENSGT00500000044765
InParanoidiP15330
KOiK09254
OMAiFNTLPRQ
OrthoDBiEOG091G07RW
PhylomeDBiP15330

Enzyme and pathway databases

ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-1810476 RIP-mediated NFkB activation via ZBP1
R-DME-202424 Downstream TCR signaling
R-DME-209400 Transcriptional activtion by phosphorylated DL/DIF dimer
R-DME-209406 Degradation of NF-kappa-B inhibitor, CACT
R-DME-209560 NF-kB is activated and signals survival
R-DME-214842 DL and DIF homodimers bind to TUB and phosphorylated PLL in the TL receptor 'signalling complex'
R-DME-214844 DL and DIF homodimers complexed with CACT are all phosphorylated in the TL receptor 'signalling complex'
R-DME-214869 Phosphorylated CACT, DL and DIF homodimers dissociate from the TL receptor 'signalling complex'
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-3134963 DEx/H-box helicases activate type I IFN and inflammatory cytokines production
R-DME-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-DME-4755510 SUMOylation of immune response proteins
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-5621575 CD209 (DC-SIGN) signaling
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-9020702 Interleukin-1 signaling
R-DME-933542 TRAF6 mediated NF-kB activation
SignaLinkiP15330

Miscellaneous databases

ChiTaRSiDl fly
GenomeRNAii35047
PROiPR:P15330

Gene expression databases

BgeeiFBgn0260632 Expressed in 28 organ(s), highest expression level in crop (Drosophila)
ExpressionAtlasiP15330 baseline and differential
GenevisibleiP15330 DM

Family and domain databases

CDDicd01177 IPT_NFkappaB, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.340, 1 hit
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR033926 IPT_NFkappaB
IPR000451 NFkB/Dor
IPR008967 p53-like_TF_DNA-bd
IPR030492 RHD_CS
IPR032397 RHD_dimer
IPR011539 RHD_DNA_bind_dom
IPR037059 RHD_DNA_bind_dom_sf
PANTHERiPTHR24169 PTHR24169, 2 hits
PfamiView protein in Pfam
PF16179 RHD_dimer, 1 hit
PF00554 RHD_DNA_bind, 1 hit
PRINTSiPR00057 NFKBTNSCPFCT
SMARTiView protein in SMART
SM00429 IPT, 1 hit
SUPFAMiSSF49417 SSF49417, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS01204 REL_1, 1 hit
PS50254 REL_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDORS_DROME
AccessioniPrimary (citable) accession number: P15330
Secondary accession number(s): O77088
, Q0E8P9, Q6AWP3, Q9VJD9, Q9VJE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 209 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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