UniProtKB - P15330 (DORS_DROME)
Embryonic polarity protein dorsal
dl
Functioni
Embryonic developmental protein (PubMed:2598266, PubMed:10072776).
The lateral or ventral identity of a cell depends upon the concentration of this protein in its nucleus during the blastoderm stage (PubMed:2598266).
A morphogenetic protein that specifically binds to the kappa B-related consensus sequence 5'-GRGAAAANCC-3', located in the enhancer region of zygotic genes such as Zen, Twist, Snail and Decapentaplegic. Mediates an immune response in larvae (PubMed:10072776).
Part of a signaling pathway involving NF-kappa-B and Toll-related receptors, that functions in the apoptosis of unfit cells during cell competition (PubMed:25477468).
May be part of a NF-kappa-B and Tollo signaling cascade that regulates development of the peripheral nervous system (PubMed:18000549).
4 PublicationsGO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: FlyBase
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: FlyBase
- DNA-binding transcription factor binding Source: FlyBase
- high mobility group box 1 binding Source: FlyBase
- identical protein binding Source: IntAct
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: FlyBase
- sequence-specific DNA binding Source: FlyBase
GO - Biological processi
- dorsal/ventral axis specification Source: FlyBase
- dorsal/ventral pattern formation Source: FlyBase
- I-kappaB kinase/NF-kappaB signaling Source: GO_Central
- immune response Source: UniProtKB
- innate immune response Source: GO_Central
- melanization defense response Source: FlyBase
- NIK/NF-kappaB signaling Source: GO_Central
- peripheral nervous system neuron development Source: FlyBase
- positive regulation of antimicrobial peptide production Source: FlyBase
- positive regulation of hemocyte differentiation Source: FlyBase
- positive regulation of hemocyte proliferation Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- regulation of hemocyte proliferation Source: FlyBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to cytokine Source: GO_Central
- Toll signaling pathway Source: FlyBase
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DME-1169091, Activation of NF-kappaB in B cells R-DME-1810476, RIP-mediated NFkB activation via ZBP1 R-DME-202424, Downstream TCR signaling R-DME-209400, Transcriptional activtion by phosphorylated DL/DIF dimer R-DME-209406, Degradation of NF-kappa-B inhibitor, CACT R-DME-209560, NF-kB is activated and signals survival R-DME-214842, DL and DIF homodimers bind to TUB and phosphorylated PLL in the TL receptor 'signalling complex' R-DME-214844, DL and DIF homodimers complexed with CACT are all phosphorylated in the TL receptor 'signalling complex' R-DME-214869, Phosphorylated CACT, DL and DIF homodimers dissociate from the TL receptor 'signalling complex' R-DME-2871837, FCERI mediated NF-kB activation R-DME-3134963, DEx/H-box helicases activate type I IFN and inflammatory cytokines production R-DME-445989, TAK1 activates NFkB by phosphorylation and activation of IKKs complex R-DME-4755510, SUMOylation of immune response proteins R-DME-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-DME-5607764, CLEC7A (Dectin-1) signaling R-DME-5621575, CD209 (DC-SIGN) signaling R-DME-5676590, NIK-->noncanonical NF-kB signaling R-DME-6798695, Neutrophil degranulation R-DME-9020702, Interleukin-1 signaling R-DME-933542, TRAF6 mediated NF-kB activation |
SignaLinki | P15330 |
Names & Taxonomyi
Protein namesi | Recommended name: Embryonic polarity protein dorsal |
Gene namesi | Name:dl ORF Names:CG6667 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0260632, dl |
VEuPathDBi | VectorBase:FBgn0260632 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Cytoplasm and Cytosol
- Cytoplasm 3 Publications
Note: In ventral regions it is first cytoplasmic, then the protein is relocalized in the nucleus (PubMed:2598266). Its nuclear localization is essential to its function as a morphogen (PubMed:2598266). In dorsal regions it remains cytoplasmic (PubMed:2598266). Tamo negatively regulates nuclear import of dl (PubMed:12653959). Emb is responsible for export of dl from the nucleus (PubMed:14638854). Nuclear localization is enhanced upon microbial infection (PubMed:17032737).4 Publications
Nucleus
- Nucleus Curated
Cytosol
- cytosol Source: FlyBase
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- neuromuscular junction Source: FlyBase
- subsynaptic reticulum Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205164 | 1 – 999 | Embryonic polarity protein dorsalAdd BLAST | 999 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 312 | Phosphoserine; by PKASequence analysis | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P15330 |
PRIDEi | P15330 |
PTM databases
iPTMneti | P15330 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | FBgn0260632, Expressed in crop (Drosophila) and 37 other tissues |
ExpressionAtlasi | P15330, baseline and differential |
Genevisiblei | P15330, DM |
Interactioni
Subunit structurei
Binary interactionsi
P15330
GO - Molecular functioni
- DNA-binding transcription factor binding Source: FlyBase
- high mobility group box 1 binding Source: FlyBase
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 61043, 74 interactors |
DIPi | DIP-17423N |
IntActi | P15330, 7 interactors |
STRINGi | 7227.FBpp0080560 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 47 – 342 | RHDPROSITE-ProRule annotationAdd BLAST | 296 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 44 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 389 – 424 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 670 – 851 | DisorderedSequence analysisAdd BLAST | 182 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 668 – 677 | Nuclear export signal1 Publication | 10 | |
Motifi | 756 – 773 | Nuclear localization signalSequence analysisAdd BLAST | 18 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 42 | Polar residuesSequence analysisAdd BLAST | 42 | |
Compositional biasi | 399 – 424 | Polar residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 676 – 690 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 700 – 716 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 717 – 734 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 775 – 796 | Polar residuesSequence analysisAdd BLAST | 22 |
Phylogenomic databases
eggNOGi | ENOG502QQS6, Eukaryota |
GeneTreei | ENSGT00940000171058 |
HOGENOMi | CLU_016080_0_0_1 |
InParanoidi | P15330 |
OMAi | IPMEDIM |
PhylomeDBi | P15330 |
Family and domain databases
CDDi | cd01177, IPT_NFkappaB, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 2.60.40.340, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR002909, IPT_dom IPR033926, IPT_NFkappaB IPR000451, NFkB/Dor IPR008967, p53-like_TF_DNA-bd IPR030492, RHD_CS IPR032397, RHD_dimer IPR011539, RHD_DNA_bind_dom IPR037059, RHD_DNA_bind_dom_sf |
PANTHERi | PTHR24169, PTHR24169, 1 hit |
Pfami | View protein in Pfam PF16179, RHD_dimer, 1 hit PF00554, RHD_DNA_bind, 1 hit |
PRINTSi | PR00057, NFKBTNSCPFCT |
SMARTi | View protein in SMART SM00429, IPT, 1 hit |
SUPFAMi | SSF49417, SSF49417, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS01204, REL_1, 1 hit PS50254, REL_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFPNQNNGAA PGQGPAVDGQ QSLNYNGLPA QQQQQLAQST KNVRKKPYVK
60 70 80 90 100
ITEQPAGKAL RFRYECEGRS AGSIPGVNST PENKTYPTIE IVGYKGRAVV
110 120 130 140 150
VVSCVTKDTP YRPHPHNLVG KEGCKKGVCT LEINSETMRA VFSNLGIQCV
160 170 180 190 200
KKKDIEAALK AREEIRVDPF KTGFSHRFQP SSIDLNSVRL CFQVFMESEQ
210 220 230 240 250
KGRFTSPLPP VVSEPIFDKK AMSDLVICRL CSCSATVFGN TQIILLCEKV
260 270 280 290 300
AKEDISVRFF EEKNGQSVWE AFGDFQHTDV HKQTAITFKT PRYHTLDITE
310 320 330 340 350
PAKVFIQLRR PSDGVTSEAL PFEYVPMDSG KHTFWNLHRH LKRKPDEDLF
360 370 380 390 400
QQILRLDAKR EVQPPTIEVI DLDTPKIDVQ REIPSEMEFN HEESQQSEPA
410 420 430 440 450
LEQEQSVQQE QYTQEQSLQQ EQYTQEQSLQ QEQYLQQLEQ QQSFQLEEPM
460 470 480 490 500
QQDQELPAQQ SFDQAIDHLP DHTSDHIPED MEAADAHAEA EAHRLRSEQE
510 520 530 540 550
KEIDTIIDEK VRELEQLDLG QQLEPRPLTA NDKITEWMKS SEIEQQVHEP
560 570 580 590 600
SPTAEADVLD SALEISKADK TLDELLETVA ELDEIYTDFK VQRDTYKNTI
610 620 630 640 650
QNELAGLQGR APLQVEDSFD DAATYTSLQI AFKNPVLIPM DDIMPPTPPM
660 670 680 690 700
SQCAPEDAHQ HYDPVEVNSQ ARKPETPMRP VPPVPPAILT IQYPPEEDKL
710 720 730 740 750
PPLPPKRIRK QDSNAENRSI EANTVQTKPS TGESPLNKRL PPAPKNPNFN
760 770 780 790 800
TLPRQKKPGF FSKLFSRRKS KPDLAQGQEN SSILDSKANS REPSIGHFNM
810 820 830 840 850
QDPMRASLRS SKSAAPFISN PAPAKSSPVK AKKPGSKLTK PVGRSVSSVS
860 870 880 890 900
GKRPAYLNAD VVHIPLKGDS VNSLPQQQRT EGYSQSSTIS VGAGLDRRTA
910 920 930 940 950
SALQLADIPI SEGGMELVAI ADRQSLHNLV SSIEGHFNVQ LDPNLDLTEA
960 970 980 990
EHFALYTSIP PLAAASEFDE TSAYYAPVDA GEILTPDEVA KRLAAANGI
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC7LA93 | C7LA93_DROME | Dorsal, isoform F | dl anon-EST:GressD7, d, DL, Dl, dL | 999 | Annotation score: | ||
E1JHK3 | E1JHK3_DROME | Dorsal, isoform D | dl anon-EST:GressD7, d, DL, Dl, dL | 677 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 236 | T → S in AAT94434 (Ref. 6) Curated | 1 | ||
Sequence conflicti | 391 | H → Q in AAC35296 (PubMed:10072776).Curated | 1 | ||
Sequence conflicti | 401 | L → F in AAC35296 (PubMed:10072776).Curated | 1 | ||
Sequence conflicti | 407 | V → SQQEQYTQEQSL in AAC35296 (PubMed:10072776).Curated | 1 | ||
Sequence conflicti | 698 – 699 | DK → EQ in AAC35296 (PubMed:10072776).Curated | 2 | ||
Sequence conflicti | 965 – 999 | ASEFD…AANGI → PVNLTRPPPTMLPWMLARF in AAC35296 (PubMed:10072776).CuratedAdd BLAST | 35 | ||
Isoform A (identifier: P15330-2) | |||||
Sequence conflicti | 506 | Q → QQ in AAA28479 (PubMed:3118464).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005581 | 330 – 677 | GKHTF…KPETP → DPAHLRRKRQKTGGDPMHLL LQQQQKQQLQNDHQDGRQTN MNCWNTQNIPPIKTEPRDTS PQPFGLSYRAPPELTPSPQP LSPSSNYNHNSTPSPYNMAS AVTPTNGQQQLMSPNHPQQQ QQQQQYGATDLGSNYNPFAQ QVLAQQQQHQQQQQQHQHQH QQQHQQQQQQQQQQQQQSLQ FHANPFGNPGGNSWESKFSA AAVAAAAATATGAAPANGNS NNLSNLNNPFTMHNLLTSGG GPGNANNLQWNLTTNHLHNQ HTLHQQQQLQQQQQQQYDNT APTNNNANLNNNNNNNNTAG NQADNNGPTLSNLLSFDSGQ LVHINSEDQQILRLNSEDLQ ISNLSIST in isoform A. 3 PublicationsAdd BLAST | 348 | |
Alternative sequenceiVSP_005582 | 678 – 999 | Missing in isoform A. 3 PublicationsAdd BLAST | 322 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23702 mRNA Translation: AAA28479.1 AF053614 mRNA Translation: AAC35296.1 AE014134 Genomic DNA Translation: AAF53611.1 AE014134 Genomic DNA Translation: AAF53612.1 BT015205 mRNA Translation: AAT94434.1 |
PIRi | A30350 |
RefSeqi | NP_001163000.1, NM_001169529.1 [P15330-2] NP_001163001.1, NM_001169530.1 [P15330-2] NP_001286014.1, NM_001299085.1 [P15330-1] NP_724052.1, NM_165217.3 [P15330-2] NP_724053.1, NM_165218.3 [P15330-2] NP_724054.1, NM_165219.2 [P15330-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0081005; FBpp0080558; FBgn0260632 [P15330-2] FBtr0081006; FBpp0080559; FBgn0260632 [P15330-2] FBtr0081007; FBpp0080560; FBgn0260632 [P15330-1] FBtr0301383; FBpp0290597; FBgn0260632 [P15330-2] FBtr0301384; FBpp0290598; FBgn0260632 [P15330-2] FBtr0340250; FBpp0309222; FBgn0260632 [P15330-1] |
GeneIDi | 35047 |
KEGGi | dme:Dmel_CG6667 |
UCSCi | CG6667-RA, d. melanogaster [P15330-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23702 mRNA Translation: AAA28479.1 AF053614 mRNA Translation: AAC35296.1 AE014134 Genomic DNA Translation: AAF53611.1 AE014134 Genomic DNA Translation: AAF53612.1 BT015205 mRNA Translation: AAT94434.1 |
PIRi | A30350 |
RefSeqi | NP_001163000.1, NM_001169529.1 [P15330-2] NP_001163001.1, NM_001169530.1 [P15330-2] NP_001286014.1, NM_001299085.1 [P15330-1] NP_724052.1, NM_165217.3 [P15330-2] NP_724053.1, NM_165218.3 [P15330-2] NP_724054.1, NM_165219.2 [P15330-1] |
3D structure databases
AlphaFoldDBi | P15330 |
SMRi | P15330 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 61043, 74 interactors |
DIPi | DIP-17423N |
IntActi | P15330, 7 interactors |
STRINGi | 7227.FBpp0080560 |
PTM databases
iPTMneti | P15330 |
Proteomic databases
PaxDbi | P15330 |
PRIDEi | P15330 |
Genome annotation databases
EnsemblMetazoai | FBtr0081005; FBpp0080558; FBgn0260632 [P15330-2] FBtr0081006; FBpp0080559; FBgn0260632 [P15330-2] FBtr0081007; FBpp0080560; FBgn0260632 [P15330-1] FBtr0301383; FBpp0290597; FBgn0260632 [P15330-2] FBtr0301384; FBpp0290598; FBgn0260632 [P15330-2] FBtr0340250; FBpp0309222; FBgn0260632 [P15330-1] |
GeneIDi | 35047 |
KEGGi | dme:Dmel_CG6667 |
UCSCi | CG6667-RA, d. melanogaster [P15330-1] |
Organism-specific databases
CTDi | 35047 |
FlyBasei | FBgn0260632, dl |
VEuPathDBi | VectorBase:FBgn0260632 |
Phylogenomic databases
eggNOGi | ENOG502QQS6, Eukaryota |
GeneTreei | ENSGT00940000171058 |
HOGENOMi | CLU_016080_0_0_1 |
InParanoidi | P15330 |
OMAi | IPMEDIM |
PhylomeDBi | P15330 |
Enzyme and pathway databases
Reactomei | R-DME-1169091, Activation of NF-kappaB in B cells R-DME-1810476, RIP-mediated NFkB activation via ZBP1 R-DME-202424, Downstream TCR signaling R-DME-209400, Transcriptional activtion by phosphorylated DL/DIF dimer R-DME-209406, Degradation of NF-kappa-B inhibitor, CACT R-DME-209560, NF-kB is activated and signals survival R-DME-214842, DL and DIF homodimers bind to TUB and phosphorylated PLL in the TL receptor 'signalling complex' R-DME-214844, DL and DIF homodimers complexed with CACT are all phosphorylated in the TL receptor 'signalling complex' R-DME-214869, Phosphorylated CACT, DL and DIF homodimers dissociate from the TL receptor 'signalling complex' R-DME-2871837, FCERI mediated NF-kB activation R-DME-3134963, DEx/H-box helicases activate type I IFN and inflammatory cytokines production R-DME-445989, TAK1 activates NFkB by phosphorylation and activation of IKKs complex R-DME-4755510, SUMOylation of immune response proteins R-DME-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-DME-5607764, CLEC7A (Dectin-1) signaling R-DME-5621575, CD209 (DC-SIGN) signaling R-DME-5676590, NIK-->noncanonical NF-kB signaling R-DME-6798695, Neutrophil degranulation R-DME-9020702, Interleukin-1 signaling R-DME-933542, TRAF6 mediated NF-kB activation |
SignaLinki | P15330 |
Miscellaneous databases
ChiTaRSi | Dl, fly |
GenomeRNAii | 35047 |
PROi | PR:P15330 |
Gene expression databases
Bgeei | FBgn0260632, Expressed in crop (Drosophila) and 37 other tissues |
ExpressionAtlasi | P15330, baseline and differential |
Genevisiblei | P15330, DM |
Family and domain databases
CDDi | cd01177, IPT_NFkappaB, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 2.60.40.340, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR002909, IPT_dom IPR033926, IPT_NFkappaB IPR000451, NFkB/Dor IPR008967, p53-like_TF_DNA-bd IPR030492, RHD_CS IPR032397, RHD_dimer IPR011539, RHD_DNA_bind_dom IPR037059, RHD_DNA_bind_dom_sf |
PANTHERi | PTHR24169, PTHR24169, 1 hit |
Pfami | View protein in Pfam PF16179, RHD_dimer, 1 hit PF00554, RHD_DNA_bind, 1 hit |
PRINTSi | PR00057, NFKBTNSCPFCT |
SMARTi | View protein in SMART SM00429, IPT, 1 hit |
SUPFAMi | SSF49417, SSF49417, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS01204, REL_1, 1 hit PS50254, REL_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DORS_DROME | |
Accessioni | P15330Primary (citable) accession number: P15330 Secondary accession number(s): O77088 Q9VJE0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 230 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase