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Protein

Interferon regulatory factor 1

Gene

Irf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. These include the regulation of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Its target genes for transcriptional activation activity are: genes involved in anti-viral response, such as IFN-alpha/beta, DDX58/RIG-I, TNFSF10/TRAIL, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and RSAD2/viperin; antibacterial response, such as NOS2/INOS; anti-proliferative response, such as p53/TP53, LOX and CDKN1A; apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ/POLH; MHC class I expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and B2M and MHC class II expression, such as CIITA. Represses genes involved in anti-proliferative response, such as BIRC5/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells.6 Publications

Activity regulationi

Activated by MYD88.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi5 – 113IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST109

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processAntiviral defense, Immunity, Innate immunity, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 1
Short name:
IRF-1
Gene namesi
Name:Irf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96590 Irf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001545461 – 329Interferon regulatory factor 1Add BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78N6-acetyllysineBy similarity1
Cross-linki276Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki300Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Phosphorylated by CK2 and this positively regulates its activity.By similarity
Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-276. Sumoylation is enhanced by PIAS3. Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites (By similarity).By similarity
Ubiquitinated. Appears to compete with sumoylation on C-terminal sites (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP15314
PaxDbiP15314
PRIDEiP15314

PTM databases

iPTMnetiP15314
PhosphoSitePlusiP15314

Expressioni

Inductioni

By viruses and IFN-gamma.

Gene expression databases

BgeeiENSMUSG00000018899 Expressed in 280 organ(s), highest expression level in subcutaneous adipose tissue
CleanExiMM_IRF1
ExpressionAtlasiP15314 baseline and differential
GenevisibleiP15314 MM

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with EP300 (By similarity). Interacts with MYD88 and PIAS3.By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi200784, 5 interactors
DIPiDIP-61281N
IntActiP15314, 6 interactors
STRINGi10090.ENSMUSP00000019043

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP15314
SMRiP15314
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15314

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IER3 Eukaryota
ENOG4111HM7 LUCA
GeneTreeiENSGT00760000119093
HOGENOMiHOG000037937
HOVERGENiHBG003455
InParanoidiP15314
KOiK09444
OMAiCKEEPEV
OrthoDBiEOG091G0CB8
PhylomeDBiP15314
TreeFamiTF328512

Family and domain databases

CDDicd00103 IRF, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR019817 Interferon_reg_fac_CS
IPR001346 Interferon_reg_fact_DNA-bd_dom
IPR031215 IRF1
IPR017431 IRF1/IRF2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11949 PTHR11949, 1 hit
PTHR11949:SF3 PTHR11949:SF3, 1 hit
PfamiView protein in Pfam
PF00605 IRF, 1 hit
PIRSFiPIRSF038196 IFN_RF1/2, 1 hit
PRINTSiPR00267 INTFRNREGFCT
SMARTiView protein in SMART
SM00348 IRF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00601 IRF_1, 1 hit
PS51507 IRF_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

P15314-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPITRMRMRP WLEMQINSNQ IPGLIWINKE EMIFQIPWKH AAKHGWDINK
60 70 80 90 100
DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN
110 120 130 140 150
KGSSAVRVYR MLPPLTRNQR KERKSKSSRD TKSKTKRKLC GDVSPDTFSD
160 170 180 190 200
GLSSSTLPDD HSSYTTQGYL GQDLDMERDI TPALSPCVVS SSLSEWHMQM
210 220 230 240 250
DIIPDSTTDL YNLQVSPMPS TSEAATDEDE EGKIAEDLMK LFEQSEWQPT
260 270 280 290 300
HIDGKGYLLN EPGTQLSSVY GDFSCKEEPE IDSPRGDIGI GIQHVFTEMK
310 320
NMDSIMWMDS LLGNSVRLPP SIQAIPCAP
Length:329
Mass (Da):37,319
Last modified:April 1, 1990 - v1
Checksum:i0E5DD23C0D977B34
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3U5M1Q3U5M1_MOUSE
Interferon regulatory factor
Irf1
304Annotation score:
Q5SX15Q5SX15_MOUSE
Interferon regulatory factor 1
Irf1
132Annotation score:
Q5SX14Q5SX14_MOUSE
Interferon regulatory factor 1
Irf1
182Annotation score:
Q5SX16Q5SX16_MOUSE
Interferon regulatory factor 1
Irf1
62Annotation score:
A8Y5B9A8Y5B9_MOUSE
Interferon regulatory factor 1
Irf1
199Annotation score:
D6RH71D6RH71_MOUSE
Interferon regulatory factor 1
Irf1
51Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21065 mRNA Translation: AAA39334.1
BC003821 mRNA Translation: AAH03821.1
CCDSiCCDS24686.1
PIRiA31595
RefSeqiNP_001152868.1, NM_001159396.1
NP_032416.1, NM_008390.2
UniGeneiMm.105218

Genome annotation databases

EnsembliENSMUST00000019043; ENSMUSP00000019043; ENSMUSG00000018899
ENSMUST00000108920; ENSMUSP00000104548; ENSMUSG00000018899
GeneIDi16362
KEGGimmu:16362
UCSCiuc007iww.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21065 mRNA Translation: AAA39334.1
BC003821 mRNA Translation: AAH03821.1
CCDSiCCDS24686.1
PIRiA31595
RefSeqiNP_001152868.1, NM_001159396.1
NP_032416.1, NM_008390.2
UniGeneiMm.105218

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IF1X-ray3.00A/B1-113[»]
ProteinModelPortaliP15314
SMRiP15314
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200784, 5 interactors
DIPiDIP-61281N
IntActiP15314, 6 interactors
STRINGi10090.ENSMUSP00000019043

PTM databases

iPTMnetiP15314
PhosphoSitePlusiP15314

Proteomic databases

EPDiP15314
PaxDbiP15314
PRIDEiP15314

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019043; ENSMUSP00000019043; ENSMUSG00000018899
ENSMUST00000108920; ENSMUSP00000104548; ENSMUSG00000018899
GeneIDi16362
KEGGimmu:16362
UCSCiuc007iww.2 mouse

Organism-specific databases

CTDi3659
MGIiMGI:96590 Irf1

Phylogenomic databases

eggNOGiENOG410IER3 Eukaryota
ENOG4111HM7 LUCA
GeneTreeiENSGT00760000119093
HOGENOMiHOG000037937
HOVERGENiHBG003455
InParanoidiP15314
KOiK09444
OMAiCKEEPEV
OrthoDBiEOG091G0CB8
PhylomeDBiP15314
TreeFamiTF328512

Miscellaneous databases

EvolutionaryTraceiP15314
PROiPR:P15314
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018899 Expressed in 280 organ(s), highest expression level in subcutaneous adipose tissue
CleanExiMM_IRF1
ExpressionAtlasiP15314 baseline and differential
GenevisibleiP15314 MM

Family and domain databases

CDDicd00103 IRF, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR019817 Interferon_reg_fac_CS
IPR001346 Interferon_reg_fact_DNA-bd_dom
IPR031215 IRF1
IPR017431 IRF1/IRF2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11949 PTHR11949, 1 hit
PTHR11949:SF3 PTHR11949:SF3, 1 hit
PfamiView protein in Pfam
PF00605 IRF, 1 hit
PIRSFiPIRSF038196 IFN_RF1/2, 1 hit
PRINTSiPR00267 INTFRNREGFCT
SMARTiView protein in SMART
SM00348 IRF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00601 IRF_1, 1 hit
PS51507 IRF_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIRF1_MOUSE
AccessioniPrimary (citable) accession number: P15314
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 7, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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