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Protein

Ezrin

Gene

EZR

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.3 Publications

Activity regulationi

A head-to-tail association, of the N-terminal and C-terminal halves results in a closed conformation (inactive form) which is incapable of actin or membrane-binding.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell shape

Enzyme and pathway databases

ReactomeiR-HSA-373752 Netrin-1 signaling
R-HSA-437239 Recycling pathway of L1
SIGNORiP15311

Names & Taxonomyi

Protein namesi
Recommended name:
Ezrin
Alternative name(s):
Cytovillin
Villin-2
p81
Gene namesi
Name:EZR
Synonyms:VIL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000092820.17
HGNCiHGNC:12691 EZR
MIMi123900 gene
neXtProtiNX_P15311

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi7430
MalaCardsiEZR
OpenTargetsiENSG00000092820
PharmGKBiPA162385512

Chemistry databases

ChEMBLiCHEMBL1932896

Polymorphism and mutation databases

BioMutaiEZR
DMDMi125987826

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194081 – 586EzrinAdd BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60N6-acetyllysineCombined sources1
Modified residuei146Phosphotyrosine; by PDGFR2 Publications1
Modified residuei354Phosphotyrosine; by PDGFR1 Publication1
Modified residuei366PhosphoserineCombined sources1
Modified residuei478Phosphotyrosine1 Publication1
Modified residuei535PhosphoserineBy similarity1
Modified residuei567Phosphothreonine; by ROCK2 and PKC/PRKCI1 Publication1

Post-translational modificationi

Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).By similarity
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

EPDiP15311
MaxQBiP15311
PaxDbiP15311
PeptideAtlasiP15311
PRIDEiP15311
ProteomicsDBi53128
TopDownProteomicsiP15311

2D gel databases

DOSAC-COBS-2DPAGEiP15311
REPRODUCTION-2DPAGEiIPI00843975
SWISS-2DPAGEiP15311

PTM databases

iPTMnetiP15311
PhosphoSitePlusiP15311
SwissPalmiP15311

Expressioni

Tissue specificityi

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.1 Publication

Developmental stagei

Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.1 Publication

Gene expression databases

BgeeiENSG00000092820 Expressed in 234 organ(s), highest expression level in nasal cavity epithelium
CleanExiHS_EZR
ExpressionAtlasiP15311 baseline and differential
GenevisibleiP15311 HS

Organism-specific databases

HPAiCAB004035
CAB047324
CAB075739
HPA021616

Interactioni

Subunit structurei

Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B (PubMed:9314537, PubMed:12651155, PubMed:15498789, PubMed:17616675, PubMed:17881735, PubMed:19555689). Interacts (when phosphorylated) with FES/FPS (PubMed:18046454). Interacts with dimeric S100P, the interaction may be activating through unmasking of F-actin binding sites (PubMed:12808036, PubMed:19111582). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Detected in a complex composed of at least EZR, AHNAK, PPL and PRX (By similarity). Interacts with PDPN (via cytoplasmic domain); activates RHOA and promotes epithelial-mesenchymal transition (PubMed:17046996). Interacts with SPN, CD44 and ICAM2 (By similarity).By similarity10 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113271, 153 interactors
CORUMiP15311
DIPiDIP-38868N
ELMiP15311
IntActiP15311, 205 interactors
MINTiP15311
STRINGi9606.ENSP00000338934

Chemistry databases

BindingDBiP15311

Structurei

Secondary structure

1586
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00775
ProteinModelPortaliP15311
SMRiP15311
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15311

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 295FERMPROSITE-ProRule annotationAdd BLAST294

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni244 – 586Interaction with SCYL31 PublicationAdd BLAST343

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi115 – 120[IL]-x-C-x-x-[DE] motif1 Publication6

Domaini

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.1 Publication

Phylogenomic databases

eggNOGiKOG3529 Eukaryota
ENOG410XQFP LUCA
GeneTreeiENSGT00920000148976
HOGENOMiHOG000007113
HOVERGENiHBG002185
InParanoidiP15311
KOiK08007
PhylomeDBiP15311
TreeFamiTF313935

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
1.25.40.1020, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR019749 Band_41_domain
IPR011174 ERM
IPR011259 ERM_C_dom
IPR000798 Ez/rad/moesin-like
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR019747 FERM_CS
IPR000299 FERM_domain
IPR018979 FERM_N
IPR018980 FERM_PH-like_C
IPR008954 Moesin_tail_sf
IPR011993 PH-like_dom_sf
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00769 ERM, 1 hit
PF09380 FERM_C, 1 hit
PF00373 FERM_M, 1 hit
PF09379 FERM_N, 1 hit
PIRSFiPIRSF002305 ERM, 1 hit
PRINTSiPR00935 BAND41
PR00661 ERMFAMILY
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM01196 FERM_C, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF48678 SSF48678, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00660 FERM_1, 1 hit
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P15311-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPKPINVRVT TMDAELEFAI QPNTTGKQLF DQVVKTIGLR EVWYFGLHYV
60 70 80 90 100
DNKGFPTWLK LDKKVSAQEV RKENPLQFKF RAKFYPEDVA EELIQDITQK
110 120 130 140 150
LFFLQVKEGI LSDEIYCPPE TAVLLGSYAV QAKFGDYNKE VHKSGYLSSE
160 170 180 190 200
RLIPQRVMDQ HKLTRDQWED RIQVWHAEHR GMLKDNAMLE YLKIAQDLEM
210 220 230 240 250
YGINYFEIKN KKGTDLWLGV DALGLNIYEK DDKLTPKIGF PWSEIRNISF
260 270 280 290 300
NDKKFVIKPI DKKAPDFVFY APRLRINKRI LQLCMGNHEL YMRRRKPDTI
310 320 330 340 350
EVQQMKAQAR EEKHQKQLER QQLETEKKRR ETVEREKEQM MREKEELMLR
360 370 380 390 400
LQDYEEKTKK AERELSEQIQ RALQLEEERK RAQEEAERLE ADRMAALRAK
410 420 430 440 450
EELERQAVDQ IKSQEQLAAE LAEYTAKIAL LEEARRRKED EVEEWQHRAK
460 470 480 490 500
EAQDDLVKTK EELHLVMTAP PPPPPPVYEP VSYHVQESLQ DEGAEPTGYS
510 520 530 540 550
AELSSEGIRD DRNEEKRITE AEKNERVQRQ LLTLSSELSQ ARDENKRTHN
560 570 580
DIIHNENMRQ GRDKYKTLRQ IRQGNTKQRI DEFEAL
Length:586
Mass (Da):69,413
Last modified:February 6, 2007 - v4
Checksum:iF1B592CF49A7CC46
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7EQR4E7EQR4_HUMAN
Ezrin
EZR
586Annotation score:

Sequence cautioni

The sequence AAA61278 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB82418 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030572180R → C. Corresponds to variant dbSNP:rs3103004EnsemblClinVar.1
Natural variantiVAR_030573494A → P. Corresponds to variant dbSNP:rs2230143Ensembl.1
Natural variantiVAR_015112532L → V2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51521 mRNA Translation: CAA35893.1
J05021 mRNA Translation: AAA61278.1 Different initiation.
AL162086 mRNA Translation: CAB82418.1 Different initiation.
AL589931 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47647.1
BC013903 mRNA Translation: AAH13903.1
CCDSiCCDS5258.1
PIRiA34400
RefSeqiNP_001104547.1, NM_001111077.1
NP_003370.2, NM_003379.4
UniGeneiHs.487027

Genome annotation databases

EnsembliENST00000337147; ENSP00000338934; ENSG00000092820
ENST00000367075; ENSP00000356042; ENSG00000092820
GeneIDi7430
KEGGihsa:7430
UCSCiuc003qrt.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51521 mRNA Translation: CAA35893.1
J05021 mRNA Translation: AAA61278.1 Different initiation.
AL162086 mRNA Translation: CAB82418.1 Different initiation.
AL589931 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47647.1
BC013903 mRNA Translation: AAH13903.1
CCDSiCCDS5258.1
PIRiA34400
RefSeqiNP_001104547.1, NM_001111077.1
NP_003370.2, NM_003379.4
UniGeneiHs.487027

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NI2X-ray2.30A/B2-297[»]
4RM8X-ray1.90A/B1-586[»]
4RM9X-ray2.00A1-586[»]
4RMAX-ray1.75A/B1-296[»]
DisProtiDP00775
ProteinModelPortaliP15311
SMRiP15311
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113271, 153 interactors
CORUMiP15311
DIPiDIP-38868N
ELMiP15311
IntActiP15311, 205 interactors
MINTiP15311
STRINGi9606.ENSP00000338934

Chemistry databases

BindingDBiP15311
ChEMBLiCHEMBL1932896

PTM databases

iPTMnetiP15311
PhosphoSitePlusiP15311
SwissPalmiP15311

Polymorphism and mutation databases

BioMutaiEZR
DMDMi125987826

2D gel databases

DOSAC-COBS-2DPAGEiP15311
REPRODUCTION-2DPAGEiIPI00843975
SWISS-2DPAGEiP15311

Proteomic databases

EPDiP15311
MaxQBiP15311
PaxDbiP15311
PeptideAtlasiP15311
PRIDEiP15311
ProteomicsDBi53128
TopDownProteomicsiP15311

Protocols and materials databases

DNASUi7430
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337147; ENSP00000338934; ENSG00000092820
ENST00000367075; ENSP00000356042; ENSG00000092820
GeneIDi7430
KEGGihsa:7430
UCSCiuc003qrt.5 human

Organism-specific databases

CTDi7430
DisGeNETi7430
EuPathDBiHostDB:ENSG00000092820.17
GeneCardsiEZR
HGNCiHGNC:12691 EZR
HPAiCAB004035
CAB047324
CAB075739
HPA021616
MalaCardsiEZR
MIMi123900 gene
neXtProtiNX_P15311
OpenTargetsiENSG00000092820
PharmGKBiPA162385512
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3529 Eukaryota
ENOG410XQFP LUCA
GeneTreeiENSGT00920000148976
HOGENOMiHOG000007113
HOVERGENiHBG002185
InParanoidiP15311
KOiK08007
PhylomeDBiP15311
TreeFamiTF313935

Enzyme and pathway databases

ReactomeiR-HSA-373752 Netrin-1 signaling
R-HSA-437239 Recycling pathway of L1
SIGNORiP15311

Miscellaneous databases

ChiTaRSiEZR human
EvolutionaryTraceiP15311
GeneWikiiEzrin
GenomeRNAii7430
PROiPR:P15311
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000092820 Expressed in 234 organ(s), highest expression level in nasal cavity epithelium
CleanExiHS_EZR
ExpressionAtlasiP15311 baseline and differential
GenevisibleiP15311 HS

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
1.25.40.1020, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR019749 Band_41_domain
IPR011174 ERM
IPR011259 ERM_C_dom
IPR000798 Ez/rad/moesin-like
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR019747 FERM_CS
IPR000299 FERM_domain
IPR018979 FERM_N
IPR018980 FERM_PH-like_C
IPR008954 Moesin_tail_sf
IPR011993 PH-like_dom_sf
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00769 ERM, 1 hit
PF09380 FERM_C, 1 hit
PF00373 FERM_M, 1 hit
PF09379 FERM_N, 1 hit
PIRSFiPIRSF002305 ERM, 1 hit
PRINTSiPR00935 BAND41
PR00661 ERMFAMILY
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM01196 FERM_C, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF48678 SSF48678, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00660 FERM_1, 1 hit
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEZRI_HUMAN
AccessioniPrimary (citable) accession number: P15311
Secondary accession number(s): E1P5A8
, P23714, Q4VX75, Q96CU8, Q9NSJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 6, 2007
Last modified: October 10, 2018
This is version 210 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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