UniProtKB - P15274 (AMPD_YEAST)
Protein
AMP deaminase
Gene
AMD1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
AMP deaminase plays a critical role in energy metabolism.
Miscellaneous
Present with 3910 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.5.4.6
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
: IMP biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes IMP from AMP.Proteins known to be involved in this subpathway in this organism are:
- AMP deaminase (AMD1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from AMP, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 362 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 364 | Zinc; catalyticBy similarity | 1 | |
Binding sitei | 364 | SubstrateBy similarity | 1 | |
Metal bindingi | 630 | Zinc; catalyticBy similarity | 1 | |
Binding sitei | 633 | SubstrateBy similarity | 1 | |
Active sitei | 652 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 707 | Zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- AMP deaminase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- AMP metabolic process Source: GO_Central
- guanine salvage Source: SGD
- IMP biosynthetic process Source: GO_Central
- IMP salvage Source: UniProtKB-UniPathway
- purine nucleotide metabolic process Source: SGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-6798695, Neutrophil degranulation R-SCE-74217, Purine salvage |
UniPathwayi | UPA00591;UER00663 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:AMD1 Synonyms:AMD Ordered Locus Names:YML035C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004498, AMD1 |
VEuPathDBi | FungiDB:YML035C |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194414 | 1 – 810 | AMP deaminaseAdd BLAST | 810 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 19 | PhosphoserineCombined sources | 1 | |
Modified residuei | 58 | PhosphoserineCombined sources | 1 | |
Modified residuei | 61 | PhosphoserineCombined sources | 1 | |
Modified residuei | 138 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P15274 |
PaxDbi | P15274 |
PRIDEi | P15274 |
PTM databases
iPTMneti | P15274 |
Interactioni
Subunit structurei
Homotetramer.
Binary interactionsi
P15274
With | #Exp. | IntAct |
---|---|---|
PRP40 [P33203] | 2 | EBI-2548,EBI-701 |
Protein-protein interaction databases
BioGRIDi | 35135, 217 interactors |
DIPi | DIP-1949N |
IntActi | P15274, 11 interactors |
MINTi | P15274 |
STRINGi | 4932.YML035C |
Miscellaneous databases
RNActi | P15274, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 433 – 438 | Substrate bindingBy similarity | 6 | |
Regioni | 708 – 711 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.Curated
Phylogenomic databases
eggNOGi | KOG1096, Eukaryota |
GeneTreei | ENSGT00950000183011 |
HOGENOMi | CLU_003782_2_1_1 |
InParanoidi | P15274 |
OMAi | QHVEWRI |
Family and domain databases
CDDi | cd01319, AMPD, 1 hit |
InterProi | View protein in InterPro IPR006650, A/AMP_deam_AS IPR001365, A/AMP_deaminase_dom IPR006329, AMPD IPR032466, Metal_Hydrolase |
PANTHERi | PTHR11359, PTHR11359, 1 hit |
Pfami | View protein in Pfam PF00962, A_deaminase, 1 hit |
PIRSFi | PIRSF001251, AMP_deaminase_met, 1 hit |
SUPFAMi | SSF51556, SSF51556, 1 hit |
TIGRFAMsi | TIGR01429, AMP_deaminase, 1 hit |
PROSITEi | View protein in PROSITE PS00485, A_DEAMINASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P15274-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDNQATQRLN DLSLEPAPSH DEQDGSGLVI DIDQRKIGDE QAGVVVDDET
60 70 80 90 100
PPLEQQDSHE SLAADSRNAN FSYHENQQLL ENGTKQLALD EHDSHSAILE
110 120 130 140 150
QPSHSTNCSS SNIAAMNKGH DSADHASQNS GGKPRTLSAS AQHILPETLK
160 170 180 190 200
SFAGAPVVNK QVRTSASYKM GMLADDASQQ FLDDPSSELI DLYSKVAECR
210 220 230 240 250
NLRAKYQTIS VQNDDQNPKN KPGWVVYPPP PKPSYNSDTK TVVPVTNKPD
260 270 280 290 300
AEVFDFTKCE IPGEDPDWEF TLNDDDSYVV HRSGKTDELI AQIPTLRDYY
310 320 330 340 350
LDLEKMISIS SDGPAKSFAY RRLQYLEARW NLYYLLNEYQ ETSVSKRNPH
360 370 380 390 400
RDFYNVRKVD THVHHSACMN QKHLLRFIKH KLRHSKDEKV IFRDGKLLTL
410 420 430 440 450
DEVFRSLHLT GYDLSIDTLD MHAHKDTFHR FDKFNLKYNP IGESRLREIF
460 470 480 490 500
LKTNNYIKGT YLADITKQVI FDLENSKYQN CEYRISVYGR SLDEWDKLAS
510 520 530 540 550
WVIDNKVISH NVRWLVQIPR LYDIYKKTGI VQSFQDICKN LFQPLFEVTK
560 570 580 590 600
NPQSHPKLHV FLQRVIGFDS VDDESKVDRR FHRKYPKPSL WEAPQNPPYS
610 620 630 640 650
YYLYYLYSNV ASLNQWRAKR GFNTLVLRPH CGEAGDPEHL VSAYLLAHGI
660 670 680 690 700
SHGILLRKVP FVQYLYYLDQ VGIAMSPLSN NALFLTYDKN PFPRYFKRGL
710 720 730 740 750
NVSLSTDDPL QFSYTREPLI EEYSVAAQIY KLSNVDMCEL ARNSVLQSGW
760 770 780 790 800
EAQIKKHWIG KDFDKSGVEG NDVVRTNVPD IRINYRYDTL STELELVNHF
810
ANFKRTIEEK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 568 | F → C in AAA34420 (PubMed:2690949).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30449 Genomic DNA Translation: AAA34420.1 Z46659 Genomic DNA Translation: CAA86620.1 BK006946 Genomic DNA Translation: DAA09864.1 |
PIRi | S49744 |
RefSeqi | NP_013677.1, NM_001182392.1 |
Genome annotation databases
EnsemblFungii | YML035C_mRNA; YML035C; YML035C |
GeneIDi | 854973 |
KEGGi | sce:YML035C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30449 Genomic DNA Translation: AAA34420.1 Z46659 Genomic DNA Translation: CAA86620.1 BK006946 Genomic DNA Translation: DAA09864.1 |
PIRi | S49744 |
RefSeqi | NP_013677.1, NM_001182392.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 35135, 217 interactors |
DIPi | DIP-1949N |
IntActi | P15274, 11 interactors |
MINTi | P15274 |
STRINGi | 4932.YML035C |
PTM databases
iPTMneti | P15274 |
Proteomic databases
MaxQBi | P15274 |
PaxDbi | P15274 |
PRIDEi | P15274 |
Genome annotation databases
EnsemblFungii | YML035C_mRNA; YML035C; YML035C |
GeneIDi | 854973 |
KEGGi | sce:YML035C |
Organism-specific databases
SGDi | S000004498, AMD1 |
VEuPathDBi | FungiDB:YML035C |
Phylogenomic databases
eggNOGi | KOG1096, Eukaryota |
GeneTreei | ENSGT00950000183011 |
HOGENOMi | CLU_003782_2_1_1 |
InParanoidi | P15274 |
OMAi | QHVEWRI |
Enzyme and pathway databases
UniPathwayi | UPA00591;UER00663 |
Reactomei | R-SCE-6798695, Neutrophil degranulation R-SCE-74217, Purine salvage |
Miscellaneous databases
PROi | PR:P15274 |
RNActi | P15274, protein |
Family and domain databases
CDDi | cd01319, AMPD, 1 hit |
InterProi | View protein in InterPro IPR006650, A/AMP_deam_AS IPR001365, A/AMP_deaminase_dom IPR006329, AMPD IPR032466, Metal_Hydrolase |
PANTHERi | PTHR11359, PTHR11359, 1 hit |
Pfami | View protein in Pfam PF00962, A_deaminase, 1 hit |
PIRSFi | PIRSF001251, AMP_deaminase_met, 1 hit |
SUPFAMi | SSF51556, SSF51556, 1 hit |
TIGRFAMsi | TIGR01429, AMP_deaminase, 1 hit |
PROSITEi | View protein in PROSITE PS00485, A_DEAMINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPD_YEAST | |
Accessioni | P15274Primary (citable) accession number: P15274 Secondary accession number(s): D6VZE0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families