UniProtKB - P15257 (HNF1A_RAT)
Protein
Hepatocyte nuclear factor 1-alpha
Gene
Hnf1a
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (By similarity). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 199 – 279 | Homeobox; HNF1-typePROSITE-ProRule annotationAdd BLAST | 81 |
GO - Molecular functioni
- chromatin binding Source: Ensembl
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription factor activity Source: MGI
- double-stranded DNA binding Source: RGD
- protein heterodimerization activity Source: Ensembl
- protein homodimerization activity Source: Ensembl
- RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl
- sequence-specific DNA binding Source: RGD
- transcription cofactor binding Source: RGD
- transcription factor binding Source: RGD
- transcription regulatory region DNA binding Source: UniProtKB
GO - Biological processi
- bile acid and bile salt transport Source: Ensembl
- bile acid biosynthetic process Source: Ensembl
- blastocyst development Source: Ensembl
- bone resorption Source: Ensembl
- cholesterol metabolic process Source: Ensembl
- chromatin remodeling Source: Ensembl
- embryonic limb morphogenesis Source: Ensembl
- endocrine pancreas development Source: Ensembl
- fatty acid biosynthetic process Source: Ensembl
- fatty acid transport Source: Ensembl
- glucose homeostasis Source: Ensembl
- glucose import Source: Ensembl
- heme biosynthetic process Source: Ensembl
- histone acetylation Source: Ensembl
- insulin secretion Source: Ensembl
- liver development Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: RGD
- paraxial mesoderm formation Source: Ensembl
- placenta development Source: Ensembl
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of hormone secretion Source: Ensembl
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of Wnt signaling pathway Source: Ensembl
- renal glucose absorption Source: Ensembl
- response to glucose Source: Ensembl
- response to oxidative stress Source: Ensembl
- reverse cholesterol transport Source: Ensembl
- SMAD protein signal transduction Source: Ensembl
- transcription by RNA polymerase II Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Hepatocyte nuclear factor 1-alphaShort name: HNF-1-alpha Short name: HNF-1A Alternative name(s): Liver-specific transcription factor LF-B1 Short name: LFB1 Transcription factor 1 Short name: TCF-1 |
Gene namesi | Name:Hnf1a Synonyms:Hnf-1, Hnf-1a, Tcf1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3828 Hnf1a |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: RGD
- pronucleus Source: Ensembl
Other locations
- cytoplasm Source: Ensembl
- photoreceptor outer segment Source: Ensembl
- transcription factor complex Source: Ensembl
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049117 | 1 – 628 | Hepatocyte nuclear factor 1-alphaAdd BLAST | 628 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 70 | PhosphoserineBy similarity | 1 | |
Modified residuei | 74 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 93 | PhosphoserineBy similarity | 1 | |
Modified residuei | 247 | PhosphoserineCombined sources | 1 | |
Modified residuei | 313 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P15257 |
PRIDEi | P15257 |
PTM databases
iPTMneti | P15257 |
PhosphoSitePlusi | P15257 |
Expressioni
Tissue specificityi
Liver.
Gene expression databases
Bgeei | ENSRNOG00000001183 Expressed in 8 organ(s), highest expression level in liver |
Genevisiblei | P15257 RN |
Interactioni
Subunit structurei
Binds DNA as a dimer (PubMed:8491173). Heterotetramer with PCBD1; formed by a dimer of dimers (By similarity).
Interacts with PCBD1 (By similarity).
Interacts with BHLHE41 (By similarity).
By similarity1 PublicationGO - Molecular functioni
- protein heterodimerization activity Source: Ensembl
- protein homodimerization activity Source: Ensembl
- transcription cofactor binding Source: RGD
- transcription factor binding Source: RGD
Protein-protein interaction databases
BioGridi | 246939, 1 interactor |
STRINGi | 10116.ENSRNOP00000001565 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P15257 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P15257 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 31 | DimerizationAdd BLAST | 31 | |
Regioni | 130 – 132 | Interaction with DNABy similarity | 3 | |
Regioni | 143 – 149 | Interaction with DNABy similarity | 7 | |
Regioni | 155 – 158 | Interaction with DNABy similarity | 4 | |
Regioni | 203 – 206 | Interaction with DNABy similarity | 4 | |
Regioni | 263 – 265 | Interaction with DNABy similarity | 3 | |
Regioni | 270 – 273 | Interaction with DNABy similarity | 4 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 197 – 205 | Nuclear localization signalSequence analysis | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 71 – 80 | Asp/Glu-rich (acidic; potential involvement with transcription) | 10 |
Sequence similaritiesi
Belongs to the HNF1 homeobox family.Curated
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | ENOG410IFA0 Eukaryota ENOG410ZZZ0 LUCA |
GeneTreei | ENSGT00940000153818 |
HOGENOMi | HOG000015305 |
InParanoidi | P15257 |
KOi | K08036 |
OMAi | PEHGVKY |
OrthoDBi | 409935at2759 |
PhylomeDBi | P15257 |
TreeFami | TF320327 |
Family and domain databases
CDDi | cd00086 homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR039066 HNF-1 IPR006899 HNF-1_N IPR023219 HNF1_dimer_N_dom_sf IPR006898 HNF1a_C IPR006897 HNF1b_C IPR009057 Homeobox-like_sf IPR001356 Homeobox_dom IPR010982 Lambda_DNA-bd_dom_sf |
PANTHERi | PTHR11568 PTHR11568, 1 hit |
Pfami | View protein in Pfam PF04814 HNF-1_N, 1 hit PF04813 HNF-1A_C, 1 hit PF04812 HNF-1B_C, 1 hit |
SMARTi | View protein in SMART SM00389 HOX, 1 hit |
SUPFAMi | SSF100957 SSF100957, 1 hit SSF46689 SSF46689, 1 hit SSF47413 SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P15257-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSKLSQLQT ELLAALLESG LSKEALIQAL GEPGPYLMVG DGPLDKGESC
60 70 80 90 100
GGTRGDLTEL PNGLGETRGS EDDTDDDGED FAPPILKELE NLSPEEAAHQ
110 120 130 140 150
KAVVESLLQE DPWRVAKMVK SYLQQHNIPQ REVVDTTGLN QSHLSQHLNK
160 170 180 190 200
GTPMKTQKRA ALYTWYVRKQ REVAQQFTHA GQGGLIEEPT GDELPTKKGR
210 220 230 240 250
RNRFKWGPAS QQILFQAYER QKNPSKEERE TLVEECNRAE CIQRGVSPSQ
260 270 280 290 300
AQGLGSNLVT EVRVYNWFAN RRKEEAFRHK LAMDTYNGPP PGPGPGPALP
310 320 330 340 350
AHSSPGLPTT TLSPSKVHGV RYGQSATSEA AEVPSSSGGP LVTVSAALHQ
360 370 380 390 400
VSPTGLEPSS LLSTEAKLVS ATGGPLPPVS TLTALHSLEQ TSPGLNQQPQ
410 420 430 440 450
NLIMASLPGV MTIGPGEPAS LGPTFTNTGA STLVIGLAST QAQSVPVINS
460 470 480 490 500
MGSSLTTLQP VQFSQPLHPS YQQPLMPPVQ SHVAQSPFMA TMAQLQSPHA
510 520 530 540 550
LYSHKPEVAQ YTHTSLLPQT MLITDTNLST LASLTPTKQV FTSDTEASSE
560 570 580 590 600
PGLHEPSSPA TTIHIPSQDP SNIQHLQPAH RLSTSPTVSS SSLVLYQSSD
610 620
SNGHSHLLPS NHGVIETFIS TQMASSSQ
Sequence cautioni
The sequence CAA37387 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03170 mRNA Translation: AAA41524.1 X54423 mRNA Translation: CAA38295.1 X67649 Genomic DNA Translation: CAA47891.1 X53297 mRNA Translation: CAA37387.1 Different initiation. |
PIRi | A33333 S25485 |
RefSeqi | NP_036801.1, NM_012669.1 |
Genome annotation databases
Ensembli | ENSRNOT00000001565; ENSRNOP00000001565; ENSRNOG00000001183 |
GeneIDi | 24817 |
KEGGi | rno:24817 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03170 mRNA Translation: AAA41524.1 X54423 mRNA Translation: CAA38295.1 X67649 Genomic DNA Translation: CAA47891.1 X53297 mRNA Translation: CAA37387.1 Different initiation. |
PIRi | A33333 S25485 |
RefSeqi | NP_036801.1, NM_012669.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1LFB | X-ray | 2.80 | A | 195-286 | [»] | |
2LFB | NMR | - | A | 195-286 | [»] | |
SMRi | P15257 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 246939, 1 interactor |
STRINGi | 10116.ENSRNOP00000001565 |
PTM databases
iPTMneti | P15257 |
PhosphoSitePlusi | P15257 |
Proteomic databases
PaxDbi | P15257 |
PRIDEi | P15257 |
Genome annotation databases
Ensembli | ENSRNOT00000001565; ENSRNOP00000001565; ENSRNOG00000001183 |
GeneIDi | 24817 |
KEGGi | rno:24817 |
Organism-specific databases
CTDi | 6927 |
RGDi | 3828 Hnf1a |
Phylogenomic databases
eggNOGi | ENOG410IFA0 Eukaryota ENOG410ZZZ0 LUCA |
GeneTreei | ENSGT00940000153818 |
HOGENOMi | HOG000015305 |
InParanoidi | P15257 |
KOi | K08036 |
OMAi | PEHGVKY |
OrthoDBi | 409935at2759 |
PhylomeDBi | P15257 |
TreeFami | TF320327 |
Miscellaneous databases
EvolutionaryTracei | P15257 |
PROi | PR:P15257 |
Gene expression databases
Bgeei | ENSRNOG00000001183 Expressed in 8 organ(s), highest expression level in liver |
Genevisiblei | P15257 RN |
Family and domain databases
CDDi | cd00086 homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR039066 HNF-1 IPR006899 HNF-1_N IPR023219 HNF1_dimer_N_dom_sf IPR006898 HNF1a_C IPR006897 HNF1b_C IPR009057 Homeobox-like_sf IPR001356 Homeobox_dom IPR010982 Lambda_DNA-bd_dom_sf |
PANTHERi | PTHR11568 PTHR11568, 1 hit |
Pfami | View protein in Pfam PF04814 HNF-1_N, 1 hit PF04813 HNF-1A_C, 1 hit PF04812 HNF-1B_C, 1 hit |
SMARTi | View protein in SMART SM00389 HOX, 1 hit |
SUPFAMi | SSF100957 SSF100957, 1 hit SSF46689 SSF46689, 1 hit SSF47413 SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HNF1A_RAT | |
Accessioni | P15257Primary (citable) accession number: P15257 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | November 13, 2019 | |
This is version 188 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references