UniProtKB - P15254 (PUR4_ECOLI)
Protein
Phosphoribosylformylglycinamidine synthase
Gene
purL
Organism
Escherichia coli (strain K12)
Status
Functioni
Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotation1 Publication
Catalytic activityi
- ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-β-D-ribosyl)glycinamide = 2-formamido-N1-(5-O-phospho-β-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphateUniRule annotation1 PublicationEC:6.3.5.3UniRule annotation1 Publication
Kineticsi
- KM=64 µM for glutamine (at pH 7.25 and 37 degrees Celsius)1 Publication
- KM=51 µM for ATP (at pH 7.25 and 37 degrees Celsius)1 Publication
- KM=30 µM for FGAR (formylglycinamide ribonucleotide at pH 7.25 and 37 degrees Celsius)1 Publication
pH dependencei
Optimum pH is 7.2 with only 50% of this activity retains at pH 6.2 or 8.0.1 Publication
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Phosphoribosylformylglycinamidine synthase (purL), Phosphoribosylformylglycinamidine synthase (purL)
- Phosphoribosylformylglycinamidine cyclo-ligase (purM), Phosphoribosylformylglycinamidine cyclo-ligase (purM)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 678 | ATP; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 718 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 722 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 884 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 886 | ATPUniRule annotation | 1 | |
Active sitei | 1135 | NucleophileUniRule annotation | 1 | |
Active sitei | 1260 | UniRule annotation | 1 | |
Active sitei | 1262 | UniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 307 – 318 | ATPUniRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- ATPase activity Source: EcoliWiki
- ATP binding Source: EcoliWiki
- metal ion binding Source: UniProtKB-KW
- phosphoribosylformylglycinamidine synthase activity Source: EcoCyc
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: EcoliWiki
- glutamine metabolic process Source: EcoliWiki
Keywordsi
Molecular function | Ligase |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:FGAMSYN-MONOMER MetaCyc:FGAMSYN-MONOMER |
UniPathwayi | UPA00074;UER00128 |
Protein family/group databases
MEROPSi | C56.972 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoribosylformylglycinamidine synthaseUniRule annotation (EC:6.3.5.3UniRule annotation)Short name: FGAM synthaseUniRule annotation Short name: FGAMSUniRule annotation Alternative name(s): Formylglycinamide ribonucleotide amidotransferaseUniRule annotation Short name: FGAR amidotransferaseUniRule annotation Short name: FGAR-ATUniRule annotation |
Gene namesi | Name:purLUniRule annotation Synonyms:purI Ordered Locus Names:b2557, JW2541 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation1 Publication
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100406 | 1 – 1295 | Phosphoribosylformylglycinamidine synthaseAdd BLAST | 1295 |
Post-translational modificationi
Both N-terminus methionine truncation and retention have been observed for this protein.1 Publication
Proteomic databases
jPOSTi | P15254 |
PaxDbi | P15254 |
PRIDEi | P15254 |
Interactioni
Subunit structurei
Monomer.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4259206, 75 interactors |
DIPi | DIP-10614N |
IntActi | P15254, 14 interactors |
STRINGi | 511145.b2557 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1042 – 1295 | Glutamine amidotransferase type-1UniRule annotationAdd BLAST | 254 |
Sequence similaritiesi
In the N-terminal section; belongs to the FGAMS family.UniRule annotation
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | COG0046, Bacteria COG0047, Bacteria |
HOGENOMi | CLU_001031_0_2_6 |
InParanoidi | P15254 |
PhylomeDBi | P15254 |
Family and domain databases
Gene3Di | 3.30.1330.10, 2 hits 3.40.50.880, 1 hit 3.90.650.10, 2 hits |
HAMAPi | MF_00419, PurL_1, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR040707, FGAR-AT_N IPR017926, GATASE IPR010073, PurL_large IPR041609, PurL_linker IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR036921, PurM-like_N_sf IPR036604, PurS-like_sf |
Pfami | View protein in Pfam PF02769, AIRS_C, 2 hits PF18072, FGAR-AT_linker, 1 hit PF18076, FGAR-AT_N, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit SSF55326, SSF55326, 2 hits SSF56042, SSF56042, 2 hits SSF82697, SSF82697, 1 hit |
TIGRFAMsi | TIGR01735, FGAM_synt, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P15254-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMEILRGSPA LSAFRINKLL ARFQAARLPV HNIYAEYVHF ADLNAPLNDD
60 70 80 90 100
EHAQLERLLK YGPALASHAP QGKLLLVTPR PGTISPWSSK ATDIAHNCGL
110 120 130 140 150
QQVNRLERGV AYYIEAGTLT NEQWQQVTAE LHDRMMETVF FALDDAEQLF
160 170 180 190 200
AHHQPTPVTS VDLLGQGRQA LIDANLRLGL ALAEDEIDYL QDAFTKLGRN
210 220 230 240 250
PNDIELYMFA QANSEHCRHK IFNADWVIDG EQQPKSLFKM IKNTFETTPD
260 270 280 290 300
HVLSAYKDNA AVMEGSEVGR YFADHETGRY DFHQEPAHIL MKVETHNHPT
310 320 330 340 350
AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE
360 370 380 390 400
EDFGKPERIV TALDIMTEGP LGGAAFNNEF GRPALNGYFR TYEEKVNSHN
410 420 430 440 450
GEELRGYHKP IMLAGGIGNI RADHVQKGEI NVGAKLVVLG GPAMNIGLGG
460 470 480 490 500
GAASSMASGQ SDADLDFASV QRDNPEMERR CQEVIDRCWQ LGDANPILFI
510 520 530 540 550
HDVGAGGLSN AMPELVSDGG RGGKFELREI LSDEPGMSPL EIWCNESQER
560 570 580 590 600
YVLAVAADQL PLFDELCKRE RAPYAVIGEA TEELHLSLHD RHFDNQPIDL
610 620 630 640 650
PLDVLLGKTP KMTRDVQTLK AKGDALAREG ITIADAVKRV LHLPTVAEKT
660 670 680 690 700
FLVTIGDRSV TGMVARDQMV GPWQVPVANC AVTTASLDSY YGEAMAIGER
710 720 730 740 750
APVALLDFAA SARLAVGEAL TNIAATQIGD IKRIKLSANW MAAAGHPGED
760 770 780 790 800
AGLYEAVKAV GEELCPALGL TIPVGKDSMS MKTRWQEGNE EREMTSPLSL
810 820 830 840 850
VISAFARVED VRHTITPQLS TEDNALLLID LGKGNNALGA TALAQVYRQL
860 870 880 890 900
GDKPADVRDV AQLKGFYDAI QALVAQRKLL AYHDRSDGGL LVTLAEMAFA
910 920 930 940 950
GHCGIDADIA TLGDDRLAAL FNEELGAVIQ VRAADREAVE SVLAQHGLAD
960 970 980 990 1000
CVHYVGQAVS GDRFVITANG QTVFSESRTT LRVWWAETTW QMQRLRDNPE
1010 1020 1030 1040 1050
CADQEHQAKS NDADPGLNVK LSFDINEDVA APYIATGARP KVAVLREQGV
1060 1070 1080 1090 1100
NSHVEMAAAF HRAGFDAIDV HMSDLLTGRT GLEDFHALVA CGGFSYGDVL
1110 1120 1130 1140 1150
GAGEGWAKSI LFNDRVRDEF ATFFHRPQTL ALGVCNGCQM MSNLRELIPG
1160 1170 1180 1190 1200
SELWPRFVRN TSDRFEARFS LVEVTQSPSL LLQGMVGSQM PIAVSHGEGR
1210 1220 1230 1240 1250
VEVRDAAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE
1260 1270 1280 1290
SGRVTIMMPH PERVFRTVSN SWHPENWGED GPWMRIFRNA RKQLG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19501 Genomic DNA Translation: AAA24456.1 U36841 Genomic DNA Translation: AAA79819.1 U00096 Genomic DNA Translation: AAT48143.1 AP009048 Genomic DNA Translation: BAE76733.1 |
PIRi | D65033, SYECPG |
RefSeqi | WP_000970102.1, NZ_LN832404.1 YP_026170.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48143; AAT48143; b2557 BAE76733; BAE76733; BAE76733 |
GeneIDi | 947032 |
KEGGi | ecj:JW2541 eco:b2557 |
PATRICi | fig|1411691.4.peg.4177 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19501 Genomic DNA Translation: AAA24456.1 U36841 Genomic DNA Translation: AAA79819.1 U00096 Genomic DNA Translation: AAT48143.1 AP009048 Genomic DNA Translation: BAE76733.1 |
PIRi | D65033, SYECPG |
RefSeqi | WP_000970102.1, NZ_LN832404.1 YP_026170.1, NC_000913.3 |
3D structure databases
SMRi | P15254 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259206, 75 interactors |
DIPi | DIP-10614N |
IntActi | P15254, 14 interactors |
STRINGi | 511145.b2557 |
Protein family/group databases
MEROPSi | C56.972 |
Proteomic databases
jPOSTi | P15254 |
PaxDbi | P15254 |
PRIDEi | P15254 |
Genome annotation databases
EnsemblBacteriai | AAT48143; AAT48143; b2557 BAE76733; BAE76733; BAE76733 |
GeneIDi | 947032 |
KEGGi | ecj:JW2541 eco:b2557 |
PATRICi | fig|1411691.4.peg.4177 |
Organism-specific databases
EchoBASEi | EB0790 |
Phylogenomic databases
eggNOGi | COG0046, Bacteria COG0047, Bacteria |
HOGENOMi | CLU_001031_0_2_6 |
InParanoidi | P15254 |
PhylomeDBi | P15254 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00128 |
BioCyci | EcoCyc:FGAMSYN-MONOMER MetaCyc:FGAMSYN-MONOMER |
Miscellaneous databases
PROi | PR:P15254 |
Family and domain databases
Gene3Di | 3.30.1330.10, 2 hits 3.40.50.880, 1 hit 3.90.650.10, 2 hits |
HAMAPi | MF_00419, PurL_1, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR040707, FGAR-AT_N IPR017926, GATASE IPR010073, PurL_large IPR041609, PurL_linker IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR036921, PurM-like_N_sf IPR036604, PurS-like_sf |
Pfami | View protein in Pfam PF02769, AIRS_C, 2 hits PF18072, FGAR-AT_linker, 1 hit PF18076, FGAR-AT_N, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit SSF55326, SSF55326, 2 hits SSF56042, SSF56042, 2 hits SSF82697, SSF82697, 1 hit |
TIGRFAMsi | TIGR01735, FGAM_synt, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUR4_ECOLI | |
Accessioni | P15254Primary (citable) accession number: P15254 Secondary accession number(s): P78097, Q2MAH3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | December 4, 2007 | |
Last modified: | December 2, 2020 | |
This is version 183 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families