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UniProtKB - P15207 (ANDR_RAT)

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Protein

Androgen receptor

Gene

Ar

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3 (By similarity).By similarity2 Publications

Miscellaneous

In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone-receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.
Transcriptional activity is enhanced by binding to RANBP9.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei688AndrogenCombined sources1 Publication1
Binding sitei735AndrogenCombined sources1 Publication1
Binding sitei860AndrogenCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi541 – 614Nuclear receptorPROSITE-ProRule annotationAdd BLAST74
Zinc fingeri542 – 562NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri578 – 602NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

  • androgen binding Source: RGD
  • androgen receptor activity Source: RGD
  • androgen receptor binding Source: RGD
  • beta-catenin binding Source: UniProtKB
  • chromatin binding Source: RGD
  • DNA binding Source: RGD
  • DNA binding transcription factor activity Source: UniProtKB
  • nuclear receptor activity Source: BHF-UCL
  • protein domain specific binding Source: RGD
  • ribonucleotide binding Source: RGD
  • sequence-specific DNA binding Source: RGD
  • signaling receptor binding Source: UniProtKB
  • steroid binding Source: UniProtKB-KW
  • transcription factor binding Source: RGD
  • transcription regulatory region DNA binding Source: UniProtKB
  • zinc ion binding Source: InterPro
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • androgen receptor signaling pathway Source: RGD
  • cellular response to follicle-stimulating hormone stimulus Source: RGD
  • cellular response to steroid hormone stimulus Source: RGD
  • copulation Source: RGD
  • intracellular receptor signaling pathway Source: BHF-UCL
  • male courtship behavior Source: RGD
  • male sex differentiation Source: RGD
  • negative regulation of extrinsic apoptotic signaling pathway Source: BHF-UCL
  • negative regulation of integrin biosynthetic process Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: RGD
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • positive regulation of penile erection Source: RGD
  • positive regulation of phosphorylation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: RGD
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • prostate gland growth Source: RGD
  • regulation of protein localization to plasma membrane Source: UniProtKB
  • reproductive behavior Source: RGD
  • response to estradiol Source: RGD
  • response to insulin Source: RGD
  • response to testosterone Source: RGD
  • skeletal muscle hypertrophy Source: RGD
  • spermatogenesis Source: RGD
  • transcription, DNA-templated Source: UniProtKB
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionDNA-binding, Receptor
Biological processTranscription, Transcription regulation
LigandLipid-binding, Metal-binding, Steroid-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Androgen receptor
Alternative name(s):
Dihydrotestosterone receptor
Nuclear receptor subfamily 3 group C member 4
Gene namesi
Name:Ar
Synonyms:Nr3c4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2147 Ar

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Ar are a cause of androgen insensitivity. Rats with this syndrome are called testicular feminized (TFM).1 Publication

Keywords - Diseasei

Disease mutation

Chemistry databases

ChEMBLiCHEMBL3072
GuidetoPHARMACOLOGYi628

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537121 – 902Androgen receptorAdd BLAST902

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61Phosphoserine; by CDK9By similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei221Phosphotyrosine; by CSKBy similarity1
Modified residuei254PhosphoserineBy similarity1
Modified residuei265Phosphotyrosine; by CSK and TNK2By similarity1
Modified residuei290PhosphoserineCombined sources1
Modified residuei305Phosphotyrosine; by CSKBy similarity1
Modified residuei344Phosphotyrosine; by CSKBy similarity1
Modified residuei355Phosphotyrosine; by CSKBy similarity1
Modified residuei360Phosphotyrosine; by CSKBy similarity1
Modified residuei361Phosphotyrosine; by CSK and TNK2By similarity1
Cross-linki384Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei391Phosphotyrosine; by CSKBy similarity1
Cross-linki503Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei517Phosphotyrosine; by CSKBy similarity1
Modified residuei534Phosphotyrosine; by CSKBy similarity1
Modified residuei633PhosphoserineCombined sources1
Cross-linki828Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki830Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei898Phosphotyrosine; by CSKBy similarity1

Post-translational modificationi

Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-517 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression (By similarity). Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity. Phosphorylation at Ser-61 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition (By similarity).By similarity
Sumoylated on Lys-384 (major) and Lys-503 (By similarity). Ubiquitinated. Deubiquitinated by USP26 (By similarity). 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity (By similarity).By similarity
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP15207
PRIDEiP15207

PTM databases

iPTMnetiP15207
PhosphoSitePlusiP15207

Expressioni

Tissue specificityi

Highest levels in the seminal vesicle, ventral prostate and coagulating gland with lower levels in the kidney and levator ani muscle.

Interactioni

Subunit structurei

Binds DNA as a homodimer. Part of a ternary complex containing AR, EFCAB6/DJBP and PARK7. Interacts with HIPK3 and NR0B2 in the presence of androgen. The ligand binding domain interacts with KAT7/HBO1 in the presence of dihydrotestosterone. Interacts with EFCAB6/DJBP, PELP1, PQBP1, RANBP9, RBAK, SPDEF, SRA1, TGFB1I1, ZNF318 and RREB1. Interacts with ZMIZ1/ZIMP10 and ZMIZ2/ZMIP7 which both enhance its transactivation activity. Interacts with SLC30A9 and RAD54L2/ARIP4. Interacts via the ligand-binding domain with LXXLL and FXXLF motifs from NCOA1, NCOA2, NCOA3, NCOA4 and MAGEA11. The AR N-terminal poly-Gln region binds Ran resulting in enhancement of AR-mediated transactivation. Ran-binding decreases as the poly-Gln length increases. Interacts with HIP1 (via coiled coil domain). Interacts (via ligand-binding domain) with TRIM68. Interacts with TNK2. Interacts with USP26. Interacts with RNF6. Interacts (regulated by RNF6 probably through polyubiquitination) with RNF14; regulates AR transcriptional activity. Interacts with PRMT2 and TRIM24. Interacts with RACK1. Interacts with RANBP10; this interaction enhances dihydrotestosterone-induced AR transcriptional activity. Interacts with PRPF6 in a hormone-independent way; this interaction enhances dihydrotestosterone-induced AR transcriptional activity. Interacts with STK4/MST1. Interacts with ZIPK/DAPK3. Interacts with LPXN. Interacts with MAK. Part of a complex containing AR, MAK and NCOA3. Interacts with CRY1. Interacts with CCAR1 and GATA2 (By similarity). Interacts with BUD31 (By similarity).By similarity7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei703Interaction with coactivator LXXL and FXXFY motifsBy similarity1
Sitei880Interaction with coactivator FXXLF and FXXFY motifsBy similarity1

GO - Molecular functioni

  • androgen receptor binding Source: RGD
  • beta-catenin binding Source: UniProtKB
  • protein domain specific binding Source: RGD
  • signaling receptor binding Source: UniProtKB
  • transcription factor binding Source: RGD
View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi246396, 3 interactors
DIPiDIP-5963N
IntActiP15207, 1 interactor
STRINGi10116.ENSRNOP00000009129

Chemistry databases

BindingDBiP15207

Structurei

Secondary structure

1902
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi543 – 545Combined sources3
Beta strandi551 – 553Combined sources3
Beta strandi556 – 558Combined sources3
Helixi560 – 570Combined sources11
Beta strandi579 – 582Combined sources4
Turni588 – 593Combined sources6
Helixi595 – 605Combined sources11
Helixi655 – 663Combined sources9
Beta strandi674 – 676Combined sources3
Helixi680 – 703Combined sources24
Helixi708 – 710Combined sources3
Helixi713 – 740Combined sources28
Beta strandi743 – 748Combined sources6
Beta strandi751 – 753Combined sources3
Helixi755 – 760Combined sources6
Helixi764 – 779Combined sources16
Helixi784 – 795Combined sources12
Beta strandi797 – 800Combined sources4
Helixi807 – 826Combined sources20
Beta strandi827 – 829Combined sources3
Helixi833 – 866Combined sources34
Helixi867 – 869Combined sources3
Helixi876 – 884Combined sources9
Helixi886 – 890Combined sources5
Beta strandi893 – 896Combined sources4

3D structure databases

ProteinModelPortaliP15207
SMRiP15207
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15207

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini651 – 882NR LBDPROSITE-ProRule annotationAdd BLAST232

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 569Interaction with ZNF318By similarityAdd BLAST569
Regioni1 – 540ModulatingAdd BLAST540
Regioni534 – 901Interaction with LPXNBy similarityAdd BLAST368
Regioni554 – 644Interaction with HIPK31 PublicationAdd BLAST91
Regioni574 – 901Interaction with CCAR1By similarityAdd BLAST328
Regioni607 – 901Interaction with KAT7By similarityAdd BLAST295

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi63 – 67Poly-Arg5
Compositional biasi174 – 195Poly-GlnAdd BLAST22
Compositional biasi370 – 376Poly-Pro7
Compositional biasi394 – 400Poly-Ala7
Compositional biasi444 – 450Poly-Gly7

Domaini

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain (By similarity).By similarity

Sequence similaritiesi

Belongs to the nuclear hormone receptor family. NR3 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri542 – 562NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri578 – 602NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575 Eukaryota
ENOG410XRZC LUCA
HOGENOMiHOG000254783
HOVERGENiHBG007583
InParanoidiP15207
KOiK08557
PhylomeDBiP15207
TreeFamiTF350286

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR001103 Andrgn_rcpt
IPR035500 NHR_like_dom_sf
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF02166 Androgen_recep, 1 hit
PF00104 Hormone_recep, 1 hit
PF00105 zf-C4, 1 hit
PRINTSiPR00521 ANDROGENR
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 1 hit
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P15207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVQLGLGRV YPRPPSKTYR GAFQNLFQSV REAIQNPGPR HPEAASIAPP 
        60         70         80         90        100
GACLQQRQET SPRRRRRQQH PEDGSPQAHI RGTTGYLALE EEQQPSQQQS 
       110        120        130        140        150
ASEGHPESGC LPEPGAATAP GKGLPQQPPA PPDQDDSAAP STLSLLGPTF 
       160        170        180        190        200
PGLSSCSADI KDILSEAGTM QLLQQQQQQQ QQQQQQQQQQ QQQQQEVISE 
       210        220        230        240        250
GSSSVRAREA TGAPSSSKDS YLGGNSTISD SAKELCKAVS VSMGLGVEAL 
       260        270        280        290        300
EHLSPGEQLR GDCMYASLLG GPPAVRPTPC APLAECKGLS LDEGPGKGTE 
       310        320        330        340        350
ETAEYSSFKG GYAKGLEGES LGCSGSSEAG SSGTLEIPSS LSLYKSGAVD 
       360        370        380        390        400
EAAAYQNRDY YNFPLALSGP PHPPPPTHPH ARIKLENPSD YGSAWAAAAA 
       410        420        430        440        450
QCRYGDLASL HGGSVAGPST GSPPATASSS WHTLFTAEEG QLYGPGGGGG 
       460        470        480        490        500
SSSPSDAGPV APYGYTRPPQ GLASQEGDFS ASEVWYPGGV VNRVPYPSPS 
       510        520        530        540        550
CVKSEMGPWM ENYSGPYGDM RLDSTRDHVL PIDYYFPPQK TCLICGDEAS 
       560        570        580        590        600
GCHYGALTCG SCKVFFKRAA EGKQKYLCAS RNDCTIDKFR RKNCPSCRLR 
       610        620        630        640        650
KCYEAGMTLG ARKLKKLGNL KLQEEGENSS AGSPTEDPSQ KMTVSHIEGY 
       660        670        680        690        700
ECQPIFLNVL EAIEPGVVCA GHDNNQPDSF AALLSSLNEL GERQLVHVVK 
       710        720        730        740        750
WAKALPGFRN LHVDDQMAVI QYSWMGLMVF AMGWRSFTNV NSRMLYFAPD 
       760        770        780        790        800
LVFNEYRMHK SRMYSQCVRM RHLSQEFGWL QITPQEFLCM KALLLFSIIP 
       810        820        830        840        850
VDGLKNQKFF DELRMNYIKE LDRIIACKRK NPTSCSRRFY QLTKLLDSVQ 
       860        870        880        890        900
PIARELHQFT FDLLIKSHMV SVDFPEMMAE IISVQVPKIL SGKVKPIYFH 

TQ
Length:902
Mass (Da):98,218
Last modified:April 1, 1990 - v1
Checksum:i43F4064759FDCBED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195Missing in AAA40734 (PubMed:2341409).Curated1
Sequence conflicti389S → L in AAA40759 (PubMed:3174628).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti735R → Q in TFM; has only 10-15% of the androgen-binding capacity of wild-type AR. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20133 mRNA Translation: AAA40733.1
M23264 mRNA Translation: AAA40759.1
J05454 Genomic DNA Translation: AAA40734.1
PIRiB40494
RefSeqiNP_036634.1, NM_012502.1
UniGeneiRn.9813

Genome annotation databases

GeneIDi24208
KEGGirno:24208

Similar proteinsi

Entry informationi

Entry nameiANDR_RAT
AccessioniPrimary (citable) accession number: P15207
Secondary accession number(s): Q63049
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 20, 2018
This is version 196 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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