UniProtKB - P15178 (SYDC_RAT)
Protein
Aspartate--tRNA ligase, cytoplasmic
Gene
Dars1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.1 Publication
Catalytic activityi
- EC:6.1.1.121 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 229 | AspartateBy similarity | 1 | |
Binding sitei | 273 | AspartateBy similarity | 1 | |
Binding sitei | 424 | ATPBy similarity | 1 | |
Binding sitei | 427 | AspartateBy similarity | 1 | |
Binding sitei | 431 | AspartateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 273 – 275 | ATPBy similarity | 3 | |
Nucleotide bindingi | 281 – 283 | ATPBy similarity | 3 | |
Nucleotide bindingi | 472 – 475 | ATPBy similarity | 4 |
GO - Molecular functioni
- aspartate-tRNA ligase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- RNA binding Source: GO_Central
GO - Biological processi
- aspartyl-tRNA aminoacylation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate--tRNA ligase, cytoplasmic (EC:6.1.1.121 Publication)Alternative name(s): Aspartyl-tRNA synthetase Short name: AspRS |
Gene namesi | Name:Dars1 Synonyms:Dars, Drs1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621167, Dars |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: GO_Central
Other locations
- aminoacyl-tRNA synthetase multienzyme complex Source: UniProtKB
- cytoplasm Source: RGD
- synapse Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000111012 | 1 – 501 | Aspartate--tRNA ligase, cytoplasmicAdd BLAST | 501 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 74 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 249 | PhosphoserineBy similarity | 1 | |
Modified residuei | 374 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P15178 |
PaxDbi | P15178 |
PRIDEi | P15178 |
PTM databases
iPTMneti | P15178 |
PhosphoSitePlusi | P15178 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000003743, Expressed in quadriceps femoris and 21 other tissues |
ExpressionAtlasi | P15178, baseline and differential |
Genevisiblei | P15178, RN |
Interactioni
Subunit structurei
Homodimer (PubMed:2642907).
Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 250460, 5 interactors |
IntActi | P15178, 3 interactors |
STRINGi | 10116.ENSRNOP00000005127 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 251 – 254 | AspartateBy similarity | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0556, Eukaryota |
GeneTreei | ENSGT01010000222484 |
HOGENOMi | CLU_004553_2_1_1 |
InParanoidi | P15178 |
OMAi | WVHEIRD |
OrthoDBi | 677307at2759 |
PhylomeDBi | P15178 |
TreeFami | TF105676 |
Family and domain databases
HAMAPi | MF_02075, Asp_tRNA_synth_type2, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004523, Asp-tRNA_synthase_2 IPR002312, Asp/Asn-tRNA-synth_IIb IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR43450, PTHR43450, 1 hit |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00458, aspS_nondisc, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P15178-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSANASRKG QEKPREIVDA AEDYAKERYG VSSMIQSQEK PDRVLVRVKD
60 70 80 90 100
LTVQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ
110 120 130 140 150
MVKFAANINK ESIIDVEGIV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP
160 170 180 190 200
RLPLQLDDAI RPEVEGEEDG RATVNQDTRL DNRIIDLRTS TSQAIFHLQS
210 220 230 240 250
GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KSNAYLAQSP
260 270 280 290 300
QLYKQMCICA DFEKVFCIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH
310 320 330 340 350
EVVEEIADTL VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE
360 370 380 390 400
ALAMLREAGV EMDDEEDLST PNEKLLGRLV KEKYDTDFYV LDKYPLAVRP
410 420 430 440 450
FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE
460 470 480 490 500
KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT
P
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04487 mRNA Translation: AAA40789.1 U30812 , U30485, U30800, U30801, U30802, U30803, U30804, U30805, U30806, U30807, U30808, U30809, U30810, U30811 Genomic DNA Translation: AAC52981.1 BC072534 mRNA Translation: AAH72534.1 |
PIRi | A32197, SYRTDT |
RefSeqi | NP_446251.1, NM_053799.3 |
Genome annotation databases
Ensembli | ENSRNOT00000005127; ENSRNOP00000005127; ENSRNOG00000003743 |
GeneIDi | 116483 |
KEGGi | rno:116483 |
UCSCi | RGD:621167, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04487 mRNA Translation: AAA40789.1 U30812 , U30485, U30800, U30801, U30802, U30803, U30804, U30805, U30806, U30807, U30808, U30809, U30810, U30811 Genomic DNA Translation: AAC52981.1 BC072534 mRNA Translation: AAH72534.1 |
PIRi | A32197, SYRTDT |
RefSeqi | NP_446251.1, NM_053799.3 |
3D structure databases
SMRi | P15178 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250460, 5 interactors |
IntActi | P15178, 3 interactors |
STRINGi | 10116.ENSRNOP00000005127 |
PTM databases
iPTMneti | P15178 |
PhosphoSitePlusi | P15178 |
Proteomic databases
jPOSTi | P15178 |
PaxDbi | P15178 |
PRIDEi | P15178 |
Genome annotation databases
Ensembli | ENSRNOT00000005127; ENSRNOP00000005127; ENSRNOG00000003743 |
GeneIDi | 116483 |
KEGGi | rno:116483 |
UCSCi | RGD:621167, rat |
Organism-specific databases
CTDi | 226414 |
RGDi | 621167, Dars |
Phylogenomic databases
eggNOGi | KOG0556, Eukaryota |
GeneTreei | ENSGT01010000222484 |
HOGENOMi | CLU_004553_2_1_1 |
InParanoidi | P15178 |
OMAi | WVHEIRD |
OrthoDBi | 677307at2759 |
PhylomeDBi | P15178 |
TreeFami | TF105676 |
Miscellaneous databases
PROi | PR:P15178 |
Gene expression databases
Bgeei | ENSRNOG00000003743, Expressed in quadriceps femoris and 21 other tissues |
ExpressionAtlasi | P15178, baseline and differential |
Genevisiblei | P15178, RN |
Family and domain databases
HAMAPi | MF_02075, Asp_tRNA_synth_type2, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004523, Asp-tRNA_synthase_2 IPR002312, Asp/Asn-tRNA-synth_IIb IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR43450, PTHR43450, 1 hit |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00458, aspS_nondisc, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYDC_RAT | |
Accessioni | P15178Primary (citable) accession number: P15178 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries