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Protein

Myoblast determination protein 1

Gene

MYOD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Myogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-375170 CDO in myogenesis
SignaLinkiP15172
SIGNORiP15172

Names & Taxonomyi

Protein namesi
Recommended name:
Myoblast determination protein 1
Alternative name(s):
Class C basic helix-loop-helix protein 1
Short name:
bHLHc1
Myogenic factor 3
Short name:
Myf-3
Gene namesi
Name:MYOD1
Synonyms:BHLHC1, MYF3, MYOD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000129152.3
HGNCiHGNC:7611 MYOD1
MIMi159970 gene
neXtProtiNX_P15172

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4654
OpenTargetsiENSG00000129152
PharmGKBiPA31416

Polymorphism and mutation databases

BioMutaiMYOD1
DMDMi209572729

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273601 – 320Myoblast determination protein 1Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki1Peptide (Met-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei104N6-methyllysine; by EHMT21 Publication1

Post-translational modificationi

Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.1 Publication
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).By similarity
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.1 Publication
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP15172
PeptideAtlasiP15172
PRIDEiP15172
ProteomicsDBi53118

PTM databases

iPTMnetiP15172
PhosphoSitePlusiP15172

Expressioni

Gene expression databases

BgeeiENSG00000129152 Expressed in 33 organ(s), highest expression level in skeletal muscle tissue
CleanExiHS_MYOD1
GenevisibleiP15172 HS

Organism-specific databases

HPAiHPA051362

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 (By similarity). Interacts with SETD3 (By similarity). Interacts with P-TEFB complex; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (By similarity) (PubMed:12037670). Interacts with CSRP3.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TCF3P15923-12EBI-488878,EBI-769645

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110737, 69 interactors
CORUMiP15172
DIPiDIP-704N
IntActiP15172, 16 interactors
MINTiP15172
STRINGi9606.ENSP00000250003

Structurei

3D structure databases

ProteinModelPortaliP15172
SMRiP15172
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 160bHLHPROSITE-ProRule annotationAdd BLAST52

Phylogenomic databases

eggNOGiKOG3960 Eukaryota
ENOG4111SED LUCA
GeneTreeiENSGT00530000063004
HOGENOMiHOG000234800
HOVERGENiHBG006429
InParanoidiP15172
KOiK09064
OMAiPNPIYQV
OrthoDBiEOG091G0GKD
PhylomeDBiP15172
TreeFamiTF316344

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR002546 Basic
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR022032 Myf5
PfamiView protein in Pfam
PF01586 Basic, 1 hit
PF00010 HLH, 1 hit
PF12232 Myf5, 1 hit
SMARTiView protein in SMART
SM00520 BASIC, 1 hit
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

P15172-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELLSPPLRD VDLTAPDGSL CSFATTDDFY DDPCFDSPDL RFFEDLDPRL
60 70 80 90 100
MHVGALLKPE EHSHFPAAVH PAPGAREDEH VRAPSGHHQA GRCLLWACKA
110 120 130 140 150
CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL
160 170 180 190 200
RNAIRYIEGL QALLRDQDAA PPGAAAAFYA PGPLPPGRGG EHYSGDSDAS
210 220 230 240 250
SPRSNCSDGM MDYSGPPSGA RRRNCYEGAY YNEAPSEPRP GKSAAVSSLD
260 270 280 290 300
CLSSIVERIS TESPAAPALL LADVPSESPP RRQEAAAPSE GESSGDPTQS
310 320
PDAAPQCPAG ANPNPIYQVL
Length:320
Mass (Da):34,501
Last modified:October 14, 2008 - v3
Checksum:i75E624D2ED5B0B33
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124K → E in CAA35640 (PubMed:2583111).Curated1
Sequence conflicti177Missing in CAA40000 (PubMed:1850513).Curated1
Sequence conflicti177Missing in CAA35640 (PubMed:2583111).Curated1
Sequence conflicti251C → Y in CAA40000 (PubMed:1850513).Curated1
Sequence conflicti251C → Y in CAA35640 (PubMed:2583111).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036392262E → K in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs930939009Ensembl.1
Natural variantiVAR_036393309A → V in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56677 mRNA Translation: CAA40000.1
AF027148 Genomic DNA Translation: AAC29001.1
BT007157 mRNA Translation: AAP35821.1
CH471064 Genomic DNA Translation: EAW68427.1
BC064493 mRNA Translation: AAH64493.1
X17650 mRNA Translation: CAA35640.1
CCDSiCCDS7826.1
PIRiS26827
RefSeqiNP_002469.2, NM_002478.4
UniGeneiHs.181768

Genome annotation databases

EnsembliENST00000250003; ENSP00000250003; ENSG00000129152
GeneIDi4654
KEGGihsa:4654
UCSCiuc001mni.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

MyoD entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56677 mRNA Translation: CAA40000.1
AF027148 Genomic DNA Translation: AAC29001.1
BT007157 mRNA Translation: AAP35821.1
CH471064 Genomic DNA Translation: EAW68427.1
BC064493 mRNA Translation: AAH64493.1
X17650 mRNA Translation: CAA35640.1
CCDSiCCDS7826.1
PIRiS26827
RefSeqiNP_002469.2, NM_002478.4
UniGeneiHs.181768

3D structure databases

ProteinModelPortaliP15172
SMRiP15172
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110737, 69 interactors
CORUMiP15172
DIPiDIP-704N
IntActiP15172, 16 interactors
MINTiP15172
STRINGi9606.ENSP00000250003

PTM databases

iPTMnetiP15172
PhosphoSitePlusiP15172

Polymorphism and mutation databases

BioMutaiMYOD1
DMDMi209572729

Proteomic databases

PaxDbiP15172
PeptideAtlasiP15172
PRIDEiP15172
ProteomicsDBi53118

Protocols and materials databases

DNASUi4654
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000250003; ENSP00000250003; ENSG00000129152
GeneIDi4654
KEGGihsa:4654
UCSCiuc001mni.4 human

Organism-specific databases

CTDi4654
DisGeNETi4654
EuPathDBiHostDB:ENSG00000129152.3
GeneCardsiMYOD1
H-InvDBiHIX0035989
HGNCiHGNC:7611 MYOD1
HPAiHPA051362
MIMi159970 gene
neXtProtiNX_P15172
OpenTargetsiENSG00000129152
PharmGKBiPA31416
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3960 Eukaryota
ENOG4111SED LUCA
GeneTreeiENSGT00530000063004
HOGENOMiHOG000234800
HOVERGENiHBG006429
InParanoidiP15172
KOiK09064
OMAiPNPIYQV
OrthoDBiEOG091G0GKD
PhylomeDBiP15172
TreeFamiTF316344

Enzyme and pathway databases

ReactomeiR-HSA-375170 CDO in myogenesis
SignaLinkiP15172
SIGNORiP15172

Miscellaneous databases

ChiTaRSiMYOD1 human
GeneWikiiMyoD
GenomeRNAii4654
PROiPR:P15172
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000129152 Expressed in 33 organ(s), highest expression level in skeletal muscle tissue
CleanExiHS_MYOD1
GenevisibleiP15172 HS

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR002546 Basic
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR022032 Myf5
PfamiView protein in Pfam
PF01586 Basic, 1 hit
PF00010 HLH, 1 hit
PF12232 Myf5, 1 hit
SMARTiView protein in SMART
SM00520 BASIC, 1 hit
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMYOD1_HUMAN
AccessioniPrimary (citable) accession number: P15172
Secondary accession number(s): O75321
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 14, 2008
Last modified: September 12, 2018
This is version 189 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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