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Protein

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Gene

GSPT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

Caution

eRF3 antibodies used in PubMed:19417104 do not differentiate between GSPT1/ERF3A and GSPT2/ERF3B.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 88GTPBy similarity8
Nucleotide bindingi158 – 162GTPBy similarity5
Nucleotide bindingi220 – 223GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB
  • translation release factor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processNonsense-mediated mRNA decay, Protein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Short name:
Eukaryotic peptide chain release factor subunit 3a
Short name:
eRF3a
Alternative name(s):
G1 to S phase transition protein 1 homolog
Gene namesi
Name:GSPT1
Synonyms:ERF3A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103342.12
HGNCiHGNC:4621 GSPT1
MIMi139259 gene
neXtProtiNX_P15170

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi2935
OpenTargetsiENSG00000103342
PharmGKBiPA29012

Chemistry databases

DrugBankiDB04315 Guanosine-5'-Diphosphate

Polymorphism and mutation databases

BioMutaiGSPT1
DMDMi121688

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914801 – 499Eukaryotic peptide chain release factor GTP-binding subunit ERF3AAdd BLAST499

Proteomic databases

EPDiP15170
MaxQBiP15170
PaxDbiP15170
PeptideAtlasiP15170
PRIDEiP15170
ProteomicsDBi53116
53117 [P15170-2]

PTM databases

iPTMnetiP15170
PhosphoSitePlusiP15170
SwissPalmiP15170

Miscellaneous databases

PMAP-CutDBiP15170

Expressioni

Gene expression databases

BgeeiENSG00000103342 Expressed in 232 organ(s), highest expression level in amniotic fluid
CleanExiHS_GSPT1
ExpressionAtlasiP15170 baseline and differential
GenevisibleiP15170 HS

Organism-specific databases

HPAiHPA052488

Interactioni

Subunit structurei

Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi109190, 41 interactors
ComplexPortaliCPX-2721 Translation release factor ERF1-ERF3 complex
CORUMiP15170
IntActiP15170, 10 interactors
MINTiP15170
STRINGi9606.ENSP00000398131

Structurei

3D structure databases

ProteinModelPortaliP15170
SMRiP15170
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15170

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 298tr-type GPROSITE-ProRule annotationAdd BLAST227

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 88G1PROSITE-ProRule annotation8
Regioni137 – 141G2PROSITE-ProRule annotation5
Regioni158 – 161G3PROSITE-ProRule annotation4
Regioni220 – 223G4PROSITE-ProRule annotation4
Regioni262 – 264G5PROSITE-ProRule annotation3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0459 Eukaryota
COG5256 LUCA
GeneTreeiENSGT00620000087924
HOGENOMiHOG000229291
HOVERGENiHBG000179
InParanoidiP15170
KOiK03267
OMAiIDVAHIY
OrthoDBiEOG091G0637
PhylomeDBiP15170
TreeFamiTF300566

Family and domain databases

InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P15170-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELSEPIVEN GETEMSPEES WEHKEEISEA EPGGGSLGDG RPPEESAHEM
60 70 80 90 100
MEEEEEIPKP KSVVAPPGAP KKEHVNVVFI GHVDAGKSTI GGQIMYLTGM
110 120 130 140 150
VDKRTLEKYE REAKEKNRET WYLSWALDTN QEERDKGKTV EVGRAYFETE
160 170 180 190 200
KKHFTILDAP GHKSFVPNMI GGASQADLAV LVISARKGEF ETGFEKGGQT
210 220 230 240 250
REHAMLAKTA GVKHLIVLIN KMDDPTVNWS NERYEECKEK LVPFLKKVGF
260 270 280 290 300
NPKKDIHFMP CSGLTGANLK EQSDFCPWYI GLPFIPYLDN LPNFNRSVDG
310 320 330 340 350
PIRLPIVDKY KDMGTVVLGK LESGSICKGQ QLVMMPNKHN VEVLGILSDD
360 370 380 390 400
VETDTVAPGE NLKIRLKGIE EEEILPGFIL CDPNNLCHSG RTFDAQIVII
410 420 430 440 450
EHKSIICPGY NAVLHIHTCI EEVEITALIC LVDKKSGEKS KTRPRFVKQD
460 470 480 490
QVCIARLRTA GTICLETFKD FPQMGRFTLR DEGKTIAIGK VLKLVPEKD
Length:499
Mass (Da):55,756
Last modified:April 1, 1990 - v1
Checksum:iDE20482CCABC3576
GO
Isoform 2 (identifier: P15170-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MDPGSGGGGG...CEGSNSAVSM
     8-8: Missing.

Show »
Length:636
Mass (Da):68,601
Checksum:i31E3FE3DEDF4BE5E
GO
Isoform 3 (identifier: P15170-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MDPGSGGGGG...CEGSNSAVSM

Note: Gene prediction based on EST data.
Show »
Length:637
Mass (Da):68,700
Checksum:i7765D73A284F35C4
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BSV8H3BSV8_HUMAN
Eukaryotic peptide chain release fa...
GSPT1
199Annotation score:
H3BR35H3BR35_HUMAN
Eukaryotic peptide chain release fa...
GSPT1
475Annotation score:
H3BMT9H3BMT9_HUMAN
Eukaryotic peptide chain release fa...
GSPT1
94Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: P15170-2)
Sequence conflicti6 – 9GGGG → G in AAH09503 (PubMed:15489334).Curated4
Sequence conflicti92G → C in AAH09503 (PubMed:15489334).Curated1
Sequence conflicti100V → A in AAH09503 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0421981M → MDPGSGGGGGGGGGGGSSSG SSSSDSAPDCWDQADMEAPG PGPCGGGGSLAAAAEAQREN LSAAFSRQLNVNAKPFVPNV HAAEFVPSFLRGPAAPPPPV GGAANNHGAGSGAGGRAAPV ESSQEEQSLCEGSNSAVSM in isoform 2 and isoform 3. 1 Publication1
Alternative sequenceiVSP_0421998Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17644 mRNA Translation: CAA35635.1
U95742 Genomic DNA Translation: AAB67250.1
AC007216 Genomic DNA No translation available.
BC009503 mRNA Translation: AAH09503.2
CCDSiCCDS45412.1 [P15170-3]
CCDS45413.1 [P15170-2]
CCDS45414.1 [P15170-1]
PIRiS06941
RefSeqiNP_001123478.1, NM_001130006.1
NP_001123479.1, NM_001130007.1 [P15170-1]
UniGeneiHs.528780

Genome annotation databases

EnsembliENST00000420576; ENSP00000399539; ENSG00000103342 [P15170-1]
ENST00000434724; ENSP00000398131; ENSG00000103342 [P15170-3]
ENST00000439887; ENSP00000408399; ENSG00000103342 [P15170-2]
ENST00000563468; ENSP00000454351; ENSG00000103342 [P15170-1]
GeneIDi2935
KEGGihsa:2935
UCSCiuc002dbt.4 human [P15170-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17644 mRNA Translation: CAA35635.1
U95742 Genomic DNA Translation: AAB67250.1
AC007216 Genomic DNA No translation available.
BC009503 mRNA Translation: AAH09503.2
CCDSiCCDS45412.1 [P15170-3]
CCDS45413.1 [P15170-2]
CCDS45414.1 [P15170-1]
PIRiS06941
RefSeqiNP_001123478.1, NM_001130006.1
NP_001123479.1, NM_001130007.1 [P15170-1]
UniGeneiHs.528780

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3E1YX-ray3.80E/F/G/H301-499[»]
3J5Yelectron microscopy9.70B69-496[»]
3KUIX-ray2.30B64-78[»]
4D61electron microscopy9.00i72-497[»]
5HXBX-ray3.60A/X300-496[»]
5LZTelectron microscopy3.65jj1-499[»]
ProteinModelPortaliP15170
SMRiP15170
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109190, 41 interactors
ComplexPortaliCPX-2721 Translation release factor ERF1-ERF3 complex
CORUMiP15170
IntActiP15170, 10 interactors
MINTiP15170
STRINGi9606.ENSP00000398131

Chemistry databases

DrugBankiDB04315 Guanosine-5'-Diphosphate

PTM databases

iPTMnetiP15170
PhosphoSitePlusiP15170
SwissPalmiP15170

Polymorphism and mutation databases

BioMutaiGSPT1
DMDMi121688

Proteomic databases

EPDiP15170
MaxQBiP15170
PaxDbiP15170
PeptideAtlasiP15170
PRIDEiP15170
ProteomicsDBi53116
53117 [P15170-2]

Protocols and materials databases

DNASUi2935
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000420576; ENSP00000399539; ENSG00000103342 [P15170-1]
ENST00000434724; ENSP00000398131; ENSG00000103342 [P15170-3]
ENST00000439887; ENSP00000408399; ENSG00000103342 [P15170-2]
ENST00000563468; ENSP00000454351; ENSG00000103342 [P15170-1]
GeneIDi2935
KEGGihsa:2935
UCSCiuc002dbt.4 human [P15170-1]

Organism-specific databases

CTDi2935
DisGeNETi2935
EuPathDBiHostDB:ENSG00000103342.12
GeneCardsiGSPT1
HGNCiHGNC:4621 GSPT1
HPAiHPA052488
MIMi139259 gene
neXtProtiNX_P15170
OpenTargetsiENSG00000103342
PharmGKBiPA29012
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0459 Eukaryota
COG5256 LUCA
GeneTreeiENSGT00620000087924
HOGENOMiHOG000229291
HOVERGENiHBG000179
InParanoidiP15170
KOiK03267
OMAiIDVAHIY
OrthoDBiEOG091G0637
PhylomeDBiP15170
TreeFamiTF300566

Enzyme and pathway databases

ReactomeiR-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

ChiTaRSiGSPT1 human
EvolutionaryTraceiP15170
GeneWikiiGSPT1
GenomeRNAii2935
PMAP-CutDBiP15170
PROiPR:P15170
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103342 Expressed in 232 organ(s), highest expression level in amniotic fluid
CleanExiHS_GSPT1
ExpressionAtlasiP15170 baseline and differential
GenevisibleiP15170 HS

Family and domain databases

InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiERF3A_HUMAN
AccessioniPrimary (citable) accession number: P15170
Secondary accession number(s): J3KQG6, Q96GF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 12, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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