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Protein

Ras-related C3 botulinum toxin substrate 2

Gene

RAC2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.1 Publication

Activity regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi115 – 118GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB
  • protein kinase regulator activity Source: Ensembl

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-114604 GPVI-mediated activation cascade
R-HSA-194840 Rho GTPase cycle
R-HSA-4086400 PCP/CE pathway
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-5668599 RHO GTPases Activate NADPH Oxidases
SignaLinkiP15153
SIGNORiP15153

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related C3 botulinum toxin substrate 2
Alternative name(s):
GX
Small G protein
p21-Rac2
Gene namesi
Name:RAC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000128340.14
HGNCiHGNC:9802 RAC2
MIMi602049 gene
neXtProtiNX_P15153

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Neutrophil immunodeficiency syndrome (NEUID)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn immunodeficiency syndrome due to defective neutrophils. Affected individuals present with leukocytosis, neutrophilia, severe recurrent bacterial infections and poor wound healing.
See also OMIM:608203
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01745257D → N in NEUID; dominant-negative mutant; binds GDP, but not GTP; inhibits oxidase activation and superoxide anion production in vitro. 1 PublicationCorresponds to variant dbSNP:rs74315507EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189C → W: Abolishes in vitro prenylation. 1 Publication1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi5880
MalaCardsiRAC2
MIMi608203 phenotype
OpenTargetsiENSG00000128340
Orphaneti183707 Neutrophil immunodeficiency syndrome
PharmGKBiPA34163

Chemistry databases

ChEMBLiCHEMBL5581
DrugBankiDB00514 Dextromethorphan

Polymorphism and mutation databases

DMDMi131806

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000420461 – 189Ras-related C3 botulinum toxin substrate 2Add BLAST189
PropeptideiPRO_0000042047190 – 192Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_022301 Publication1
Modified residuei39ADP-ribosylasparagine; by botulinum toxinBy similarity1
Modified residuei147N6-acetyllysineCombined sources1
Modified residuei189Cysteine methyl esterBy similarity1
Lipidationi189S-geranylgeranyl cysteine1 Publication1

Post-translational modificationi

(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.1 Publication

Keywords - PTMi

Acetylation, ADP-ribosylation, Glycoprotein, Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP15153
MaxQBiP15153
PaxDbiP15153
PeptideAtlasiP15153
PRIDEiP15153
ProteomicsDBi53114

PTM databases

iPTMnetiP15153
PhosphoSitePlusiP15153
SwissPalmiP15153

Expressioni

Tissue specificityi

Hematopoietic specific.

Gene expression databases

BgeeiENSG00000128340 Expressed in 201 organ(s), highest expression level in blood
CleanExiHS_RAC2
ExpressionAtlasiP15153 baseline and differential
GenevisibleiP15153 HS

Organism-specific databases

HPAiCAB022946
HPA047820

Interactioni

Subunit structurei

Interacts with DOCK2, which may activate it. Interacts with S100A8 and calprotectin (S100A8/9) (PubMed:10559471, PubMed:15642721). Found in a complex with SH3RF1, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8IP1/JIP1, MAPK8/JNK1 and MAPK9/JNK2 (By similarity).By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi111818, 25 interactors
DIPiDIP-34291N
IntActiP15153, 17 interactors
MINTiP15153
STRINGi9606.ENSP00000249071

Structurei

Secondary structure

1192
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP15153
SMRiP15153
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15153

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiP15153
KOiK07860
OMAiLCPQPAK
OrthoDBiEOG091G0KCM
PhylomeDBiP15153
TreeFamiTF101109

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P15153-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDSKP
60 70 80 90 100
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASY ENVRAKWFPE
110 120 130 140 150
VRHHCPSTPI ILVGTKLDLR DDKDTIEKLK EKKLAPITYP QGLALAKEID
160 170 180 190
SVKYLECSAL TQRGLKTVFD EAIRAVLCPQ PTRQQKRACS LL
Length:192
Mass (Da):21,429
Last modified:April 1, 1990 - v1
Checksum:i2A1F1266B07C3210
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AH80B1AH80_HUMAN
Ras-related C3 botulinum toxin subs...
RAC2 hCG_1811995
185Annotation score:
B1AH77B1AH77_HUMAN
Ras-related C3 botulinum toxin subs...
RAC2
148Annotation score:
B1AH78B1AH78_HUMAN
Ras-related C3 botulinum toxin subs...
RAC2
166Annotation score:
B1AH79B1AH79_HUMAN
Ras-related C3 botulinum toxin subs...
RAC2 hCG_1811995
86Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03656929P → L in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_01745257D → N in NEUID; dominant-negative mutant; binds GDP, but not GTP; inhibits oxidase activation and superoxide anion production in vitro. 1 PublicationCorresponds to variant dbSNP:rs74315507EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29871 mRNA Translation: AAA36538.1
AF498965 mRNA Translation: AAM21112.1
CR456555 mRNA Translation: CAG30441.1
BT006919 mRNA Translation: AAP35565.1
Z82188 Genomic DNA No translation available.
BC001485 mRNA Translation: AAH01485.1
M64595 mRNA Translation: AAA35941.1
CCDSiCCDS13945.1
PIRiB34386
RefSeqiNP_002863.1, NM_002872.4
UniGeneiHs.517601

Genome annotation databases

EnsembliENST00000249071; ENSP00000249071; ENSG00000128340
GeneIDi5880
KEGGihsa:5880
UCSCiuc003arc.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
RAC2base

RAC2 mutation db

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29871 mRNA Translation: AAA36538.1
AF498965 mRNA Translation: AAM21112.1
CR456555 mRNA Translation: CAG30441.1
BT006919 mRNA Translation: AAP35565.1
Z82188 Genomic DNA No translation available.
BC001485 mRNA Translation: AAH01485.1
M64595 mRNA Translation: AAA35941.1
CCDSiCCDS13945.1
PIRiB34386
RefSeqiNP_002863.1, NM_002872.4
UniGeneiHs.517601

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DS6X-ray2.35A1-192[»]
2W2TX-ray1.95A2-179[»]
2W2VX-ray2.00A/B/C/D1-177[»]
2W2XX-ray2.30A/B2-179[»]
ProteinModelPortaliP15153
SMRiP15153
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111818, 25 interactors
DIPiDIP-34291N
IntActiP15153, 17 interactors
MINTiP15153
STRINGi9606.ENSP00000249071

Chemistry databases

ChEMBLiCHEMBL5581
DrugBankiDB00514 Dextromethorphan

PTM databases

iPTMnetiP15153
PhosphoSitePlusiP15153
SwissPalmiP15153

Polymorphism and mutation databases

DMDMi131806

Proteomic databases

EPDiP15153
MaxQBiP15153
PaxDbiP15153
PeptideAtlasiP15153
PRIDEiP15153
ProteomicsDBi53114

Protocols and materials databases

DNASUi5880
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249071; ENSP00000249071; ENSG00000128340
GeneIDi5880
KEGGihsa:5880
UCSCiuc003arc.5 human

Organism-specific databases

CTDi5880
DisGeNETi5880
EuPathDBiHostDB:ENSG00000128340.14
GeneCardsiRAC2
HGNCiHGNC:9802 RAC2
HPAiCAB022946
HPA047820
MalaCardsiRAC2
MIMi602049 gene
608203 phenotype
neXtProtiNX_P15153
OpenTargetsiENSG00000128340
Orphaneti183707 Neutrophil immunodeficiency syndrome
PharmGKBiPA34163
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiP15153
KOiK07860
OMAiLCPQPAK
OrthoDBiEOG091G0KCM
PhylomeDBiP15153
TreeFamiTF101109

Enzyme and pathway databases

ReactomeiR-HSA-114604 GPVI-mediated activation cascade
R-HSA-194840 Rho GTPase cycle
R-HSA-4086400 PCP/CE pathway
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-5668599 RHO GTPases Activate NADPH Oxidases
SignaLinkiP15153
SIGNORiP15153

Miscellaneous databases

ChiTaRSiRAC2 human
EvolutionaryTraceiP15153
GeneWikiiRAC2
GenomeRNAii5880
PROiPR:P15153
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000128340 Expressed in 201 organ(s), highest expression level in blood
CleanExiHS_RAC2
ExpressionAtlasiP15153 baseline and differential
GenevisibleiP15153 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAC2_HUMAN
AccessioniPrimary (citable) accession number: P15153
Secondary accession number(s): Q9UDJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 7, 2018
This is version 206 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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