UniProtKB - P15130 (NCAP_CVH22)
Protein
Nucleoprotein
Gene
N
Organism
Human coronavirus 229E (HCoV-229E)
Status
Functioni
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.UniRule annotation1 Publication
GO - Molecular functioni
- identical protein binding Source: IntAct
- RNA binding Source: UniProtKB-KW
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationAlternative name(s): Nucleocapsid proteinUniRule annotation Short name: NCUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NUniRule annotation ORF Names:7 |
Organismi | Human coronavirus 229E (HCoV-229E) |
Taxonomic identifieri | 11137 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Alphacoronavirus › Duvinacovirus |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host endoplasmic reticulum-Golgi intermediate compartment UniRule annotation
- Host Golgi apparatus UniRule annotation Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.UniRule annotation
GO - Cellular componenti
- host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
- host cell Golgi apparatus Source: UniProtKB-SubCell
- viral nucleocapsid Source: UniProtKB-KW
Keywords - Cellular componenti
Host Golgi apparatus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106000 | 1 – 389 | NucleoproteinAdd BLAST | 389 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 145 | Phosphoserine; by hostUniRule annotation | 1 |
Post-translational modificationi
ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.UniRule annotation
Phosphorylated on serine and threonine residues.UniRule annotation
Keywords - PTMi
ADP-ribosylation, PhosphoproteinProteomic databases
PRIDEi | P15130 |
Interactioni
Subunit structurei
Homooligomer. Both monomeric and oligomeric forms interact with RNA.
Interacts with protein M.
Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.
UniRule annotationBinary interactionsi
P15130
With | #Exp. | IntAct |
---|---|---|
itself | 19 | EBI-8172439,EBI-8172439 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
IntActi | P15130, 3 interactors |
MINTi | P15130 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 141 | CoV N NTDPROSITE-ProRule annotationAdd BLAST | 122 | |
Domaini | 236 – 352 | CoV N CTDPROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 148 | RNA-bindingUniRule annotationAdd BLAST | 127 | |
Regioni | 243 – 347 | DimerizationUniRule annotationAdd BLAST | 105 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 145 – 178 | Ser-richAdd BLAST | 34 |
Sequence similaritiesi
Belongs to the alphacoronavirus nucleocapsid protein family.UniRule annotation
Family and domain databases
HAMAPi | MF_04095, ALPHA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR042548, NCAP_aCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
i Sequence
Sequence statusi: Complete.
P15130-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATVKWADAS EPQRGRQGRI PYSLYSPLLV DSEQPWKVIP RNLVPINKKD
60 70 80 90 100
KNKLIGYWNV QKRFRTRKGK RVDLSPKLHF YYLGTGPHKD AKFRERVEGV
110 120 130 140 150
VWVAVDGAKT EPTGYGVRRK NSEPEIPHFN QKLPNGVTVV EEPDSRAPSR
160 170 180 190 200
SQSRSQSRGR GESKPQSRNP SSDRNHNSQD DIMKAVAAAL KSLGFDKPQE
210 220 230 240 250
KDKKSAKTGT PKPSRNQSPA SSQTSAKSLA RSQSSETKEQ KHEMQKPRWK
260 270 280 290 300
RQPNDDVTSN VTQCFGPRDL DHNFGSAGVV ANGVKAKGYP QFAELVPSTA
310 320 330 340 350
AMLFDSHIVS KESGNTVVLT FTTRVTVPKD HPHLGKFLEE LNAFTREMQQ
360 370 380
HPLLNPSALE FNPSQTSPAT AEPVRDEVSI ETDIIDEVN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 | P → S in AAA45463 (PubMed:2922924). | 1 | |
Sequence conflicti | 115 | Y → H (PubMed:2922924). | 1 | |
Sequence conflicti | 117 | V → A (PubMed:2922924). | 1 | |
Sequence conflicti | 160 | R → P in AAA45463 (PubMed:2922924). | 1 | |
Sequence conflicti | 175 | N → Y in AAA45463 (PubMed:2922924). | 1 | |
Sequence conflicti | 244 – 245 | MQ → IE in AAA45463 (PubMed:2922924). | 2 | |
Sequence conflicti | 351 | H → Q in AAA45463 (PubMed:2922924). | 1 | |
Sequence conflicti | 378 | V → F in AAA45463 (PubMed:2922924). | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04419 Genomic RNA Translation: AAA45463.1 X51325 mRNA Translation: CAA35708.1 AF304460 Genomic RNA Translation: AAG48597.1 |
PIRi | A30119, VHIH2E S08031 |
RefSeqi | NP_073556.1, NC_002645.1 |
Genome annotation databases
GeneIDi | 918763 |
KEGGi | vg:918763 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04419 Genomic RNA Translation: AAA45463.1 X51325 mRNA Translation: CAA35708.1 AF304460 Genomic RNA Translation: AAG48597.1 |
PIRi | A30119, VHIH2E S08031 |
RefSeqi | NP_073556.1, NC_002645.1 |
3D structure databases
SMRi | P15130 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P15130, 3 interactors |
MINTi | P15130 |
Proteomic databases
PRIDEi | P15130 |
Genome annotation databases
GeneIDi | 918763 |
KEGGi | vg:918763 |
Family and domain databases
HAMAPi | MF_04095, ALPHA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR042548, NCAP_aCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_CVH22 | |
Accessioni | P15130Primary (citable) accession number: P15130 Secondary accession number(s): Q66175 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 11, 2003 | |
Last modified: | October 7, 2020 | |
This is version 101 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families