UniProtKB - P15116 (CADH2_MOUSE)
Cadherin-2
Cdh2
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 170 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 170 | Calcium 2Combined sources1 Publication | 1 | |
Metal bindingi | 226 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 228 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 228 | Calcium 2Combined sources1 Publication | 1 | |
Metal bindingi | 259 | Calcium 2Combined sources1 Publication | 1 | |
Metal bindingi | 260 | Calcium 2; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 261 | Calcium 3Combined sources1 Publication | 1 | |
Metal bindingi | 262 | Calcium 1Combined sources2 Publications | 1 | |
Metal bindingi | 262 | Calcium 2Combined sources2 Publications | 1 | |
Metal bindingi | 263 | Calcium 3; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 293 | Calcium 3Combined sources2 Publications | 1 | |
Metal bindingi | 295 | Calcium 2Combined sources1 Publication | 1 | |
Metal bindingi | 301 | Calcium 3; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 353 | Calcium 3Combined sources1 Publication | 1 |
GO - Molecular functioni
- alpha-catenin binding Source: MGI
- beta-catenin binding Source: MGI
- cadherin binding Source: GO_Central
- calcium ion binding Source: UniProtKB
- enzyme binding Source: UniProtKB
- gamma-catenin binding Source: MGI
- identical protein binding Source: IntAct
- nitric-oxide synthase binding Source: MGI
- protein-containing complex binding Source: MGI
- protein kinase binding Source: UniProtKB
- protein phosphatase binding Source: UniProtKB
GO - Biological processi
- blood vessel morphogenesis Source: MGI
- brain development Source: GO_Central
- brain morphogenesis Source: MGI
- calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
- cell adhesion Source: MGI
- cell-cell adhesion Source: UniProtKB
- cell-cell adhesion mediated by cadherin Source: UniProtKB
- cell-cell adhesion via plasma-membrane adhesion molecules Source: GO_Central
- cell migration Source: MGI
- cerebral cortex development Source: MGI
- glial cell differentiation Source: UniProtKB
- heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
- homeostasis of number of cells Source: MGI
- homophilic cell adhesion via plasma membrane adhesion molecules Source: MGI
- modulation of chemical synaptic transmission Source: MGI
- negative regulation of canonical Wnt signaling pathway Source: MGI
- neuroepithelial cell differentiation Source: MGI
- neuroligin clustering involved in postsynaptic membrane assembly Source: BHF-UCL
- neuronal stem cell population maintenance Source: UniProtKB
- positive regulation of MAPK cascade Source: MGI
- positive regulation of synaptic vesicle clustering Source: BHF-UCL
- protein localization to plasma membrane Source: UniProtKB
- radial glial cell differentiation Source: MGI
- regulation of axonogenesis Source: MGI
- regulation of myelination Source: MGI
- regulation of oligodendrocyte progenitor proliferation Source: MGI
- regulation of postsynaptic density protein 95 clustering Source: BHF-UCL
- regulation of protein localization Source: MGI
- regulation of Rho protein signal transduction Source: MGI
- regulation of synaptic transmission, glutamatergic Source: MGI
- striated muscle cell differentiation Source: MGI
- synapse assembly Source: MGI
- telencephalon development Source: MGI
Keywordsi
Biological process | Cell adhesion |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-418990, Adherens junctions interactions R-MMU-525793, Myogenesis R-MMU-8957275, Post-translational protein phosphorylation |
Names & Taxonomyi
Protein namesi | Recommended name: Cadherin-2Alternative name(s): Neural cadherin Short name: N-cadherin CD_antigen: CD325 |
Gene namesi | Name:Cdh2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88355, Cdh2 |
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; Single-pass type I membrane protein Sequence analysis
- sarcolemma 1 Publication
Other locations
- Cell junction 1 Publication
- Cell surface 2 Publications
Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes (PubMed:26403541). Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes (PubMed:23392350).2 Publications
Cytoskeleton
- cortical actin cytoskeleton Source: Ensembl
Plasma Membrane
- apical plasma membrane Source: MGI
- apicolateral plasma membrane Source: MGI
- basolateral plasma membrane Source: MGI
- catenin complex Source: MGI
- integral component of plasma membrane Source: UniProtKB
- integral component of postsynaptic specialization membrane Source: MGI
- integral component of presynaptic active zone membrane Source: MGI
- plasma membrane Source: UniProtKB
- plasma membrane raft Source: UniProtKB
- sarcolemma Source: UniProtKB
- T-tubule Source: MGI
Other locations
- adherens junction Source: MGI
- apical part of cell Source: GO_Central
- cell junction Source: MGI
- cell surface Source: UniProtKB
- cell-cell junction Source: BHF-UCL
- cytoplasm Source: MGI
- fascia adherens Source: MGI
- glutamatergic synapse Source: MGI
- intercalated disc Source: UniProtKB
- lamellipodium Source: MGI
- membrane Source: MGI
- neuron projection Source: GO_Central
- postsynaptic density Source: SynGO
- protein-containing complex Source: MGI
- synapse Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 160 – 724 | ExtracellularSequence analysisAdd BLAST | 565 | |
Transmembranei | 725 – 745 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 746 – 906 | CytoplasmicSequence analysisAdd BLAST | 161 |
Keywords - Cellular componenti
Cell junction, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 161 | W → A or Y: Loss of calcium-dependent cell-cell adhesion. 1 Publication | 1 | |
Mutagenesisi | 161 | W → A: Loss of dimerization; when associated with E-173. 1 Publication | 1 | |
Mutagenesisi | 162 | V → A: Decreased calcium-dependent cell-cell adhesion. 1 Publication | 1 | |
Mutagenesisi | 173 | R → E: Loss of dimerization; when associated with A-161. 1 Publication | 1 | |
Mutagenesisi | 237 | A → M: Loss of calcium-dependent cell-cell adhesion. 1 Publication | 1 | |
Mutagenesisi | 239 | A → M: Loss of calcium-dependent cell-cell adhesion. 1 Publication | 1 | |
Mutagenesisi | 262 | D → A: Abolishes dimerization. 1 Publication | 1 | |
Mutagenesisi | 293 | D → A: Severely impaired the binding of calcium to all three sites. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
PropeptideiPRO_0000003733 | 26 – 159 | Sequence analysisAdd BLAST | 134 | |
ChainiPRO_0000003734 | 160 – 906 | Cadherin-2Add BLAST | 747 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 190 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 273 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 325 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 402 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 572 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 651 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 692 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Cleavage on pair of basic residues, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | P15116 |
MaxQBi | P15116 |
PaxDbi | P15116 |
PeptideAtlasi | P15116 |
PRIDEi | P15116 |
PTM databases
GlyConnecti | 2169, 7 N-Linked glycans (3 sites) |
GlyGeni | P15116, 16 sites |
iPTMneti | P15116 |
PhosphoSitePlusi | P15116 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000024304, Expressed in cardiac ventricle and 408 other tissues |
ExpressionAtlasi | P15116, baseline and differential |
Genevisiblei | P15116, MM |
Interactioni
Subunit structurei
Homodimer (via extracellular region) (PubMed:21300292, PubMed:25253890). Can also form heterodimers with other cadherins (via extracellular region) (PubMed:25253890). Dimerization occurs in trans, i.e. with a cadherin chain from another cell (PubMed:21300292, PubMed:25253890).
Interacts with CDCP1 (By similarity).
Interacts with PCDH8; this complex may also include TAOK2 (By similarity). The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway (By similarity).
Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN (PubMed:11433297). May interact with OBSCN (via protein kinase domain 2) (PubMed:23392350).
By similarity4 PublicationsBinary interactionsi
Hide detailsP15116
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-397974,EBI-397974 |
PTPN1 [P18031] from Homo sapiens. | 3 | EBI-397974,EBI-968788 |
GO - Molecular functioni
- alpha-catenin binding Source: MGI
- beta-catenin binding Source: MGI
- cadherin binding Source: GO_Central
- enzyme binding Source: UniProtKB
- gamma-catenin binding Source: MGI
- identical protein binding Source: IntAct
- nitric-oxide synthase binding Source: MGI
- protein kinase binding Source: UniProtKB
- protein phosphatase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 198637, 34 interactors |
CORUMi | P15116 |
DIPi | DIP-31564N |
IntActi | P15116, 18 interactors |
MINTi | P15116 |
STRINGi | 10090.ENSMUSP00000025166 |
Miscellaneous databases
RNActi | P15116, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P15116 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P15116 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 160 – 267 | Cadherin 1PROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 268 – 382 | Cadherin 2PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 383 – 497 | Cadherin 3PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 498 – 603 | Cadherin 4PROSITE-ProRule annotationAdd BLAST | 106 | |
Domaini | 604 – 717 | Cadherin 5PROSITE-ProRule annotationAdd BLAST | 114 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 863 – 878 | Ser-richAdd BLAST | 16 |
Domaini
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3594, Eukaryota |
GeneTreei | ENSGT00940000155981 |
InParanoidi | P15116 |
OrthoDBi | 191117at2759 |
TreeFami | TF316817 |
Family and domain databases
Gene3Di | 4.10.900.10, 1 hit |
InterProi | View protein in InterPro IPR039808, Cadherin IPR002126, Cadherin-like_dom IPR015919, Cadherin-like_sf IPR020894, Cadherin_CS IPR000233, Cadherin_cytoplasmic-dom IPR014868, Cadherin_pro_dom IPR027397, Catenin_binding_dom_sf IPR030051, CDH2 |
PANTHERi | PTHR24027, PTHR24027, 1 hit PTHR24027:SF79, PTHR24027:SF79, 1 hit |
Pfami | View protein in Pfam PF00028, Cadherin, 5 hits PF01049, Cadherin_C, 1 hit PF08758, Cadherin_pro, 1 hit |
PRINTSi | PR00205, CADHERIN |
SMARTi | View protein in SMART SM00112, CA, 5 hits SM01055, Cadherin_pro, 1 hit |
SUPFAMi | SSF49313, SSF49313, 6 hits |
PROSITEi | View protein in PROSITE PS00232, CADHERIN_1, 3 hits PS50268, CADHERIN_2, 5 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MCRIAGAPRT LLPLLAALLQ ASVEASGEIA LCKTGFPEDV YSAVLPKDVH
60 70 80 90 100
EGQPLLNVKF SNCNRKRKVQ YESSEPADFK VDEDGTVYAV RSFPLTAEQA
110 120 130 140 150
KFLIYAQDKE TQEKWQVAVN LSREPTLTEE PMKEPHEIEE IVFPRQLAKH
160 170 180 190 200
SGALQRQKRD WVIPPINLPE NSRGPFPQEL VRIRSDRDKN LSLRYSVTGP
210 220 230 240 250
GADQPPTGIF IINPISGQLS VTKPLDRELI ARFHLRAHAV DINGNQVENP
260 270 280 290 300
IDIVINVIDM NDNRPEFLHQ VWNGSVPEGS KPGTYVMTVT AIDADDPNAL
310 320 330 340 350
NGMLRYRILS QAPSTPSPNM FTINNETGDI ITVAAGLDRE KVQQYTLIIQ
360 370 380 390 400
ATDMEGNPTY GLSNTATAVI TVTDVNDNPP EFTAMTFYGE VPENRVDVIV
410 420 430 440 450
ANLTVTDKDQ PHTPAWNAAY RISGGDPTGR FAILTDPNSN DGLVTVVKPI
460 470 480 490 500
DFETNRMFVL TVAAENQVPL AKGIQHPPQS TATVSVTVID VNENPYFAPN
510 520 530 540 550
PKIIRQEEGL HAGTMLTTLT AQDPDRYMQQ NIRYTKLSDP ANWLKIDPVN
560 570 580 590 600
GQITTIAVLD RESPNVKNNI YNATFLASDN GIPPMSGTGT LQIYLLDIND
610 620 630 640 650
NAPQVLPQEA ETCETPEPNS INITALDYDI DPNAGPFAFD LPLSPVTIKR
660 670 680 690 700
NWTINRLNGD FAQLNLKIKF LEAGIYEVPI IITDSGNPPK SNISILRVKV
710 720 730 740 750
CQCDSNGDCT DVDRIVGAGL GTGAIIAILL CIIILLILVL MFVVWMKRRD
760 770 780 790 800
KERQAKQLLI DPEDDVRDNI LKYDEEGGGE EDQDYDLSQL QQPDTVEPDA
810 820 830 840 850
IKPVGIRRLD ERPIHAEPQY PVRSAAPHPG DIGDFINEGL KAADNDPTAP
860 870 880 890 900
PYDSLLVFDY EGSGSTAGSL SSLNSSSSGG DQDYDYLNDW GPRFKKLADM
YGGGDD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3YYT0 | D3YYT0_MOUSE | Cadherin-2 | Cdh2 | 849 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 7 – 9 | APR → GRG in AAA37353 (PubMed:2762814).Curated | 3 | |
Sequence conflicti | 565 – 567 | NVK → YVQ in AAA37353 (PubMed:2762814).Curated | 3 | |
Sequence conflicti | 624 | T → A in AAA37353 (PubMed:2762814).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31131 mRNA Translation: AAA37353.1 AB008811 mRNA Translation: BAA23549.1 S45011 Genomic DNA Translation: AAB23356.1 CH466557 Genomic DNA Translation: EDK96930.1 BC022107 mRNA Translation: AAH22107.1 |
CCDSi | CCDS29076.1 |
PIRi | A32759, IJMSCN |
RefSeqi | NP_031690.3, NM_007664.5 |
Genome annotation databases
Ensembli | ENSMUST00000025166; ENSMUSP00000025166; ENSMUSG00000024304 |
GeneIDi | 12558 |
KEGGi | mmu:12558 |
UCSCi | uc008edx.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31131 mRNA Translation: AAA37353.1 AB008811 mRNA Translation: BAA23549.1 S45011 Genomic DNA Translation: AAB23356.1 CH466557 Genomic DNA Translation: EDK96930.1 BC022107 mRNA Translation: AAH22107.1 |
CCDSi | CCDS29076.1 |
PIRi | A32759, IJMSCN |
RefSeqi | NP_031690.3, NM_007664.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NCG | X-ray | 2.10 | A | 160-267 | [»] | |
1NCH | X-ray | 2.10 | A/B | 160-267 | [»] | |
1NCI | X-ray | 2.10 | A/B | 160-267 | [»] | |
1NCJ | X-ray | 3.40 | A | 160-374 | [»] | |
1OP4 | NMR | - | A | 24-159 | [»] | |
2QVI | X-ray | 3.01 | A | 160-374 | [»] | |
3Q2W | X-ray | 3.20 | A | 160-712 | [»] | |
4NUM | X-ray | 3.30 | A/B/C/D | 160-374 | [»] | |
4NUP | X-ray | 2.70 | A/B/C | 162-374 | [»] | |
4NUQ | X-ray | 2.12 | A | 160-374 | [»] | |
SMRi | P15116 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198637, 34 interactors |
CORUMi | P15116 |
DIPi | DIP-31564N |
IntActi | P15116, 18 interactors |
MINTi | P15116 |
STRINGi | 10090.ENSMUSP00000025166 |
PTM databases
GlyConnecti | 2169, 7 N-Linked glycans (3 sites) |
GlyGeni | P15116, 16 sites |
iPTMneti | P15116 |
PhosphoSitePlusi | P15116 |
Proteomic databases
jPOSTi | P15116 |
MaxQBi | P15116 |
PaxDbi | P15116 |
PeptideAtlasi | P15116 |
PRIDEi | P15116 |
Protocols and materials databases
Antibodypediai | 4558, 1639 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025166; ENSMUSP00000025166; ENSMUSG00000024304 |
GeneIDi | 12558 |
KEGGi | mmu:12558 |
UCSCi | uc008edx.2, mouse |
Organism-specific databases
CTDi | 1000 |
MGIi | MGI:88355, Cdh2 |
Phylogenomic databases
eggNOGi | KOG3594, Eukaryota |
GeneTreei | ENSGT00940000155981 |
InParanoidi | P15116 |
OrthoDBi | 191117at2759 |
TreeFami | TF316817 |
Enzyme and pathway databases
Reactomei | R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-418990, Adherens junctions interactions R-MMU-525793, Myogenesis R-MMU-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 12558, 0 hits in 17 CRISPR screens |
ChiTaRSi | Cdh2, mouse |
EvolutionaryTracei | P15116 |
PROi | PR:P15116 |
RNActi | P15116, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024304, Expressed in cardiac ventricle and 408 other tissues |
ExpressionAtlasi | P15116, baseline and differential |
Genevisiblei | P15116, MM |
Family and domain databases
Gene3Di | 4.10.900.10, 1 hit |
InterProi | View protein in InterPro IPR039808, Cadherin IPR002126, Cadherin-like_dom IPR015919, Cadherin-like_sf IPR020894, Cadherin_CS IPR000233, Cadherin_cytoplasmic-dom IPR014868, Cadherin_pro_dom IPR027397, Catenin_binding_dom_sf IPR030051, CDH2 |
PANTHERi | PTHR24027, PTHR24027, 1 hit PTHR24027:SF79, PTHR24027:SF79, 1 hit |
Pfami | View protein in Pfam PF00028, Cadherin, 5 hits PF01049, Cadherin_C, 1 hit PF08758, Cadherin_pro, 1 hit |
PRINTSi | PR00205, CADHERIN |
SMARTi | View protein in SMART SM00112, CA, 5 hits SM01055, Cadherin_pro, 1 hit |
SUPFAMi | SSF49313, SSF49313, 6 hits |
PROSITEi | View protein in PROSITE PS00232, CADHERIN_1, 3 hits PS50268, CADHERIN_2, 5 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CADH2_MOUSE | |
Accessioni | P15116Primary (citable) accession number: P15116 Secondary accession number(s): Q64260, Q6GU11 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references