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Protein

Glutamine synthetase

Gene

Glul

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity).By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Protein has several cofactor binding sites:

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Lyase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2 3474
ReactomeiR-MMU-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
R-MMU-70614 Amino acid synthesis and interconversion (transamination)

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Short name:
GS
Alternative name(s):
Glutamate decarboxylase (EC:4.1.1.15)
Glutamate--ammonia ligase
Gene namesi
Name:Glul
Synonyms:Glns
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:95739 Glul

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001531412 – 373Glutamine synthetaseAdd BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei104PhosphotyrosineCombined sources1
Modified residuei343PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by ZNRF1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP15105
MaxQBiP15105
PaxDbiP15105
PeptideAtlasiP15105
PRIDEiP15105

2D gel databases

REPRODUCTION-2DPAGEiIPI00626790
P15105
SWISS-2DPAGEiP15105
UCD-2DPAGEiP15105

PTM databases

iPTMnetiP15105
PhosphoSitePlusiP15105
SwissPalmiP15105

Expressioni

Gene expression databases

BgeeiENSMUSG00000026473
CleanExiMM_GLUL
ExpressionAtlasiP15105 baseline and differential
GenevisibleiP15105 MM

Interactioni

Subunit structurei

Homooctamer and homotetramer. Interacts with PALMD.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199947, 11 interactors
IntActiP15105, 18 interactors
MINTiP15105
STRINGi10090.ENSMUSP00000083375

Structurei

3D structure databases

ProteinModelPortaliP15105
SMRiP15105
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiKOG0683 Eukaryota
COG0174 LUCA
GeneTreeiENSGT00390000010047
HOGENOMiHOG000061500
HOVERGENiHBG005847
InParanoidiP15105
KOiK01915
OMAiMMPDGVT
OrthoDBiEOG091G07E9
PhylomeDBiP15105
TreeFamiTF300491

Family and domain databases

Gene3Di3.10.20.70, 1 hit
InterProiView protein in InterPro
IPR008147 Gln_synt_b-grasp
IPR036651 Gln_synt_N
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR008146 Gln_synth_cat_dom
IPR027303 Gln_synth_gly_rich_site
IPR027302 Gln_synth_N_conserv_site
PfamiView protein in Pfam
PF00120 Gln-synt_C, 1 hit
PF03951 Gln-synt_N, 1 hit
SMARTiView protein in SMART
SM01230 Gln-synt_C, 1 hit
SUPFAMiSSF54368 SSF54368, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00180 GLNA_1, 1 hit
PS00181 GLNA_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSASSHLN KGIKQMYMSL PQGEKVQAMY IWVDGTGEGL RCKTRTLDCE
60 70 80 90 100
PKCVEELPEW NFDGSSTFQS EGSNSDMYLH PVAMFRDPFR KDPNKLVLCE
110 120 130 140 150
VFKYNRKPAE TNLRHICKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP
160 170 180 190 200
SNGFPGPQGP YYCGVGADKA YGRDIVEAHY RACLYAGVKI TGTNAEVMPA
210 220 230 240 250
QWEFQIGPCE GIRMGDHLWI ARFILHRVCE DFGVIATFDP KPIPGNWNGA
260 270 280 290 300
GCHTNFSTKA MREENGLKCI EEAIDKLSKR HQYHIRAYDP KGGLDNARRL
310 320 330 340 350
TGFHETSNIN DFSAGVANRG ASIRIPRTVG QEKKGYFEDR RPSANCDPYA
360 370
VTEAIVRTCL LNETGDEPFQ YKN
Length:373
Mass (Da):42,120
Last modified:April 17, 2007 - v6
Checksum:i1EC9CDC5D81DE63F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91K → R in CAA34381 (PubMed:2475638).Curated1
Sequence conflicti111T → S in AAA17989 (Ref. 2) Curated1
Sequence conflicti133M → L in BAE37022 (PubMed:16141072).Curated1
Sequence conflicti249G → V in CAA34381 (PubMed:2475638).Curated1
Sequence conflicti269C → W in AAA17989 (Ref. 2) Curated1
Sequence conflicti299R → A in CAA34381 (PubMed:2475638).Curated1
Sequence conflicti342 – 343PS → LR in CAA34381 (PubMed:2475638).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16314 mRNA Translation: CAA34381.1
U09114 mRNA Translation: AAA17989.1
AY044241 mRNA Translation: AAK95328.1
AK159106 mRNA Translation: BAE34822.1
AK160670 mRNA Translation: BAE35950.1
AK162685 mRNA Translation: BAE37022.1
AK168493 mRNA Translation: BAE40380.1
BC015086 mRNA Translation: AAH15086.1
CCDSiCCDS15381.1
PIRiS04991 AJMSQ
RefSeqiNP_032157.2, NM_008131.4
UniGeneiMm.210745

Genome annotation databases

EnsembliENSMUST00000086199; ENSMUSP00000083375; ENSMUSG00000026473
GeneIDi14645
KEGGimmu:14645
UCSCiuc007daq.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGLNA_MOUSE
AccessioniPrimary (citable) accession number: P15105
Secondary accession number(s): Q3TRK7, Q64432, Q91VC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 17, 2007
Last modified: June 20, 2018
This is version 166 of the entry and version 6 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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