UniProtKB - P15085 (CBPA1_HUMAN)
Protein
Carboxypeptidase A1
Gene
CPA1
Organism
Homo sapiens (Human)
Status
Functioni
Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed:8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).By similarity1 Publication
Catalytic activityi
- This reaction proceeds in the forwardBy similarity direction.
- Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.1 Publication EC:3.4.17.1
Cofactori
Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 179 | Zinc; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 182 | Zinc; catalyticCombined sources1 Publication | 1 | |
Binding sitei | 237 | SubstrateCombined sources1 Publication | 1 | |
Metal bindingi | 306 | Zinc; catalyticCombined sources1 Publication | 1 | |
Binding sitei | 358 | SubstrateCombined sources1 Publication | 1 | |
Active sitei | 380 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- metallocarboxypeptidase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- leukotriene metabolic process Source: UniProtKB
- proteolysis Source: GO_Central
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P15085 |
Protein family/group databases
MEROPSi | M14.001 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2296, CPA1 |
MIMi | 114850, gene |
neXtProti | NX_P15085 |
VEuPathDBi | HostDB:ENSG00000091704.9 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: UniProtKB
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 1357 |
GeneReviewsi | CPA1 |
MalaCardsi | CPA1 |
OpenTargetsi | ENSG00000091704 |
Orphaneti | 676, Hereditary chronic pancreatitis |
PharmGKBi | PA26816 |
Miscellaneous databases
Pharosi | P15085, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2088 |
DrugBanki | DB06924, (2R)-2-benzyl-3-nitropropanoic acid DB07484, (2R)-4,4-dihydroxy-5-nitro-2-(phenylmethyl)pentanoic acid DB02494, (S)-3-phenyllactic acid DB03441, 2-Benzyl-3-Iodopropanoic Acid DB04316, D-[(N-Hydroxyamino)Carbonyl]Phenylalanine DB03201, D-Cysteine DB11095, Desirudin DB02652, L-[(N-Hydroxyamino)Carbonyl]Phenylalanine DB07506, L-BENZYLSUCCINIC ACID DB04058, N-Carbamoylphenylalanine DB07351, O-(((1R)-((N-(PHENYL-METHOXY-CARBONYL)-ALANYL)-AMINO)METHYL)HYDROXYPHOSPHINYL)3-L-PHENYLLACTATE DB08762, O-(((1R)-((N-PHENYLMETHOXYCARBONYL-L-ALANYL)AMINO)ETHYL)HYDROXYPHOSPHONO)-L-BENZYLACETIC ACID DB08368, OCTANE-1,3,5,7-TETRACARBOXYLIC ACID DB03012, Phenylalanine-N-Sulfonamide |
GuidetoPHARMACOLOGYi | 1587 |
Genetic variation databases
BioMutai | CPA1 |
DMDMi | 399196 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | 2 PublicationsAdd BLAST | 16 | |
PropeptideiPRO_0000004345 | 17 – 110 | Activation peptideAdd BLAST | 94 | |
ChainiPRO_0000004346 | 111 – 419 | Carboxypeptidase A1Add BLAST | 309 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 248 ↔ 271 | 2 Publications |
Keywords - PTMi
Disulfide bond, ZymogenProteomic databases
jPOSTi | P15085 |
MassIVEi | P15085 |
MaxQBi | P15085 |
PaxDbi | P15085 |
PeptideAtlasi | P15085 |
PRIDEi | P15085 |
ProteomicsDBi | 53103 |
PTM databases
iPTMneti | P15085 |
PhosphoSitePlusi | P15085 |
Expressioni
Gene expression databases
Bgeei | ENSG00000091704, Expressed in body of pancreas and 104 other tissues |
ExpressionAtlasi | P15085, baseline and differential |
Genevisiblei | P15085, HS |
Organism-specific databases
HPAi | ENSG00000091704, Tissue enriched (pancreas) |
Interactioni
Subunit structurei
Monomer. May form a complex with proelastase 2.
2 PublicationsBinary interactionsi
P15085
With | #Exp. | IntAct |
---|---|---|
P19399 from Ascaris suum. | 2 | EBI-1642148,EBI-15754788 |
Protein-protein interaction databases
BioGRIDi | 107749, 1 interactor |
DIPi | DIP-48699N |
IntActi | P15085, 3 interactors |
STRINGi | 9606.ENSP00000011292 |
Chemistry databases
BindingDBi | P15085 |
Miscellaneous databases
RNActi | P15085, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P15085 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P15085 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 179 – 182 | Substrate bindingCombined sources1 Publication | 4 | |
Regioni | 254 – 255 | Substrate bindingCombined sources1 Publication | 2 | |
Regioni | 307 – 308 | Substrate bindingCombined sources1 Publication | 2 |
Sequence similaritiesi
Belongs to the peptidase M14 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2650, Eukaryota |
GeneTreei | ENSGT00940000158082 |
InParanoidi | P15085 |
OMAi | SHGISYE |
OrthoDBi | 524270at2759 |
PhylomeDBi | P15085 |
TreeFami | TF317197 |
Family and domain databases
CDDi | cd03870, M14_CPA, 1 hit |
Gene3Di | 3.30.70.340, 1 hit |
InterProi | View protein in InterPro IPR034248, CPA_M14_CPD IPR036990, M14A-like_propep IPR003146, M14A_act_pep IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF00246, Peptidase_M14, 1 hit PF02244, Propep_M14, 1 hit |
PRINTSi | PR00765, CRBOXYPTASEA |
SMARTi | View protein in SMART SM00631, Zn_pept, 1 hit |
PROSITEi | View protein in PROSITE PS00132, CARBOXYPEPT_ZN_1, 1 hit PS00133, CARBOXYPEPT_ZN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
P15085-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRGLLVLSVL LGAVFGKEDF VGHQVLRISV ADEAQVQKVK ELEDLEHLQL
60 70 80 90 100
DFWRGPAHPG SPIDVRVPFP SIQAVKIFLE SHGISYETMI EDVQSLLDEE
110 120 130 140 150
QEQMFAFRSR ARSTDTFNYA TYHTLEEIYD FLDLLVAENP HLVSKIQIGN
160 170 180 190 200
TYEGRPIYVL KFSTGGSKRP AIWIDTGIHS REWVTQASGV WFAKKITQDY
210 220 230 240 250
GQDAAFTAIL DTLDIFLEIV TNPDGFAFTH STNRMWRKTR SHTAGSLCIG
260 270 280 290 300
VDPNRNWDAG FGLSGASSNP CSETYHGKFA NSEVEVKSIV DFVKDHGNIK
310 320 330 340 350
AFISIHSYSQ LLMYPYGYKT EPVPDQDELD QLSKAAVTAL ASLYGTKFNY
360 370 380 390 400
GSIIKAIYQA SGSTIDWTYS QGIKYSFTFE LRDTGRYGFL LPASQIIPTA
410
KETWLALLTI MEHTLNHPY
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketS4R433 | S4R433_HUMAN | Carboxypeptidase A1 | CPA1 | 191 | Annotation score: | ||
C9JUF9 | C9JUF9_HUMAN | Carboxypeptidase A1 | CPA1 | 331 | Annotation score: | ||
C9JUZ4 | C9JUZ4_HUMAN | Carboxypeptidase A1 | CPA1 | 269 | Annotation score: | ||
C9JQ63 | C9JQ63_HUMAN | Carboxypeptidase A1 | CPA1 | 103 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 139 – 141 | NPH → HPG AA sequence (PubMed:8318831).Curated | 3 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048593 | 208 | A → T. Corresponds to variant dbSNP:rs34474469EnsemblClinVar. | 1 | |
Natural variantiVAR_054311 | 276 | H → R2 PublicationsCorresponds to variant dbSNP:rs17849959Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67318 mRNA Translation: CAA47732.1 AK291493 mRNA Translation: BAF84182.1 BT007313 mRNA Translation: AAP35977.1 CH236950 Genomic DNA Translation: EAL24089.1 CH471070 Genomic DNA Translation: EAW83763.1 BC005279 mRNA Translation: AAH05279.1 |
CCDSi | CCDS5820.1 |
PIRi | S29127 |
RefSeqi | NP_001859.1, NM_001868.3 |
Genome annotation databases
Ensembli | ENST00000011292; ENSP00000011292; ENSG00000091704 |
GeneIDi | 1357 |
KEGGi | hsa:1357 |
UCSCi | uc003vpx.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67318 mRNA Translation: CAA47732.1 AK291493 mRNA Translation: BAF84182.1 BT007313 mRNA Translation: AAP35977.1 CH236950 Genomic DNA Translation: EAL24089.1 CH471070 Genomic DNA Translation: EAW83763.1 BC005279 mRNA Translation: AAH05279.1 |
CCDSi | CCDS5820.1 |
PIRi | S29127 |
RefSeqi | NP_001859.1, NM_001868.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2V77 | X-ray | 1.60 | A/B | 111-419 | [»] | |
3FJU | X-ray | 1.60 | A | 112-418 | [»] | |
4UEE | X-ray | 2.27 | A/B | 111-419 | [»] | |
4UEF | X-ray | 1.69 | A/B | 111-419 | [»] | |
4UEZ | X-ray | 2.29 | A/B | 111-419 | [»] | |
4UF4 | X-ray | 1.77 | A/B | 1-419 | [»] | |
5OM9 | X-ray | 1.80 | A/B | 1-419 | [»] | |
6I6Z | X-ray | 1.72 | A/B | 111-418 | [»] | |
SMRi | P15085 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107749, 1 interactor |
DIPi | DIP-48699N |
IntActi | P15085, 3 interactors |
STRINGi | 9606.ENSP00000011292 |
Chemistry databases
BindingDBi | P15085 |
ChEMBLi | CHEMBL2088 |
DrugBanki | DB06924, (2R)-2-benzyl-3-nitropropanoic acid DB07484, (2R)-4,4-dihydroxy-5-nitro-2-(phenylmethyl)pentanoic acid DB02494, (S)-3-phenyllactic acid DB03441, 2-Benzyl-3-Iodopropanoic Acid DB04316, D-[(N-Hydroxyamino)Carbonyl]Phenylalanine DB03201, D-Cysteine DB11095, Desirudin DB02652, L-[(N-Hydroxyamino)Carbonyl]Phenylalanine DB07506, L-BENZYLSUCCINIC ACID DB04058, N-Carbamoylphenylalanine DB07351, O-(((1R)-((N-(PHENYL-METHOXY-CARBONYL)-ALANYL)-AMINO)METHYL)HYDROXYPHOSPHINYL)3-L-PHENYLLACTATE DB08762, O-(((1R)-((N-PHENYLMETHOXYCARBONYL-L-ALANYL)AMINO)ETHYL)HYDROXYPHOSPHONO)-L-BENZYLACETIC ACID DB08368, OCTANE-1,3,5,7-TETRACARBOXYLIC ACID DB03012, Phenylalanine-N-Sulfonamide |
GuidetoPHARMACOLOGYi | 1587 |
Protein family/group databases
MEROPSi | M14.001 |
PTM databases
iPTMneti | P15085 |
PhosphoSitePlusi | P15085 |
Genetic variation databases
BioMutai | CPA1 |
DMDMi | 399196 |
Proteomic databases
jPOSTi | P15085 |
MassIVEi | P15085 |
MaxQBi | P15085 |
PaxDbi | P15085 |
PeptideAtlasi | P15085 |
PRIDEi | P15085 |
ProteomicsDBi | 53103 |
Protocols and materials databases
Antibodypediai | 17964, 500 antibodies |
DNASUi | 1357 |
Genome annotation databases
Ensembli | ENST00000011292; ENSP00000011292; ENSG00000091704 |
GeneIDi | 1357 |
KEGGi | hsa:1357 |
UCSCi | uc003vpx.4, human |
Organism-specific databases
CTDi | 1357 |
DisGeNETi | 1357 |
GeneCardsi | CPA1 |
GeneReviewsi | CPA1 |
HGNCi | HGNC:2296, CPA1 |
HPAi | ENSG00000091704, Tissue enriched (pancreas) |
MalaCardsi | CPA1 |
MIMi | 114850, gene |
neXtProti | NX_P15085 |
OpenTargetsi | ENSG00000091704 |
Orphaneti | 676, Hereditary chronic pancreatitis |
PharmGKBi | PA26816 |
VEuPathDBi | HostDB:ENSG00000091704.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2650, Eukaryota |
GeneTreei | ENSGT00940000158082 |
InParanoidi | P15085 |
OMAi | SHGISYE |
OrthoDBi | 524270at2759 |
PhylomeDBi | P15085 |
TreeFami | TF317197 |
Enzyme and pathway databases
PathwayCommonsi | P15085 |
Miscellaneous databases
BioGRID-ORCSi | 1357, 3 hits in 981 CRISPR screens |
ChiTaRSi | CPA1, human |
EvolutionaryTracei | P15085 |
GeneWikii | Carboxypeptidase_A1 |
GenomeRNAii | 1357 |
Pharosi | P15085, Tchem |
PROi | PR:P15085 |
RNActi | P15085, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000091704, Expressed in body of pancreas and 104 other tissues |
ExpressionAtlasi | P15085, baseline and differential |
Genevisiblei | P15085, HS |
Family and domain databases
CDDi | cd03870, M14_CPA, 1 hit |
Gene3Di | 3.30.70.340, 1 hit |
InterProi | View protein in InterPro IPR034248, CPA_M14_CPD IPR036990, M14A-like_propep IPR003146, M14A_act_pep IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF00246, Peptidase_M14, 1 hit PF02244, Propep_M14, 1 hit |
PRINTSi | PR00765, CRBOXYPTASEA |
SMARTi | View protein in SMART SM00631, Zn_pept, 1 hit |
PROSITEi | View protein in PROSITE PS00132, CARBOXYPEPT_ZN_1, 1 hit PS00133, CARBOXYPEPT_ZN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CBPA1_HUMAN | |
Accessioni | P15085Primary (citable) accession number: P15085 Secondary accession number(s): A4D1M1 Q9UCF2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | July 1, 1993 | |
Last modified: | April 7, 2021 | |
This is version 189 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families