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Protein

Glycogen debranching enzyme

Gene

glgX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes maltotriose and maltotetraose chains that are attached by 1,6-alpha-linkage to the limit dextrin main chain, generating a debranched limit dextrin. Shows only very little activity with native glycogen.3 Publications

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages to branches with degrees of polymerization of three or four glucose residues in limit dextrin.UniRule annotation3 Publications

Activity regulationi

Inhibited by iodoacetate, p-chloromercuribenzoate, HgCl2, cupric sulfate and ammonium sulfate.1 Publication

pH dependencei

Optimum pH is 5.6 to 6.4.1 Publication

Temperature dependencei

Optimum temperature is 45-50 degrees Celsius.1 Publication

Pathwayi: glycogen degradation

This protein is involved in the pathway glycogen degradation, which is part of Glycan degradation.UniRule annotation2 Publications
View all proteins of this organism that are known to be involved in the pathway glycogen degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei336NucleophileUniRule annotation1 Publication1
Active sitei371Proton donorUniRule annotation1 Publication1
Sitei443Transition state stabilizerUniRule annotation1 Publication1

GO - Molecular functioni

  • amylo-alpha-1,6-glucosidase activity Source: EcoCyc
  • glycogen debranching enzyme activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • glycogen catabolic process Source: EcoCyc

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciEcoCyc:EG10381-MONOMER
MetaCyc:EG10381-MONOMER
BRENDAi3.2.1.33 2026
UniPathwayi
UPA00165

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzyme1 PublicationUniRule annotation (EC:3.2.1.196UniRule annotation3 Publications)
Alternative name(s):
Glycogen operon protein GlgX
Limit dextrin alpha-1,6-maltotetraose-hydrolaseUniRule annotationCurated
Gene namesi
Name:glgX2 PublicationsUniRule annotation
Synonyms:glyX
Ordered Locus Names:b3431, JW3394
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10381 glgX

Pathology & Biotechi

Disruption phenotypei

Disruption of the gene leads to overproduction of glycogen containing short external chains.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542971 – 657Glycogen debranching enzymeAdd BLAST657

Proteomic databases

PaxDbiP15067
PRIDEiP15067

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4263517, 21 interactors
IntActiP15067, 14 interactors
STRINGi316385.ECDH10B_3605

Structurei

Secondary structure

1657
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP15067
SMRiP15067
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15067

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4107SS7 Bacteria
COG1523 LUCA
HOGENOMiHOG000239197
InParanoidiP15067
KOiK02438
OMAiSEPWDCG
PhylomeDBiP15067

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
HAMAPiMF_01248 GlgX, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR022844 Glycogen_debranch_bac
IPR011837 Glycogen_debranch_GlgX
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02922 CBM_48, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR02100 glgX_debranch, 1 hit

Sequencei

Sequence statusi: Complete.

P15067-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP
60 70 80 90 100
GHSGDIWHGY LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI
110 120 130 140 150
DGEFKDNPLL HAGHNEPDYR DNAAIAPKCV VVVDHYDWED DAPPRTPWGS
160 170 180 190 200
TIIYEAHVKG LTYLHPEIPV EIRGTYKALG HPVMINYLKQ LGITALELLP
210 220 230 240 250
VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA LDEFRDAIKA
260 270 280 290 300
LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT
310 320 330 340 350
GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD
360 370 380 390 400
APLFTAIQNC PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA
410 420 430 440 450
RRFWLHYDLP LGAFAGRFAA SSDVFKRNGR LPSAAINLVT AHDGFTLRDC
460 470 480 490 500
VCFNHKHNEA NGEENRDGTN NNYSNNHGKE GLGGSLDLVE RRRDSIHALL
510 520 530 540 550
TTLLLSQGTP MLLAGDEHGH SQHGNNNAYC QDNQLTWLDW SQASSGLTAF
560 570 580 590 600
TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ
610 620 630 640 650
ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH

GLCVFQR
Length:657
Mass (Da):73,577
Last modified:November 1, 1995 - v3
Checksum:iAA9EFC1F67FD0420
GO

Sequence cautioni

The sequence AAA98735 differs from that shown. Reason: Erroneous termination at position 507. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti288Y → YY in AAA98735 (PubMed:2975249).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01616 Genomic DNA Translation: AAA98735.1 Sequence problems.
U18997 Genomic DNA Translation: AAA58229.1
U00096 Genomic DNA Translation: AAC76456.1
AP009048 Genomic DNA Translation: BAE77861.1
PIRiB65139 BVECGX
RefSeqiNP_417889.1, NC_000913.3
WP_000192523.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76456; AAC76456; b3431
BAE77861; BAE77861; BAE77861
GeneIDi947941
KEGGiecj:JW3394
eco:b3431
PATRICifig|511145.12.peg.3528

Similar proteinsi

Entry informationi

Entry nameiGLGX_ECOLI
AccessioniPrimary (citable) accession number: P15067
Secondary accession number(s): P76693, Q2M795
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1995
Last modified: September 12, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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