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Protein

ATP-dependent DNA helicase RecQ

Gene

recQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 54ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: EcoliWiki
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: GO_Central
  • ATP-dependent DNA helicase activity Source: EcoliWiki
  • DNA binding Source: EcoliWiki
  • DNA-dependent ATPase activity Source: EcoliWiki
  • four-way junction helicase activity Source: GO_Central
  • single-stranded DNA-dependent ATP-dependent DNA helicase activity Source: EcoliWiki
  • transition metal ion binding Source: EcoliWiki
  • zinc ion binding Source: EcoliWiki

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA duplex unwinding Source: GO_Central
  • DNA recombination Source: EcoliWiki
  • DNA repair Source: EcoliWiki
  • DNA replication Source: InterPro
  • SOS response Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10833-MONOMER
MetaCyc:EG10833-MONOMER
BRENDAi3.6.4.12 2026

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase RecQ (EC:3.6.4.12)
Gene namesi
Name:recQ
Ordered Locus Names:b3822, JW5855
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10833 recQ

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

DrugBankiDB02930 Phosphothiophosphoric Acid-Adenylate Ester

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002050332 – 609ATP-dependent DNA helicase RecQAdd BLAST608

Proteomic databases

PaxDbiP15043
PRIDEiP15043

Interactioni

Protein-protein interaction databases

BioGridi4259619, 148 interactors
DIPiDIP-10656N
IntActiP15043, 12 interactors
STRINGi316385.ECDH10B_4013

Structurei

Secondary structure

1609
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP15043
SMRiP15043
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15043

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 202Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini223 – 371Helicase C-terminalPROSITE-ProRule annotationAdd BLAST149
Domaini529 – 609HRDCPROSITE-ProRule annotationAdd BLAST81

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 149DEAH box4

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C9U Bacteria
COG0514 LUCA
HOGENOMiHOG000044388
InParanoidiP15043
KOiK03654
PhylomeDBiP15043

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR004589 DNA_helicase_ATP-dep_RecQ
IPR006293 DNA_helicase_ATP-dep_RecQ_bac
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR010997 HRDC-like_sf
IPR002121 HRDC_dom
IPR027417 P-loop_NTPase
IPR032284 RecQ_Zn-bd
IPR018982 RQC_domain
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF00570 HRDC, 1 hit
PF16124 RecQ_Zn_bind, 1 hit
PF09382 RQC, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00341 HRDC, 1 hit
SM00956 RQC, 1 hit
SUPFAMiSSF47819 SSF47819, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR01389 recQ, 1 hit
TIGR00614 recQ_fam, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50967 HRDC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15043-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQAEVLNLE SGAKQVLQET FGYQQFRPGQ EEIIDTVLSG RDCLVVMPTG
60 70 80 90 100
GGKSLCYQIP ALLLNGLTVV VSPLISLMKD QVDQLQANGV AAACLNSTQT
110 120 130 140 150
REQQLEVMTG CRTGQIRLLY IAPERLMLDN FLEHLAHWNP VLLAVDEAHC
160 170 180 190 200
ISQWGHDFRP EYAALGQLRQ RFPTLPFMAL TATADDTTRQ DIVRLLGLND
210 220 230 240 250
PLIQISSFDR PNIRYMLMEK FKPLDQLMRY VQEQRGKSGI IYCNSRAKVE
260 270 280 290 300
DTAARLQSKG ISAAAYHAGL ENNVRADVQE KFQRDDLQIV VATVAFGMGI
310 320 330 340 350
NKPNVRFVVH FDIPRNIESY YQETGRAGRD GLPAEAMLFY DPADMAWLRR
360 370 380 390 400
CLEEKPQGQL QDIERHKLNA MGAFAEAQTC RRLVLLNYFG EGRQEPCGNC
410 420 430 440 450
DICLDPPKQY DGSTDAQIAL STIGRVNQRF GMGYVVEVIR GANNQRIRDY
460 470 480 490 500
GHDKLKVYGM GRDKSHEHWV SVIRQLIHLG LVTQNIAQHS ALQLTEAARP
510 520 530 540 550
VLRGESSLQL AVPRIVALKP KAMQKSFGGN YDRKLFAKLR KLRKSIADES
560 570 580 590 600
NVPPYVVFND ATLIEMAEQM PITASEMLSV NGVGMRKLER FGKPFMALIR

AHVDGDDEE
Length:609
Mass (Da):68,364
Last modified:January 23, 2007 - v5
Checksum:i82542DE445655D48
GO

Sequence cautioni

The sequence AAA24517 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAA67618 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255R → A (PubMed:3027506).Curated1
Sequence conflicti255R → A (PubMed:1379743).Curated1
Sequence conflicti503 – 504RG → A (PubMed:3027506).Curated2
Sequence conflicti503 – 504RG → A (PubMed:1379743).Curated2
Sequence conflicti600R → P in AAA24517 (PubMed:3027506).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30198 Genomic DNA Translation: AAA24517.1 Different initiation.
M87049 Genomic DNA Translation: AAA67618.1 Different initiation.
U00096 Genomic DNA Translation: AAT48221.1
AP009048 Genomic DNA Translation: BAE77479.1
RefSeqiWP_000035581.1, NZ_LN832404.1
YP_026263.3, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48221; AAT48221; b3822
BAE77479; BAE77479; BAE77479
GeneIDi948318
KEGGiecj:JW5855
eco:b3822
PATRICifig|511145.12.peg.3938

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30198 Genomic DNA Translation: AAA24517.1 Different initiation.
M87049 Genomic DNA Translation: AAA67618.1 Different initiation.
U00096 Genomic DNA Translation: AAT48221.1
AP009048 Genomic DNA Translation: BAE77479.1
RefSeqiWP_000035581.1, NZ_LN832404.1
YP_026263.3, NC_000913.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OYWX-ray1.80A1-522[»]
1OYYX-ray2.50A1-523[»]
1WUDX-ray2.20A/B/D524-609[»]
ProteinModelPortaliP15043
SMRiP15043
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259619, 148 interactors
DIPiDIP-10656N
IntActiP15043, 12 interactors
STRINGi316385.ECDH10B_4013

Chemistry databases

DrugBankiDB02930 Phosphothiophosphoric Acid-Adenylate Ester

Proteomic databases

PaxDbiP15043
PRIDEiP15043

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48221; AAT48221; b3822
BAE77479; BAE77479; BAE77479
GeneIDi948318
KEGGiecj:JW5855
eco:b3822
PATRICifig|511145.12.peg.3938

Organism-specific databases

EchoBASEiEB0826
EcoGeneiEG10833 recQ

Phylogenomic databases

eggNOGiENOG4105C9U Bacteria
COG0514 LUCA
HOGENOMiHOG000044388
InParanoidiP15043
KOiK03654
PhylomeDBiP15043

Enzyme and pathway databases

BioCyciEcoCyc:EG10833-MONOMER
MetaCyc:EG10833-MONOMER
BRENDAi3.6.4.12 2026

Miscellaneous databases

EvolutionaryTraceiP15043
PROiPR:P15043

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR004589 DNA_helicase_ATP-dep_RecQ
IPR006293 DNA_helicase_ATP-dep_RecQ_bac
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR010997 HRDC-like_sf
IPR002121 HRDC_dom
IPR027417 P-loop_NTPase
IPR032284 RecQ_Zn-bd
IPR018982 RQC_domain
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF00570 HRDC, 1 hit
PF16124 RecQ_Zn_bind, 1 hit
PF09382 RQC, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00341 HRDC, 1 hit
SM00956 RQC, 1 hit
SUPFAMiSSF47819 SSF47819, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR01389 recQ, 1 hit
TIGR00614 recQ_fam, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50967 HRDC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRECQ_ECOLI
AccessioniPrimary (citable) accession number: P15043
Secondary accession number(s): P76762, Q2M8C7, Q6BEZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 180 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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