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Protein

DNA helicase IV

Gene

helD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei503ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi216 – 223ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: EcoCyc
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA recombination Source: EcoCyc

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10426-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase IV (EC:3.6.4.12)
Alternative name(s):
75 kDa helicase
Gene namesi
Name:helD
Ordered Locus Names:b0962, JW0945
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10426 helD

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • DNA helicase complex Source: EcoCyc

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4405

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020491 – 684DNA helicase IVAdd BLAST684

Proteomic databases

PaxDbiP15038
PRIDEiP15038

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4263362, 127 interactors
DIPiDIP-9876N
IntActiP15038, 35 interactors
STRINGi316385.ECDH10B_1032

Chemistry databases

BindingDBiP15038

Structurei

3D structure databases

ProteinModelPortaliP15038
SMRiP15038
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini195 – 505UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST311

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated

Phylogenomic databases

eggNOGiENOG41088T4 Bacteria
COG0210 LUCA
HOGENOMiHOG000118816
InParanoidiP15038
KOiK03658
OMAiDVSPQIV
PhylomeDBiP15038

Family and domain databases

InterProiView protein in InterPro
IPR014017 DNA_helicase_UvrD-like_C
IPR000212 DNA_helicase_UvrD/REP
IPR022161 Helicase_IV_N
IPR027417 P-loop_NTPase
IPR014016 UvrD-like_ATP-bd
IPR034739 UvrD/AddA_N
PANTHERiPTHR11070 PTHR11070, 1 hit
PfamiView protein in Pfam
PF12462 Helicase_IV_N, 1 hit
PF00580 UvrD-helicase, 1 hit
PF13361 UvrD_C, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51198 UVRD_HELICASE_ATP_BIND, 1 hit

Sequencei

Sequence statusi: Complete.

P15038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKATTLGK RLAQHPYDRA VILNAGIKVS GDRHEYLIPF NQLLAIHCKR
60 70 80 90 100
GLVWGELEFV LPDEKVVRLH GTEWGETQRF YHHLDAHWRR WSGEMSEIAS
110 120 130 140 150
GVLRQQLDLI ATRTGENKWL TREQTSGVQQ QIRQALSALP LPVNRLEEFD
160 170 180 190 200
NCREAWRKCQ AWLKDIESAR LQHNQAYTEA MLTEYADFFR QVESSPLNPA
210 220 230 240 250
QARAVVNGEH SLLVLAGAGS GKTSVLVARA GWLLARGEAS PEQILLLAFG
260 270 280 290 300
RKAAEEMDER IRERLHTEDI TARTFHALAL HIIQQGSKKV PIVSKLENDT
310 320 330 340 350
AARHELFIAE WRKQCSEKKA QAKGWRQWLT EEMQWSVPEG NFWDDEKLQR
360 370 380 390 400
RLASRLDRWV SLMRMHGGAQ AEMIASAPEE IRDLFSKRIK LMAPLLKAWK
410 420 430 440 450
GALKAENAVD FSGLIHQAIV ILEKGRFISP WKHILVDEFQ DISPQRAALL
460 470 480 490 500
AALRKQNSQT TLFAVGDDWQ AIYRFSGAQM SLTTAFHENF GEGERCDLDT
510 520 530 540 550
TYRFNSRIGE VANRFIQQNP GQLKKPLNSL TNGDKKAVTL LDESQLDALL
560 570 580 590 600
DKLSGYAKPE ERILILARYH HMRPASLEKA ATRWPKLQID FMTIHASKGQ
610 620 630 640 650
QADYVIIVGL QEGSDGFPAA ARESIMEEAL LPPVEDFPDA EERRLMYVAL
660 670 680
TRARHRVWAL FNKENPSPFV EILKNLDVPV ARKP
Length:684
Mass (Da):77,976
Last modified:November 1, 1997 - v2
Checksum:iFC1A6250537A4A24
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70H → P in AAA23952 (PubMed:2542273).Curated1
Sequence conflicti137 – 140SALP → LRVA in AAA23952 (PubMed:2542273).Curated4
Sequence conflicti365 – 366MH → ID in AAA23952 (PubMed:2542273).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04726 Genomic DNA Translation: AAA23952.1
U00096 Genomic DNA Translation: AAC74048.1
AP009048 Genomic DNA Translation: BAA35727.1
PIRiA64837 HJECD4
RefSeqiNP_415482.1, NC_000913.3
WP_001295354.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74048; AAC74048; b0962
BAA35727; BAA35727; BAA35727
GeneIDi946227
KEGGiecj:JW0945
eco:b0962
PATRICifig|1411691.4.peg.1312

Similar proteinsi

Entry informationi

Entry nameiHELD_ECOLI
AccessioniPrimary (citable) accession number: P15038
Secondary accession number(s): P77623
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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