UniProtKB - P15034 (AMPP_ECOLI)
Protein
Xaa-Pro aminopeptidase
Gene
pepP
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide. EC:3.4.11.9
Cofactori
Mn2+Note: Binds 2 manganese ions per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 261 | Manganese 2 | 1 | |
Metal bindingi | 272 | Manganese 1 | 1 | |
Metal bindingi | 272 | Manganese 2 | 1 | |
Metal bindingi | 355 | Manganese 1 | 1 | |
Metal bindingi | 384 | Manganese 1 | 1 | |
Metal bindingi | 407 | Manganese 1 | 1 | |
Metal bindingi | 407 | Manganese 2 | 1 |
GO - Molecular functioni
- aminopeptidase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- manganese ion binding Source: EcoCyc
- metalloaminopeptidase activity Source: InterPro
- metalloexopeptidase activity Source: EcoCyc
- peptidase activity Source: GO_Central
GO - Biological processi
- protein homotetramerization Source: EcoCyc
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10697-MONOMER MetaCyc:EG10697-MONOMER |
BRENDAi | 3.1.8.1, 2026 3.4.11.9, 2026 |
Protein family/group databases
MEROPSi | M24.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Xaa-Pro aminopeptidase (EC:3.4.11.9)Alternative name(s): Aminoacylproline aminopeptidase Aminopeptidase P II Short name: APP-II X-Pro aminopeptidase |
Gene namesi | Name:pepP Ordered Locus Names:b2908, JW2876 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000185075 | 2 – 441 | Xaa-Pro aminopeptidaseAdd BLAST | 440 |
Proteomic databases
jPOSTi | P15034 |
PaxDbi | P15034 |
PRIDEi | P15034 |
Interactioni
Subunit structurei
Homotetramer.
Binary interactionsi
Hide detailsGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259236, 50 interactors |
DIPi | DIP-10459N |
IntActi | P15034, 10 interactors |
STRINGi | 511145.b2908 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P15034 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P15034 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M24B family.Curated
Phylogenomic databases
eggNOGi | COG0006, Bacteria |
HOGENOMi | CLU_017266_1_0_6 |
InParanoidi | P15034 |
PhylomeDBi | P15034 |
Family and domain databases
Gene3Di | 3.40.350.10, 1 hit 3.90.230.10, 1 hit |
InterProi | View protein in InterPro IPR007865, Aminopep_P_N IPR029149, Creatin/AminoP/Spt16_NTD IPR036005, Creatinase/aminopeptidase-like IPR000994, Pept_M24 IPR001714, Pept_M24_MAP IPR001131, Peptidase_M24B_aminopep-P_CS |
Pfami | View protein in Pfam PF05195, AMP_N, 1 hit PF00557, Peptidase_M24, 1 hit |
PRINTSi | PR00599, MAPEPTIDASE |
SMARTi | View protein in SMART SM01011, AMP_N, 1 hit |
SUPFAMi | SSF53092, SSF53092, 1 hit SSF55920, SSF55920, 1 hit |
PROSITEi | View protein in PROSITE PS00491, PROLINE_PEPTIDASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P15034-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEISRQEFQ RRRQALVEQM QPGSAALIFA APEVTRSADS EYPYRQNSDF
60 70 80 90 100
WYFTGFNEPE AVLVLIKSDD THNHSVLFNR VRDLTAEIWF GRRLGQDAAP
110 120 130 140 150
EKLGVDRALA FSEINQQLYQ LLNGLDVVYH AQGEYAYADV IVNSALEKLR
160 170 180 190 200
KGSRQNLTAP ATMIDWRPVV HEMRLFKSPE EIAVLRRAGE ITAMAHTRAM
210 220 230 240 250
EKCRPGMFEY HLEGEIHHEF NRHGARYPSY NTIVGSGENG CILHYTENEC
260 270 280 290 300
EMRDGDLVLI DAGCEYKGYA GDITRTFPVN GKFTQAQREI YDIVLESLET
310 320 330 340 350
SLRLYRPGTS ILEVTGEVVR IMVSGLVKLG ILKGDVDELI AQNAHRPFFM
360 370 380 390 400
HGLSHWLGLD VHDVGVYGQD RSRILEPGMV LTVEPGLYIA PDAEVPEQYR
410 420 430 440
GIGIRIEDDI VITETGNENL TASVVKKPEE IEALMVAARK Q
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00398 Genomic DNA Translation: BAA00299.1 D90281 Genomic DNA Translation: BAA14325.1 U28377 Genomic DNA Translation: AAA69076.1 U00096 Genomic DNA Translation: AAC75946.1 AP009048 Genomic DNA Translation: BAE76973.1 |
PIRi | JX0067, DPECP |
RefSeqi | NP_417384.1, NC_000913.3 WP_001290136.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75946; AAC75946; b2908 BAE76973; BAE76973; BAE76973 |
GeneIDi | 57730283 947385 |
KEGGi | ecj:JW2876 eco:b2908 |
PATRICi | fig|1411691.4.peg.3824 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00398 Genomic DNA Translation: BAA00299.1 D90281 Genomic DNA Translation: BAA14325.1 U28377 Genomic DNA Translation: AAA69076.1 U00096 Genomic DNA Translation: AAC75946.1 AP009048 Genomic DNA Translation: BAE76973.1 |
PIRi | JX0067, DPECP |
RefSeqi | NP_417384.1, NC_000913.3 WP_001290136.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A16 | X-ray | 2.30 | A | 2-441 | [»] | |
1JAW | X-ray | 2.70 | A | 2-441 | [»] | |
1M35 | X-ray | 2.40 | A/B/C/D/E/F | 2-441 | [»] | |
1N51 | X-ray | 2.30 | A | 2-441 | [»] | |
1W2M | X-ray | 2.40 | A/B/C/D/E/F | 2-441 | [»] | |
1W7V | X-ray | 2.00 | A/B/C/D | 2-441 | [»] | |
1WBQ | X-ray | 2.30 | A/B/C/D | 2-441 | [»] | |
1WL6 | X-ray | 2.00 | A | 2-441 | [»] | |
1WL9 | X-ray | 1.90 | A | 2-441 | [»] | |
1WLR | X-ray | 2.10 | A | 2-441 | [»] | |
2BH3 | X-ray | 2.40 | A | 2-441 | [»] | |
2BHA | X-ray | 2.40 | A | 2-441 | [»] | |
2BHB | X-ray | 2.41 | A | 2-441 | [»] | |
2BHC | X-ray | 2.40 | A | 2-441 | [»] | |
2BHD | X-ray | 2.50 | A | 2-441 | [»] | |
2BN7 | X-ray | 2.40 | A | 2-441 | [»] | |
2BWS | X-ray | 1.75 | A | 2-441 | [»] | |
2BWT | X-ray | 2.90 | A | 2-441 | [»] | |
2BWU | X-ray | 2.20 | A | 2-441 | [»] | |
2BWV | X-ray | 1.70 | A | 2-441 | [»] | |
2BWW | X-ray | 2.61 | A | 2-441 | [»] | |
2BWX | X-ray | 1.70 | A | 2-441 | [»] | |
2BWY | X-ray | 2.40 | A | 2-441 | [»] | |
2V3X | X-ray | 1.70 | A | 2-441 | [»] | |
2V3Y | X-ray | 1.60 | A | 2-441 | [»] | |
2V3Z | X-ray | 1.56 | A | 2-441 | [»] | |
SMRi | P15034 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259236, 50 interactors |
DIPi | DIP-10459N |
IntActi | P15034, 10 interactors |
STRINGi | 511145.b2908 |
Chemistry databases
DrugBanki | DB04092, Apstatin |
Protein family/group databases
MEROPSi | M24.004 |
Proteomic databases
jPOSTi | P15034 |
PaxDbi | P15034 |
PRIDEi | P15034 |
Genome annotation databases
EnsemblBacteriai | AAC75946; AAC75946; b2908 BAE76973; BAE76973; BAE76973 |
GeneIDi | 57730283 947385 |
KEGGi | ecj:JW2876 eco:b2908 |
PATRICi | fig|1411691.4.peg.3824 |
Organism-specific databases
EchoBASEi | EB0691 |
Phylogenomic databases
eggNOGi | COG0006, Bacteria |
HOGENOMi | CLU_017266_1_0_6 |
InParanoidi | P15034 |
PhylomeDBi | P15034 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10697-MONOMER MetaCyc:EG10697-MONOMER |
BRENDAi | 3.1.8.1, 2026 3.4.11.9, 2026 |
Miscellaneous databases
EvolutionaryTracei | P15034 |
PROi | PR:P15034 |
Family and domain databases
Gene3Di | 3.40.350.10, 1 hit 3.90.230.10, 1 hit |
InterProi | View protein in InterPro IPR007865, Aminopep_P_N IPR029149, Creatin/AminoP/Spt16_NTD IPR036005, Creatinase/aminopeptidase-like IPR000994, Pept_M24 IPR001714, Pept_M24_MAP IPR001131, Peptidase_M24B_aminopep-P_CS |
Pfami | View protein in Pfam PF05195, AMP_N, 1 hit PF00557, Peptidase_M24, 1 hit |
PRINTSi | PR00599, MAPEPTIDASE |
SMARTi | View protein in SMART SM01011, AMP_N, 1 hit |
SUPFAMi | SSF53092, SSF53092, 1 hit SSF55920, SSF55920, 1 hit |
PROSITEi | View protein in PROSITE PS00491, PROLINE_PEPTIDASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPP_ECOLI | |
Accessioni | P15034Primary (citable) accession number: P15034 Secondary accession number(s): Q2M9T3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families