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Protein

Exodeoxyribonuclease 8

Gene

recE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is involved in the RecE pathway of recombination. Catalyzes the degradation of double-stranded DNA. Acts progressively in a 5' to 3' direction, releasing 5'-phosphomononucleotides. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA.

GO - Molecular functioni

  • double-stranded DNA 5'-3' exodeoxyribonuclease activity Source: EcoCyc
  • identical protein binding Source: IntAct

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BioCyciEcoCyc:EG10827-MONOMER
MetaCyc:EG10827-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Exodeoxyribonuclease 8 (EC:3.1.11.-)
Alternative name(s):
Exodeoxyribonuclease VIII
Short name:
EXO VIII
Gene namesi
Name:recE
Ordered Locus Names:b1350, JW1344
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10827 recE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972271 – 866Exodeoxyribonuclease 8Add BLAST866

Proteomic databases

PaxDbiP15032
PRIDEiP15032

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-553304,EBI-553304

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4260163, 103 interactors
DIPiDIP-10652N
IntActiP15032, 11 interactors
STRINGi316385.ECDH10B_1471

Structurei

Secondary structure

1866
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP15032
SMRiP15032
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15032

Family & Domainsi

Phylogenomic databases

eggNOGiENOG41076J9 Bacteria
ENOG410XPW2 LUCA
HOGENOMiHOG000120165
KOiK10906

Family and domain databases

InterProiView protein in InterPro
IPR010584 ExoDNase_VIII
IPR024432 Put_RecE_PDDEXK-like_dom
PfamiView protein in Pfam
PF12684 DUF3799, 1 hit
PF06630 Exonuc_VIII, 1 hit

Sequencei

Sequence statusi: Complete.

P15032-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTKPLFLLR KAKKSSGEPD VVLWASNDFE STCATLDYLI VKSGKKLSSY
60 70 80 90 100
FKAVATNFPV VNDLPAEGEI DFTWSERYQL SKDSMTWELK PGAAPDNAHY
110 120 130 140 150
QGNTNVNGED MTEIEENMLL PISGQELPIR WLAQHGSEKP VTHVSRDGLQ
160 170 180 190 200
ALHIARAEEL PAVTALAVSH KTSLLDPLEI RELHKLVRDT DKVFPNPGNS
210 220 230 240 250
NLGLITAFFE AYLNADYTDR GLLTKEWMKG NRVSHITRTA SGANAGGGNL
260 270 280 290 300
TDRGEGFVHD LTSLARDVAT GVLARSMDLD IYNLHPAHAK RIEEIIAENK
310 320 330 340 350
PPFSVFRDKF ITMPGGLDYS RAIVVASVKE APIGIEVIPA HVTEYLNKVL
360 370 380 390 400
TETDHANPDP EIVDIACGRS SAPMPQRVTE EGKQDDEEKP QPSGTTAVEQ
410 420 430 440 450
GEAETMEPDA TEHHQDTQPL DAQSQVNSVD AKYQELRAEL HEARKNIPSK
460 470 480 490 500
NPVDDDKLLA ASRGEFVDGI SDPNDPKWVK GIQTRDCVYQ NQPETEKTSP
510 520 530 540 550
DMNQPEPVVQ QEPEIACNAC GQTGGDNCPD CGAVMGDATY QETFDEESQV
560 570 580 590 600
EAKENDPEEM EGAEHPHNEN AGSDPHRDCS DETGEVADPV IVEDIEPGIY
610 620 630 640 650
YGISNENYHA GPGISKSQLD DIADTPALYL WRKNAPVDTT KTKTLDLGTA
660 670 680 690 700
FHCRVLEPEE FSNRFIVAPE FNRRTNAGKE EEKAFLMECA STGKTVITAE
710 720 730 740 750
EGRKIELMYQ SVMALPLGQW LVESAGHAES SIYWEDPETG ILCRCRPDKI
760 770 780 790 800
IPEFHWIMDV KTTADIQRFK TAYYDYRYHV QDAFYSDGYE AQFGVQPTFV
810 820 830 840 850
FLVASTTIEC GRYPVEIFMM GEEAKLAGQQ EYHRNLRTLS DCLNTDEWPA
860
IKTLSLPRWA KEYAND
Length:866
Mass (Da):96,368
Last modified:November 1, 1997 - v3
Checksum:i6133E2FDDB12A538
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti366A → G in AAA03212 (PubMed:2649487).Curated1
Sequence conflicti691 – 692ST → TA (PubMed:8244937).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74432.1
AP009048 Genomic DNA Translation: BAA14950.1
M24905 Unassigned DNA Translation: AAA03212.1
X55956 Genomic DNA Translation: CAA39419.1 Sequence problems.
X55956 Genomic DNA Translation: CAA39420.1 Sequence problems.
X55956 Genomic DNA Translation: CAA39421.1 Sequence problems.
X55956 Genomic DNA Translation: CAA39422.1 Sequence problems.
X55956 Genomic DNA Translation: CAA39423.1 Sequence problems.
L23927 Unassigned DNA Translation: AAA16178.1
PIRiA64885 NCECX8
RefSeqiNP_415866.1, NC_000913.3
WP_000105143.1, NZ_CP014272.1

Genome annotation databases

EnsemblBacteriaiAAC74432; AAC74432; b1350
BAA14950; BAA14950; BAA14950
GeneIDi945918
KEGGiecj:JW1344
eco:b1350
PATRICifig|1411691.4.peg.927

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74432.1
AP009048 Genomic DNA Translation: BAA14950.1
M24905 Unassigned DNA Translation: AAA03212.1
X55956 Genomic DNA Translation: CAA39419.1 Sequence problems.
X55956 Genomic DNA Translation: CAA39420.1 Sequence problems.
X55956 Genomic DNA Translation: CAA39421.1 Sequence problems.
X55956 Genomic DNA Translation: CAA39422.1 Sequence problems.
X55956 Genomic DNA Translation: CAA39423.1 Sequence problems.
L23927 Unassigned DNA Translation: AAA16178.1
PIRiA64885 NCECX8
RefSeqiNP_415866.1, NC_000913.3
WP_000105143.1, NZ_CP014272.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H4RX-ray2.80A606-866[»]
ProteinModelPortaliP15032
SMRiP15032
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260163, 103 interactors
DIPiDIP-10652N
IntActiP15032, 11 interactors
STRINGi316385.ECDH10B_1471

Proteomic databases

PaxDbiP15032
PRIDEiP15032

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74432; AAC74432; b1350
BAA14950; BAA14950; BAA14950
GeneIDi945918
KEGGiecj:JW1344
eco:b1350
PATRICifig|1411691.4.peg.927

Organism-specific databases

EchoBASEiEB0820
EcoGeneiEG10827 recE

Phylogenomic databases

eggNOGiENOG41076J9 Bacteria
ENOG410XPW2 LUCA
HOGENOMiHOG000120165
KOiK10906

Enzyme and pathway databases

BioCyciEcoCyc:EG10827-MONOMER
MetaCyc:EG10827-MONOMER

Miscellaneous databases

EvolutionaryTraceiP15032
PROiPR:P15032

Family and domain databases

InterProiView protein in InterPro
IPR010584 ExoDNase_VIII
IPR024432 Put_RecE_PDDEXK-like_dom
PfamiView protein in Pfam
PF12684 DUF3799, 1 hit
PF06630 Exonuc_VIII, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRECE_ECOLI
AccessioniPrimary (citable) accession number: P15032
Secondary accession number(s): P76848, P77807
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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