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Protein

Leukemia inhibitory factor

Gene

LIF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • growth factor activity Source: BHF-UCL
  • leukemia inhibitory factor receptor binding Source: MGI
  • signaling receptor binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionCytokine, Growth factor

Enzyme and pathway databases

ReactomeiR-HSA-6783783 Interleukin-10 signaling
R-HSA-6785807 Interleukin-4 and 13 signaling
R-HSA-6788467 IL-6-type cytokine receptor ligand interactions
SignaLinkiP15018
SIGNORiP15018

Names & Taxonomyi

Protein namesi
Recommended name:
Leukemia inhibitory factor
Short name:
LIF
Alternative name(s):
Differentiation-stimulating factor
Short name:
D factor
Melanoma-derived LPL inhibitor
Short name:
MLPLI
INN: Emfilermin
Gene namesi
Name:LIF
Synonyms:HILDA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000128342.4
HGNCiHGNC:6596 LIF
MIMi159540 gene
neXtProtiNX_P15018

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

In phase II clinical trial. The drug is being developed by Amrad to assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART).

Organism-specific databases

DisGeNETi3976
OpenTargetsiENSG00000128342
PharmGKBiPA30370

Polymorphism and mutation databases

BioMutaiLIF
DMDMi126279

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000001771523 – 202Leukemia inhibitory factorAdd BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi31N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi34 ↔ 1561 Publication
Disulfide bondi40 ↔ 1531 Publication
Glycosylationi56N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi82 ↔ 1851 Publication
Glycosylationi85N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi95N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi118N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi138N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP15018
PaxDbiP15018
PeptideAtlasiP15018
PRIDEiP15018
ProteomicsDBi53100

PTM databases

iPTMnetiP15018

Miscellaneous databases

PMAP-CutDBiP15018

Expressioni

Gene expression databases

BgeeiENSG00000128342
CleanExiHS_LIF
GenevisibleiP15018 HS

Organism-specific databases

HPAiCAB025985
HPA018844

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
IL6STP401892EBI-1037189,EBI-1030834

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • growth factor activity Source: BHF-UCL
  • leukemia inhibitory factor receptor binding Source: MGI
  • signaling receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi110164, 1 interactor
CORUMiP15018
DIPiDIP-5769N
IntActiP15018, 7 interactors
MINTiP15018
STRINGi9606.ENSP00000249075

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi44 – 70Combined sources27
Turni72 – 77Combined sources6
Helixi78 – 81Combined sources4
Beta strandi82 – 84Combined sources3
Beta strandi94 – 96Combined sources3
Helixi98 – 126Combined sources29
Helixi131 – 157Combined sources27
Helixi177 – 199Combined sources23

3D structure databases

ProteinModelPortaliP15018
SMRiP15018
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15018

Family & Domainsi

Sequence similaritiesi

Belongs to the LIF/OSM family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410ITBQ Eukaryota
ENOG410YVPP LUCA
GeneTreeiENSGT00390000000059
HOGENOMiHOG000059647
HOVERGENiHBG006267
InParanoidiP15018
KOiK05419
OMAiKYHVAHV
OrthoDBiEOG091G0HWD
PhylomeDBiP15018
TreeFamiTF336245

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR003624 Leukemia_IF
IPR001581 Leukemia_IF/oncostatin
IPR019827 Leukemia_IF/oncostatin_CS
PANTHERiPTHR10633 PTHR10633, 1 hit
PfamiView protein in Pfam
PF01291 LIF_OSM, 1 hit
PRINTSiPR01883 LEUKAEMIAIF
SMARTiView protein in SMART
SM00080 LIF_OSM, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit
PROSITEiView protein in PROSITE
PS00590 LIF_OSM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P15018-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVLAAGVVP LLLVLHWKHG AGSPLPITPV NATCAIRHPC HNNLMNQIRS
60 70 80 90 100
QLAQLNGSAN ALFILYYTAQ GEPFPNNLDK LCGPNVTDFP PFHANGTEKA
110 120 130 140 150
KLVELYRIVV YLGTSLGNIT RDQKILNPSA LSLHSKLNAT ADILRGLLSN
160 170 180 190 200
VLCRLCSKYH VGHVDVTYGP DTSGKDVFQK KKLGCQLLGK YKQIIAVLAQ

AF
Length:202
Mass (Da):22,008
Last modified:April 1, 1990 - v1
Checksum:i634CD10BFF67A217
GO
Isoform 2 (identifier: P15018-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     7-88: GVVPLLLVLH...DKLCGPNVTD → VHSPGGAVPQ...RHRRHPARPP
     89-202: Missing.

Note: No experimental confirmation available.
Show »
Length:88
Mass (Da):9,563
Checksum:iC27010E1B89B18E5
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0455517 – 88GVVPL…PNVTD → VHSPGGAVPQQPGQAMWPQR DGLPALPRQRHGEGQAGGAV PHSRVPWHLPGQHHPGPEDP QPQCPQPPQQAQRHRRHPAR PP in isoform 2. 1 PublicationAdd BLAST82
Alternative sequenceiVSP_04555289 – 202Missing in isoform 2. 1 PublicationAdd BLAST114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13967 mRNA Translation: CAA32147.1
M27053, M27052 Genomic DNA Translation: AAA53188.1
AC004264 Genomic DNA Translation: AAC05174.1
M63420 Genomic DNA Translation: AAA51699.1
CN409468 mRNA No translation available.
AK315310 mRNA Translation: BAG37714.1
CH471095 Genomic DNA Translation: EAW59863.1
BC069540 mRNA Translation: AAH69540.1
BC093733 mRNA Translation: AAH93733.1
BC093735 mRNA Translation: AAH93735.1
J03261 Genomic DNA Translation: AAA59517.1
CCDSiCCDS13872.1 [P15018-1]
CCDS58799.1 [P15018-2]
PIRiB36282
RefSeqiNP_001244064.1, NM_001257135.1 [P15018-2]
NP_002300.1, NM_002309.4 [P15018-1]
UniGeneiHs.2250

Genome annotation databases

EnsembliENST00000249075; ENSP00000249075; ENSG00000128342 [P15018-1]
ENST00000403987; ENSP00000384450; ENSG00000128342 [P15018-2]
GeneIDi3976
KEGGihsa:3976
UCSCiuc003agz.3 human [P15018-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiLIF_HUMAN
AccessioniPrimary (citable) accession number: P15018
Secondary accession number(s): B2RCW7, B5MC23, Q52LZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 18, 2018
This is version 188 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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