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Entry version 167 (13 Nov 2019)
Sequence version 3 (15 Dec 1998)
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Protein

5-methylcytosine-specific restriction enzyme B

Gene

mcrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi201 – 208GTPSequence analysis8
Nucleotide bindingi300 – 303GTPSequence analysis4
Nucleotide bindingi333 – 336GTPSequence analysis4

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG10574-MONOMER
ECOL316407:JW5871-MONOMER
MetaCyc:EG10574-MONOMER

Protein family/group databases

Restriction enzymes and methylases database

More...
REBASEi
13377 EcoW3110McrBCP
203811 Keu1446McrBCP
2865 EcoKMcrBC

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
5-methylcytosine-specific restriction enzyme B (EC:3.1.21.-)
Alternative name(s):
EcoKMcrBC
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:mcrB
Synonyms:rglB
Ordered Locus Names:b4346, JW5871
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000303471 – 4595-methylcytosine-specific restriction enzyme BAdd BLAST459

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P15005

PRoteomics IDEntifications database

More...
PRIDEi
P15005

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
P318063EBI-552513,EBI-554188

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4262765, 164 interactors
853362, 2 interactors

Database of interacting proteins

More...
DIPi
DIP-10168N

Protein interaction database and analysis system

More...
IntActi
P15005, 3 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b4346

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1459
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P15005

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105FBQ Bacteria
COG1401 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000081315

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P15005

KEGG Orthology (KO)

More...
KOi
K07452

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P15005

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003593 AAA+_ATPase
IPR011704 ATPase_dyneun-rel_AAA
IPR021961 DUF3578
IPR027417 P-loop_NTPase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07728 AAA_5, 1 hit
PF12102 DUF3578, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00382 AAA, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540 SSF52540, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative initiation. AlignAdd to basket
Isoform 51 kDa (identifier: P15005-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MESIQPWIEK FIKQAQQQRS QSTKDYPTSY RNLRVKLSFG YGNFTSIPWF
60 70 80 90 100
AFLGEGQEAS NGIYPVILYY KDFDELVLAY GISDTNEPHA QWQFSSDIPK
110 120 130 140 150
TIAEYFQATS GVYPKKYGQS YYACSQKVSQ GIDYTRFASM LDNIINDYKL
160 170 180 190 200
IFNSGKSVIP PMSKTESYCL EDALNDLFIP ETTIETILKR LTIKKNIILQ
210 220 230 240 250
GPPGVGKTFV ARRLAYLLTG EKAPQRVNMV QFHQSYSYED FIQGYRPNGV
260 270 280 290 300
GFRRKDGIFY NFCQQAKEQP EKKYIFIIDE INRANLSKVF GEVMMLMEHD
310 320 330 340 350
KRGENWSVPL TYSENDEERF YVPENVYIIG LMNTADRSLA VVDYALRRRF
360 370 380 390 400
SFIDIEPGFD TPQFRNFLLN KKAEPSFVES LCQKMNELNQ EISKEATILG
410 420 430 440 450
KGFRIGHSYF CCGLEDGTSP DTQWLNEIVM TDIAPLLEEY FFDDPYKQQK

WTNKLLGDS
Length:459
Mass (Da):53,157
Last modified:December 15, 1998 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC8167978B0D720CA
GO
Isoform 33 kDa (identifier: P15005-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Show »
Length:298
Mass (Da):34,660
Checksum:iB05DE88C3CAB9321
GO

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA24145 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAA97243 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti186 – 212TILKR…TFVAR → SDTQTINHQKKYYPPGAARR WKNLCCT in AAA24142 (PubMed:2649480).CuratedAdd BLAST27
Sequence conflicti224 – 232PQRVNMVQF → RTKYGSV in AAA24142 (PubMed:2649480).Curated9
Sequence conflicti245 – 259YRPNG…KDGIF → IVRMASASVKTHI (PubMed:2649480).CuratedAdd BLAST15

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0188681 – 161Missing in isoform 33 kDa. CuratedAdd BLAST161

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M58752 Genomic DNA Translation: AAA24145.1 Different initiation.
M24927 Genomic DNA Translation: AAA24142.1
U14003 Genomic DNA Translation: AAA97243.1 Different initiation.
U00096 Genomic DNA Translation: AAC77302.2
AP009048 Genomic DNA Translation: BAE78336.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A36708 XYECMB

NCBI Reference Sequences

More...
RefSeqi
NP_418766.4, NC_000913.3
WP_000443951.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC77302; AAC77302; b4346
BAE78336; BAE78336; BAE78336

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
949122

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW5871
eco:b4346

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|511145.12.peg.4492

Keywords - Coding sequence diversityi

Alternative initiation

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58752 Genomic DNA Translation: AAA24145.1 Different initiation.
M24927 Genomic DNA Translation: AAA24142.1
U14003 Genomic DNA Translation: AAA97243.1 Different initiation.
U00096 Genomic DNA Translation: AAC77302.2
AP009048 Genomic DNA Translation: BAE78336.1
PIRiA36708 XYECMB
RefSeqiNP_418766.4, NC_000913.3
WP_000443951.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SSCX-ray2.10A/B1-161[»]
3SSDX-ray2.20A/B1-161[»]
3SSEX-ray2.70A/B1-161[»]
6GCDX-ray1.80A/B1-161[»]
6GCEX-ray1.60A/B1-161[»]
6GCFX-ray1.55A/B1-161[»]
6HZ4electron microscopy3.60A/B/C/D/E/F162-459[»]
6HZ5electron microscopy4.20A/B/C/D/E/F/G/H/I/J/K/L162-459[»]
6HZ6electron microscopy4.30A/B/C/D/E/F/G/H/I/J/K/L162-459[»]
6HZ7electron microscopy4.30A/B/C/D/E/F/G/H/I/J/K/L162-459[»]
6HZ8electron microscopy4.30A/B/C/D/E/F/G/H/I/J/K/L162-459[»]
6HZ9electron microscopy4.80A/B/C/D/E/F/G/H/I/J/K/L162-459[»]
SMRiP15005
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi4262765, 164 interactors
853362, 2 interactors
DIPiDIP-10168N
IntActiP15005, 3 interactors
STRINGi511145.b4346

Protein family/group databases

REBASEi13377 EcoW3110McrBCP
203811 Keu1446McrBCP
2865 EcoKMcrBC

Proteomic databases

PaxDbiP15005
PRIDEiP15005

Genome annotation databases

EnsemblBacteriaiAAC77302; AAC77302; b4346
BAE78336; BAE78336; BAE78336
GeneIDi949122
KEGGiecj:JW5871
eco:b4346
PATRICifig|511145.12.peg.4492

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0569

Phylogenomic databases

eggNOGiENOG4105FBQ Bacteria
COG1401 LUCA
HOGENOMiHOG000081315
InParanoidiP15005
KOiK07452
PhylomeDBiP15005

Enzyme and pathway databases

BioCyciEcoCyc:EG10574-MONOMER
ECOL316407:JW5871-MONOMER
MetaCyc:EG10574-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P15005

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011704 ATPase_dyneun-rel_AAA
IPR021961 DUF3578
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF07728 AAA_5, 1 hit
PF12102 DUF3578, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMCRB_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P15005
Secondary accession number(s): Q2M5X0
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 15, 1998
Last modified: November 13, 2019
This is version 167 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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