UniProtKB - P15005 (MCRB_ECOLI)
Protein
5-methylcytosine-specific restriction enzyme B
Gene
mcrB
Organism
Escherichia coli (strain K12)
Status
Functioni
Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 201 – 208 | GTPSequence analysis | 8 | |
Nucleotide bindingi | 300 – 303 | GTPSequence analysis | 4 | |
Nucleotide bindingi | 333 – 336 | GTPSequence analysis | 4 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: InterPro
- DNA binding Source: EcoliWiki
- GTPase activity Source: EcoliWiki
- GTP binding Source: UniProtKB-KW
- restriction endodeoxyribonuclease activity Source: EcoliWiki
GO - Biological processi
- DNA catabolic process Source: EcoliWiki
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Endonuclease, Hydrolase, Nuclease |
Biological process | Restriction system |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10574-MONOMER ECOL316407:JW5871-MONOMER MetaCyc:EG10574-MONOMER |
Protein family/group databases
REBASEi | 13377 EcoW3110McrBCP 203811 Keu1446McrBCP 2865 EcoKMcrBC |
Names & Taxonomyi
Protein namesi | Recommended name: 5-methylcytosine-specific restriction enzyme B (EC:3.1.21.-)Alternative name(s): EcoKMcrBC |
Gene namesi | Name:mcrB Synonyms:rglB Ordered Locus Names:b4346, JW5871 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000030347 | 1 – 459 | 5-methylcytosine-specific restriction enzyme BAdd BLAST | 459 |
Proteomic databases
PaxDbi | P15005 |
PRIDEi | P15005 |
Interactioni
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
P31806 | 3 | EBI-552513,EBI-554188 |
Protein-protein interaction databases
BioGridi | 4262765, 164 interactors 853362, 2 interactors |
DIPi | DIP-10168N |
IntActi | P15005, 3 interactors |
STRINGi | 511145.b4346 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P15005 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG4105FBQ Bacteria COG1401 LUCA |
HOGENOMi | HOG000081315 |
InParanoidi | P15005 |
KOi | K07452 |
PhylomeDBi | P15005 |
Family and domain databases
InterProi | View protein in InterPro IPR003593 AAA+_ATPase IPR011704 ATPase_dyneun-rel_AAA IPR021961 DUF3578 IPR027417 P-loop_NTPase |
Pfami | View protein in Pfam PF07728 AAA_5, 1 hit PF12102 DUF3578, 1 hit |
SMARTi | View protein in SMART SM00382 AAA, 1 hit |
SUPFAMi | SSF52540 SSF52540, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketIsoform 51 kDa (identifier: P15005-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MESIQPWIEK FIKQAQQQRS QSTKDYPTSY RNLRVKLSFG YGNFTSIPWF
60 70 80 90 100
AFLGEGQEAS NGIYPVILYY KDFDELVLAY GISDTNEPHA QWQFSSDIPK
110 120 130 140 150
TIAEYFQATS GVYPKKYGQS YYACSQKVSQ GIDYTRFASM LDNIINDYKL
160 170 180 190 200
IFNSGKSVIP PMSKTESYCL EDALNDLFIP ETTIETILKR LTIKKNIILQ
210 220 230 240 250
GPPGVGKTFV ARRLAYLLTG EKAPQRVNMV QFHQSYSYED FIQGYRPNGV
260 270 280 290 300
GFRRKDGIFY NFCQQAKEQP EKKYIFIIDE INRANLSKVF GEVMMLMEHD
310 320 330 340 350
KRGENWSVPL TYSENDEERF YVPENVYIIG LMNTADRSLA VVDYALRRRF
360 370 380 390 400
SFIDIEPGFD TPQFRNFLLN KKAEPSFVES LCQKMNELNQ EISKEATILG
410 420 430 440 450
KGFRIGHSYF CCGLEDGTSP DTQWLNEIVM TDIAPLLEEY FFDDPYKQQK
WTNKLLGDS
Isoform 33 kDa (identifier: P15005-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-161: Missing.
Sequence cautioni
The sequence AAA24145 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAA97243 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 186 – 212 | TILKR…TFVAR → SDTQTINHQKKYYPPGAARR WKNLCCT in AAA24142 (PubMed:2649480).CuratedAdd BLAST | 27 | |
Sequence conflicti | 224 – 232 | PQRVNMVQF → RTKYGSV in AAA24142 (PubMed:2649480).Curated | 9 | |
Sequence conflicti | 245 – 259 | YRPNG…KDGIF → IVRMASASVKTHI (PubMed:2649480).CuratedAdd BLAST | 15 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018868 | 1 – 161 | Missing in isoform 33 kDa. CuratedAdd BLAST | 161 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58752 Genomic DNA Translation: AAA24145.1 Different initiation. M24927 Genomic DNA Translation: AAA24142.1 U14003 Genomic DNA Translation: AAA97243.1 Different initiation. U00096 Genomic DNA Translation: AAC77302.2 AP009048 Genomic DNA Translation: BAE78336.1 |
PIRi | A36708 XYECMB |
RefSeqi | NP_418766.4, NC_000913.3 WP_000443951.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77302; AAC77302; b4346 BAE78336; BAE78336; BAE78336 |
GeneIDi | 949122 |
KEGGi | ecj:JW5871 eco:b4346 |
PATRICi | fig|511145.12.peg.4492 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58752 Genomic DNA Translation: AAA24145.1 Different initiation. M24927 Genomic DNA Translation: AAA24142.1 U14003 Genomic DNA Translation: AAA97243.1 Different initiation. U00096 Genomic DNA Translation: AAC77302.2 AP009048 Genomic DNA Translation: BAE78336.1 |
PIRi | A36708 XYECMB |
RefSeqi | NP_418766.4, NC_000913.3 WP_000443951.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3SSC | X-ray | 2.10 | A/B | 1-161 | [»] | |
3SSD | X-ray | 2.20 | A/B | 1-161 | [»] | |
3SSE | X-ray | 2.70 | A/B | 1-161 | [»] | |
6GCD | X-ray | 1.80 | A/B | 1-161 | [»] | |
6GCE | X-ray | 1.60 | A/B | 1-161 | [»] | |
6GCF | X-ray | 1.55 | A/B | 1-161 | [»] | |
6HZ4 | electron microscopy | 3.60 | A/B/C/D/E/F | 162-459 | [»] | |
6HZ5 | electron microscopy | 4.20 | A/B/C/D/E/F/G/H/I/J/K/L | 162-459 | [»] | |
6HZ6 | electron microscopy | 4.30 | A/B/C/D/E/F/G/H/I/J/K/L | 162-459 | [»] | |
6HZ7 | electron microscopy | 4.30 | A/B/C/D/E/F/G/H/I/J/K/L | 162-459 | [»] | |
6HZ8 | electron microscopy | 4.30 | A/B/C/D/E/F/G/H/I/J/K/L | 162-459 | [»] | |
6HZ9 | electron microscopy | 4.80 | A/B/C/D/E/F/G/H/I/J/K/L | 162-459 | [»] | |
SMRi | P15005 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 4262765, 164 interactors 853362, 2 interactors |
DIPi | DIP-10168N |
IntActi | P15005, 3 interactors |
STRINGi | 511145.b4346 |
Protein family/group databases
REBASEi | 13377 EcoW3110McrBCP 203811 Keu1446McrBCP 2865 EcoKMcrBC |
Proteomic databases
PaxDbi | P15005 |
PRIDEi | P15005 |
Genome annotation databases
EnsemblBacteriai | AAC77302; AAC77302; b4346 BAE78336; BAE78336; BAE78336 |
GeneIDi | 949122 |
KEGGi | ecj:JW5871 eco:b4346 |
PATRICi | fig|511145.12.peg.4492 |
Organism-specific databases
EchoBASEi | EB0569 |
Phylogenomic databases
eggNOGi | ENOG4105FBQ Bacteria COG1401 LUCA |
HOGENOMi | HOG000081315 |
InParanoidi | P15005 |
KOi | K07452 |
PhylomeDBi | P15005 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10574-MONOMER ECOL316407:JW5871-MONOMER MetaCyc:EG10574-MONOMER |
Miscellaneous databases
PROi | PR:P15005 |
Family and domain databases
InterProi | View protein in InterPro IPR003593 AAA+_ATPase IPR011704 ATPase_dyneun-rel_AAA IPR021961 DUF3578 IPR027417 P-loop_NTPase |
Pfami | View protein in Pfam PF07728 AAA_5, 1 hit PF12102 DUF3578, 1 hit |
SMARTi | View protein in SMART SM00382 AAA, 1 hit |
SUPFAMi | SSF52540 SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCRB_ECOLI | |
Accessioni | P15005Primary (citable) accession number: P15005 Secondary accession number(s): Q2M5X0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | December 15, 1998 | |
Last modified: | November 13, 2019 | |
This is version 167 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene - PDB cross-references
Index of Protein Data Bank (PDB) cross-references