UniProtKB - P14996 (VP1_POVBA)
Major capsid protein VP1
Functioni
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with gangliosides GT1b and GD1b containing terminal alpha2-8-linked sialic acids on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA in the nucleus, and participates in rearranging nucleosomes around the viral DNA.
By similarity1 PublicationGO - Molecular functioni
- structural molecule activity Source: InterPro
GO - Biological processi
- caveolin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Major capsid protein VP1Alternative name(s): Major structural protein VP1 |
Organismi | BK polyomavirus (strain AS) (BKPyV) |
Taxonomic identifieri | 10631 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Papovaviricetes › Sepolyvirales › Polyomaviridae › Betapolyomavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion
- Host nucleus By similarity
Keywords - Cellular componenti
Capsid protein, Host nucleus, T=7 icosahedral capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000115017 | 1 – 362 | Major capsid protein VP1Add BLAST | 362 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 10 | Interchain (with C-10)By similarity | ||
Disulfide bondi | 105 | Interchain (with C-105)By similarity | ||
Modified residuei | 338 | Phosphothreonine; by hostBy similarity | 1 |
Keywords - PTMi
Disulfide bond, PhosphoproteinInteractioni
Subunit structurei
Homomultimer; disulfide-linked. The virus capsid is composed of 72 icosahedral units, each one composed of five disulfide-linked copies of VP1.
Interacts with minor capsid proteins VP2 and VP3.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 21 | DisorderedSequence analysisAdd BLAST | 21 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 5 – 19 | Bipartite nuclear localization signalBy similarityAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Family and domain databases
Gene3Di | 2.60.175.10, 1 hit |
InterProi | View protein in InterPro IPR000662, Capsid_VP1_Polyomavir IPR011222, dsDNA_vir_gr_I_capsid IPR036931, Polyomavir_VP1_sf |
Pfami | View protein in Pfam PF00718, Polyoma_coat, 1 hit |
PIRSFi | PIRSF003376, Capsid_VP1_Polyomavir, 1 hit |
SUPFAMi | SSF88648, SSF88648, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPTKRKGEC PGAAPKKPKE PVQVPKLLIK GGVEVLEVKT GVDAITEVEC
60 70 80 90 100
FLNPEMGDPD DNLRGYSQHL SAENAFESDS PDRKMLPCYS TARIPLPNLN
110 120 130 140 150
EDLTCGNLLM WEAVTVKTEV IGITSMLNLH AGSQKVHENG GGKPVQGSNF
160 170 180 190 200
HFFAVGGDPL EMQGVLMNYR TKYPQGTITP KNPTAQSQVM NTDHKAYLDK
210 220 230 240 250
NNAYPVECWI PDPSRNENTR YFGTYTGGEN VPPVLHVTNT ATTVLLDEQG
260 270 280 290 300
VGPLCKADSL YVSAADICGL FTNSSGTQQW RGLARYFKIR LRKRSVKNPY
310 320 330 340 350
PISFLLSDLI NRRTQKVDGQ PMYGMESQVE EVRVFDGTEQ LPGDPDMIRY
360
IDRQGQLQTK MV
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23122 Genomic DNA Translation: AAA46882.1 |
PIRi | D33278, VVVP1S |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23122 Genomic DNA Translation: AAA46882.1 |
PIRi | D33278, VVVP1S |
3D structure databases
SMRi | P14996 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 2.60.175.10, 1 hit |
InterProi | View protein in InterPro IPR000662, Capsid_VP1_Polyomavir IPR011222, dsDNA_vir_gr_I_capsid IPR036931, Polyomavir_VP1_sf |
Pfami | View protein in Pfam PF00718, Polyoma_coat, 1 hit |
PIRSFi | PIRSF003376, Capsid_VP1_Polyomavir, 1 hit |
SUPFAMi | SSF88648, SSF88648, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VP1_POVBA | |
Accessioni | P14996Primary (citable) accession number: P14996 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | June 2, 2021 | |
This is version 93 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |