UniProtKB - P14922 (CYC8_YEAST)
General transcriptional corepressor CYC8
CYC8
Functioni
Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex.
5 PublicationsMiscellaneous
GO - Molecular functioni
- chromatin DNA binding Source: GO_Central
- histone deacetylase binding Source: SGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- transcription coactivator activity Source: SGD
- transcription corepressor activity Source: SGD
GO - Biological processi
- chromatin remodeling Source: SGD
- hyperosmotic response Source: ComplexPortal
- negative regulation of single-species biofilm formation Source: SGD
- negative regulation of transcription, DNA-templated Source: ComplexPortal
- negative regulation of transcription by RNA polymerase II Source: SGD
- nucleosome positioning Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- regulation of gene expression Source: GO_Central
- regulation of response to DNA damage stimulus Source: SGD
- regulation of transcription by RNA polymerase II Source: SGD
Keywordsi
Molecular function | Prion, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-3214842, HDMs demethylate histones |
Names & Taxonomyi
Protein namesi | Recommended name: General transcriptional corepressor CYC8Alternative name(s): Glucose repression mediator protein CYC8 |
Gene namesi | Name:CYC8 Synonyms:CRT8, SSN6 Ordered Locus Names:YBR112C ORF Names:YBR0908 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000316, CYC8 |
VEuPathDBi | FungiDB:YBR112C |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: SGD
Other locations
- transcription repressor complex Source: SGD
Keywords - Cellular componenti
Amyloid, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106327 | 1 – 966 | General transcriptional corepressor CYC8Add BLAST | 966 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 429 | PhosphoserineCombined sources | 1 | |
Modified residuei | 475 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 710 | PhosphoserineCombined sources | 1 | |
Modified residuei | 741 | PhosphoserineCombined sources | 1 | |
Modified residuei | 768 | PhosphoserineCombined sources | 1 | |
Modified residuei | 815 | PhosphoserineCombined sources | 1 | |
Modified residuei | 817 | PhosphoserineCombined sources | 1 | |
Modified residuei | 866 | PhosphoserineCombined sources | 1 | |
Modified residuei | 943 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P14922 |
PaxDbi | P14922 |
PRIDEi | P14922 |
PTM databases
iPTMneti | P14922 |
Interactioni
Subunit structurei
Associates with TUP1 to form the CYC8-TUP1 (or TUP1-SSN6) corepressor complex that is composed of 4 copies of TUP1 and one copy of CYC8.
Interacts with MATALPHA2, CTI6, MIG1, TUP1, SUT1, RFX1, PGD1, HOS1, HOS2 AND RPD3.
10 PublicationsBinary interactionsi
P14922
With | #Exp. | IntAct |
---|---|---|
HOS2 [P53096] | 4 | EBI-18215,EBI-8475 |
PGD1 [P40356] | 3 | EBI-18215,EBI-13268 |
RFX1 [P48743] | 2 | EBI-18215,EBI-15036 |
RPD3 [P32561] | 6 | EBI-18215,EBI-15864 |
TUP1 [P16649] | 6 | EBI-18215,EBI-19654 |
GO - Molecular functioni
- histone deacetylase binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 32816, 176 interactors |
ComplexPortali | CPX-1663, CYP8-TUP1 corepressor complex |
DIPi | DIP-696N |
IntActi | P14922, 10 interactors |
MINTi | P14922 |
STRINGi | 4932.YBR112C |
Miscellaneous databases
RNActi | P14922, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 46 – 79 | TPR 1Add BLAST | 34 | |
Repeati | 80 – 113 | TPR 2Add BLAST | 34 | |
Repeati | 114 – 147 | TPR 3Add BLAST | 34 | |
Repeati | 150 – 183 | TPR 4Add BLAST | 34 | |
Repeati | 187 – 220 | TPR 5Add BLAST | 34 | |
Repeati | 224 – 257 | TPR 6Add BLAST | 34 | |
Repeati | 262 – 295 | TPR 7Add BLAST | 34 | |
Repeati | 296 – 329 | TPR 8Add BLAST | 34 | |
Repeati | 330 – 363 | TPR 9Add BLAST | 34 | |
Repeati | 364 – 398 | TPR 10Add BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 37 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 412 – 489 | DisorderedSequence analysisAdd BLAST | 78 | |
Regioni | 467 – 682 | Prion domain (PrD)Add BLAST | 216 | |
Regioni | 631 – 666 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 699 – 723 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 759 – 966 | DisorderedSequence analysisAdd BLAST | 208 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 412 – 426 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 440 – 489 | Polar residuesSequence analysisAdd BLAST | 50 | |
Compositional biasi | 759 – 787 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 812 – 860 | Polar residuesSequence analysisAdd BLAST | 49 | |
Compositional biasi | 922 – 941 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG1124, Eukaryota |
GeneTreei | ENSGT00940000168210 |
HOGENOMi | CLU_006762_3_0_1 |
InParanoidi | P14922 |
OMAi | YLGRVEM |
Family and domain databases
Gene3Di | 1.25.40.10, 3 hits |
InterProi | View protein in InterPro IPR011990, TPR-like_helical_dom_sf IPR001440, TPR_1 IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF00515, TPR_1, 1 hit |
SMARTi | View protein in SMART SM00028, TPR, 10 hits |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50005, TPR, 9 hits PS50293, TPR_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNPGGEQTIM EQPAQQQQQQ QQQQQQQQQQ AAVPQQPLDP LTQSTAETWL
60 70 80 90 100
SIASLAETLG DGDRAAMAYD ATLQFNPSSA KALTSLAHLY RSRDMFQRAA
110 120 130 140 150
ELYERALLVN PELSDVWATL GHCYLMLDDL QRAYNAYQQA LYHLSNPNVP
160 170 180 190 200
KLWHGIGILY DRYGSLDYAE EAFAKVLELD PHFEKANEIY FRLGIIYKHQ
210 220 230 240 250
GKWSQALECF RYILPQPPAP LQEWDIWFQL GSVLESMGEW QGAKEAYEHV
260 270 280 290 300
LAQNQHHAKV LQQLGCLYGM SNVQFYDPQK ALDYLLKSLE ADPSDATTWY
310 320 330 340 350
HLGRVHMIRT DYTAAYDAFQ QAVNRDSRNP IFWCSIGVLY YQISQYRDAL
360 370 380 390 400
DAYTRAIRLN PYISEVWYDL GTLYETCNNQ LSDALDAYKQ AARLDVNNVH
410 420 430 440 450
IRERLEALTK QLENPGNINK SNGAPTNASP APPPVILQPT LQPNDQGNPL
460 470 480 490 500
NTRISAQSAN ATASMVQQQH PAQQTPINSS ATMYSNGASP QLQAQAQAQA
510 520 530 540 550
QAQAQAQAQA QAQAQAQAQA QAQAQAQAQA QAQAQAHAQA QAQAQAQAQA
560 570 580 590 600
QAQAQAQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQLQP LPRQQLQQKG
610 620 630 640 650
VSVQMLNPQQ GQPYITQPTV IQAHQLQPFS TQAMEHPQSS QLPPQQQQLQ
660 670 680 690 700
SVQHPQQLQG QPQAQAPQPL IQHNVEQNVL PQKRYMEGAI HTLVDAAVSS
710 720 730 740 750
STHTENNTKS PRQPTHAIPT QAPATGITNA EPQVKKQKLN SPNSNINKLV
760 770 780 790 800
NTATSIEENA KSEVSNQSPA VVESNTNNTS QEEKPVKANS IPSVIGAQEP
810 820 830 840 850
PQEASPAEEA TKAASVSPST KPLNTEPESS SVQPTVSSES STTKANDQST
860 870 880 890 900
AETIELSTAT VPAEASPVED EVRQHSKEEN GTTEASAPST EEAEPAASRD
910 920 930 940 950
AEKQQDETAA TTITVIKPTL ETMETVKEEA KMREEEQTSQ EKSPQENTLP
960
RENVVRQVEE DENYDD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 547 | Q → K in AAA34545 (PubMed:2854095).Curated | 1 | |
Sequence conflicti | 547 | Q → K in AAA35103 (PubMed:3316983).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23440 Genomic DNA Translation: AAA34545.1 M17826 Genomic DNA Translation: AAA35103.1 X66247 Genomic DNA Translation: CAA46973.1 X78993 Genomic DNA Translation: CAA55615.1 Z35981 Genomic DNA Translation: CAA85069.1 BK006936 Genomic DNA Translation: DAA07232.1 |
PIRi | S25365 |
RefSeqi | NP_009670.3, NM_001178460.3 |
Genome annotation databases
EnsemblFungii | YBR112C_mRNA; YBR112C; YBR112C |
GeneIDi | 852410 |
KEGGi | sce:YBR112C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23440 Genomic DNA Translation: AAA34545.1 M17826 Genomic DNA Translation: AAA35103.1 X66247 Genomic DNA Translation: CAA46973.1 X78993 Genomic DNA Translation: CAA55615.1 Z35981 Genomic DNA Translation: CAA85069.1 BK006936 Genomic DNA Translation: DAA07232.1 |
PIRi | S25365 |
RefSeqi | NP_009670.3, NM_001178460.3 |
3D structure databases
AlphaFoldDBi | P14922 |
SMRi | P14922 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32816, 176 interactors |
ComplexPortali | CPX-1663, CYP8-TUP1 corepressor complex |
DIPi | DIP-696N |
IntActi | P14922, 10 interactors |
MINTi | P14922 |
STRINGi | 4932.YBR112C |
PTM databases
iPTMneti | P14922 |
Proteomic databases
MaxQBi | P14922 |
PaxDbi | P14922 |
PRIDEi | P14922 |
Genome annotation databases
EnsemblFungii | YBR112C_mRNA; YBR112C; YBR112C |
GeneIDi | 852410 |
KEGGi | sce:YBR112C |
Organism-specific databases
SGDi | S000000316, CYC8 |
VEuPathDBi | FungiDB:YBR112C |
Phylogenomic databases
eggNOGi | KOG1124, Eukaryota |
GeneTreei | ENSGT00940000168210 |
HOGENOMi | CLU_006762_3_0_1 |
InParanoidi | P14922 |
OMAi | YLGRVEM |
Enzyme and pathway databases
Reactomei | R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-3214842, HDMs demethylate histones |
Miscellaneous databases
PROi | PR:P14922 |
RNActi | P14922, protein |
Family and domain databases
Gene3Di | 1.25.40.10, 3 hits |
InterProi | View protein in InterPro IPR011990, TPR-like_helical_dom_sf IPR001440, TPR_1 IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF00515, TPR_1, 1 hit |
SMARTi | View protein in SMART SM00028, TPR, 10 hits |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50005, TPR, 9 hits PS50293, TPR_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CYC8_YEAST | |
Accessioni | P14922Primary (citable) accession number: P14922 Secondary accession number(s): D6VQB2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | October 5, 2010 | |
Last modified: | May 25, 2022 | |
This is version 223 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - SIMILARITY comments
Index of protein domains and families