UniProtKB - P14920 (OXDA_HUMAN)
Protein
D-amino-acid oxidase
Gene
DAO
Organism
Homo sapiens (Human)
Status
Functioni
Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.1 Publication
Catalytic activityi
- EC:1.4.3.31 Publication
Cofactori
Kineticsi
- KM=3.6 mM for D-serine2 Publications
- KM=1.7 mM for D-proline2 Publications
- KM=1.1 mM for D-tyrosine2 Publications
- KM=1.5 mM for D-DOPA2 Publications
- KM=1.2 mM for D-phenylalanine2 Publications
- KM=0.9 mM for D-alanine2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 53 | Substrate | 1 | |
Binding sitei | 164 | FAD; via amide nitrogen and carbonyl oxygen2 Publications | 1 | |
Binding sitei | 182 | FAD2 Publications | 1 | |
Binding sitei | 217 | Substrate | 1 | |
Binding sitei | 228 | Substrate | 1 | |
Binding sitei | 283 | Substrate | 1 | |
Binding sitei | 313 | Substrate; via carbonyl oxygen | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 3 – 17 | FAD2 PublicationsAdd BLAST | 15 | |
Nucleotide bindingi | 37 – 38 | FAD2 Publications | 2 | |
Nucleotide bindingi | 44 – 45 | FAD2 Publications | 2 | |
Nucleotide bindingi | 49 – 51 | FAD2 Publications | 3 | |
Nucleotide bindingi | 312 – 317 | FAD2 Publications | 6 |
GO - Molecular functioni
- D-amino-acid oxidase activity Source: UniProtKB
- FAD binding Source: UniProtKB
- identical protein binding Source: UniProtKB
GO - Biological processi
- cellular nitrogen compound metabolic process Source: Reactome
- D-alanine catabolic process Source: UniProtKB
- D-amino acid catabolic process Source: GO_Central
- dopamine biosynthetic process Source: UniProtKB
- D-serine catabolic process Source: UniProtKB
- D-serine metabolic process Source: UniProtKB
- proline catabolic process Source: UniProtKB
- protein targeting to peroxisome Source: Reactome
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | MetaCyc:HS03351-MONOMER |
BRENDAi | 1.4.3.3, 2681 |
PathwayCommonsi | P14920 |
Reactomei | R-HSA-389661, Glyoxylate metabolism and glycine degradation R-HSA-9033241, Peroxisomal protein import |
SABIO-RKi | P14920 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DAO Synonyms:DAMOX |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000110887.7 |
HGNCi | HGNC:2671, DAO |
MIMi | 124050, gene |
neXtProti | NX_P14920 |
Subcellular locationi
Peroxisome
Cytosol
- cytosol Source: UniProtKB
Peroxisome
- peroxisomal matrix Source: Reactome
- peroxisomal membrane Source: UniProtKB
- peroxisome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
PeroxisomePathology & Biotechi
Involvement in diseasei
Schizophrenia (SCZD)1 Publication
Disease susceptibility may be associated with variations affecting the gene represented in this entry.
Disease descriptionA complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
Related information in OMIMKeywords - Diseasei
SchizophreniaOrganism-specific databases
DisGeNETi | 1610 |
MalaCardsi | DAO |
MIMi | 181500, phenotype |
OpenTargetsi | ENSG00000110887 |
Orphaneti | 803, Amyotrophic lateral sclerosis |
PharmGKBi | PA27139 |
Miscellaneous databases
Pharosi | P14920, Tchem |
Chemistry databases
ChEMBLi | CHEMBL5485 |
DrugBanki | DB07979, (2E)-3-(3,4-DIHYDROXYPHENYL)-2-IMINOPROPANOIC ACID DB02838, 3,4-Dihydro-2h-Pyrrolium-5-Carboxylate DB04166, Anthranilic acid DB03793, Benzoic acid DB03225, D-Tryptophan DB03147, Flavin adenine dinucleotide DB03531, Flavin-adenine dinucleotide-N5-isobutyl ketone DB02988, Imino-Tryptophan |
DrugCentrali | P14920 |
Polymorphism and mutation databases
BioMutai | DAO |
DMDMi | 25453448 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000162761 | 1 – 347 | D-amino-acid oxidaseAdd BLAST | 347 |
Proteomic databases
MassIVEi | P14920 |
PaxDbi | P14920 |
PeptideAtlasi | P14920 |
PRIDEi | P14920 |
ProteomicsDBi | 53094 |
TopDownProteomicsi | P14920 |
PTM databases
iPTMneti | P14920 |
PhosphoSitePlusi | P14920 |
Expressioni
Gene expression databases
Bgeei | ENSG00000110887, Expressed in liver and 110 other tissues |
ExpressionAtlasi | P14920, baseline and differential |
Genevisiblei | P14920, HS |
Organism-specific databases
HPAi | ENSG00000110887, Group enriched (brain, kidney, liver) |
Interactioni
Subunit structurei
Binary interactionsi
P14920
With | #Exp. | IntAct |
---|---|---|
PRKAB2 [O43741] | 6 | EBI-3908043,EBI-1053424 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107980, 10 interactors |
IntActi | P14920, 5 interactors |
STRINGi | 9606.ENSP00000228476 |
Chemistry databases
BindingDBi | P14920 |
Miscellaneous databases
RNActi | P14920, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14920 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14920 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 345 – 347 | Microbody targeting signal | 3 |
Sequence similaritiesi
Belongs to the DAMOX/DASOX family.Curated
Phylogenomic databases
eggNOGi | KOG3923, Eukaryota |
GeneTreei | ENSGT00390000018635 |
InParanoidi | P14920 |
OMAi | WPYRIEP |
OrthoDBi | 1363414at2759 |
PhylomeDBi | P14920 |
TreeFami | TF313887 |
Family and domain databases
InterProi | View protein in InterPro IPR006181, D-amino_acid_oxidase_CS IPR023209, DAO IPR006076, FAD-dep_OxRdtase |
PANTHERi | PTHR11530, PTHR11530, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
PIRSFi | PIRSF000189, D-aa_oxidase, 1 hit |
PROSITEi | View protein in PROSITE PS00677, DAO, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
P14920-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG
60 70 80 90 100
LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE
110 120 130 140 150
AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE
160 170 180 190 200
RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG
210 220 230 240 250
QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL
260 270 280 290 300
NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP
310 320 330 340
SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4J250 | A0A0B4J250_HUMAN | D-amino-acid oxidase | DAO | 281 | Annotation score: | ||
F8VV35 | F8VV35_HUMAN | D-amino-acid oxidase | DAO | 166 | Annotation score: | ||
F8W152 | F8W152_HUMAN | D-amino-acid oxidase | DAO | 196 | Annotation score: | ||
F8VVT2 | F8VVT2_HUMAN | D-amino-acid oxidase | DAO | 123 | Annotation score: | ||
A0A0B4J257 | A0A0B4J257_HUMAN | D-amino-acid oxidase | DAO | 78 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | D → H in CAA31614 (PubMed:2901986).Curated | 1 | |
Sequence conflicti | 279 | R → A in CAA31614 (PubMed:2901986).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13227 mRNA Translation: CAA31614.1 AK312995 mRNA Translation: BAG35832.1 CH471054 Genomic DNA Translation: EAW97837.1 BC029057 mRNA Translation: AAH29057.1 BC074770 mRNA Translation: AAH74770.1 D11370 Genomic DNA Translation: BAA20974.1 |
CCDSi | CCDS9122.1 |
PIRi | S01340 |
RefSeqi | NP_001908.3, NM_001917.4 XP_011536306.1, XM_011538004.2 XP_011536307.1, XM_011538005.2 |
Genome annotation databases
Ensembli | ENST00000228476; ENSP00000228476; ENSG00000110887 |
GeneIDi | 1610 |
KEGGi | hsa:1610 |
UCSCi | uc001tnr.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13227 mRNA Translation: CAA31614.1 AK312995 mRNA Translation: BAG35832.1 CH471054 Genomic DNA Translation: EAW97837.1 BC029057 mRNA Translation: AAH29057.1 BC074770 mRNA Translation: AAH74770.1 D11370 Genomic DNA Translation: BAA20974.1 |
CCDSi | CCDS9122.1 |
PIRi | S01340 |
RefSeqi | NP_001908.3, NM_001917.4 XP_011536306.1, XM_011538004.2 XP_011536307.1, XM_011538005.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DU8 | X-ray | 2.50 | A/B/G/J | 1-347 | [»] | |
2E48 | X-ray | 2.90 | A/B/C/D | 1-347 | [»] | |
2E49 | X-ray | 3.20 | A/B/C/D | 1-347 | [»] | |
2E4A | X-ray | 2.60 | A/B/C/D | 1-347 | [»] | |
2E82 | X-ray | 2.70 | A/B/C/D | 1-347 | [»] | |
2GNZ | model | - | A | 1-339 | [»] | |
3CUK | X-ray | 2.49 | A/B/C/D | 1-347 | [»] | |
3G3E | X-ray | 2.20 | A/B/C/D | 1-347 | [»] | |
3W4I | X-ray | 2.50 | A/B/C/D | 1-347 | [»] | |
3W4J | X-ray | 2.74 | A/B/C/D | 1-347 | [»] | |
3W4K | X-ray | 2.86 | A/B/C/D | 1-347 | [»] | |
3ZNN | X-ray | 1.90 | A/B | 1-347 | [»] | |
3ZNO | X-ray | 2.30 | A/B | 1-347 | [»] | |
3ZNP | X-ray | 2.40 | A/B | 1-347 | [»] | |
3ZNQ | X-ray | 2.75 | A/B | 1-347 | [»] | |
4QFC | X-ray | 2.40 | A/B | 1-347 | [»] | |
4QFD | X-ray | 2.85 | A/B | 1-347 | [»] | |
5ZJ9 | X-ray | 2.60 | A/B/C/D | 1-340 | [»] | |
5ZJA | X-ray | 2.60 | A/B/C/D | 1-340 | [»] | |
6KBP | X-ray | 2.25 | A/B/C/D | 1-338 | [»] | |
SMRi | P14920 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107980, 10 interactors |
IntActi | P14920, 5 interactors |
STRINGi | 9606.ENSP00000228476 |
Chemistry databases
BindingDBi | P14920 |
ChEMBLi | CHEMBL5485 |
DrugBanki | DB07979, (2E)-3-(3,4-DIHYDROXYPHENYL)-2-IMINOPROPANOIC ACID DB02838, 3,4-Dihydro-2h-Pyrrolium-5-Carboxylate DB04166, Anthranilic acid DB03793, Benzoic acid DB03225, D-Tryptophan DB03147, Flavin adenine dinucleotide DB03531, Flavin-adenine dinucleotide-N5-isobutyl ketone DB02988, Imino-Tryptophan |
DrugCentrali | P14920 |
PTM databases
iPTMneti | P14920 |
PhosphoSitePlusi | P14920 |
Polymorphism and mutation databases
BioMutai | DAO |
DMDMi | 25453448 |
Proteomic databases
MassIVEi | P14920 |
PaxDbi | P14920 |
PeptideAtlasi | P14920 |
PRIDEi | P14920 |
ProteomicsDBi | 53094 |
TopDownProteomicsi | P14920 |
Protocols and materials databases
Antibodypediai | 30788, 283 antibodies |
Genome annotation databases
Ensembli | ENST00000228476; ENSP00000228476; ENSG00000110887 |
GeneIDi | 1610 |
KEGGi | hsa:1610 |
UCSCi | uc001tnr.5, human |
Organism-specific databases
CTDi | 1610 |
DisGeNETi | 1610 |
EuPathDBi | HostDB:ENSG00000110887.7 |
GeneCardsi | DAO |
HGNCi | HGNC:2671, DAO |
HPAi | ENSG00000110887, Group enriched (brain, kidney, liver) |
MalaCardsi | DAO |
MIMi | 124050, gene 181500, phenotype |
neXtProti | NX_P14920 |
OpenTargetsi | ENSG00000110887 |
Orphaneti | 803, Amyotrophic lateral sclerosis |
PharmGKBi | PA27139 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3923, Eukaryota |
GeneTreei | ENSGT00390000018635 |
InParanoidi | P14920 |
OMAi | WPYRIEP |
OrthoDBi | 1363414at2759 |
PhylomeDBi | P14920 |
TreeFami | TF313887 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS03351-MONOMER |
BRENDAi | 1.4.3.3, 2681 |
PathwayCommonsi | P14920 |
Reactomei | R-HSA-389661, Glyoxylate metabolism and glycine degradation R-HSA-9033241, Peroxisomal protein import |
SABIO-RKi | P14920 |
Miscellaneous databases
BioGRID-ORCSi | 1610, 5 hits in 843 CRISPR screens |
ChiTaRSi | DAO, human |
EvolutionaryTracei | P14920 |
GenomeRNAii | 1610 |
Pharosi | P14920, Tchem |
PROi | PR:P14920 |
RNActi | P14920, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000110887, Expressed in liver and 110 other tissues |
ExpressionAtlasi | P14920, baseline and differential |
Genevisiblei | P14920, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006181, D-amino_acid_oxidase_CS IPR023209, DAO IPR006076, FAD-dep_OxRdtase |
PANTHERi | PTHR11530, PTHR11530, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
PIRSFi | PIRSF000189, D-aa_oxidase, 1 hit |
PROSITEi | View protein in PROSITE PS00677, DAO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OXDA_HUMAN | |
Accessioni | P14920Primary (citable) accession number: P14920 Secondary accession number(s): B2R7I5, Q16758, Q8N6R2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | November 25, 2002 | |
Last modified: | December 2, 2020 | |
This is version 195 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM