UniProtKB - P14916 (URE23_HELPY)
Protein
Urease subunit alpha
Gene
ureA
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.1 Publication
Miscellaneous
The novel dodecameric structure of the enzyme may allow it to remain active at the cell surface at acidic gastric pH. Within this dodecameric structure the 12 active sites are clustered within the interior of the proteinaceous shell. This may allow a high local concentration of ammonia within the enzyme which may protect the nickel-chelating groups from protonation.
Caution
The orthologous protein is known as the gamma/beta subunit (UreAB) in most other bacteria.Curated
Catalytic activityi
- EC:3.5.1.5UniRule annotation2 Publications
Kineticsi
- KM=0.48 mM for urea1 Publication
- Vmax=1.1 mmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 8.0. Active from pH 4.0 to 10.0. In unbuffered solutions, the dodecameric complex is active at pH 3.0.2 Publications
: urea degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Urea amidohydrolase (ureB), Urease subunit beta (ureB), Urease (ureA), Urease subunit alpha (ureA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.
GO - Molecular functioni
- nickel cation binding Source: InterPro
- urease activity Source: UniProtKB-UniRule
GO - Biological processi
- pathogenesis Source: UniProtKB-KW
- urea catabolic process Source: CACAO
Keywordsi
Molecular function | Hydrolase |
Biological process | Virulence |
Enzyme and pathway databases
BioCyci | HPY:HP0073-MONOMER |
SABIO-RKi | P14916 |
UniPathwayi | UPA00258;UER00370 |
Names & Taxonomyi
Protein namesi | Recommended name: Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)Alternative name(s): Urea amidohydrolase subunit alphaUniRule annotation |
Gene namesi | Name:ureAUniRule annotation Synonyms:hpuA Ordered Locus Names:HP_0073 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation1 Publication Note: Also associates with the outer membrane upon autolysis of neighboring bacteria.
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells do not express urease.1 Publication
Miscellaneous databases
PHI-basei | PHI:7264 |
Chemistry databases
ChEMBLi | CHEMBL3885651 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098075 | 1 – 238 | Urease subunit alphaAdd BLAST | 238 |
Proteomic databases
PaxDbi | P14916 |
Expressioni
Inductioni
By nickel ions.1 Publication
Interactioni
Subunit structurei
Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits. Four heterohexamers assemble to form a 16 nm dodecameric complex.
UniRule annotation1 PublicationBinary interactionsi
P14916
With | #Exp. | IntAct |
---|---|---|
ureB [P69996] | 2 | EBI-7737170,EBI-7566591 |
Protein-protein interaction databases
DIPi | DIP-3146N |
IntActi | P14916, 7 interactors |
MINTi | P14916 |
STRINGi | 85962.C694_00355 |
Chemistry databases
BindingDBi | P14916 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14916 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14916 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 102 | Urease gammaAdd BLAST | 102 | |
Regioni | 103 – 238 | Urease betaAdd BLAST | 136 |
Sequence similaritiesi
In the N-terminal section; belongs to the urease gamma subunit family.UniRule annotation
In the C-terminal section; belongs to the urease beta subunit family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0831, Bacteria COG0832, Bacteria |
OMAi | DNTGDRP |
Family and domain databases
CDDi | cd00407, Urease_beta, 1 hit cd00390, Urease_gamma, 1 hit |
Gene3Di | 2.10.150.10, 1 hit 3.30.280.10, 1 hit |
HAMAPi | MF_01954, Urease_beta, 1 hit MF_01955, Urease_beta_gamma, 1 hit |
InterProi | View protein in InterPro IPR002019, Urease_beta IPR036461, Urease_betasu_sf IPR008223, Urease_gamma-beta_su IPR002026, Urease_gamma/gamma-beta_su IPR036463, Urease_gamma_sf |
Pfami | View protein in Pfam PF00699, Urease_beta, 1 hit PF00547, Urease_gamma, 1 hit |
PIRSFi | PIRSF001225, Urease_gammabeta, 1 hit |
SUPFAMi | SSF51278, SSF51278, 1 hit SSF54111, SSF54111, 1 hit |
TIGRFAMsi | TIGR00192, urease_beta, 1 hit TIGR00193, urease_gam, 1 hit |
i Sequence
Sequence statusi: Complete.
P14916-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLTPKELDK LMLHYAGELA KKRKEKGIKL NYVEAVALIS AHIMEEARAG
60 70 80 90 100
KKTAAELMQE GRTLLKPDDV MDGVASMIHE VGIEAMFPDG TKLVTVHTPI
110 120 130 140 150
EANGKLVPGE LFLKNEDITI NEGKKAVSVK VKNVGDRPVQ IGSHFHFFEV
160 170 180 190 200
NRCLDFDREK TFGKRLDIAS GTAVRFEPGE EKSVELIDIG GNRRIFGFNA
210 220 230
LVDRQADNES KKIALHRAKE RGFHGAKSDD NYVKTIKE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 14 | H → S AA sequence (PubMed:2318539).Curated | 1 | |
Sequence conflicti | 37 | A → R in CAA34932 (PubMed:2326167).Curated | 1 | |
Sequence conflicti | 49 | A → R in CAA34932 (PubMed:2326167).Curated | 1 | |
Sequence conflicti | 132 – 133 | KN → PP in CAA34932 (PubMed:2326167).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17079 Genomic DNA Translation: CAA34932.1 M60398 Genomic DNA Translation: AAA25020.1 AB032429 Genomic DNA Translation: BAA84532.1 AE000511 Genomic DNA Translation: AAD07144.1 |
PIRi | A38537, URKCAP |
RefSeqi | NP_206873.1, NC_000915.1 WP_000779223.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07144; AAD07144; HP_0073 |
GeneIDi | 48200296 |
KEGGi | heo:C694_00355 hpy:HP0073 |
PATRICi | fig|85962.47.peg.77 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Going unnoticed - Issue 95 of June 2008 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17079 Genomic DNA Translation: CAA34932.1 M60398 Genomic DNA Translation: AAA25020.1 AB032429 Genomic DNA Translation: BAA84532.1 AE000511 Genomic DNA Translation: AAD07144.1 |
PIRi | A38537, URKCAP |
RefSeqi | NP_206873.1, NC_000915.1 WP_000779223.1, NC_018939.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1E9Y | X-ray | 3.00 | A | 1-238 | [»] | |
1E9Z | X-ray | 3.00 | A | 1-238 | [»] | |
SMRi | P14916 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-3146N |
IntActi | P14916, 7 interactors |
MINTi | P14916 |
STRINGi | 85962.C694_00355 |
Chemistry databases
BindingDBi | P14916 |
ChEMBLi | CHEMBL3885651 |
Proteomic databases
PaxDbi | P14916 |
Protocols and materials databases
DNASUi | 900171 |
Genome annotation databases
EnsemblBacteriai | AAD07144; AAD07144; HP_0073 |
GeneIDi | 48200296 |
KEGGi | heo:C694_00355 hpy:HP0073 |
PATRICi | fig|85962.47.peg.77 |
Phylogenomic databases
eggNOGi | COG0831, Bacteria COG0832, Bacteria |
OMAi | DNTGDRP |
Enzyme and pathway databases
UniPathwayi | UPA00258;UER00370 |
BioCyci | HPY:HP0073-MONOMER |
SABIO-RKi | P14916 |
Miscellaneous databases
EvolutionaryTracei | P14916 |
PHI-basei | PHI:7264 |
Family and domain databases
CDDi | cd00407, Urease_beta, 1 hit cd00390, Urease_gamma, 1 hit |
Gene3Di | 2.10.150.10, 1 hit 3.30.280.10, 1 hit |
HAMAPi | MF_01954, Urease_beta, 1 hit MF_01955, Urease_beta_gamma, 1 hit |
InterProi | View protein in InterPro IPR002019, Urease_beta IPR036461, Urease_betasu_sf IPR008223, Urease_gamma-beta_su IPR002026, Urease_gamma/gamma-beta_su IPR036463, Urease_gamma_sf |
Pfami | View protein in Pfam PF00699, Urease_beta, 1 hit PF00547, Urease_gamma, 1 hit |
PIRSFi | PIRSF001225, Urease_gammabeta, 1 hit |
SUPFAMi | SSF51278, SSF51278, 1 hit SSF54111, SSF54111, 1 hit |
TIGRFAMsi | TIGR00192, urease_beta, 1 hit TIGR00193, urease_gam, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | URE23_HELPY | |
Accessioni | P14916Primary (citable) accession number: P14916 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | May 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries