UniProtKB - P14907 (NSP1_YEAST)
Nucleoporin NSP1
NSP1
Functioni
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NSP1 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport.
13 PublicationsMiscellaneous
GO - Molecular functioni
- phospholipid binding Source: SGD
- structural constituent of nuclear pore Source: SGD
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- ncRNA export from nucleus Source: SGD
- NLS-bearing protein import into nucleus Source: SGD
- poly(A)+ mRNA export from nucleus Source: SGD
- protein import into nucleus Source: SGD
- ribosomal large subunit export from nucleus Source: SGD
- ribosomal small subunit export from nucleus Source: SGD
- RNA export from nucleus Source: GO_Central
- tRNA export from nucleus Source: SGD
Keywordsi
Biological process | Cell cycle, mRNA transport, Protein transport, Translocation, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-3232142, SUMOylation of ubiquitinylation proteins R-SCE-4085377, SUMOylation of SUMOylation proteins R-SCE-4551638, SUMOylation of chromatin organization proteins R-SCE-4570464, SUMOylation of RNA binding proteins |
Protein family/group databases
TCDBi | 1.I.1.1.1, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleoporin NSP1Alternative name(s): Nuclear pore protein NSP1 Nucleoskeletal-like protein p110 |
Gene namesi | Name:NSP1 Ordered Locus Names:YJL041W ORF Names:J1207 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003577, NSP1 |
VEuPathDBi | FungiDB:YJL041W |
Subcellular locationi
Nucleus
Note: Symmetric distribution.
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
- nuclear periphery Source: SGD
- nuclear pore Source: SGD
- nuclear pore central transport channel Source: SGD
- nuclear pore nuclear basket Source: SGD
Keywords - Cellular componenti
Membrane, Nuclear pore complex, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204865 | 1 – 823 | Nucleoporin NSP1Add BLAST | 823 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 238 | PhosphoserineCombined sources | 1 | |
Modified residuei | 361 | PhosphoserineCombined sources | 1 | |
Modified residuei | 456 | PhosphoserineCombined sources | 1 | |
Modified residuei | 631 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P14907 |
PaxDbi | P14907 |
PRIDEi | P14907 |
PTM databases
CarbonylDBi | P14907 |
iPTMneti | P14907 |
Interactioni
Subunit structurei
Component of the nuclear pore complex (NPC). NPC constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NSP1 interacts alternatively with NUP82 or NUP57 through its C-terminal coiled coil in two distinct NPC subcomplexes, the NUP82 subcomplex and the NUP57 subcomplex (NIC96, NSP1, NUP49, NUP57). The NUP82 subcomplex is the base for interactions with NUP116 and GLE2 and with NUP42 and GLE1.
Interacts through its FG repeats with karyopherins, such as KAP95, KAP123, PSE1, LOS1, NTF2, the heterodimeric mRNA transport factor MEX67/MTR2, and GSP1.
9 PublicationsBinary interactionsi
P14907
With | #Exp. | IntAct |
---|---|---|
KAP95 [Q06142] | 4 | EBI-12265,EBI-9145 |
NUP57 [P48837] | 5 | EBI-12265,EBI-12324 |
NUP82 [P40368] | 7 | EBI-12265,EBI-12331 |
KPNB1 [Q14974] from Homo sapiens. | 2 | EBI-12265,EBI-286758 |
Protein-protein interaction databases
BioGRIDi | 33717, 138 interactors |
ComplexPortali | CPX-824, Nuclear pore complex |
DIPi | DIP-1484N |
IntActi | P14907, 38 interactors |
MINTi | P14907 |
STRINGi | 4932.YJL041W |
Miscellaneous databases
RNActi | P14907, protein |
Structurei
3D structure databases
BMRBi | P14907 |
SASBDBi | P14907 |
SMRi | P14907 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14907 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 13 – 16 | FSFG 1 | 4 | |
Repeati | 36 – 37 | FG 1 | 2 | |
Repeati | 43 – 44 | FG 2 | 2 | |
Repeati | 64 – 65 | FG 3 | 2 | |
Repeati | 74 – 75 | FG 4 | 2 | |
Repeati | 84 – 85 | FG 5 | 2 | |
Repeati | 94 – 95 | FG 6 | 2 | |
Repeati | 104 – 105 | FG 7 | 2 | |
Repeati | 123 – 124 | FG 8 | 2 | |
Repeati | 142 – 143 | FG 9 | 2 | |
Repeati | 147 – 148 | FG 10 | 2 | |
Repeati | 168 – 169 | FG 11 | 2 | |
Repeati | 179 – 182 | FSFG 2 | 4 | |
Repeati | 217 – 220 | FSFG 3 | 4 | |
Repeati | 239 – 240 | FG 12 | 2 | |
Repeati | 268 – 269 | FG 13 | 2 | |
Repeati | 284 – 287 | FSFG 4 | 4 | |
Repeati | 303 – 306 | FSFG 5 | 4 | |
Repeati | 322 – 325 | FSFG 6 | 4 | |
Repeati | 341 – 344 | FSFG 7 | 4 | |
Repeati | 360 – 363 | FSFG 8 | 4 | |
Repeati | 379 – 382 | FSFG 9 | 4 | |
Repeati | 398 – 401 | FSFG 10 | 4 | |
Repeati | 417 – 420 | FSFG 11 | 4 | |
Repeati | 436 – 439 | FSFG 12 | 4 | |
Repeati | 455 – 458 | FSFG 13 | 4 | |
Repeati | 474 – 477 | FSFG 14 | 4 | |
Repeati | 493 – 496 | FSFG 15 | 4 | |
Repeati | 512 – 515 | FSFG 16 | 4 | |
Repeati | 531 – 534 | FSFG 17 | 4 | |
Repeati | 550 – 553 | FSFG 18 | 4 | |
Repeati | 571 – 572 | FG 14 | 2 | |
Repeati | 588 – 591 | FSFG 19; approximate | 4 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 64 | DisorderedSequence analysisAdd BLAST | 64 | |
Regioni | 70 – 89 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 129 – 620 | DisorderedSequence analysisAdd BLAST | 492 | |
Regioni | 673 – 738 | Competitive binding site of NUP57 and NUP82Add BLAST | 66 | |
Regioni | 740 – 823 | Required for association with NUP57 subcomplexAdd BLAST | 84 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 630 – 823 | Sequence analysisAdd BLAST | 194 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 129 – 186 | Polar residuesSequence analysisAdd BLAST | 58 | |
Compositional biasi | 196 – 288 | Polar residuesSequence analysisAdd BLAST | 93 | |
Compositional biasi | 592 – 606 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG2196, Eukaryota KOG4719, Eukaryota |
HOGENOMi | CLU_020542_0_0_1 |
InParanoidi | P14907 |
OMAi | EMMSKQV |
Family and domain databases
DisProti | DP01077 |
IDEALi | IID50140 |
InterProi | View protein in InterPro IPR026010, NSP1/NUP62 IPR007758, Nucleoporin_NSP1_C |
PANTHERi | PTHR12084, PTHR12084, 1 hit |
Pfami | View protein in Pfam PF05064, Nsp1_C, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNFNTPQQNK TPFSFGTANN NSNTTNQNSS TGAGAFGTGQ STFGFNNSAP
60 70 80 90 100
NNTNNANSSI TPAFGSNNTG NTAFGNSNPT SNVFGSNNST TNTFGSNSAG
110 120 130 140 150
TSLFGSSSAQ QTKSNGTAGG NTFGSSSLFN NSTNSNTTKP AFGGLNFGGG
160 170 180 190 200
NNTTPSSTGN ANTSNNLFGA TANANKPAFS FGATTNDDKK TEPDKPAFSF
210 220 230 240 250
NSSVGNKTDA QAPTTGFSFG SQLGGNKTVN EAAKPSLSFG SGSAGANPAG
260 270 280 290 300
ASQPEPTTNE PAKPALSFGT ATSDNKTTNT TPSFSFGAKS DENKAGATSK
310 320 330 340 350
PAFSFGAKPE EKKDDNSSKP AFSFGAKSNE DKQDGTAKPA FSFGAKPAEK
360 370 380 390 400
NNNETSKPAF SFGAKSDEKK DGDASKPAFS FGAKPDENKA SATSKPAFSF
410 420 430 440 450
GAKPEEKKDD NSSKPAFSFG AKSNEDKQDG TAKPAFSFGA KPAEKNNNET
460 470 480 490 500
SKPAFSFGAK SDEKKDGDAS KPAFSFGAKS DEKKDSDSSK PAFSFGTKSN
510 520 530 540 550
EKKDSGSSKP AFSFGAKPDE KKNDEVSKPA FSFGAKANEK KESDESKSAF
560 570 580 590 600
SFGSKPTGKE EGDGAKAAIS FGAKPEEQKS SDTSKPAFTF GAQKDNEKKT
610 620 630 640 650
EESSTGKSTA DVKSSDSLKL NSKPVELKPV SLDNKTLDDL VTKWTNQLTE
660 670 680 690 700
SASHFEQYTK KINSWDQVLV KGGEQISQLY SDAVMAEHSQ NKIDQSLQYI
710 720 730 740 750
ERQQDELENF LDNFETKTEA LLSDVVSTSS GAAANNNDQK RQQAYKTAQT
760 770 780 790 800
LDENLNSLSS NLSSLIVEIN NVSNTFNKTT NIDINNEDEN IQLIKILNSH
810 820
FDALRSLDDN STSLEKQINS IKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37160 Genomic DNA Translation: AAA34821.1 Z49316 Genomic DNA Translation: CAA89332.1 Z49314 Genomic DNA Translation: CAA89331.1 BK006943 Genomic DNA Translation: DAA08759.1 |
PIRi | S14055 |
RefSeqi | NP_012494.3, NM_001181474.3 |
Genome annotation databases
GeneIDi | 853409 |
KEGGi | sce:YJL041W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37160 Genomic DNA Translation: AAA34821.1 Z49316 Genomic DNA Translation: CAA89332.1 Z49314 Genomic DNA Translation: CAA89331.1 BK006943 Genomic DNA Translation: DAA08759.1 |
PIRi | S14055 |
RefSeqi | NP_012494.3, NM_001181474.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1O6O | X-ray | 2.80 | D/E/F | 497-608 | [»] | |
BMRBi | P14907 | |||||
SASBDBi | P14907 | |||||
SMRi | P14907 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33717, 138 interactors |
ComplexPortali | CPX-824, Nuclear pore complex |
DIPi | DIP-1484N |
IntActi | P14907, 38 interactors |
MINTi | P14907 |
STRINGi | 4932.YJL041W |
Protein family/group databases
TCDBi | 1.I.1.1.1, the nuclear pore complex (npc) family |
PTM databases
CarbonylDBi | P14907 |
iPTMneti | P14907 |
Proteomic databases
MaxQBi | P14907 |
PaxDbi | P14907 |
PRIDEi | P14907 |
Genome annotation databases
GeneIDi | 853409 |
KEGGi | sce:YJL041W |
Organism-specific databases
SGDi | S000003577, NSP1 |
VEuPathDBi | FungiDB:YJL041W |
Phylogenomic databases
eggNOGi | KOG2196, Eukaryota KOG4719, Eukaryota |
HOGENOMi | CLU_020542_0_0_1 |
InParanoidi | P14907 |
OMAi | EMMSKQV |
Enzyme and pathway databases
Reactomei | R-SCE-3232142, SUMOylation of ubiquitinylation proteins R-SCE-4085377, SUMOylation of SUMOylation proteins R-SCE-4551638, SUMOylation of chromatin organization proteins R-SCE-4570464, SUMOylation of RNA binding proteins |
Miscellaneous databases
EvolutionaryTracei | P14907 |
PROi | PR:P14907 |
RNActi | P14907, protein |
Family and domain databases
DisProti | DP01077 |
IDEALi | IID50140 |
InterProi | View protein in InterPro IPR026010, NSP1/NUP62 IPR007758, Nucleoporin_NSP1_C |
PANTHERi | PTHR12084, PTHR12084, 1 hit |
Pfami | View protein in Pfam PF05064, Nsp1_C, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NSP1_YEAST | |
Accessioni | P14907Primary (citable) accession number: P14907 Secondary accession number(s): D6VWE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | February 23, 2022 | |
This is version 206 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families