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Protein

Protein translocation protein SEC63

Gene

SEC63

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the Sec62/63 complex which is involved in SRP-independent post-translational translocation across the endoplasmic reticulum (ER) and functions together with the Sec61 complex and KAR2 in a channel-forming translocon complex. A cycle of assembly and disassembly of Sec62/63 complex from SEC61 may govern the activity of the translocon. SEC63 may affect SEC1-polypeptide interactions by increasing the affinity of targeting pathways for SEC61 and/or by modifying SEC61 to allow more efficient polypeptide interaction. May also be involved in SRP-dependent cotranslational translocation. Is essential for cell growth and for germination.1 Publication

Miscellaneous

Present with 17700 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein transporter activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33745-MONOMER
ReactomeiR-SCE-381038 XBP1(S) activates chaperone genes

Protein family/group databases

TCDBi3.A.5.8.1 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocation protein SEC63
Alternative name(s):
Protein NPL1
Sec62/63 complex 73 kDa subunit
Gene namesi
Name:SEC63
Synonyms:NPL1, PTL1
Ordered Locus Names:YOR254C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

SGDiS000005780 SEC63

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13LumenalSequence analysisAdd BLAST13
Transmembranei14 – 41HelicalSequence analysisAdd BLAST28
Topological domaini42 – 92CytoplasmicSequence analysisAdd BLAST51
Transmembranei93 – 108HelicalSequence analysisAdd BLAST16
Topological domaini109 – 220LumenalSequence analysisAdd BLAST112
Transmembranei221 – 239HelicalSequence analysisAdd BLAST19
Topological domaini240 – 663CytoplasmicSequence analysisAdd BLAST424

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi179A → T: Temperature-sensitive. 1 Publication1
Mutagenesisi426P → L: Temperature-sensitive. 1 Publication1
Mutagenesisi431I → N: Temperature-sensitive. 1 Publication1
Mutagenesisi503P → A: Temperature-sensitive. 1 Publication1
Mutagenesisi511G → R: Temperature-sensitive. 1 Publication1
Mutagenesisi652T → A: Abolishes interaction with SEC62; defect in protein translocation. 1 Publication1
Mutagenesisi654T → A: Abolishes interaction with SEC62; defect in protein translocation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710951 – 663Protein translocation protein SEC63Add BLAST663

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei512PhosphoserineCombined sources1

Post-translational modificationi

Phosphotylated by casein kinase II.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP14906
PaxDbiP14906
PRIDEiP14906

PTM databases

iPTMnetiP14906

Interactioni

Subunit structurei

Component of the heterotetrameric Sec62/63complex composed of SEC62, SEC63, SEC66 and SEC72. The Sec62/63 complex associates with the Sec61 complex to form the Sec complex. SEC63 interacts in its phosphorylated form with SEC62. May physically associate with KAR2 in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis. Part of a complex consisting of KAR2, SEC63, SEC66 and SEC72.5 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi34644, 648 interactors
ComplexPortaliCPX-3055 Translocon complex
CPX-3056 SEC62-SEC63 complex
DIPiDIP-2396N
IntActiP14906, 18 interactors
MINTiP14906
STRINGi4932.YOR254C

Structurei

3D structure databases

ProteinModelPortaliP14906
SMRiP14906
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 198JPROSITE-ProRule annotationAdd BLAST76
Domaini228 – 532SEC63Add BLAST305
Repeati461 – 4711Add BLAST11
Repeati493 – 5032Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni461 – 5032 X 11 AA repeatsAdd BLAST43

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi612 – 663Asp/Glu-rich (highly acidic)Add BLAST52

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000001965
HOGENOMiHOG000248842
InParanoidiP14906
KOiK09540
OMAiANSNDVW
OrthoDBiEOG092C20BT

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR014756 Ig_E-set
IPR036869 J_dom_sf
IPR027137 Sec63
IPR004179 Sec63-dom
PANTHERiPTHR24075:SF0 PTHR24075:SF0, 1 hit
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM00973 Sec63, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit

Sequencei

Sequence statusi: Complete.

P14906-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPTNYEYDEA SETWPSFILT GLLMVVGPMT LLQIYQIFFG ANAEDGNSGK
60 70 80 90 100
SKEFNEEVFK NLNEEYTSDE IKQFRRKFDK NSNKKSKIWS RRNIIIIVGW
110 120 130 140 150
ILVAILLQRI NSNDAIKDAA TKLFDPYEIL GISTSASDRD IKSAYRKLSV
160 170 180 190 200
KFHPDKLAKG LTPDEKSVME ETYVQITKAY ESLTDELVRQ NYLKYGHPDG
210 220 230 240 250
PQSTSHGIAL PRFLVDGSAS PLLVVCYVAL LGLILPYFVS RWWARTQSYT
260 270 280 290 300
KKGIHNVTAS NFVSNLVNYK PSEIVTTDLI LHWLSFAHEF KQFFPDLQPT
310 320 330 340 350
DFEKLLQDHI NRRDSGKLNN AKFRIVAKCH SLLHGLLDIA CGFRNLDIAL
360 370 380 390 400
GAINTFKCIV QAVPLTPNCQ ILQLPNVDKE HFITKTGDIH TLGKLFTLED
410 420 430 440 450
AKIGEVLGIK DQAKLNETLR VASHIPNLKI IKADFLVPGE NQVTPSSTPY
460 470 480 490 500
ISLKVLVRSA KQPLIPTSLI PEENLTEPQD FESQRDPFAM MSKQPLVPYS
510 520 530 540 550
FAPFFPTKRR GSWCCLVSSQ KDGKILQTPI IIEKLSYKNL NDDKDFFDKR
560 570 580 590 600
IKMDLTKHEK FDINDWEIGT IKIPLGQPAP ETVGDFFFRV IVKSTDYFTT
610 620 630 640 650
DLDITMNMKV RDSPAVEQVE VYSEEDDEYS TDDDETESDD ESDASDYTDI
660
DTDTEAEDDE SPE
Length:663
Mass (Da):75,345
Last modified:November 1, 1997 - v2
Checksum:i7D6757144C8A301F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti263V → I in CAA34424 (PubMed:2556404).Curated1
Sequence conflicti293F → L in CAA34424 (PubMed:2556404).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16388 Genomic DNA Translation: CAA34424.1
Z75162 Genomic DNA Translation: CAA99476.1
BK006948 Genomic DNA Translation: DAA11021.1
PIRiA33618
RefSeqiNP_014897.1, NM_001183673.1

Genome annotation databases

EnsemblFungiiYOR254C; YOR254C; YOR254C
GeneIDi854428
KEGGisce:YOR254C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16388 Genomic DNA Translation: CAA34424.1
Z75162 Genomic DNA Translation: CAA99476.1
BK006948 Genomic DNA Translation: DAA11021.1
PIRiA33618
RefSeqiNP_014897.1, NM_001183673.1

3D structure databases

ProteinModelPortaliP14906
SMRiP14906
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34644, 648 interactors
ComplexPortaliCPX-3055 Translocon complex
CPX-3056 SEC62-SEC63 complex
DIPiDIP-2396N
IntActiP14906, 18 interactors
MINTiP14906
STRINGi4932.YOR254C

Protein family/group databases

TCDBi3.A.5.8.1 the general secretory pathway (sec) family

PTM databases

iPTMnetiP14906

Proteomic databases

MaxQBiP14906
PaxDbiP14906
PRIDEiP14906

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR254C; YOR254C; YOR254C
GeneIDi854428
KEGGisce:YOR254C

Organism-specific databases

SGDiS000005780 SEC63

Phylogenomic databases

GeneTreeiENSGT00390000001965
HOGENOMiHOG000248842
InParanoidiP14906
KOiK09540
OMAiANSNDVW
OrthoDBiEOG092C20BT

Enzyme and pathway databases

BioCyciYEAST:G3O-33745-MONOMER
ReactomeiR-SCE-381038 XBP1(S) activates chaperone genes

Miscellaneous databases

PROiPR:P14906

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR014756 Ig_E-set
IPR036869 J_dom_sf
IPR027137 Sec63
IPR004179 Sec63-dom
PANTHERiPTHR24075:SF0 PTHR24075:SF0, 1 hit
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM00973 Sec63, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSEC63_YEAST
AccessioniPrimary (citable) accession number: P14906
Secondary accession number(s): D6W2V5, Q08690
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 185 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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