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Protein

UDP-N-acetylmuramoylalanine--D-glutamate ligase

Gene

murD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi112 – 118ATPSequence analysis7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-UniRule
  • identical protein binding Source: IntAct
  • UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UDP-NACMURALA-GLU-LIG-MONOMER
MetaCyc:UDP-NACMURALA-GLU-LIG-MONOMER
BRENDAi6.3.2.9 2026
SABIO-RKiP14900
UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC:6.3.2.9)
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene namesi
Name:murD
Ordered Locus Names:b0088, JW0086
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10620 murD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4732
DrugBankiDB03801 Lysine Nz-Carboxylic Acid
DB08112 N-({6-[(4-CYANO-2-FLUOROBENZYL)OXY]NAPHTHALEN-2-YL}SULFONYL)-D-GLUTAMIC ACID
DB08108 N-({6-[(4-CYANOBENZYL)OXY]NAPHTHALEN-2-YL}SULFONYL)-D-GLUTAMIC ACID
DB08106 N-[(6-BUTOXYNAPHTHALEN-2-YL)SULFONYL]-D-GLUTAMIC ACID
DB08105 N-[(6-BUTOXYNAPHTHALEN-2-YL)SULFONYL]-L-GLUTAMIC ACID
DB08107 N-{[6-(PENTYLOXY)NAPHTHALEN-2-YL]SULFONYL}-D-GLUTAMIC ACID
DB01673 Uridine-5'-Diphosphate-N-Acetylmuramoyl-L-Alanine
DB02314 Uridine-5'-Diphosphate-N-Acetylmuramoyl-L-Alanine-D-Glutamate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001090132 – 438UDP-N-acetylmuramoylalanine--D-glutamate ligaseAdd BLAST437

Proteomic databases

EPDiP14900
PaxDbiP14900
PRIDEiP14900

Interactioni

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261477, 566 interactors
DIPiDIP-10279N
IntActiP14900, 8 interactors
STRINGi316385.ECDH10B_0070

Chemistry databases

BindingDBiP14900

Structurei

Secondary structure

1438
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP14900
SMRiP14900
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14900

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.Curated

Phylogenomic databases

eggNOGiENOG4105DMZ Bacteria
COG0771 LUCA
HOGENOMiHOG000049427
InParanoidiP14900
KOiK01925
PhylomeDBiP14900

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00639 MurD, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR005762 UDP-N-AcMur-Glu_ligase
PfamiView protein in Pfam
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
TIGRFAMsiTIGR01087 murD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14900-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADYQGKNVV IIGLGLTGLS CVDFFLARGV TPRVMDTRMT PPGLDKLPEA
60 70 80 90 100
VERHTGSLND EWLMAADLIV ASPGIALAHP SLSAAADAGI EIVGDIELFC
110 120 130 140 150
REAQAPIVAI TGSNGKSTVT TLVGEMAKAA GVNVGVGGNI GLPALMLLDD
160 170 180 190 200
ECELYVLELS SFQLETTSSL QAVAATILNV TEDHMDRYPF GLQQYRAAKL
210 220 230 240 250
RIYENAKVCV VNADDALTMP IRGADERCVS FGVNMGDYHL NHQQGETWLR
260 270 280 290 300
VKGEKVLNVK EMKLSGQHNY TNALAALALA DAAGLPRASS LKALTTFTGL
310 320 330 340 350
PHRFEVVLEH NGVRWINDSK ATNVGSTEAA LNGLHVDGTL HLLLGGDGKS
360 370 380 390 400
ADFSPLARYL NGDNVRLYCF GRDGAQLAAL RPEVAEQTET MEQAMRLLAP
410 420 430
RVQPGDMVLL SPACASLDQF KNFEQRGNEF ARLAKELG
Length:438
Mass (Da):46,974
Last modified:January 23, 2007 - v3
Checksum:i11C64782C098F761
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28R → A in CAA35611 (PubMed:2129548).Curated1
Sequence conflicti174A → T in CAA35611 (PubMed:2129548).Curated1
Sequence conflicti276 – 277AL → RV in CAA35611 (PubMed:2129548).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51584 Genomic DNA Translation: CAA35933.1
X17609 Genomic DNA Translation: CAA35611.1
X55034 Genomic DNA Translation: CAA38865.1
M30807 Genomic DNA Translation: AAA83858.1
U00096 Genomic DNA Translation: AAC73199.1
AP009048 Genomic DNA Translation: BAB96656.1
PIRiS08396 CEECME
RefSeqiNP_414630.1, NC_000913.3
WP_000796481.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73199; AAC73199; b0088
BAB96656; BAB96656; BAB96656
GeneIDi944818
KEGGiecj:JW0086
eco:b0088
PATRICifig|1411691.4.peg.2192

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51584 Genomic DNA Translation: CAA35933.1
X17609 Genomic DNA Translation: CAA35611.1
X55034 Genomic DNA Translation: CAA38865.1
M30807 Genomic DNA Translation: AAA83858.1
U00096 Genomic DNA Translation: AAC73199.1
AP009048 Genomic DNA Translation: BAB96656.1
PIRiS08396 CEECME
RefSeqiNP_414630.1, NC_000913.3
WP_000796481.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E0DX-ray2.40A2-438[»]
1EEHX-ray1.90A2-438[»]
1UAGX-ray1.95A2-438[»]
2JFFX-ray1.89A2-438[»]
2JFGX-ray1.52A2-438[»]
2JFHX-ray1.97A2-438[»]
2UAGX-ray1.70A2-438[»]
2UUOX-ray2.50A2-438[»]
2UUPX-ray1.88A2-438[»]
2VTDX-ray1.94A1-438[»]
2VTEX-ray2.20A1-438[»]
2WJPX-ray1.60A2-438[»]
2X5OX-ray1.46A2-438[»]
2XPCX-ray1.49A2-438[»]
2Y1OX-ray1.49A1-438[»]
2Y66X-ray1.49A1-438[»]
2Y67X-ray1.85A1-438[»]
2Y68X-ray1.49A1-438[»]
3UAGX-ray1.77A2-438[»]
4UAGX-ray1.66A2-438[»]
5A5EX-ray1.84A2-438[»]
5A5FX-ray1.90A2-438[»]
ProteinModelPortaliP14900
SMRiP14900
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261477, 566 interactors
DIPiDIP-10279N
IntActiP14900, 8 interactors
STRINGi316385.ECDH10B_0070

Chemistry databases

BindingDBiP14900
ChEMBLiCHEMBL4732
DrugBankiDB03801 Lysine Nz-Carboxylic Acid
DB08112 N-({6-[(4-CYANO-2-FLUOROBENZYL)OXY]NAPHTHALEN-2-YL}SULFONYL)-D-GLUTAMIC ACID
DB08108 N-({6-[(4-CYANOBENZYL)OXY]NAPHTHALEN-2-YL}SULFONYL)-D-GLUTAMIC ACID
DB08106 N-[(6-BUTOXYNAPHTHALEN-2-YL)SULFONYL]-D-GLUTAMIC ACID
DB08105 N-[(6-BUTOXYNAPHTHALEN-2-YL)SULFONYL]-L-GLUTAMIC ACID
DB08107 N-{[6-(PENTYLOXY)NAPHTHALEN-2-YL]SULFONYL}-D-GLUTAMIC ACID
DB01673 Uridine-5'-Diphosphate-N-Acetylmuramoyl-L-Alanine
DB02314 Uridine-5'-Diphosphate-N-Acetylmuramoyl-L-Alanine-D-Glutamate

Proteomic databases

EPDiP14900
PaxDbiP14900
PRIDEiP14900

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73199; AAC73199; b0088
BAB96656; BAB96656; BAB96656
GeneIDi944818
KEGGiecj:JW0086
eco:b0088
PATRICifig|1411691.4.peg.2192

Organism-specific databases

EchoBASEiEB0615
EcoGeneiEG10620 murD

Phylogenomic databases

eggNOGiENOG4105DMZ Bacteria
COG0771 LUCA
HOGENOMiHOG000049427
InParanoidiP14900
KOiK01925
PhylomeDBiP14900

Enzyme and pathway databases

UniPathwayi
UPA00219

BioCyciEcoCyc:UDP-NACMURALA-GLU-LIG-MONOMER
MetaCyc:UDP-NACMURALA-GLU-LIG-MONOMER
BRENDAi6.3.2.9 2026
SABIO-RKiP14900

Miscellaneous databases

EvolutionaryTraceiP14900
PROiPR:P14900

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00639 MurD, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR005762 UDP-N-AcMur-Glu_ligase
PfamiView protein in Pfam
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
TIGRFAMsiTIGR01087 murD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMURD_ECOLI
AccessioniPrimary (citable) accession number: P14900
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 189 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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