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Protein

POU domain, class 2, transcription factor 1

Gene

POU2F1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi379 – 438HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • DNA binding Source: CAFA
  • RNA polymerase II core promoter sequence-specific DNA binding Source: CAFA
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: CAFA
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: UniProtKB
  • transcription factor activity, RNA polymerase II core promoter sequence-specific DNA binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-6785807 Interleukin-4 and 13 signaling
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter
R-HSA-9018519 Estrogen-dependent gene expression
SignaLinkiP14859
SIGNORiP14859

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
NF-A1
Octamer-binding protein 1
Short name:
Oct-1
Octamer-binding transcription factor 1
Short name:
OTF-1
Gene namesi
Name:POU2F1
Synonyms:OCT1, OTF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143190.21
HGNCiHGNC:9212 POU2F1
MIMi164175 gene
neXtProtiNX_P14859

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi385S → A: Loss of inhibition of DNA binding. 1 Publication1

Organism-specific databases

DisGeNETi5451
OpenTargetsiENSG00000143190
PharmGKBiPA33536

Chemistry databases

ChEMBLiCHEMBL3509601

Polymorphism and mutation databases

BioMutaiPOU2F1
DMDMi28202257

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007071 – 743POU domain, class 2, transcription factor 1Add BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei270PhosphothreonineCombined sources1
Modified residuei276PhosphothreonineCombined sources1
Modified residuei283PhosphoserineCombined sources1
Modified residuei385Phosphoserine1 Publication1
Modified residuei448PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PRKDC.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP14859
MaxQBiP14859
PaxDbiP14859
PeptideAtlasiP14859
PRIDEiP14859
ProteomicsDBi53085
53086 [P14859-2]
53087 [P14859-3]
53088 [P14859-4]
53089 [P14859-5]

PTM databases

iPTMnetiP14859
PhosphoSitePlusiP14859

Expressioni

Tissue specificityi

Ubiquitous. Isoform 2 is lymphocyte-specific.

Gene expression databases

BgeeiENSG00000143190
CleanExiHS_POU2F1
ExpressionAtlasiP14859 baseline and differential
GenevisibleiP14859 HS

Organism-specific databases

HPAiCAB002608
HPA058559
HPA064323

Interactioni

Subunit structurei

Interacts with NR3C1, AR, PGR and HCFC1. Associates with the herpes simplex virus VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi111447, 65 interactors
CORUMiP14859
DIPiDIP-226N
IntActiP14859, 32 interactors
MINTiP14859
STRINGi9606.ENSP00000356840

Chemistry databases

BindingDBiP14859

Structurei

Secondary structure

1743
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi285 – 301Combined sources17
Helixi306 – 317Combined sources12
Helixi323 – 331Combined sources9
Helixi336 – 353Combined sources18
Helixi388 – 400Combined sources13
Helixi406 – 416Combined sources11
Helixi420 – 434Combined sources15

3D structure databases

DisProtiDP00231
ProteinModelPortaliP14859
SMRiP14859
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14859

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 354POU-specificPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
GeneTreeiENSGT00760000118935
HOGENOMiHOG000220842
HOVERGENiHBG057016
InParanoidiP14859
KOiK09364
OMAiLGTSQVM
OrthoDBiEOG091G0U0S
PhylomeDBiP14859

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR000972 TF_octamer
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00029 OCTAMER
PR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P14859-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNNPSETSKP SMESGDGNTG TQTNGLDFQK QPVPVGGAIS TAQAQAFLGH
60 70 80 90 100
LHQVQLAGTS LQAAAQSLNV QSKSNEESGD SQQPSQPSQQ PSVQAAIPQT
110 120 130 140 150
QLMLAGGQIT GLTLTPAQQQ LLLQQAQAQA QLLAAAVQQH SASQQHSAAG
160 170 180 190 200
ATISASAATP MTQIPLSQPI QIAQDLQQLQ QLQQQNLNLQ QFVLVHPTTN
210 220 230 240 250
LQPAQFIISQ TPQGQQGLLQ AQNLLTQLPQ QSQANLLQSQ PSITLTSQPA
260 270 280 290 300
TPTRTIAATP IQTLPQSQST PKRIDTPSLE EPSDLEELEQ FAKTFKQRRI
310 320 330 340 350
KLGFTQGDVG LAMGKLYGND FSQTTISRFE ALNLSFKNMC KLKPLLEKWL
360 370 380 390 400
NDAENLSSDS SLSSPSALNS PGIEGLSRRR KKRTSIETNI RVALEKSFLE
410 420 430 440 450
NQKPTSEEIT MIADQLNMEK EVIRVWFCNR RQKEKRINPP SSGGTSSSPI
460 470 480 490 500
KAIFPSPTSL VATTPSLVTS SAATTLTVSP VLPLTSAAVT NLSVTGTSDT
510 520 530 540 550
TSNNTATVIS TAPPASSAVT SPSLSPSPSA SASTSEASSA SETSTTQTTS
560 570 580 590 600
TPLSSPLGTS QVMVTASGLQ TAAAAALQGA AQLPANASLA AMAAAAGLNP
610 620 630 640 650
SLMAPSQFAA GGALLSLNPG TLSGALSPAL MSNSTLATIQ ALASGGSLPI
660 670 680 690 700
TSLDATGNLV FANAGGAPNI VTAPLFLNPQ NLSLLTSNPV SLVSAAAASA
710 720 730 740
GNSAPVASLH ATSTSAESIQ NSLFTVASAS GAASTTTTAS KAQ
Length:743
Mass (Da):76,472
Last modified:February 1, 2003 - v2
Checksum:iFAB27FFC79CF2276
GO
Isoform 2 (identifier: P14859-2) [UniParc]FASTAAdd to basket
Also known as: Oct-1L, lymphocyte-specific

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLDCSDYVLDSRM

Show »
Length:755
Mass (Da):77,870
Checksum:i084E222919B616AA
GO
Isoform 3 (identifier: P14859-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MNNPSETSKPSMESGDGNTG → MKTRMKIFVMIHFHLMNS

Note: No experimental confirmation available.
Show »
Length:741
Mass (Da):76,696
Checksum:i28E72DF211628CE3
GO
Isoform 6 (identifier: P14859-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MADGGAASQDESSAAAAAAADSRM

Note: No experimental confirmation available.
Show »
Length:766
Mass (Da):78,535
Checksum:i54C541CD1D6BA426
GO
Isoform 4 (identifier: P14859-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     641-653: ALASGGSLPITSL → GLLHGLENFLTKN
     654-743: Missing.

Note: No experimental confirmation available.
Show »
Length:653
Mass (Da):68,143
Checksum:i1C82E7818D70A368
GO
Isoform 5 (identifier: P14859-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MADGGAASQDESSAAAAAAADSRM
     112-174: Missing.

Note: No experimental confirmation available.
Show »
Length:703
Mass (Da):72,082
Checksum:i1E6037CDCF41F528
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225L → Q in CAA31767 (PubMed:2905684).Curated1
Sequence conflicti546T → I in AAM77920 (PubMed:12663137).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03581688S → F in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0134211 – 20MNNPS…DGNTG → MKTRMKIFVMIHFHLMNS in isoform 3. CuratedAdd BLAST20
Alternative sequenceiVSP_0023201M → MLDCSDYVLDSRM in isoform 2. 1 Publication1
Alternative sequenceiVSP_0431951M → MADGGAASQDESSAAAAAAA DSRM in isoform 5 and isoform 6. 1 Publication1
Alternative sequenceiVSP_043196112 – 174Missing in isoform 5. 1 PublicationAdd BLAST63
Alternative sequenceiVSP_013422641 – 653ALASG…PITSL → GLLHGLENFLTKN in isoform 4. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_013423654 – 743Missing in isoform 4. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13403 mRNA Translation: CAA31767.1
AY113189 mRNA Translation: AAM77920.1
AK303201 mRNA Translation: BAG64291.1
AL136984 Genomic DNA No translation available.
AL359962 Genomic DNA No translation available.
AL451050 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW90786.1
CH471067 Genomic DNA Translation: EAW90788.1
BC001664 mRNA Translation: AAH01664.1
BC003571 mRNA Translation: AAH03571.1
BC007388 mRNA Translation: AAH07388.1
BC052274 mRNA Translation: AAH52274.1
CCDSiCCDS1259.2 [P14859-6]
CCDS55655.1 [P14859-5]
CCDS55656.1 [P14859-2]
PIRiA47001
RefSeqiNP_001185712.1, NM_001198783.1 [P14859-2]
NP_001185715.1, NM_001198786.1 [P14859-5]
NP_002688.3, NM_002697.3 [P14859-6]
XP_011507956.1, XM_011509654.2 [P14859-1]
UniGeneiHs.283402

Genome annotation databases

EnsembliENST00000367862; ENSP00000356836; ENSG00000143190 [P14859-2]
ENST00000367866; ENSP00000356840; ENSG00000143190 [P14859-6]
ENST00000429375; ENSP00000401217; ENSG00000143190 [P14859-5]
ENST00000541643; ENSP00000441285; ENSG00000143190 [P14859-1]
GeneIDi5451
KEGGihsa:5451
UCSCiuc001gec.4 human [P14859-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPO2F1_HUMAN
AccessioniPrimary (citable) accession number: P14859
Secondary accession number(s): B1AL91
, B1AL93, B4E029, J3KP77, Q5TBT7, Q6PK46, Q8NEU9, Q9BPV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 1, 2003
Last modified: July 18, 2018
This is version 216 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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