UniProtKB - P14859 (PO2F1_HUMAN)
POU domain, class 2, transcription factor 1
POU2F1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 379 – 438 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA binding Source: CAFA
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: CAFA
- identical protein binding Source: IntAct
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: CAFA
- RNA polymerase II core promoter sequence-specific DNA binding Source: CAFA
- sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- cell differentiation Source: GO_Central
- cytokine-mediated signaling pathway Source: Reactome
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- snRNA transcription by RNA polymerase II Source: Reactome
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P14859 |
Reactomei | R-HSA-6785807, Interleukin-4 and Interleukin-13 signaling R-HSA-6807505, RNA polymerase II transcribes snRNA genes R-HSA-749476, RNA Polymerase III Abortive And Retractive Initiation R-HSA-76071, RNA Polymerase III Transcription Initiation From Type 3 Promoter R-HSA-9018519, Estrogen-dependent gene expression |
SignaLinki | P14859 |
SIGNORi | P14859 |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain, class 2, transcription factor 1Alternative name(s): NF-A1 Octamer-binding protein 1 Short name: Oct-1 Octamer-binding transcription factor 1 Short name: OTF-1 |
Gene namesi | Name:POU2F1 Synonyms:OCT1, OTF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000143190.21 |
HGNCi | HGNC:9212, POU2F1 |
MIMi | 164175, gene |
neXtProti | NX_P14859 |
Subcellular locationi
Nucleus
Endoplasmic reticulum
- endoplasmic reticulum Source: HPA
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleoplasm Source: HPA
- nucleus Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: HPA
- RNA polymerase II transcription regulator complex Source: CAFA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 385 | S → A: Loss of inhibition of DNA binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5451 |
OpenTargetsi | ENSG00000143190 |
PharmGKBi | PA33536 |
Miscellaneous databases
Pharosi | P14859, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3509601 |
DrugBanki | DB01203, Nadolol |
Polymorphism and mutation databases
BioMutai | POU2F1 |
DMDMi | 28202257 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100707 | 1 – 743 | POU domain, class 2, transcription factor 1Add BLAST | 743 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 270 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 276 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 283 | PhosphoserineCombined sources | 1 | |
Modified residuei | 385 | Phosphoserine1 Publication | 1 | |
Modified residuei | 448 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P14859 |
jPOSTi | P14859 |
MassIVEi | P14859 |
MaxQBi | P14859 |
PaxDbi | P14859 |
PeptideAtlasi | P14859 |
PRIDEi | P14859 |
ProteomicsDBi | 53085 [P14859-1] 53086 [P14859-2] 53087 [P14859-3] 53088 [P14859-4] 53089 [P14859-5] |
PTM databases
GlyGeni | P14859, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | P14859 |
PhosphoSitePlusi | P14859 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000143190, Expressed in buccal mucosa cell and 230 other tissues |
ExpressionAtlasi | P14859, baseline and differential |
Genevisiblei | P14859, HS |
Organism-specific databases
HPAi | ENSG00000143190, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Interacts with POU2AF1; the interaction increases POU2F1 transactivation activity (PubMed:7859290).
Interacts with NR3C1, AR, PGR and HCFC1.
3 Publications(Microbial infection) Associates with the herpes simplex virus VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.
1 PublicationBinary interactionsi
Hide detailsP14859
With | #Exp. | IntAct |
---|---|---|
PLK3 [Q9H4B4] | 3 | EBI-624770,EBI-751877 |
POGZ [Q7Z3K3] | 3 | EBI-624770,EBI-1389308 |
SP1 [P08047] | 7 | EBI-624770,EBI-298336 |
SP4 [Q02446] | 5 | EBI-624770,EBI-10198587 |
POU2F1 - isoform 6 [P14859-6]
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 111447, 89 interactors |
CORUMi | P14859 |
DIPi | DIP-226N |
IntActi | P14859, 29 interactors |
MINTi | P14859 |
STRINGi | 9606.ENSP00000356840 |
Chemistry databases
BindingDBi | P14859 |
Miscellaneous databases
RNActi | P14859, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P14859 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14859 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 280 – 354 | POU-specificPROSITE-ProRule annotationAdd BLAST | 75 |
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000157831 |
HOGENOMi | CLU_013065_4_0_1 |
InParanoidi | P14859 |
OMAi | IKAIFPC |
OrthoDBi | 873012at2759 |
PhylomeDBi | P14859 |
TreeFami | TF316413 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
DisProti | DP00231 |
Gene3Di | 1.10.260.40, 1 hit |
IDEALi | IID00144 |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR000972, TF_octamer |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00029, OCTAMER PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNNPSETSKP SMESGDGNTG TQTNGLDFQK QPVPVGGAIS TAQAQAFLGH
60 70 80 90 100
LHQVQLAGTS LQAAAQSLNV QSKSNEESGD SQQPSQPSQQ PSVQAAIPQT
110 120 130 140 150
QLMLAGGQIT GLTLTPAQQQ LLLQQAQAQA QLLAAAVQQH SASQQHSAAG
160 170 180 190 200
ATISASAATP MTQIPLSQPI QIAQDLQQLQ QLQQQNLNLQ QFVLVHPTTN
210 220 230 240 250
LQPAQFIISQ TPQGQQGLLQ AQNLLTQLPQ QSQANLLQSQ PSITLTSQPA
260 270 280 290 300
TPTRTIAATP IQTLPQSQST PKRIDTPSLE EPSDLEELEQ FAKTFKQRRI
310 320 330 340 350
KLGFTQGDVG LAMGKLYGND FSQTTISRFE ALNLSFKNMC KLKPLLEKWL
360 370 380 390 400
NDAENLSSDS SLSSPSALNS PGIEGLSRRR KKRTSIETNI RVALEKSFLE
410 420 430 440 450
NQKPTSEEIT MIADQLNMEK EVIRVWFCNR RQKEKRINPP SSGGTSSSPI
460 470 480 490 500
KAIFPSPTSL VATTPSLVTS SAATTLTVSP VLPLTSAAVT NLSVTGTSDT
510 520 530 540 550
TSNNTATVIS TAPPASSAVT SPSLSPSPSA SASTSEASSA SETSTTQTTS
560 570 580 590 600
TPLSSPLGTS QVMVTASGLQ TAAAAALQGA AQLPANASLA AMAAAAGLNP
610 620 630 640 650
SLMAPSQFAA GGALLSLNPG TLSGALSPAL MSNSTLATIQ ALASGGSLPI
660 670 680 690 700
TSLDATGNLV FANAGGAPNI VTAPLFLNPQ NLSLLTSNPV SLVSAAAASA
710 720 730 740
GNSAPVASLH ATSTSAESIQ NSLFTVASAS GAASTTTTAS KAQ
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DG88 | A0A0C4DG88_HUMAN | POU domain protein | POU2F1 | 561 | Annotation score: | ||
A0A0A0MT46 | A0A0A0MT46_HUMAN | POU domain protein | POU2F1 | 594 | Annotation score: | ||
Q16075 | Q16075_HUMAN | Oct-1B | POU2F1 oct-1B | 57 | Annotation score: | ||
A0A0A0MSV5 | A0A0A0MSV5_HUMAN | POU domain, class 2, transcription ... | POU2F1 | 60 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 225 | L → Q in CAA31767 (PubMed:2905684).Curated | 1 | |
Sequence conflicti | 546 | T → I in AAM77920 (PubMed:12663137).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035816 | 88 | S → F in a breast cancer sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013421 | 1 – 20 | MNNPS…DGNTG → MKTRMKIFVMIHFHLMNS in isoform 3. CuratedAdd BLAST | 20 | |
Alternative sequenceiVSP_002320 | 1 | M → MLDCSDYVLDSRM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_043195 | 1 | M → MADGGAASQDESSAAAAAAA DSRM in isoform 5 and isoform 6. 1 Publication | 1 | |
Alternative sequenceiVSP_043196 | 112 – 174 | Missing in isoform 5. 1 PublicationAdd BLAST | 63 | |
Alternative sequenceiVSP_013422 | 641 – 653 | ALASG…PITSL → GLLHGLENFLTKN in isoform 4. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_013423 | 654 – 743 | Missing in isoform 4. 1 PublicationAdd BLAST | 90 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13403 mRNA Translation: CAA31767.1 AY113189 mRNA Translation: AAM77920.1 AK303201 mRNA Translation: BAG64291.1 AL136984 Genomic DNA No translation available. AL359962 Genomic DNA No translation available. AL451050 Genomic DNA No translation available. CH471067 Genomic DNA Translation: EAW90786.1 CH471067 Genomic DNA Translation: EAW90788.1 BC001664 mRNA Translation: AAH01664.1 BC003571 mRNA Translation: AAH03571.1 BC007388 mRNA Translation: AAH07388.1 BC052274 mRNA Translation: AAH52274.1 |
CCDSi | CCDS1259.2 [P14859-6] CCDS55655.1 [P14859-5] CCDS55656.1 [P14859-2] |
PIRi | A47001 |
RefSeqi | NP_001185712.1, NM_001198783.1 [P14859-2] NP_001185715.1, NM_001198786.1 [P14859-5] NP_002688.3, NM_002697.3 [P14859-6] XP_011507956.1, XM_011509654.2 [P14859-1] |
Genome annotation databases
Ensembli | ENST00000367862; ENSP00000356836; ENSG00000143190 [P14859-2] ENST00000367866; ENSP00000356840; ENSG00000143190 [P14859-6] ENST00000429375; ENSP00000401217; ENSG00000143190 [P14859-5] ENST00000541643; ENSP00000441285; ENSG00000143190 [P14859-1] |
GeneIDi | 5451 |
KEGGi | hsa:5451 |
UCSCi | uc001gec.4, human [P14859-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13403 mRNA Translation: CAA31767.1 AY113189 mRNA Translation: AAM77920.1 AK303201 mRNA Translation: BAG64291.1 AL136984 Genomic DNA No translation available. AL359962 Genomic DNA No translation available. AL451050 Genomic DNA No translation available. CH471067 Genomic DNA Translation: EAW90786.1 CH471067 Genomic DNA Translation: EAW90788.1 BC001664 mRNA Translation: AAH01664.1 BC003571 mRNA Translation: AAH03571.1 BC007388 mRNA Translation: AAH07388.1 BC052274 mRNA Translation: AAH52274.1 |
CCDSi | CCDS1259.2 [P14859-6] CCDS55655.1 [P14859-5] CCDS55656.1 [P14859-2] |
PIRi | A47001 |
RefSeqi | NP_001185712.1, NM_001198783.1 [P14859-2] NP_001185715.1, NM_001198786.1 [P14859-5] NP_002688.3, NM_002697.3 [P14859-6] XP_011507956.1, XM_011509654.2 [P14859-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CQT | X-ray | 3.20 | A/B | 278-439 | [»] | |
1E3O | X-ray | 1.90 | C | 280-438 | [»] | |
1GT0 | X-ray | 2.60 | C | 280-438 | [»] | |
1HF0 | X-ray | 2.70 | A/B | 280-438 | [»] | |
1O4X | NMR | - | A | 280-442 | [»] | |
1OCT | X-ray | 3.00 | C | 284-439 | [»] | |
1POG | NMR | - | A | 378-437 | [»] | |
1POU | NMR | - | A | 284-354 | [»] | |
SMRi | P14859 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111447, 89 interactors |
CORUMi | P14859 |
DIPi | DIP-226N |
IntActi | P14859, 29 interactors |
MINTi | P14859 |
STRINGi | 9606.ENSP00000356840 |
Chemistry databases
BindingDBi | P14859 |
ChEMBLi | CHEMBL3509601 |
DrugBanki | DB01203, Nadolol |
PTM databases
GlyGeni | P14859, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | P14859 |
PhosphoSitePlusi | P14859 |
Polymorphism and mutation databases
BioMutai | POU2F1 |
DMDMi | 28202257 |
Proteomic databases
EPDi | P14859 |
jPOSTi | P14859 |
MassIVEi | P14859 |
MaxQBi | P14859 |
PaxDbi | P14859 |
PeptideAtlasi | P14859 |
PRIDEi | P14859 |
ProteomicsDBi | 53085 [P14859-1] 53086 [P14859-2] 53087 [P14859-3] 53088 [P14859-4] 53089 [P14859-5] |
Protocols and materials databases
Antibodypediai | 3616, 621 antibodies |
DNASUi | 5451 |
Genome annotation databases
Ensembli | ENST00000367862; ENSP00000356836; ENSG00000143190 [P14859-2] ENST00000367866; ENSP00000356840; ENSG00000143190 [P14859-6] ENST00000429375; ENSP00000401217; ENSG00000143190 [P14859-5] ENST00000541643; ENSP00000441285; ENSG00000143190 [P14859-1] |
GeneIDi | 5451 |
KEGGi | hsa:5451 |
UCSCi | uc001gec.4, human [P14859-1] |
Organism-specific databases
CTDi | 5451 |
DisGeNETi | 5451 |
EuPathDBi | HostDB:ENSG00000143190.21 |
GeneCardsi | POU2F1 |
HGNCi | HGNC:9212, POU2F1 |
HPAi | ENSG00000143190, Tissue enhanced (brain) |
MIMi | 164175, gene |
neXtProti | NX_P14859 |
OpenTargetsi | ENSG00000143190 |
PharmGKBi | PA33536 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000157831 |
HOGENOMi | CLU_013065_4_0_1 |
InParanoidi | P14859 |
OMAi | IKAIFPC |
OrthoDBi | 873012at2759 |
PhylomeDBi | P14859 |
TreeFami | TF316413 |
Enzyme and pathway databases
PathwayCommonsi | P14859 |
Reactomei | R-HSA-6785807, Interleukin-4 and Interleukin-13 signaling R-HSA-6807505, RNA polymerase II transcribes snRNA genes R-HSA-749476, RNA Polymerase III Abortive And Retractive Initiation R-HSA-76071, RNA Polymerase III Transcription Initiation From Type 3 Promoter R-HSA-9018519, Estrogen-dependent gene expression |
SignaLinki | P14859 |
SIGNORi | P14859 |
Miscellaneous databases
BioGRID-ORCSi | 5451, 53 hits in 870 CRISPR screens |
ChiTaRSi | POU2F1, human |
EvolutionaryTracei | P14859 |
GeneWikii | POU2F1 |
GenomeRNAii | 5451 |
Pharosi | P14859, Tbio |
PROi | PR:P14859 |
RNActi | P14859, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000143190, Expressed in buccal mucosa cell and 230 other tissues |
ExpressionAtlasi | P14859, baseline and differential |
Genevisiblei | P14859, HS |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
DisProti | DP00231 |
Gene3Di | 1.10.260.40, 1 hit |
IDEALi | IID00144 |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR000972, TF_octamer |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00029, OCTAMER PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PO2F1_HUMAN | |
Accessioni | P14859Primary (citable) accession number: P14859 Secondary accession number(s): B1AL91 Q9BPV1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | February 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 234 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations