UniProtKB - P14747 (PP2B2_YEAST)
Protein
Serine/threonine-protein phosphatase 2B catalytic subunit A2
Gene
CMP2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
Miscellaneous
Present with 7110 molecules/cell in log phase SD medium.1 Publication
Caution
Was originally thought to originate from a rabbit cDNA library and was known as protein phosphatase 2Bw (PP2Bw).1 Publication
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
- Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 144 | IronBy similarity | 1 | |
Metal bindingi | 146 | IronBy similarity | 1 | |
Metal bindingi | 172 | IronBy similarity | 1 | |
Metal bindingi | 172 | ZincBy similarity | 1 | |
Metal bindingi | 204 | ZincBy similarity | 1 | |
Active sitei | 205 | Proton donorBy similarity | 1 | |
Metal bindingi | 253 | ZincBy similarity | 1 | |
Metal bindingi | 359 | ZincBy similarity | 1 |
GO - Molecular functioni
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein phosphatase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
- calcineurin-mediated signaling Source: GO_Central
- cellular ion homeostasis Source: SGD
- fungal-type cell wall organization Source: GO_Central
Keywordsi
Molecular function | Calmodulin-binding, Hydrolase, Protein phosphatase |
Ligand | Iron, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-2871809, FCERI mediated Ca+2 mobilization R-SCE-4086398, Ca2+ pathway R-SCE-5607763, CLEC7A (Dectin-1) induces NFAT activation |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase 2B catalytic subunit A2 (EC:3.1.3.16)Alternative name(s): Calcineurin A2 Calmodulin-binding protein 2 |
Gene namesi | Name:CMP2 Synonyms:CNA2 Ordered Locus Names:YML057W ORF Names:YM9958.05 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YML057W |
SGDi | S000004521, CMP2 |
Subcellular locationi
Other locations
- calcineurin complex Source: SGD
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058837 | 1 – 604 | Serine/threonine-protein phosphatase 2B catalytic subunit A2Add BLAST | 604 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 31 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 489 | PhosphoserineCombined sources | 1 | |
Modified residuei | 520 | PhosphoserineSequence analysis | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P14747 |
PaxDbi | P14747 |
PRIDEi | P14747 |
TopDownProteomicsi | P14747 |
PTM databases
iPTMneti | P14747 |
Interactioni
Subunit structurei
Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.
Binary interactionsi
P14747
With | #Exp. | IntAct |
---|---|---|
CNB1 [P25296] | 5 | EBI-12778,EBI-3968 |
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 35110, 133 interactors |
ComplexPortali | CPX-590, Calcineurin complex variant 2 |
DIPi | DIP-833N |
IntActi | P14747, 28 interactors |
MINTi | P14747 |
STRINGi | 4932.YML057W |
Miscellaneous databases
RNActi | P14747, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 501 – 523 | Calmodulin-bindingSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0375, Eukaryota |
GeneTreei | ENSGT00940000176583 |
HOGENOMi | CLU_004962_6_2_1 |
InParanoidi | P14747 |
OMAi | ACMNTFD |
Family and domain databases
CDDi | cd07416, MPP_PP2B, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR041751, MPP_PP2B IPR043360, PP2B IPR006186, Ser/Thr-sp_prot-phosphatase |
PANTHERi | PTHR45673, PTHR45673, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P14747-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSDAIRNTE QINAAIKIIE NKTERPQSST TPIDSKASTV AAANSTATET
60 70 80 90 100
SRDLTQYTLD DGRVVSTNRR IMNKVPAITS HVPTDEELFQ PNGIPRHEFL
110 120 130 140 150
RDHFKREGKL SAAQAARIVT LATELFSKEP NLISVPAPIT VCGDIHGQYF
160 170 180 190 200
DLLKLFEVGG DPATTSYLFL GDYVDRGSFS FECLIYLYSL KLNFNDHFWL
210 220 230 240 250
LRGNHECKHL TSYFTFKNEM LHKYNLDIYE KCCESFNNLP LAALMNGQYL
260 270 280 290 300
CVHGGISPEL NSLQDINNLN RFREIPSHGL MCDLLWADPI EEYDEVLDKD
310 320 330 340 350
LTEEDIVNSK TMVPHHGKMA PSRDMFVPNS VRGCSYAFTY RAACHFLQET
360 370 380 390 400
GLLSIIRAHE AQDAGYRMYK NTKTLGFPSL LTLFSAPNYL DTYNNKAAIL
410 420 430 440 450
KYENNVMNIR QFNMTPHPYW LPDFMDVFTW SLPFVGEKVT EMLVAILNIC
460 470 480 490 500
TEDELENDTP VIEELVGTDK KLPQAGKSEA TPQPATSASP KHASILDDEH
510 520 530 540 550
RRKALRNKIL AVAKVSRMYS VLREETNKVQ FLKDHNSGVL PRGALSNGVK
560 570 580 590 600
GLDEALSTFE RARKHDLINE KLPPSLDELK NENKKYYEKV WQKVHEHDAK
NDSK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 87 – 88 | EL → DV in CAA38712 (PubMed:1646387).Curated | 2 | |
Sequence conflicti | 581 | N → K in CAA34722 (PubMed:2557079).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64840 Genomic DNA Translation: AAA34466.1 X16804 mRNA Translation: CAA34722.1 Z46729 Genomic DNA Translation: CAA86718.1 X54964 Genomic DNA Translation: CAA38712.1 BK006946 Genomic DNA Translation: DAA09841.1 |
PIRi | S49804 |
RefSeqi | NP_013655.1, NM_001182415.1 |
Genome annotation databases
EnsemblFungii | YML057W_mRNA; YML057W; YML057W |
GeneIDi | 854946 |
KEGGi | sce:YML057W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64840 Genomic DNA Translation: AAA34466.1 X16804 mRNA Translation: CAA34722.1 Z46729 Genomic DNA Translation: CAA86718.1 X54964 Genomic DNA Translation: CAA38712.1 BK006946 Genomic DNA Translation: DAA09841.1 |
PIRi | S49804 |
RefSeqi | NP_013655.1, NM_001182415.1 |
3D structure databases
SMRi | P14747 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35110, 133 interactors |
ComplexPortali | CPX-590, Calcineurin complex variant 2 |
DIPi | DIP-833N |
IntActi | P14747, 28 interactors |
MINTi | P14747 |
STRINGi | 4932.YML057W |
PTM databases
iPTMneti | P14747 |
Proteomic databases
MaxQBi | P14747 |
PaxDbi | P14747 |
PRIDEi | P14747 |
TopDownProteomicsi | P14747 |
Genome annotation databases
EnsemblFungii | YML057W_mRNA; YML057W; YML057W |
GeneIDi | 854946 |
KEGGi | sce:YML057W |
Organism-specific databases
EuPathDBi | FungiDB:YML057W |
SGDi | S000004521, CMP2 |
Phylogenomic databases
eggNOGi | KOG0375, Eukaryota |
GeneTreei | ENSGT00940000176583 |
HOGENOMi | CLU_004962_6_2_1 |
InParanoidi | P14747 |
OMAi | ACMNTFD |
Enzyme and pathway databases
Reactomei | R-SCE-2871809, FCERI mediated Ca+2 mobilization R-SCE-4086398, Ca2+ pathway R-SCE-5607763, CLEC7A (Dectin-1) induces NFAT activation |
Miscellaneous databases
PROi | PR:P14747 |
RNActi | P14747, protein |
Family and domain databases
CDDi | cd07416, MPP_PP2B, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR041751, MPP_PP2B IPR043360, PP2B IPR006186, Ser/Thr-sp_prot-phosphatase |
PANTHERi | PTHR45673, PTHR45673, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP2B2_YEAST | |
Accessioni | P14747Primary (citable) accession number: P14747 Secondary accession number(s): D6VZB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | November 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 196 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD