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UniProtKB - P14742 (GFA1_YEAST)

Entry version 197 (29 Sep 2021)
Sequence version 4 (23 Jan 2007)
Previous versions | rss
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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

GFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).

1 Publication

Miscellaneous

Present with 14300 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate. This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei2For GATase activity1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminotransferase, Transferase

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.6.1.16, 984

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-SCE-446210, Synthesis of UDP-N-acetyl-glucosamine

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00113;UER00528

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:GFA1
Ordered Locus Names:YKL104C
ORF Names:YKL457
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XI

Organism-specific databases

Saccharomyces Genome Database

More...SGDi		S000001587, GFA1

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		FungiDB:YKL104C

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved1 Publication
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001352892 – 717Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei253PhosphoserineCombined sources1
Modified residuei334PhosphothreonineCombined sources1
Modified residuei336PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...MaxQBi		P14742

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P14742

PRoteomics IDEntifications database

More...PRIDEi		P14742

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P14742

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

The activity of this enzyme increases in presence of mating pheromone (transcriptional regulation).

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		34030, 213 interactors

Database of interacting proteins

More...DIPi		DIP-5224N

Protein interaction database and analysis system

More...IntActi		P14742, 144 interactors

Molecular INTeraction database

More...MINTi		P14742

STRING: functional protein association networks

More...STRINGi		4932.YKL104C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P14742, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P14742

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini2 – 318Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST317
Domaini390 – 529SIS 1PROSITE-ProRule annotationAdd BLAST140
Domaini562 – 707SIS 2PROSITE-ProRule annotationAdd BLAST146

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1268, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000173234

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_012520_5_2_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P14742

Identification of Orthologs from Complete Genome Data

More...OMAi		AYTHAGP

Family and domain databases

Conserved Domains Database

More...CDDi		cd05008, SIS_GlmS_GlmD_1, 1 hit
cd05009, SIS_GlmS_GlmD_2, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR017932, GATase_2_dom
IPR035466, GlmS/AgaS_SIS
IPR035490, GlmS/FrlB_SIS
IPR029055, Ntn_hydrolases_N
IPR001347, SIS_dom

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01380, SIS, 2 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF56235, SSF56235, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51278, GATASE_TYPE_2, 1 hit
PS51464, SIS, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P14742-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCGIFGYCNY LVERSRGEII DTLVDGLQRL EYRGYDSTGI AIDGDEADST 
        60         70         80         90        100
FIYKQIGKVS ALKEEITKQN PNRDVTFVSH CGIAHTRWAT HGRPEQVNCH 
       110        120        130        140        150
PQRSDPEDQF VVVHNGIITN FRELKTLLIN KGYKFESDTD TECIAKLYLH 
       160        170        180        190        200
LYNTNLQNGH DLDFHELTKL VLLELEGSYG LLCKSCHYPN EVIATRKGSP 
       210        220        230        240        250
LLIGVKSEKK LKVDFVDVEF PEENAGQPEI PLKSNNKSFG LGPKKAREFE 
       260        270        280        290        300
AGSQNANLLP IAANEFNLRH SQSRAFLSED GSPTPVEFFV SSDAASVVKH 
       310        320        330        340        350
TKKVLFLEDD DLAHIYDGEL HIHRSRREVG ASMTRSIQTL EMELAQIMKG 
       360        370        380        390        400
PYDHFMQKEI YEQPESTFNT MRGRIDYENN KVILGGLKAW LPVVRRARRL 
       410        420        430        440        450
IMIACGTSYH SCLATRAIFE ELSDIPVSVE LASDFLDRKC PVFRDDVCVF 
       460        470        480        490        500
VSQSGETADT MLALNYCLER GALTVGIVNS VGSSISRVTH CGVHINAGPE 
       510        520        530        540        550
IGVASTKAYT SQYIALVMFA LSLSDDRVSK IDRRIEIIQG LKLIPGQIKQ 
       560        570        580        590        600
VLKLEPRIKK LCATELKDQK SLLLLGRGYQ FAAALEGALK IKEISYMHSE 
       610        620        630        640        650
GVLAGELKHG VLALVDENLP IIAFGTRDSL FPKVVSSIEQ VTARKGHPII 
       660        670        680        690        700
ICNENDEVWA QKSKSIDLQT LEVPQTVDCL QGLINIIPLQ LMSYWLAVNK 
       710 
GIDVDFPRNL AKSVTVE
Length:717
Mass (Da):80,047
Last modified:January 23, 2007 - v4
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i27E5F0D24BDC451A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti453 – 454QS → HC in AAA34643 (PubMed:2656689).Curated2
Sequence conflicti534Missing in AAA34643 (PubMed:2656689).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
J04719 Genomic DNA Translation: AAA34643.1
X71133 Genomic DNA Translation: CAA50453.1
Z28104 Genomic DNA Translation: CAA81944.1
BK006944 Genomic DNA Translation: DAA09054.1

Protein sequence database of the Protein Information Resource

More...PIRi		S37931, XNBYGM

NCBI Reference Sequences

More...RefSeqi		NP_012818.1, NM_001179670.1

Genome annotation databases

Ensembl fungal genome annotation project

More...EnsemblFungii		YKL104C_mRNA; YKL104C; YKL104C

Database of genes from NCBI RefSeq genomes

More...GeneIDi		853757

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		sce:YKL104C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04719 Genomic DNA Translation: AAA34643.1
X71133 Genomic DNA Translation: CAA50453.1
Z28104 Genomic DNA Translation: CAA81944.1
BK006944 Genomic DNA Translation: DAA09054.1
PIRiS37931, XNBYGM
RefSeqiNP_012818.1, NM_001179670.1

3D structure databases

SMRiP14742
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi34030, 213 interactors
DIPiDIP-5224N
IntActiP14742, 144 interactors
MINTiP14742
STRINGi4932.YKL104C

PTM databases

iPTMnetiP14742

Proteomic databases

MaxQBiP14742
PaxDbiP14742
PRIDEiP14742

Genome annotation databases

EnsemblFungiiYKL104C_mRNA; YKL104C; YKL104C
GeneIDi853757
KEGGisce:YKL104C

Organism-specific databases

SGDiS000001587, GFA1
VEuPathDBiFungiDB:YKL104C

Phylogenomic databases

eggNOGiKOG1268, Eukaryota
GeneTreeiENSGT00940000173234
HOGENOMiCLU_012520_5_2_1
InParanoidiP14742
OMAiAYTHAGP

Enzyme and pathway databases

UniPathwayiUPA00113;UER00528
BRENDAi2.6.1.16, 984
ReactomeiR-SCE-446210, Synthesis of UDP-N-acetyl-glucosamine

Miscellaneous databases

Protein Ontology

More...PROi		PR:P14742
RNActiP14742, protein

Family and domain databases

CDDicd05008, SIS_GlmS_GlmD_1, 1 hit
cd05009, SIS_GlmS_GlmD_2, 1 hit
InterProiView protein in InterPro
IPR017932, GATase_2_dom
IPR035466, GlmS/AgaS_SIS
IPR035490, GlmS/FrlB_SIS
IPR029055, Ntn_hydrolases_N
IPR001347, SIS_dom
PfamiView protein in Pfam
PF01380, SIS, 2 hits
SUPFAMiSSF56235, SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS51278, GATASE_TYPE_2, 1 hit
PS51464, SIS, 2 hits

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGFA1_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P14742
Secondary accession number(s): D6VXI4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: September 29, 2021
This is version 197 of the entry and version 4 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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