UniProtKB - P14742 (GFA1_YEAST)
Protein
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Gene
GFA1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).1 Publication
Miscellaneous
Present with 14300 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.6.1.161 Publication
: UDP-N-acetyl-alpha-D-glucosamine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.Proteins known to be involved in this subpathway in this organism are:
- Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing] (YMR084W), Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (GFA1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | For GATase activity1 Publication | 1 |
GO - Molecular functioni
- carbohydrate derivative binding Source: InterPro
- glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: SGD
GO - Biological processi
- fructose 6-phosphate metabolic process Source: GO_Central
- fungal-type cell wall chitin biosynthetic process Source: SGD
- glutamine metabolic process Source: UniProtKB-KW
- protein N-linked glycosylation Source: GO_Central
- UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
- UDP-N-acetylglucosamine metabolic process Source: GO_Central
Keywordsi
Molecular function | Aminotransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-SCE-446210, Synthesis of UDP-N-acetyl-glucosamine |
UniPathwayi | UPA00113;UER00528 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)Short name: GFAT Alternative name(s): D-fructose-6-phosphate amidotransferase Hexosephosphate aminotransferase |
Gene namesi | Name:GFA1 Ordered Locus Names:YKL104C ORF Names:YKL457 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001587, GFA1 |
VEuPathDBi | FungiDB:YKL104C |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000135289 | 2 – 717 | Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST | 716 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 253 | PhosphoserineCombined sources | 1 | |
Modified residuei | 334 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 336 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P14742 |
PaxDbi | P14742 |
PRIDEi | P14742 |
PTM databases
iPTMneti | P14742 |
Expressioni
Inductioni
The activity of this enzyme increases in presence of mating pheromone (transcriptional regulation).
Interactioni
Protein-protein interaction databases
BioGRIDi | 34030, 211 interactors |
DIPi | DIP-5224N |
IntActi | P14742, 144 interactors |
MINTi | P14742 |
STRINGi | 4932.YKL104C |
Miscellaneous databases
RNActi | P14742, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 318 | Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST | 317 | |
Domaini | 390 – 529 | SIS 1PROSITE-ProRule annotationAdd BLAST | 140 | |
Domaini | 562 – 707 | SIS 2PROSITE-ProRule annotationAdd BLAST | 146 |
Keywords - Domaini
Glutamine amidotransferase, RepeatPhylogenomic databases
eggNOGi | KOG1268, Eukaryota |
GeneTreei | ENSGT00940000173234 |
HOGENOMi | CLU_012520_5_2_1 |
InParanoidi | P14742 |
OMAi | AYTHAGP |
Family and domain databases
CDDi | cd05008, SIS_GlmS_GlmD_1, 1 hit cd05009, SIS_GlmS_GlmD_2, 1 hit |
InterProi | View protein in InterPro IPR017932, GATase_2_dom IPR035466, GlmS/AgaS_SIS IPR035490, GlmS/FrlB_SIS IPR029055, Ntn_hydrolases_N IPR001347, SIS |
Pfami | View protein in Pfam PF01380, SIS, 2 hits |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit PS51464, SIS, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P14742-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCGIFGYCNY LVERSRGEII DTLVDGLQRL EYRGYDSTGI AIDGDEADST
60 70 80 90 100
FIYKQIGKVS ALKEEITKQN PNRDVTFVSH CGIAHTRWAT HGRPEQVNCH
110 120 130 140 150
PQRSDPEDQF VVVHNGIITN FRELKTLLIN KGYKFESDTD TECIAKLYLH
160 170 180 190 200
LYNTNLQNGH DLDFHELTKL VLLELEGSYG LLCKSCHYPN EVIATRKGSP
210 220 230 240 250
LLIGVKSEKK LKVDFVDVEF PEENAGQPEI PLKSNNKSFG LGPKKAREFE
260 270 280 290 300
AGSQNANLLP IAANEFNLRH SQSRAFLSED GSPTPVEFFV SSDAASVVKH
310 320 330 340 350
TKKVLFLEDD DLAHIYDGEL HIHRSRREVG ASMTRSIQTL EMELAQIMKG
360 370 380 390 400
PYDHFMQKEI YEQPESTFNT MRGRIDYENN KVILGGLKAW LPVVRRARRL
410 420 430 440 450
IMIACGTSYH SCLATRAIFE ELSDIPVSVE LASDFLDRKC PVFRDDVCVF
460 470 480 490 500
VSQSGETADT MLALNYCLER GALTVGIVNS VGSSISRVTH CGVHINAGPE
510 520 530 540 550
IGVASTKAYT SQYIALVMFA LSLSDDRVSK IDRRIEIIQG LKLIPGQIKQ
560 570 580 590 600
VLKLEPRIKK LCATELKDQK SLLLLGRGYQ FAAALEGALK IKEISYMHSE
610 620 630 640 650
GVLAGELKHG VLALVDENLP IIAFGTRDSL FPKVVSSIEQ VTARKGHPII
660 670 680 690 700
ICNENDEVWA QKSKSIDLQT LEVPQTVDCL QGLINIIPLQ LMSYWLAVNK
710
GIDVDFPRNL AKSVTVE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 453 – 454 | QS → HC in AAA34643 (PubMed:2656689).Curated | 2 | |
Sequence conflicti | 534 | Missing in AAA34643 (PubMed:2656689).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04719 Genomic DNA Translation: AAA34643.1 X71133 Genomic DNA Translation: CAA50453.1 Z28104 Genomic DNA Translation: CAA81944.1 BK006944 Genomic DNA Translation: DAA09054.1 |
PIRi | S37931, XNBYGM |
RefSeqi | NP_012818.1, NM_001179670.1 |
Genome annotation databases
EnsemblFungii | YKL104C_mRNA; YKL104C; YKL104C |
GeneIDi | 853757 |
KEGGi | sce:YKL104C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04719 Genomic DNA Translation: AAA34643.1 X71133 Genomic DNA Translation: CAA50453.1 Z28104 Genomic DNA Translation: CAA81944.1 BK006944 Genomic DNA Translation: DAA09054.1 |
PIRi | S37931, XNBYGM |
RefSeqi | NP_012818.1, NM_001179670.1 |
3D structure databases
SMRi | P14742 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34030, 211 interactors |
DIPi | DIP-5224N |
IntActi | P14742, 144 interactors |
MINTi | P14742 |
STRINGi | 4932.YKL104C |
PTM databases
iPTMneti | P14742 |
Proteomic databases
MaxQBi | P14742 |
PaxDbi | P14742 |
PRIDEi | P14742 |
Genome annotation databases
EnsemblFungii | YKL104C_mRNA; YKL104C; YKL104C |
GeneIDi | 853757 |
KEGGi | sce:YKL104C |
Organism-specific databases
SGDi | S000001587, GFA1 |
VEuPathDBi | FungiDB:YKL104C |
Phylogenomic databases
eggNOGi | KOG1268, Eukaryota |
GeneTreei | ENSGT00940000173234 |
HOGENOMi | CLU_012520_5_2_1 |
InParanoidi | P14742 |
OMAi | AYTHAGP |
Enzyme and pathway databases
UniPathwayi | UPA00113;UER00528 |
Reactomei | R-SCE-446210, Synthesis of UDP-N-acetyl-glucosamine |
Miscellaneous databases
PROi | PR:P14742 |
RNActi | P14742, protein |
Family and domain databases
CDDi | cd05008, SIS_GlmS_GlmD_1, 1 hit cd05009, SIS_GlmS_GlmD_2, 1 hit |
InterProi | View protein in InterPro IPR017932, GATase_2_dom IPR035466, GlmS/AgaS_SIS IPR035490, GlmS/FrlB_SIS IPR029055, Ntn_hydrolases_N IPR001347, SIS |
Pfami | View protein in Pfam PF01380, SIS, 2 hits |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit PS51464, SIS, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GFA1_YEAST | |
Accessioni | P14742Primary (citable) accession number: P14742 Secondary accession number(s): D6VXI4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 195 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways