UniProtKB - P14737 (RAD9_YEAST)

BLAST

>sp|P14737|RAD9_YEAST DNA repair protein RAD9 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RAD9 PE=1 SV=2
MSGQLVQWKSSPDRVTQSAIKEALHSPLADGDMNEMNVPVDPLENKVNSTNIIEGSPKAN
PNPVKFMNTSEIFQKSLGLLDESPRHDDELNIEVGDNDRPNANILHNERTPDLDRIANFF
KSNRTPGKENLLTKYQSSDLEDTPLMLRKKMTFQTPTDPLEQKTFKKLKSDTGFCYYGEQ
NDGEENASLEVTEADATFVQMAERSADNYDCALEGIVTPKRYKDELSKSGGMQDERVQKT
QIMISAESPNSISSYDKNKITGNGRTTRNVNKVFNNNEDNIGAIEEKNPVKKKSENYSSD
DLRERNNQIIQSNESEEINELEKNLNVSGRENDVNNLDIDINSAVSGTPSRNNAEEEMYS
SESVNNREPSKKWIFRYSKDKTENNSNRSTQIVNNPRTQEMPLDSISIDTQPLSKSFNTE
TNNELETQIIVSSLSQGISAQKGPVFHSTGQTEEIKTQIINSPEQNALNATFETPVTLSR
INFEPILEVPETSSPSKNTMSKPSNSSPIPKEKDTFNIHEREVETNNVFSNDIQNSSNAA
TRDDIIIAGSSDFNEQKEITDRIYLQLSGKQISDSGSDETERMSPNELDTKKESTIMSEV
ELTQELPEVEEQQDLQTSPKKLVVEEETLMEIKKSKGNSLQLHDDNKECNSDKQDGTESL
DVALIEHESKGQSSELQKNLMQLFPSESQEIIQNRRTIKRRQKDTIEIGEEEENRSTKTS
PTKHLKRNSDLDAASIKREPSCSITIQTGETGSGKDSKEQSYVFPEGIRTADNSFLSKDD
IIFGNAVWCQYTWNYKFYPGILLEVDTNQDGCWIYFETGRSLTKDEDIYYLDIRIGDAVT
FDGNEYVVVGLECRSHDLNIIRCIRGYDTVHLKKKNASGLLGKRTLIKALSSISLDLSEW
AKRAKIILEDNEKNKGDAYRYLRHPIRGRKSMTNVLSPKKHTDDEKDINTHTEVYNNEIE
SSSEKKEIVKKDSRDALAEHAGAPSLLFSSGEIRTGNVFDKCIFVLTSLFENREELRQTI
ESQGGTVIESGFSTLFNFTHPLAKSLVNKGNTDNIRELALKLAWKPHSLFADCRFACLIT
KRHLRSLKYLETLALGWPTLHWKFISACIEKKRIVPHLIYQYLLPSGESFRLSLDSPSKG
GIIKSNNIFSFYTQFLRGSNLRDQICGVKKMLNDYIVIVWGRSELDSFVKFAFACLSAGR
MLTIDLPNIDVDDTEPLLNALDSLVPRIGSELSNRKLKFLIYANENNGKSQMKLLERLRS
QISLKFKKFNYIFHTESKEWLIQTIINEDTGFHDDITDNDIYNTISEVR

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History
Entry version 175 (07 Nov 2018)
Sequence version 2 (05 Oct 2010)
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Protein

DNA repair protein RAD9

Gene

RAD9

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Essential for cell cycle arrest at the G2 stage following DNA damage by X-irradiation or inactivation of DNA ligase.

Miscellaneous

Present with 400 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functionⁱ

double-stranded DNA binding
Source: SGD
Inferred from direct assayⁱ
- 17726056
enzyme activator activity
Source: SGD
Inferred from mutant phenotypeⁱ
- 24302356
histone binding
Source: SGD
Inferred from direct assayⁱ
- 17721446

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA damage checkpoint
Source: SGD
Inferred from mutant phenotypeⁱ
- 9564050
DNA repair
Source: SGD
Inferred from mutant phenotypeⁱ
- 2664461
double-strand break repair
Source: SGD
Inferred from mutant phenotypeⁱ
- 26490958
G1 DNA damage checkpoint
Source: SGD
Inferred from mutant phenotypeⁱ
- 16166626
intra-S DNA damage checkpoint
Source: SGD
Inferred from mutant phenotypeⁱ
- 16166626
- 17721446
mitotic G1 DNA damage checkpoint
Source: SGD
Inferred from mutant phenotypeⁱ
- 17721446
negative regulation of DNA replication Source: UniProtKB-KW
nucleotide-excision repair
Source: SGD
Inferred from mutant phenotypeⁱ
- 11267834
positive regulation of transcription by RNA polymerase II
Source: SGD
Inferred from mutant phenotypeⁱ
- 8670896
regulation of cell cycle
Source: SGD
Inferred from mutant phenotypeⁱ
- 2664461

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA replication inhibitor
Biological process	Cell cycle, DNA damage

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29798-MONOMER

BioCycⁱ

YEAST:G3O-29798-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA repair protein RAD9
Gene namesⁱ	Name:RAD9 Ordered Locus Names:YDR217C ORF Names:YD9934.02C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IV

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000002625 RAD9

SGDⁱ

S000002625 RAD9

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000097158	1 – 1309	DNA repair protein RAD9Add BLAST		1309

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	26	PhosphoserineCombined sources	1
Modified residueⁱ	56	PhosphoserineCombined sources	1
Modified residueⁱ	205	PhosphoserineCombined sources	1
Modified residueⁱ	218	PhosphothreonineCombined sources	1
Modified residueⁱ	248	PhosphoserineCombined sources	1
Modified residueⁱ	312	PhosphoserineCombined sources	1
Modified residueⁱ	315	PhosphoserineCombined sources	1
Modified residueⁱ	462	PhosphoserineCombined sources	1
Modified residueⁱ	471	PhosphothreonineCombined sources	1
Modified residueⁱ	474	PhosphothreonineCombined sources	1
Modified residueⁱ	568	PhosphoserineCombined sources	1
Modified residueⁱ	729	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P14737
PaxDbⁱ	P14737
PRIDEⁱ	P14737

PTM databases

iPTMnetⁱ	P14737

iPTMnetⁱ

P14737

Interactionⁱ

Subunit structureⁱ

Physically associates with RAD53.

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CDC5	P32562	2	EBI-14788,EBI-4440
DPB11	P47027	13	EBI-14788,EBI-25984
RAD53	P22216	10	EBI-14788,EBI-17843

GO - Molecular functionⁱ

histone binding
Source: SGD
Inferred from direct assayⁱ
- 17721446

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	32270, 471 interactors
Database of interacting proteins More...DIPⁱ	DIP-2516N
ELMⁱ	P14737
IntActⁱ	P14737, 15 interactors
Molecular INTeraction database More...MINTⁱ	P14737
STRINGⁱ	4932.YDR217C

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Beta strandⁱ	190 – 192	Combined sources		3

Show more details

3D structure databases

ProteinModelPortalⁱ	P14737
SMRⁱ	P14737
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P14737

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	994 – 1122	BRCTPROSITE-ProRule annotationAdd BLAST		129

Phylogenomic databases

InParanoidⁱ	P14737
KEGG Orthology (KO) More...KOⁱ	K06661
OMAⁱ	ESKEWLI
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2JST

Family and domain databases

Gene3Dⁱ	3.40.50.10190, 1 hit
InterProⁱ	View protein in InterPro IPR001357 BRCT_dom IPR036420 BRCT_dom_sf IPR013914 Rad9_Rad53-bd_dom_fun
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00533 BRCT, 1 hit PF08605 Rad9_Rad53_bind, 1 hit
SMARTⁱ	View protein in SMART SM00292 BRCT, 1 hit
SUPFAMⁱ	SSF52113 SSF52113, 1 hit
PROSITEⁱ	View protein in PROSITE PS50172 BRCT, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P14737-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSGQLVQWKS SPDRVTQSAI KEALHSPLAD GDMNEMNVPV DPLENKVNST 
        60         70         80         90        100
NIIEGSPKAN PNPVKFMNTS EIFQKSLGLL DESPRHDDEL NIEVGDNDRP 
       110        120        130        140        150
NANILHNERT PDLDRIANFF KSNRTPGKEN LLTKYQSSDL EDTPLMLRKK 
       160        170        180        190        200
MTFQTPTDPL EQKTFKKLKS DTGFCYYGEQ NDGEENASLE VTEADATFVQ 
       210        220        230        240        250
MAERSADNYD CALEGIVTPK RYKDELSKSG GMQDERVQKT QIMISAESPN 
       260        270        280        290        300
SISSYDKNKI TGNGRTTRNV NKVFNNNEDN IGAIEEKNPV KKKSENYSSD 
       310        320        330        340        350
DLRERNNQII QSNESEEINE LEKNLNVSGR ENDVNNLDID INSAVSGTPS 
       360        370        380        390        400
RNNAEEEMYS SESVNNREPS KKWIFRYSKD KTENNSNRST QIVNNPRTQE 
       410        420        430        440        450
MPLDSISIDT QPLSKSFNTE TNNELETQII VSSLSQGISA QKGPVFHSTG 
       460        470        480        490        500
QTEEIKTQII NSPEQNALNA TFETPVTLSR INFEPILEVP ETSSPSKNTM 
       510        520        530        540        550
SKPSNSSPIP KEKDTFNIHE REVETNNVFS NDIQNSSNAA TRDDIIIAGS 
       560        570        580        590        600
SDFNEQKEIT DRIYLQLSGK QISDSGSDET ERMSPNELDT KKESTIMSEV 
       610        620        630        640        650
ELTQELPEVE EQQDLQTSPK KLVVEEETLM EIKKSKGNSL QLHDDNKECN 
       660        670        680        690        700
SDKQDGTESL DVALIEHESK GQSSELQKNL MQLFPSESQE IIQNRRTIKR 
       710        720        730        740        750
RQKDTIEIGE EEENRSTKTS PTKHLKRNSD LDAASIKREP SCSITIQTGE 
       760        770        780        790        800
TGSGKDSKEQ SYVFPEGIRT ADNSFLSKDD IIFGNAVWCQ YTWNYKFYPG 
       810        820        830        840        850
ILLEVDTNQD GCWIYFETGR SLTKDEDIYY LDIRIGDAVT FDGNEYVVVG 
       860        870        880        890        900
LECRSHDLNI IRCIRGYDTV HLKKKNASGL LGKRTLIKAL SSISLDLSEW 
       910        920        930        940        950
AKRAKIILED NEKNKGDAYR YLRHPIRGRK SMTNVLSPKK HTDDEKDINT 
       960        970        980        990       1000
HTEVYNNEIE SSSEKKEIVK KDSRDALAEH AGAPSLLFSS GEIRTGNVFD 
      1010       1020       1030       1040       1050
KCIFVLTSLF ENREELRQTI ESQGGTVIES GFSTLFNFTH PLAKSLVNKG 
      1060       1070       1080       1090       1100
NTDNIRELAL KLAWKPHSLF ADCRFACLIT KRHLRSLKYL ETLALGWPTL 
      1110       1120       1130       1140       1150
HWKFISACIE KKRIVPHLIY QYLLPSGESF RLSLDSPSKG GIIKSNNIFS 
      1160       1170       1180       1190       1200
FYTQFLRGSN LRDQICGVKK MLNDYIVIVW GRSELDSFVK FAFACLSAGR 
      1210       1220       1230       1240       1250
MLTIDLPNID VDDTEPLLNA LDSLVPRIGS ELSNRKLKFL IYANENNGKS 
      1260       1270       1280       1290       1300
QMKLLERLRS QISLKFKKFN YIFHTESKEW LIQTIINEDT GFHDDITDND 

IYNTISEVR

Length:1,309

Mass (Da):148,398

Last modified:October 5, 2010 - v2

Checksum:ⁱFCDAC16694484D8D

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	433	S → C in AAA34954 (PubMed:2664461).Curated		1
Sequence conflictⁱ	433	S → C no nucleotide entry (PubMed:2247073).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M26049 Genomic DNA Translation: AAA34954.1 Z48612 Genomic DNA Translation: CAA88497.1 BK006938 Genomic DNA Translation: DAA12060.1
PIRⁱ	S59424 BVBYD9
NCBI Reference Sequences More...RefSeqⁱ	NP_010503.1, NM_001180525.1

Genome annotation databases

EnsemblFungiⁱ	YDR217C; YDR217C; YDR217C
GeneIDⁱ	851803
KEGGⁱ	sce:YDR217C

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P14737	Rad9p		YEASC		1309	UniRef100_P14737

Protein	Similar proteins		Species	Score	Length	Source
P14737	Rad9p		YEASC		1309	UniRef90_P14737
Cell cycle arrest protein		YEAS1		1309
K7_Rad9p		YEASK		1309
Rad9p		YEAS8		1309
RAD9p DNA damage-dependent checkpoint protein		Saccharomyces sp. 'boulardii'		1309
+2

Protein	Similar proteins		Species	Score	Length	Source
P14737	Rad9p		YEASC		1309	UniRef50_P14737
Rad9p		YEAS2		1309
Cell cycle arrest protein		YEAS1		1309
Rad9p		YEAS8		1309
K7_Rad9p		YEASK		1309
+4

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M26049 Genomic DNA Translation: AAA34954.1 Z48612 Genomic DNA Translation: CAA88497.1 BK006938 Genomic DNA Translation: DAA12060.1
PIRⁱ	S59424 BVBYD9
RefSeqⁱ	NP_010503.1, NM_001180525.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1FHR	NMR	-	P	826-832	[»]
	1J4K	NMR	-	P	826-832	[»]
	1J4L	NMR	-	P	599-607	[»]
	1J4P	NMR	-	B	149-161	[»]
	1J4Q	NMR	-	B	188-200	[»]
	1K2M	NMR	-	P	826-832	[»]
	1K2N	NMR	-	P	599-607	[»]
	1K3N	NMR	-	B	149-161	[»]
	1K3Q	NMR	-	B	188-200	[»]
	2FF4	X-ray	1.90	E/F	188-195	[»]
ProteinModelPortalⁱ	P14737
SMRⁱ	P14737
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32270, 471 interactors
DIPⁱ	DIP-2516N
ELMⁱ	P14737
IntActⁱ	P14737, 15 interactors
MINTⁱ	P14737
STRINGⁱ	4932.YDR217C

PTM databases

iPTMnetⁱ	P14737

iPTMnetⁱ

P14737

Proteomic databases

MaxQBⁱ	P14737
PaxDbⁱ	P14737
PRIDEⁱ	P14737

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YDR217C; YDR217C; YDR217C
GeneIDⁱ	851803
KEGGⁱ	sce:YDR217C

Organism-specific databases

SGDⁱ	S000002625 RAD9

Phylogenomic databases

InParanoidⁱ	P14737
KOⁱ	K06661
OMAⁱ	ESKEWLI
OrthoDBⁱ	EOG092C2JST

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29798-MONOMER

BioCycⁱ

YEAST:G3O-29798-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P14737
Protein Ontology More...PROⁱ	PR:P14737

Family and domain databases

Gene3Dⁱ	3.40.50.10190, 1 hit
InterProⁱ	View protein in InterPro IPR001357 BRCT_dom IPR036420 BRCT_dom_sf IPR013914 Rad9_Rad53-bd_dom_fun
Pfamⁱ	View protein in Pfam PF00533 BRCT, 1 hit PF08605 Rad9_Rad53_bind, 1 hit
SMARTⁱ	View protein in SMART SM00292 BRCT, 1 hit
SUPFAMⁱ	SSF52113 SSF52113, 1 hit
PROSITEⁱ	View protein in PROSITE PS50172 BRCT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RAD9_YEAST
Accessionⁱ	P14737Primary (citable) accession number: P14737 Secondary accession number(s): D6VSK0, Q04920
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
	Last sequence update:	October 5, 2010
	Last modified:	November 7, 2018
	This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P14737 (RAD9_YEAST)

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DNA repair protein RAD9

RAD9

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ