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UniProtKB - P14737 (RAD9_YEAST)
Protein
DNA repair protein RAD9
Gene
RAD9
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Essential for cell cycle arrest at the G2 stage following DNA damage by X-irradiation or inactivation of DNA ligase.
Miscellaneous
Present with 400 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- double-stranded DNA binding Source: SGD
- enzyme activator activity Source: SGD
- histone binding Source: SGD
GO - Biological processi
- DNA damage checkpoint signaling Source: SGD
- DNA repair Source: SGD
- double-strand break repair Source: SGD
- mitotic G1 DNA damage checkpoint signaling Source: SGD
- mitotic intra-S DNA damage checkpoint signaling Source: SGD
- negative regulation of DNA strand resection involved in replication fork processing Source: SGD
- nucleotide-excision repair Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- regulation of cell cycle Source: SGD
Keywordsi
Molecular function | DNA replication inhibitor |
Biological process | Cell cycle, DNA damage |
Enzyme and pathway databases
Reactomei | R-SCE-3232118, SUMOylation of transcription factors R-SCE-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
Names & Taxonomyi
Protein namesi | Recommended name: DNA repair protein RAD9 |
Gene namesi | Name:RAD9 Ordered Locus Names:YDR217C ORF Names:YD9934.02C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002625, RAD9 |
VEuPathDBi | FungiDB:YDR217C |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097158 | 1 – 1309 | DNA repair protein RAD9Add BLAST | 1309 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 26 | PhosphoserineCombined sources | 1 | |
Modified residuei | 56 | PhosphoserineCombined sources | 1 | |
Modified residuei | 205 | PhosphoserineCombined sources | 1 | |
Modified residuei | 218 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 248 | PhosphoserineCombined sources | 1 | |
Modified residuei | 312 | PhosphoserineCombined sources | 1 | |
Modified residuei | 315 | PhosphoserineCombined sources | 1 | |
Modified residuei | 462 | PhosphoserineCombined sources | 1 | |
Modified residuei | 471 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 474 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 568 | PhosphoserineCombined sources | 1 | |
Modified residuei | 729 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P14737 |
PaxDbi | P14737 |
PRIDEi | P14737 |
PTM databases
iPTMneti | P14737 |
Interactioni
Subunit structurei
Physically associates with RAD53.
Binary interactionsi
P14737
With | #Exp. | IntAct |
---|---|---|
CDC5 [P32562] | 2 | EBI-14788,EBI-4440 |
DPB11 [P47027] | 13 | EBI-14788,EBI-25984 |
RAD53 [P22216] | 12 | EBI-14788,EBI-17843 |
GO - Molecular functioni
- histone binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 32270, 481 interactors |
DIPi | DIP-2516N |
ELMi | P14737 |
IntActi | P14737, 15 interactors |
MINTi | P14737 |
STRINGi | 4932.YDR217C |
Miscellaneous databases
RNActi | P14737, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P14737 |
SMRi | P14737 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14737 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 994 – 1122 | BRCTPROSITE-ProRule annotationAdd BLAST | 129 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 280 – 299 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 342 – 365 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 490 – 512 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 636 – 655 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 691 – 731 | DisorderedSequence analysisAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 17 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 284 – 299 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 693 – 731 | Basic and acidic residuesSequence analysisAdd BLAST | 39 |
Phylogenomic databases
eggNOGi | KOG3548, Eukaryota |
HOGENOMi | CLU_279536_0_0_1 |
InParanoidi | P14737 |
OMAi | FDAMADV |
Family and domain databases
Gene3Di | 3.40.50.10190, 1 hit |
IDEALi | IID50198 |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR013914, Rad9_Rad53-bd_dom_fun |
Pfami | View protein in Pfam PF00533, BRCT, 1 hit PF08605, Rad9_Rad53_bind, 1 hit |
SMARTi | View protein in SMART SM00292, BRCT, 1 hit |
SUPFAMi | SSF52113, SSF52113, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit |
i Sequence
Sequence statusi: Complete.
P14737-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGQLVQWKS SPDRVTQSAI KEALHSPLAD GDMNEMNVPV DPLENKVNST
60 70 80 90 100
NIIEGSPKAN PNPVKFMNTS EIFQKSLGLL DESPRHDDEL NIEVGDNDRP
110 120 130 140 150
NANILHNERT PDLDRIANFF KSNRTPGKEN LLTKYQSSDL EDTPLMLRKK
160 170 180 190 200
MTFQTPTDPL EQKTFKKLKS DTGFCYYGEQ NDGEENASLE VTEADATFVQ
210 220 230 240 250
MAERSADNYD CALEGIVTPK RYKDELSKSG GMQDERVQKT QIMISAESPN
260 270 280 290 300
SISSYDKNKI TGNGRTTRNV NKVFNNNEDN IGAIEEKNPV KKKSENYSSD
310 320 330 340 350
DLRERNNQII QSNESEEINE LEKNLNVSGR ENDVNNLDID INSAVSGTPS
360 370 380 390 400
RNNAEEEMYS SESVNNREPS KKWIFRYSKD KTENNSNRST QIVNNPRTQE
410 420 430 440 450
MPLDSISIDT QPLSKSFNTE TNNELETQII VSSLSQGISA QKGPVFHSTG
460 470 480 490 500
QTEEIKTQII NSPEQNALNA TFETPVTLSR INFEPILEVP ETSSPSKNTM
510 520 530 540 550
SKPSNSSPIP KEKDTFNIHE REVETNNVFS NDIQNSSNAA TRDDIIIAGS
560 570 580 590 600
SDFNEQKEIT DRIYLQLSGK QISDSGSDET ERMSPNELDT KKESTIMSEV
610 620 630 640 650
ELTQELPEVE EQQDLQTSPK KLVVEEETLM EIKKSKGNSL QLHDDNKECN
660 670 680 690 700
SDKQDGTESL DVALIEHESK GQSSELQKNL MQLFPSESQE IIQNRRTIKR
710 720 730 740 750
RQKDTIEIGE EEENRSTKTS PTKHLKRNSD LDAASIKREP SCSITIQTGE
760 770 780 790 800
TGSGKDSKEQ SYVFPEGIRT ADNSFLSKDD IIFGNAVWCQ YTWNYKFYPG
810 820 830 840 850
ILLEVDTNQD GCWIYFETGR SLTKDEDIYY LDIRIGDAVT FDGNEYVVVG
860 870 880 890 900
LECRSHDLNI IRCIRGYDTV HLKKKNASGL LGKRTLIKAL SSISLDLSEW
910 920 930 940 950
AKRAKIILED NEKNKGDAYR YLRHPIRGRK SMTNVLSPKK HTDDEKDINT
960 970 980 990 1000
HTEVYNNEIE SSSEKKEIVK KDSRDALAEH AGAPSLLFSS GEIRTGNVFD
1010 1020 1030 1040 1050
KCIFVLTSLF ENREELRQTI ESQGGTVIES GFSTLFNFTH PLAKSLVNKG
1060 1070 1080 1090 1100
NTDNIRELAL KLAWKPHSLF ADCRFACLIT KRHLRSLKYL ETLALGWPTL
1110 1120 1130 1140 1150
HWKFISACIE KKRIVPHLIY QYLLPSGESF RLSLDSPSKG GIIKSNNIFS
1160 1170 1180 1190 1200
FYTQFLRGSN LRDQICGVKK MLNDYIVIVW GRSELDSFVK FAFACLSAGR
1210 1220 1230 1240 1250
MLTIDLPNID VDDTEPLLNA LDSLVPRIGS ELSNRKLKFL IYANENNGKS
1260 1270 1280 1290 1300
QMKLLERLRS QISLKFKKFN YIFHTESKEW LIQTIINEDT GFHDDITDND
IYNTISEVR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 433 | S → C in AAA34954 (PubMed:2664461).Curated | 1 | |
Sequence conflicti | 433 | S → C no nucleotide entry (PubMed:2247073).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26049 Genomic DNA Translation: AAA34954.1 Z48612 Genomic DNA Translation: CAA88497.1 BK006938 Genomic DNA Translation: DAA12060.1 |
PIRi | S59424, BVBYD9 |
RefSeqi | NP_010503.1, NM_001180525.1 |
Genome annotation databases
EnsemblFungii | YDR217C_mRNA; YDR217C; YDR217C |
GeneIDi | 851803 |
KEGGi | sce:YDR217C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26049 Genomic DNA Translation: AAA34954.1 Z48612 Genomic DNA Translation: CAA88497.1 BK006938 Genomic DNA Translation: DAA12060.1 |
PIRi | S59424, BVBYD9 |
RefSeqi | NP_010503.1, NM_001180525.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FHR | NMR | - | P | 826-832 | [»] | |
1J4K | NMR | - | P | 826-832 | [»] | |
1J4L | NMR | - | P | 599-607 | [»] | |
1J4P | NMR | - | B | 149-161 | [»] | |
1J4Q | NMR | - | B | 188-200 | [»] | |
1K2M | NMR | - | P | 826-832 | [»] | |
1K2N | NMR | - | P | 599-607 | [»] | |
1K3N | NMR | - | B | 149-161 | [»] | |
1K3Q | NMR | - | B | 188-200 | [»] | |
2FF4 | X-ray | 1.90 | E/F | 188-195 | [»] | |
AlphaFoldDBi | P14737 | |||||
SMRi | P14737 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32270, 481 interactors |
DIPi | DIP-2516N |
ELMi | P14737 |
IntActi | P14737, 15 interactors |
MINTi | P14737 |
STRINGi | 4932.YDR217C |
PTM databases
iPTMneti | P14737 |
Proteomic databases
MaxQBi | P14737 |
PaxDbi | P14737 |
PRIDEi | P14737 |
Genome annotation databases
EnsemblFungii | YDR217C_mRNA; YDR217C; YDR217C |
GeneIDi | 851803 |
KEGGi | sce:YDR217C |
Organism-specific databases
SGDi | S000002625, RAD9 |
VEuPathDBi | FungiDB:YDR217C |
Phylogenomic databases
eggNOGi | KOG3548, Eukaryota |
HOGENOMi | CLU_279536_0_0_1 |
InParanoidi | P14737 |
OMAi | FDAMADV |
Enzyme and pathway databases
Reactomei | R-SCE-3232118, SUMOylation of transcription factors R-SCE-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
Miscellaneous databases
EvolutionaryTracei | P14737 |
PROi | PR:P14737 |
RNActi | P14737, protein |
Family and domain databases
Gene3Di | 3.40.50.10190, 1 hit |
IDEALi | IID50198 |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR013914, Rad9_Rad53-bd_dom_fun |
Pfami | View protein in Pfam PF00533, BRCT, 1 hit PF08605, Rad9_Rad53_bind, 1 hit |
SMARTi | View protein in SMART SM00292, BRCT, 1 hit |
SUPFAMi | SSF52113, SSF52113, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD9_YEAST | |
Accessioni | P14737Primary (citable) accession number: P14737 Secondary accession number(s): D6VSK0, Q04920 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | October 5, 2010 | |
Last modified: | May 25, 2022 | |
This is version 194 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references