DNA repair protein RAD9

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• double-stranded DNA binding Source: SGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA."
    Lancelot N., Charier G., Couprie J., Duband-Goulet I., Alpha-Bazin B., Quemeneur E., Ma E., Marsolier-Kergoat M.C., Ropars V., Charbonnier J.B., Miron S., Craescu C.T., Callebaut I., Gilquin B., Zinn-Justin S.
    Nucleic Acids Res 35:5898-5912(2007) [PubMed] [Europe PMC] [Abstract]
• enzyme activator activity Source: SGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Use of quantitative mass spectrometric analysis to elucidate the mechanisms of phospho-priming and auto-activation of the checkpoint kinase Rad53 in vivo."
    Chen E.S., Hoch N.C., Wang S.C., Pellicioli A., Heierhorst J., Tsai M.D.
    Mol Cell Proteomics 13:551-565(2014) [PubMed] [Europe PMC] [Abstract]
• histone binding Source: SGDInferred from direct assayⁱ
  • "Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast."
    Hammet A., Magill C., Heierhorst J., Jackson S.P.
    EMBO Rep 8:851-857(2007) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• DNA damage checkpoint signaling Source: SGDInferred from mutant phenotypeⁱ
  • "RAD9 and RAD24 define two additive, interacting branches of the DNA damage checkpoint pathway in budding yeast normally required for Rad53 modification and activation."
    de la Torre-Ruiz M.A., Green C.M., Lowndes N.F.
    EMBO J 17:2687-2698(1998) [PubMed] [Europe PMC] [Abstract]
• DNA repair Source: SGDInferred from mutant phenotypeⁱ
  • Ref.1

    "Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage."
    Schiestl R.H., Reynolds P., Prakash S., Prakash L.
    Mol. Cell. Biol. 9:1882-1896(1989) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• double-strand break repair Source: SGDInferred from mutant phenotypeⁱ
  • "Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks."
    Dibitetto D., Ferrari M., Rawal C.C., Balint A., Kim T., Zhang Z., Smolka M.B., Brown G.W., Marini F., Pellicioli A.
    Nucleic Acids Res 44:669-682(2016) [PubMed] [Europe PMC] [Abstract]
• mitotic G1 DNA damage checkpoint signaling Source: SGDInferred from mutant phenotypeⁱ
  • "Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9."
    Wysocki R., Javaheri A., Allard S., Sha F., Cote J., Kron S.J.
    Mol. Cell. Biol. 25:8430-8443(2005) [PubMed] [Europe PMC] [Abstract]
  • "Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast."
    Hammet A., Magill C., Heierhorst J., Jackson S.P.
    EMBO Rep 8:851-857(2007) [PubMed] [Europe PMC] [Abstract]
• mitotic intra-S DNA damage checkpoint signaling Source: SGDInferred from mutant phenotypeⁱ
  • "Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9."
    Wysocki R., Javaheri A., Allard S., Sha F., Cote J., Kron S.J.
    Mol. Cell. Biol. 25:8430-8443(2005) [PubMed] [Europe PMC] [Abstract]
  • "Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast."
    Hammet A., Magill C., Heierhorst J., Jackson S.P.
    EMBO Rep 8:851-857(2007) [PubMed] [Europe PMC] [Abstract]
• negative regulation of DNA strand resection involved in replication fork processing Source: SGDInferred from mutant phenotypeⁱ
  • "Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection."
    Bonetti D., Villa M., Gobbini E., Cassani C., Tedeschi G., Longhese M.P.
    EMBO Rep 16:351-361(2015) [PubMed] [Europe PMC] [Abstract]
• nucleotide-excision repair Source: SGDInferred from mutant phenotypeⁱ
  • "RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene."
    Yu S., Teng Y., Lowndes N.F., Waters R.
    Mutat Res 485:229-236(2001) [PubMed] [Europe PMC] [Abstract]
• positive regulation of transcription by RNA polymerase II Source: SGDInferred from mutant phenotypeⁱ
  • "A novel role for the budding yeast RAD9 checkpoint gene in DNA damage-dependent transcription."
    Aboussekhra A., Vialard J.E., Morrison D.E., de la Torre-Ruiz M.A., Cernakova L., Fabre F., Lowndes N.F.
    EMBO J 15:3912-3922(1996) [PubMed] [Europe PMC] [Abstract]
• regulation of cell cycle Source: SGDInferred from mutant phenotypeⁱ
  • Ref.1

    "Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage."
    Schiestl R.H., Reynolds P., Prakash S., Prakash L.
    Mol. Cell. Biol. 9:1882-1896(1989) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

• UniProt annotation
• GO - Cellular component

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• histone binding Source: SGDInferred from direct assayⁱ
  • "Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast."
    Hammet A., Magill C., Heierhorst J., Jackson S.P.
    EMBO Rep 8:851-857(2007) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

Region

Compositional bias

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MSGQLVQWKS SPDRVTQSAI KEALHSPLAD GDMNEMNVPV DPLENKVNST 
        60         70         80         90        100
NIIEGSPKAN PNPVKFMNTS EIFQKSLGLL DESPRHDDEL NIEVGDNDRP 
       110        120        130        140        150
NANILHNERT PDLDRIANFF KSNRTPGKEN LLTKYQSSDL EDTPLMLRKK 
       160        170        180        190        200
MTFQTPTDPL EQKTFKKLKS DTGFCYYGEQ NDGEENASLE VTEADATFVQ 
       210        220        230        240        250
MAERSADNYD CALEGIVTPK RYKDELSKSG GMQDERVQKT QIMISAESPN 
       260        270        280        290        300
SISSYDKNKI TGNGRTTRNV NKVFNNNEDN IGAIEEKNPV KKKSENYSSD 
       310        320        330        340        350
DLRERNNQII QSNESEEINE LEKNLNVSGR ENDVNNLDID INSAVSGTPS 
       360        370        380        390        400
RNNAEEEMYS SESVNNREPS KKWIFRYSKD KTENNSNRST QIVNNPRTQE 
       410        420        430        440        450
MPLDSISIDT QPLSKSFNTE TNNELETQII VSSLSQGISA QKGPVFHSTG 
       460        470        480        490        500
QTEEIKTQII NSPEQNALNA TFETPVTLSR INFEPILEVP ETSSPSKNTM 
       510        520        530        540        550
SKPSNSSPIP KEKDTFNIHE REVETNNVFS NDIQNSSNAA TRDDIIIAGS 
       560        570        580        590        600
SDFNEQKEIT DRIYLQLSGK QISDSGSDET ERMSPNELDT KKESTIMSEV 
       610        620        630        640        650
ELTQELPEVE EQQDLQTSPK KLVVEEETLM EIKKSKGNSL QLHDDNKECN 
       660        670        680        690        700
SDKQDGTESL DVALIEHESK GQSSELQKNL MQLFPSESQE IIQNRRTIKR 
       710        720        730        740        750
RQKDTIEIGE EEENRSTKTS PTKHLKRNSD LDAASIKREP SCSITIQTGE 
       760        770        780        790        800
TGSGKDSKEQ SYVFPEGIRT ADNSFLSKDD IIFGNAVWCQ YTWNYKFYPG 
       810        820        830        840        850
ILLEVDTNQD GCWIYFETGR SLTKDEDIYY LDIRIGDAVT FDGNEYVVVG 
       860        870        880        890        900
LECRSHDLNI IRCIRGYDTV HLKKKNASGL LGKRTLIKAL SSISLDLSEW 
       910        920        930        940        950
AKRAKIILED NEKNKGDAYR YLRHPIRGRK SMTNVLSPKK HTDDEKDINT 
       960        970        980        990       1000
HTEVYNNEIE SSSEKKEIVK KDSRDALAEH AGAPSLLFSS GEIRTGNVFD 
      1010       1020       1030       1040       1050
KCIFVLTSLF ENREELRQTI ESQGGTVIES GFSTLFNFTH PLAKSLVNKG 
      1060       1070       1080       1090       1100
NTDNIRELAL KLAWKPHSLF ADCRFACLIT KRHLRSLKYL ETLALGWPTL 
      1110       1120       1130       1140       1150
HWKFISACIE KKRIVPHLIY QYLLPSGESF RLSLDSPSKG GIIKSNNIFS 
      1160       1170       1180       1190       1200
FYTQFLRGSN LRDQICGVKK MLNDYIVIVW GRSELDSFVK FAFACLSAGR 
      1210       1220       1230       1240       1250
MLTIDLPNID VDDTEPLLNA LDSLVPRIGS ELSNRKLKFL IYANENNGKS 
      1260       1270       1280       1290       1300
QMKLLERLRS QISLKFKKFN YIFHTESKEW LIQTIINEDT GFHDDITDND 

IYNTISEVR                                              

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Yeast
   Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
2. Yeast chromosome IV
   Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
3. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references