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Protein

DNA repair protein RAD4

Gene

RAD4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents.

Miscellaneous

Present with 876 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi250 – 269Sequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-30323-MONOMER
ReactomeiR-SCE-3108214 SUMOylation of DNA damage response and repair proteins
R-SCE-5696394 DNA Damage Recognition in GG-NER
R-SCE-5696395 Formation of Incision Complex in GG-NER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD4
Gene namesi
Name:RAD4
Ordered Locus Names:YER162C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER162C
SGDiS000000964 RAD4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002182981 – 754DNA repair protein RAD4Add BLAST754

Proteomic databases

MaxQBiP14736
PaxDbiP14736
PRIDEiP14736

Interactioni

Binary interactionsi

Protein-protein interaction databases

BioGridi36915, 434 interactors
ComplexPortaliCPX-1640 Nucleotide excision repair factor 2 complex
DIPiDIP-1547N
IntActiP14736, 6 interactors
MINTiP14736
STRINGi4932.YER162C

Structurei

Secondary structure

1754
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP14736
SMRiP14736
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14736

Family & Domainsi

Sequence similaritiesi

Belongs to the XPC family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000005194
HOGENOMiHOG000074544
InParanoidiP14736
KOiK10838
OMAiAREPMPR
OrthoDBiEOG092C3500

Family and domain databases

Gene3Di3.90.260.10, 1 hit
InterProiView protein in InterPro
IPR018327 BHD_2
IPR004583 DNA_repair_Rad4
IPR038765 Papain_like_cys_pep_sf
IPR018325 Rad4/PNGase_transGLS-fold
IPR018326 Rad4_beta-hairpin_dom1
IPR018328 Rad4_beta-hairpin_dom3
IPR036985 Transglutaminase-like_sf
PANTHERiPTHR12135 PTHR12135, 2 hits
PfamiView protein in Pfam
PF10403 BHD_1, 1 hit
PF10405 BHD_3, 1 hit
PF03835 Rad4, 1 hit
SMARTiView protein in SMART
SM01030 BHD_1, 1 hit
SM01031 BHD_2, 1 hit
SM01032 BHD_3, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit

Sequencei

Sequence statusi: Complete.

P14736-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNEDLPKEYF ELIRKALNEK EAEKAPLSRR RRVRRKNQPL PDAKKKFKTG
60 70 80 90 100
LNELPRESVV TVNLDSSDDG VVTVPTDDSV EEIQSSEEDY DSEEFEDVTD
110 120 130 140 150
GNEVAGVEDI SVEIKPSSKR NSDARRTSRN VCSNEERKRR KYFHMLYLVC
160 170 180 190 200
LMVHGFIRNE WINSKRLSRK LSNLVPEKVF ELLHPQKDEE LPLRSTRKLL
210 220 230 240 250
DGLKKCMELW QKHWKITKKY DNVGLYMRTW KEIEMSANNK RKFKTLKRSD
260 270 280 290 300
FLRAVSKGHG DPDISVQGFV AMLRACNVNA RLIMSCQPPD FTNMKIDTSL
310 320 330 340 350
NGNNAYKDMV KYPIFWCEVW DKFSKKWITV DPVNLKTIEQ VRLHSKLAPK
360 370 380 390 400
GVACCERNML RYVIAYDRKY GCRDVTRRYA QWMNSKVRKR RITKDDFGEK
410 420 430 440 450
WFRKVITALH HRKRTKIDDY EDQYFFQRDE SEGIPDSVQD LKNHPYYVLE
460 470 480 490 500
QDIKQTQIVK PGCKECGYLK VHGKVGKVLK VYAKRDIADL KSARQWYMNG
510 520 530 540 550
RILKTGSRCK KVIKRTVGRP KGEAEEEDER LYSFEDTELY IPPLASASGE
560 570 580 590 600
ITKNTFGNIE VFAPTMIPGN CCLVENPVAI KAARFLGVEF APAVTSFKFE
610 620 630 640 650
RGSTVKPVLS GIVVAKWLRE AIETAIDGIE FIQEDDNRKE HLLGALESWN
660 670 680 690 700
TLLLKLRIRS KLNSTYGKIA EEEPNVTKEQ NIADNHDNTE TFMGGGFLPG
710 720 730 740 750
IANHEARPYS EPSEPEDSLD YVSVDKAEES ATDDDVGEDY SDFMKELEMS

EESD
Length:754
Mass (Da):87,174
Last modified:September 21, 2011 - v3
Checksum:i788D3B6F580D2BF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223V → E in AAB64689 (PubMed:9169868).Curated1
Sequence conflicti225L → I in AAA34944 (PubMed:3073107).Curated1
Sequence conflicti225L → I in AAA34945 (PubMed:2649477).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26050 Genomic DNA Translation: AAA34944.1
M24928 Genomic DNA Translation: AAA34945.1
U18917 Genomic DNA Translation: AAB64689.1
BK006939 Genomic DNA Translation: DAA07824.2
PIRiS30814 DDBYD4
RefSeqiNP_011089.4, NM_001179052.4

Genome annotation databases

EnsemblFungiiYER162C; YER162C; YER162C
GeneIDi856909
KEGGisce:YER162C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26050 Genomic DNA Translation: AAA34944.1
M24928 Genomic DNA Translation: AAA34945.1
U18917 Genomic DNA Translation: AAB64689.1
BK006939 Genomic DNA Translation: DAA07824.2
PIRiS30814 DDBYD4
RefSeqiNP_011089.4, NM_001179052.4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2M14NMR-B76-115[»]
2QSFX-ray2.35A101-632[»]
2QSGX-ray3.10A101-632[»]
2QSHX-ray2.81A101-632[»]
4YIRX-ray3.05A101-632[»]
ProteinModelPortaliP14736
SMRiP14736
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36915, 434 interactors
ComplexPortaliCPX-1640 Nucleotide excision repair factor 2 complex
DIPiDIP-1547N
IntActiP14736, 6 interactors
MINTiP14736
STRINGi4932.YER162C

Proteomic databases

MaxQBiP14736
PaxDbiP14736
PRIDEiP14736

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER162C; YER162C; YER162C
GeneIDi856909
KEGGisce:YER162C

Organism-specific databases

EuPathDBiFungiDB:YER162C
SGDiS000000964 RAD4

Phylogenomic databases

GeneTreeiENSGT00390000005194
HOGENOMiHOG000074544
InParanoidiP14736
KOiK10838
OMAiAREPMPR
OrthoDBiEOG092C3500

Enzyme and pathway databases

BioCyciYEAST:G3O-30323-MONOMER
ReactomeiR-SCE-3108214 SUMOylation of DNA damage response and repair proteins
R-SCE-5696394 DNA Damage Recognition in GG-NER
R-SCE-5696395 Formation of Incision Complex in GG-NER

Miscellaneous databases

EvolutionaryTraceiP14736
PROiPR:P14736

Family and domain databases

Gene3Di3.90.260.10, 1 hit
InterProiView protein in InterPro
IPR018327 BHD_2
IPR004583 DNA_repair_Rad4
IPR038765 Papain_like_cys_pep_sf
IPR018325 Rad4/PNGase_transGLS-fold
IPR018326 Rad4_beta-hairpin_dom1
IPR018328 Rad4_beta-hairpin_dom3
IPR036985 Transglutaminase-like_sf
PANTHERiPTHR12135 PTHR12135, 2 hits
PfamiView protein in Pfam
PF10403 BHD_1, 1 hit
PF10405 BHD_3, 1 hit
PF03835 Rad4, 1 hit
SMARTiView protein in SMART
SM01030 BHD_1, 1 hit
SM01031 BHD_2, 1 hit
SM01032 BHD_3, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAD4_YEAST
AccessioniPrimary (citable) accession number: P14736
Secondary accession number(s): D3DM70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: September 21, 2011
Last modified: September 12, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names
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