UniProtKB - P14733 (LMNB1_MOUSE)
Protein
Lamin-B1
Gene
Lmnb1
Organism
Mus musculus (Mouse)
Status
Functioni
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Miscellaneous
The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.
GO - Molecular functioni
- double-stranded DNA binding Source: MGI
- JUN kinase binding Source: BHF-UCL
- phospholipase binding Source: BHF-UCL
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cellular response to monosodium L-glutamate Source: MGI
- positive regulation of G2/M transition of mitotic cell cycle Source: BHF-UCL
- positive regulation of JNK cascade Source: BHF-UCL
Enzyme and pathway databases
Reactomei | R-MMU-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) R-MMU-2980766, Nuclear Envelope Breakdown R-MMU-2995383, Initiation of Nuclear Envelope (NE) Reformation R-MMU-352238, Breakdown of the nuclear lamina R-MMU-4419969, Depolymerisation of the Nuclear Lamina |
Names & Taxonomyi
Protein namesi | Recommended name: Lamin-B1 |
Gene namesi | Name:Lmnb1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96795, Lmnb1 |
Subcellular locationi
Nucleus
Cytoskeleton
- lamin filament Source: MGI
Nucleus
- lamin filament Source: MGI
- nuclear envelope Source: BHF-UCL
- nuclear inner membrane Source: MGI
- nuclear lamina Source: MGI
- nuclear matrix Source: MGI
- nuclear membrane Source: MGI
- nucleoplasm Source: BHF-UCL
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Intermediate filament, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000063817 | 2 – 585 | Lamin-B1Add BLAST | 584 | |
PropeptideiPRO_0000403467 | 586 – 588 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 3 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 5 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 15 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 17 | PhosphoserineCombined sources | 1 | |
Modified residuei | 21 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 24 | PhosphoserineCombined sources | 1 | |
Modified residuei | 26 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 29 | PhosphoserineBy similarity | 1 | |
Cross-linki | 103 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 112 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 124 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 127 | PhosphoserineBy similarity | 1 | |
Cross-linki | 146 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 158 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 158 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 159 | PhosphoserineBy similarity | 1 | |
Cross-linki | 182 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 201 | PhosphoserineBy similarity | 1 | |
Modified residuei | 233 | PhosphoserineBy similarity | 1 | |
Cross-linki | 242 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 262 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 272 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 272 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 279 | PhosphoserineBy similarity | 1 | |
Modified residuei | 303 | PhosphoserineBy similarity | 1 | |
Cross-linki | 313 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Disulfide bondi | 318 | InterchainBy similarity | ||
Modified residuei | 331 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 331 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 376 | PhosphoserineBy similarity | 1 | |
Modified residuei | 414 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 484 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 533 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 535 | PhosphoserineBy similarity | 1 | |
Cross-linki | 548 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 585 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 585 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Keywords - PTMi
Acetylation, Disulfide bond, Isopeptide bond, Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugationProteomic databases
EPDi | P14733 |
jPOSTi | P14733 |
MaxQBi | P14733 |
PaxDbi | P14733 |
PeptideAtlasi | P14733 |
PRIDEi | P14733 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00230394 |
SWISS-2DPAGEi | P14733 |
PTM databases
iPTMneti | P14733 |
PhosphoSitePlusi | P14733 |
SwissPalmi | P14733 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024590, Expressed in cumulus cell and 271 other tissues |
Genevisiblei | P14733, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts with lamin-associated polypeptides IA, IB and 2.
Interacts with SPAG4 and SEPT12 (By similarity).
By similarityGO - Molecular functioni
- JUN kinase binding Source: BHF-UCL
- phospholipase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 201177, 20 interactors |
IntActi | P14733, 17 interactors |
MINTi | P14733 |
STRINGi | 10090.ENSMUSP00000025486 |
Miscellaneous databases
RNActi | P14733, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 389 | IF rodPROSITE-ProRule annotationAdd BLAST | 357 | |
Domaini | 431 – 547 | LTDPROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 35 | HeadAdd BLAST | 34 | |
Regioni | 36 – 70 | Coil 1AAdd BLAST | 35 | |
Regioni | 71 – 82 | Linker 1Add BLAST | 12 | |
Regioni | 83 – 216 | Coil 1BAdd BLAST | 134 | |
Regioni | 217 – 244 | Linker 2Add BLAST | 28 | |
Regioni | 245 – 387 | Coil 2Add BLAST | 143 | |
Regioni | 388 – 588 | TailAdd BLAST | 201 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 416 – 421 | Nuclear localization signalSequence analysis | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 553 – 561 | Glu-rich (highly acidic; could be involved in chromatin binding) | 9 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0977, Eukaryota |
GeneTreei | ENSGT00940000157199 |
HOGENOMi | CLU_012560_9_2_1 |
InParanoidi | P14733 |
OMAi | ERAWLQI |
OrthoDBi | 701388at2759 |
PhylomeDBi | P14733 |
TreeFami | TF101181 |
Family and domain databases
Gene3Di | 1.20.5.1160, 2 hits 2.60.40.1260, 1 hit |
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR001322, Lamin_tail_dom IPR036415, Lamin_tail_dom_sf |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF00932, LTD, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
SUPFAMi | SSF74853, SSF74853, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit PS51841, LTD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P14733-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATATPVQQQ RAGSRASAPA TPLSPTRLSR LQEKEELREL NDRLAVYIDK
60 70 80 90 100
VRSLETENSA LQLQVTEREE VRGRELTGLK ALYETELADA RRALDDTARE
110 120 130 140 150
RAKLQIELGK FKAEHDQLLL NYAKKESDLS GAQIKLREYE AALNSKDAAL
160 170 180 190 200
ATALGDKKSL EGDLEDLKDQ IAQLEASLSA AKKQLADETL LKVDLENRCQ
210 220 230 240 250
SLTEDLEFRK NMYEEEINET RRKHETRLVE VDSGRQIEYE YKLAQALHEM
260 270 280 290 300
REQHDAQVRL YKEELEQTYH AKLENARLSS EMNTSTVNSA REELMESRMR
310 320 330 340 350
IESLSSQLSN LQKESRACLE RIQELEDMLA KERDNSRRML SDREREMAEI
360 370 380 390 400
RDQMQQQLSD YEQLLDVKLA LDMEISAYRK LLEGEEERLK LSPSPSSRVT
410 420 430 440 450
VSRASSSRSV RTTRGKRKRV DVEESEASSS VSISHSASAT GNVCIEEIDV
460 470 480 490 500
DGKFIRLKNT SEQDQPMGGW EMIRKIGDTS VSYKYTSRYV LKAGQTVTVW
510 520 530 540 550
AANAGVTASP PTDLIWKNQN SWGTGEDVKV ILKNSQGEEV AQRSTVFKTT
560 570 580
IPEEEEEEEE EPIGVAVEEE RFHQQGAPRA SNKSCAIM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 581 | S → W in CAA34677 (PubMed:3243285).Curated | 1 | |
Sequence conflicti | 581 | S → W in AAC96023 (PubMed:3243285).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16705 mRNA Translation: CAA34677.1 M35153 mRNA Translation: AAC96023.1 Sequence problems. D50080 Genomic DNA Translation: BAA08784.1 BC052729 mRNA Translation: AAH52729.1 BC058392 mRNA Translation: AAH58392.1 |
CCDSi | CCDS29261.1 |
PIRi | S07720 |
RefSeqi | NP_034851.2, NM_010721.2 |
Genome annotation databases
Ensembli | ENSMUST00000025486; ENSMUSP00000025486; ENSMUSG00000024590 |
GeneIDi | 16906 |
KEGGi | mmu:16906 |
UCSCi | uc012bdd.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16705 mRNA Translation: CAA34677.1 M35153 mRNA Translation: AAC96023.1 Sequence problems. D50080 Genomic DNA Translation: BAA08784.1 BC052729 mRNA Translation: AAH52729.1 BC058392 mRNA Translation: AAH58392.1 |
CCDSi | CCDS29261.1 |
PIRi | S07720 |
RefSeqi | NP_034851.2, NM_010721.2 |
3D structure databases
BMRBi | P14733 |
SMRi | P14733 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201177, 20 interactors |
IntActi | P14733, 17 interactors |
MINTi | P14733 |
STRINGi | 10090.ENSMUSP00000025486 |
PTM databases
iPTMneti | P14733 |
PhosphoSitePlusi | P14733 |
SwissPalmi | P14733 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00230394 |
SWISS-2DPAGEi | P14733 |
Proteomic databases
EPDi | P14733 |
jPOSTi | P14733 |
MaxQBi | P14733 |
PaxDbi | P14733 |
PeptideAtlasi | P14733 |
PRIDEi | P14733 |
Protocols and materials databases
Antibodypediai | 3937, 768 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025486; ENSMUSP00000025486; ENSMUSG00000024590 |
GeneIDi | 16906 |
KEGGi | mmu:16906 |
UCSCi | uc012bdd.2, mouse |
Organism-specific databases
CTDi | 4001 |
MGIi | MGI:96795, Lmnb1 |
Phylogenomic databases
eggNOGi | KOG0977, Eukaryota |
GeneTreei | ENSGT00940000157199 |
HOGENOMi | CLU_012560_9_2_1 |
InParanoidi | P14733 |
OMAi | ERAWLQI |
OrthoDBi | 701388at2759 |
PhylomeDBi | P14733 |
TreeFami | TF101181 |
Enzyme and pathway databases
Reactomei | R-MMU-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) R-MMU-2980766, Nuclear Envelope Breakdown R-MMU-2995383, Initiation of Nuclear Envelope (NE) Reformation R-MMU-352238, Breakdown of the nuclear lamina R-MMU-4419969, Depolymerisation of the Nuclear Lamina |
Miscellaneous databases
BioGRID-ORCSi | 16906, 4 hits in 19 CRISPR screens |
ChiTaRSi | Lmnb1, mouse |
PROi | PR:P14733 |
RNActi | P14733, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024590, Expressed in cumulus cell and 271 other tissues |
Genevisiblei | P14733, MM |
Family and domain databases
Gene3Di | 1.20.5.1160, 2 hits 2.60.40.1260, 1 hit |
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR001322, Lamin_tail_dom IPR036415, Lamin_tail_dom_sf |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF00932, LTD, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
SUPFAMi | SSF74853, SSF74853, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit PS51841, LTD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LMNB1_MOUSE | |
Accessioni | P14733Primary (citable) accession number: P14733 Secondary accession number(s): Q61791 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 199 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families