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Protein

26S proteasome non-ATPase regulatory subunit 3

Gene

Psmd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTumor antigen

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-MMU-350562 Regulation of ornithine decarboxylase (ODC)
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-4641257 Degradation of AXIN
R-MMU-4641258 Degradation of DVL
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5632684 Hedgehog 'on' state
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5687128 MAPK6/MAPK4 signaling
R-MMU-5689603 UCH proteinases
R-MMU-5689880 Ub-specific processing proteases
R-MMU-6798695 Neutrophil degranulation
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-69229 Ubiquitin-dependent degradation of Cyclin D1
R-MMU-69481 G2/M Checkpoints
R-MMU-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-MMU-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8948751 Regulation of PTEN stability and activity
R-MMU-9020702 Interleukin-1 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 3
Alternative name(s):
26S proteasome regulatory subunit RPN3
26S proteasome regulatory subunit S3
Proteasome subunit p58
Transplantation antigen P91A
Tum-P91A antigen
Gene namesi
Name:Psmd3
Synonyms:P91a, Tstap91a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98858 Psmd3

Subcellular locationi

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738171 – 53026S proteasome non-ATPase regulatory subunit 3Add BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki36Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki36Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei414PhosphoserineBy similarity1
Modified residuei426PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP14685
MaxQBiP14685
PaxDbiP14685
PeptideAtlasiP14685
PRIDEiP14685

PTM databases

iPTMnetiP14685
PhosphoSitePlusiP14685
SwissPalmiP14685

Expressioni

Gene expression databases

BgeeiENSMUSG00000017221
ExpressionAtlasiP14685 baseline and differential
GenevisibleiP14685 MM

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD3, a base containing 6 ATPases and few additional components. Interacts with UBQLN1 (via ubiquitin-like domain).By similarity1 Publication

Protein-protein interaction databases

BioGridi204358, 6 interactors
IntActiP14685, 6 interactors
STRINGi10090.ENSMUSP00000017365

Structurei

3D structure databases

ProteinModelPortaliP14685
SMRiP14685
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini282 – 461PCIPROSITE-ProRule annotationAdd BLAST180

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 30Poly-Pro6

Sequence similaritiesi

Belongs to the proteasome subunit S3 family.Curated

Phylogenomic databases

eggNOGiKOG2581 Eukaryota
ENOG410XS40 LUCA
GeneTreeiENSGT00490000043406
HOGENOMiHOG000193909
HOVERGENiHBG000703
InParanoidiP14685
KOiK03033
OMAiAMRYPPK
OrthoDBiEOG091G05QN
TreeFamiTF106110

Family and domain databases

InterProiView protein in InterPro
IPR013586 26S_Psome_reg_C
IPR000717 PCI_dom
IPR035267 PSMD3
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10758:SF2 PTHR10758:SF2, 1 hit
PfamiView protein in Pfam
PF01399 PCI, 1 hit
PF08375 Rpn3_C, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

P14685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQEGSARRR GADKAKPPPG GEQEPPPPAP QDVEMKEEAA AGSGSTGEGD
60 70 80 90 100
GKAAATEHSQ RELDTVTLED IKEHVRQLEK AVSGKEPRFV LRALRMLPST
110 120 130 140 150
SRRLNHYVLY KAVHGFFTSN NATRDFLLPF LEEPMDTEAD LQFRPRTGKA
160 170 180 190 200
ASAPLLPEVE AYLQLLMVIF LMNSKRYKEA QKISDDLMQK ISTQNRRALD
210 220 230 240 250
LVAAKCYYYH ARVYEFLDKL DVVRSFLHAR LRTATLRHDA DGQATLLNLL
260 270 280 290 300
LRNYLHYSLY DQAEKLVSKS VFPEQANNNE WARYLYYTGR IKAIQLEYSE
310 320 330 340 350
ARRTMTNALR KAPQHTAVGF KQTVHKLLIV VELLLGEIPD RLQFRQPSLK
360 370 380 390 400
RSLMPYFLLT QAVRTGNLAK FNQVLDQFGE KFQTDGTYTL IIRLRHNVIK
410 420 430 440 450
TGVRMISLSY SRISLADIAQ KLQLDSPEDA EFIVAKAIRD GVIEASINHE
460 470 480 490 500
KGYVQSKEMI DIYSTREPQL AFHQRISFCL DIHNMSVKAM RFPPKSYNKD
510 520 530
LESAEERRER EQQDLEFAKE MAEDDDDSFP
Length:530
Mass (Da):60,718
Last modified:July 27, 2011 - v3
Checksum:iCAB9C9AA3A0EEC8D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 13AD → H in AAA37241 (PubMed:2568889).Curated2
Sequence conflicti196R → H in AAA37241 (PubMed:2568889).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25149 Genomic DNA Translation: AAA37241.1
AK145488 mRNA Translation: BAE26466.1
AK164593 mRNA Translation: BAE37842.1
AK165520 mRNA Translation: BAE38235.1
AK166683 mRNA Translation: BAE38942.1
AK166714 mRNA Translation: BAE38965.1
AK167285 mRNA Translation: BAE39391.1
AK169690 mRNA Translation: BAE41307.1
AL590963 Genomic DNA Translation: CAM46197.1
CH466556 Genomic DNA Translation: EDL16159.1
BC003197 mRNA Translation: AAH03197.1
CCDSiCCDS25359.1
PIRiI49504
RefSeqiNP_033465.1, NM_009439.1
UniGeneiMm.12194

Genome annotation databases

EnsembliENSMUST00000017365; ENSMUSP00000017365; ENSMUSG00000017221
GeneIDi22123
KEGGimmu:22123
UCSCiuc007lgw.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPSMD3_MOUSE
AccessioniPrimary (citable) accession number: P14685
Secondary accession number(s): Q3TP95, Q99LL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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