UniProtKB - P14681 (KSS1_YEAST)
Mitogen-activated protein kinase KSS1
KSS1
Functioni
Together with closely related FUS3, KSS1 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated KSS1 activates both pathways, whereas activated FUS3 activates the mating but suppresses the filamentation pathway. KSS1 activity is down-regulated by FUS3 during pheromone induction to prevent inappropriate activation of the filamentation pathway. During induction of filamentation, KSS1 activates the transcription factor STE12 resulting in its binding to and activation of filamentation specific genes. Non-activated KSS1 has a kinase-independent repressive effect on STE12 transcriptional activity, that is mediated by direct binding to STE12 and depends on the presence of DIG1 and DIG2, and that is required for the suppression of filamentation under normal growth conditions. SSN3/SRB10 contributes further to the suppression of filamentation under these conditions by reducing STE12 stability independent of KSS1. FUS3 can partially compensate for the lack of KSS1 but filamentation becomes constitutively induced at a low level in the absence of any signal. KSS1 phosphorylates STE7, STE5, FAR1, DIG1, DIG2, STE12, and SST2.
8 PublicationsMiscellaneous
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 42 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 143 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 19 – 27 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase activity Source: SGD
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- intracellular signal transduction Source: GO_Central
- invasive growth in response to glucose limitation Source: SGD
- negative regulation of DNA-binding transcription factor activity Source: SGD
- pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
- positive regulation of division septum assembly Source: SGD
- protein phosphorylation Source: SGD
- signal transduction involved in filamentous growth Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 984 |
Reactomei | R-SCE-110056, MAPK3 (ERK1) activation R-SCE-112409, RAF-independent MAPK1/3 activation R-SCE-170968, Frs2-mediated activation R-SCE-198753, ERK/MAPK targets R-SCE-202670, ERKs are inactivated R-SCE-3371453, Regulation of HSF1-mediated heat shock response R-SCE-375165, NCAM signaling for neurite out-growth R-SCE-445144, Signal transduction by L1 R-SCE-450341, Activation of the AP-1 family of transcription factors R-SCE-5673001, RAF/MAP kinase cascade R-SCE-5674135, MAP2K and MAPK activation R-SCE-5674499, Negative feedback regulation of MAPK pathway R-SCE-5675221, Negative regulation of MAPK pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase KSS1 (EC:2.7.11.24)Short name: MAP kinase KSS1 Alternative name(s): Kinase suppressor of SST2 |
Gene namesi | Name:KSS1 Ordered Locus Names:YGR040W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003272, KSS1 |
VEuPathDBi | FungiDB:YGR040W |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Other locations
- cellular bud neck Source: SGD
- cytoplasm Source: GO_Central
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Nucleus, PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186333 | 1 – 368 | Mitogen-activated protein kinase KSS1Add BLAST | 368 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 183 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 185 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P14681 |
PaxDbi | P14681 |
PRIDEi | P14681 |
PTM databases
iPTMneti | P14681 |
Interactioni
Subunit structurei
In the nucleus, KSS1 forms a complex with DIG1, DIG2 and STE12; in contrast to FUS3 the interaction of KSS1 with STE12 does not depend on DIG1 and DIG2. Phosphorylated KSS1 shows reduced interaction with STE12. During pheromone activation and phosphorylation, KSS1 forms a membrane-associated complex with the scaffold protein STE5, the MAPKK STE7, the MAPKKK STE11, and the G-protein beta subunit GBB/STE4; interacting directly with POF1, STE7 and STE5 proteins.
Binary interactionsi
P14681
Protein-protein interaction databases
BioGRIDi | 33287, 218 interactors |
DIPi | DIP-60N |
ELMi | P14681 |
IntActi | P14681, 78 interactors |
MINTi | P14681 |
STRINGi | 4932.YGR040W |
Miscellaneous databases
RNActi | P14681, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 313 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 301 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 183 – 185 | TXY | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P14681 |
OMAi | EIMTFRP |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MARTITFDIP SQYKLVDLIG EGAYGTVCSA IHKPSGIKVA IKKIQPFSKK
60 70 80 90 100
LFVTRTIREI KLLRYFHEHE NIISILDKVR PVSIDKLNAV YLVEELMETD
110 120 130 140 150
LQKVINNQNS GFSTLSDDHV QYFTYQILRA LKSIHSAQVI HRDIKPSNLL
160 170 180 190 200
LNSNCDLKVC DFGLARCLAS SSDSRETLVG FMTEYVATRW YRAPEIMLTF
210 220 230 240 250
QEYTTAMDIW SCGCILAEMV SGKPLFPGRD YHHQLWLILE VLGTPSFEDF
260 270 280 290 300
NQIKSKRAKE YIANLPMRPP LPWETVWSKT DLNPDMIDLL DKMLQFNPDK
310 320 330 340 350
RISAAEALRH PYLAMYHDPS DEPEYPPLNL DDEFWKLDNK IMRPEEEEEV
360
PIEMLKDMLY DELMKTME
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26398 Genomic DNA Translation: AAA34882.1 DQ115391 Genomic DNA Translation: AAZ22456.1 Z72825 Genomic DNA Translation: CAA97038.1 AY557773 Genomic DNA Translation: AAS56099.1 BK006941 Genomic DNA Translation: DAA08139.1 |
PIRi | A33297 |
RefSeqi | NP_011554.3, NM_001181169.3 |
Genome annotation databases
EnsemblFungii | YGR040W_mRNA; YGR040W; YGR040W |
GeneIDi | 852931 |
KEGGi | sce:YGR040W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26398 Genomic DNA Translation: AAA34882.1 DQ115391 Genomic DNA Translation: AAZ22456.1 Z72825 Genomic DNA Translation: CAA97038.1 AY557773 Genomic DNA Translation: AAS56099.1 BK006941 Genomic DNA Translation: DAA08139.1 |
PIRi | A33297 |
RefSeqi | NP_011554.3, NM_001181169.3 |
3D structure databases
AlphaFoldDBi | P14681 |
SMRi | P14681 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33287, 218 interactors |
DIPi | DIP-60N |
ELMi | P14681 |
IntActi | P14681, 78 interactors |
MINTi | P14681 |
STRINGi | 4932.YGR040W |
PTM databases
iPTMneti | P14681 |
Proteomic databases
MaxQBi | P14681 |
PaxDbi | P14681 |
PRIDEi | P14681 |
Genome annotation databases
EnsemblFungii | YGR040W_mRNA; YGR040W; YGR040W |
GeneIDi | 852931 |
KEGGi | sce:YGR040W |
Organism-specific databases
SGDi | S000003272, KSS1 |
VEuPathDBi | FungiDB:YGR040W |
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P14681 |
OMAi | EIMTFRP |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 984 |
Reactomei | R-SCE-110056, MAPK3 (ERK1) activation R-SCE-112409, RAF-independent MAPK1/3 activation R-SCE-170968, Frs2-mediated activation R-SCE-198753, ERK/MAPK targets R-SCE-202670, ERKs are inactivated R-SCE-3371453, Regulation of HSF1-mediated heat shock response R-SCE-375165, NCAM signaling for neurite out-growth R-SCE-445144, Signal transduction by L1 R-SCE-450341, Activation of the AP-1 family of transcription factors R-SCE-5673001, RAF/MAP kinase cascade R-SCE-5674135, MAP2K and MAPK activation R-SCE-5674499, Negative feedback regulation of MAPK pathway R-SCE-5675221, Negative regulation of MAPK pathway |
Miscellaneous databases
PROi | PR:P14681 |
RNActi | P14681, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KSS1_YEAST | |
Accessioni | P14681Primary (citable) accession number: P14681 Secondary accession number(s): D6VUH8, Q45U43 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | May 25, 2022 | |
This is version 222 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families