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UniProtKB - P14650 (PERT_RAT)
Protein
Thyroid peroxidase
Gene
Tpo
Organism
Rattus norvegicus (Rat)
Status
Functioni
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.
By similarityCatalytic activityi
- EC:1.11.1.8By similarity
- [thyroglobulin]-L-tyrosine + H+ + H2O2 + iodide = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2OBy similarityEC:1.11.1.8By similarity
- [thyroglobulin]-3-iodo-L-tyrosine + H+ + H2O2 + iodide = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2OBy similarityEC:1.11.1.8By similarity
- 2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-dehydroalanine + [thyroglobulin]-L-thyroxine + 2 H2OBy similarityEC:1.11.1.8By similarity
- [thyroglobulin]-3,5-diiodo-L-tyrosine + [thyroglobulin]-3-iodo-L-tyrosine + H2O2 = [thyroglobulin]-3,3',5-triiodo-L-thyronine + [thyroglobulin]-dehydroalanine + 2 H2OBy similarityEC:1.11.1.8By similarity
Cofactori
Protein has several cofactor binding sites:- Ca2+PROSITE-ProRule annotationNote: Binds 1 Ca2+ ion per heterodimer.PROSITE-ProRule annotation
- heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per heterodimer.PROSITE-ProRule annotation
: thyroid hormone biosynthesis Pathwayi
This protein is involved in the pathway thyroid hormone biosynthesis, which is part of Hormone biosynthesis.View all proteins of this organism that are known to be involved in the pathway thyroid hormone biosynthesis and in Hormone biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 232 | Heme; covalent, via 2 linksBy similarity | 1 | |
Active sitei | 233 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 234 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 313 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 315 | Calcium; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 317 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 319 | CalciumPROSITE-ProRule annotation | 1 | |
Sitei | 384 | Transition state stabilizerPROSITE-ProRule annotation | 1 | |
Binding sitei | 387 | Heme; covalent, via 2 linksBy similarity | 1 | |
Metal bindingi | 482 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- heme binding Source: InterPro
- iodide peroxidase activity Source: RGD
- peroxidase activity Source: GO_Central
GO - Biological processi
- cellular response to nitric oxide Source: RGD
- embryonic hemopoiesis Source: RGD
- hormone biosynthetic process Source: UniProtKB-KW
- hydrogen peroxide catabolic process Source: UniProtKB-KW
- response to lipid Source: RGD
- response to oxidative stress Source: InterPro
- thyroid hormone generation Source: RGD
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Hydrogen peroxide, Thyroid hormones biosynthesis |
Ligand | Calcium, Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-209968, Thyroxine biosynthesis |
UniPathwayi | UPA00194 |
Protein family/group databases
PeroxiBasei | 3973, RnoTPO |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Tpo |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3900, Tpo |
Subcellular locationi
Other locations
- Membrane By similarity; Single-pass type I membrane protein By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- cell surface Source: RGD
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 32 – 834 | ExtracellularSequence analysisAdd BLAST | 803 | |
Transmembranei | 835 – 859 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 860 – 914 | CytoplasmicSequence analysisAdd BLAST | 55 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Sequence analysisAdd BLAST | 31 | |
ChainiPRO_0000023665 | 32 – 914 | Thyroid peroxidaseAdd BLAST | 883 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 123 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 136 ↔ 152 | By similarity | ||
Disulfide bondi | 253 ↔ 263 | By similarity | ||
Disulfide bondi | 257 ↔ 278 | By similarity | ||
Glycosylationi | 271 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 299 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 334 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 586 ↔ 643 | By similarity | ||
Glycosylationi | 603 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 684 ↔ 709 | By similarity | ||
Disulfide bondi | 730 ↔ 770 | By similarity | ||
Disulfide bondi | 756 ↔ 782 | By similarity | ||
Disulfide bondi | 788 ↔ 802 | By similarity | ||
Disulfide bondi | 796 ↔ 811 | By similarity | ||
Disulfide bondi | 813 ↔ 826 | By similarity |
Post-translational modificationi
Heme is covalently bound through a H2O2-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface (By similarity).By similarity
Cleaved in its N-terminal part.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | P14650 |
PaxDbi | P14650 |
PTM databases
GlyGeni | P14650, 5 sites |
Interactioni
Subunit structurei
Interacts with DUOX1, DUOX2 and CYBA.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000006526 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 728 – 783 | SushiPROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 784 – 827 | EGF-like; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 44 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 882 – 907 | DisorderedSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Signal, Sushi, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2408, Eukaryota |
InParanoidi | P14650 |
PhylomeDBi | P14650 |
Family and domain databases
CDDi | cd00033, CCP, 1 hit |
Gene3Di | 1.10.640.10, 1 hit |
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom IPR029589, TPO |
PANTHERi | PTHR11475:SF60, PTHR11475:SF60, 1 hit |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF07645, EGF_CA, 1 hit PF00084, Sushi, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00032, CCP, 1 hit SM00181, EGF, 1 hit SM00179, EGF_CA, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit SSF57535, SSF57535, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00435, PEROXIDASE_1, 1 hit PS50292, PEROXIDASE_3, 1 hit PS50923, SUSHI, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P14650-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTLGAMAVM LVVMGTAIFL PFLLRSRDIL GGKTMTSHVI SVVETSQLLV
60 70 80 90 100
DNAVYNTMKR NLKKRGVLSP AQLLSFSKLP ESTSGAISRA AEIMETSIQV
110 120 130 140 150
MKREQSQFST DALSADILAT IANLSGCLPF MLPPRCPDTC LANKYRPITG
160 170 180 190 200
VCNNRDHPRW GASNTALARW LPPVYEDGFS QPRGWNPNFL YHGFPLPPVR
210 220 230 240 250
EVTRHLIQVS NEAVTEDDQY SDFLPVWGQY IDHDIALTPQ STSTAAFWGG
260 270 280 290 300
VDCQLTCENQ NPCFPIQLPS NSSRTTACLP FYRSSAACGT GDQGALFGNL
310 320 330 340 350
SAANPRQQMN GLTSFLDAST VYGSSPGVEK QLRNWSSSAG LLRVNTLHLD
360 370 380 390 400
SGRAYLPFAS AACAPEPGAP HANRTPCFLA GDGRASEVPA LAAVHTLWLR
410 420 430 440 450
EHNRLATAFK AINTHWSANT AYQEARKVVG ALHQIITMRD YIPKILGPDA
460 470 480 490 500
FRQYVGPYEG YNPTVNPTVS NVFSTAAFRF GHATVHPLVR RLNTDFQDHT
510 520 530 540 550
ELPRLQLHDV FFRPWRLIQE GGLDPIVRGL LARPAKLQVQ EQLMNEELTE
560 570 580 590 600
RLFVLSNVGT LDLASLNLQR GRDHGLPGYN EWREFCGLSR LDTGAELNKA
610 620 630 640 650
IANRSMVNKI MELYKHADNI DVWLGGLAEK FLPGARTGPL FACIIGKQMK
660 670 680 690 700
ALRDGDRFWW ENSHVFTDAQ RQELEKHSLP RVICDNTGLT RVPVDAFRIG
710 720 730 740 750
KFPQDFESCE EIPSMDLRLW RETFPQDDKC VFPEKVDNGN FVHCEESGKL
760 770 780 790 800
VLVYSCFHGY KLQGQEQVTC TQNGWDSEPP VCKDVNECAD LTHPPCHSSA
810 820 830 840 850
KCKNTKGSFQ CVCTDPYMLG EDEKTCIDSG RLPRASWVSI ALGALLIGGL
860 870 880 890 900
ASLSWTVICR WTHADKKSTL LITERVTMES GFRKSQESGI SPQKAEVQDA
910
EQEPAYGSRV LLCE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1LN48 | F1LN48_RAT | Thyroid peroxidase | Tpo | 914 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 194 – 195 | FP → LG in AAA42265 (PubMed:2691880).Curated | 2 | |
Sequence conflicti | 198 | P → S in AAA42265 (PubMed:2691880).Curated | 1 | |
Sequence conflicti | 228 | G → A in AAA42265 (PubMed:2691880).Curated | 1 | |
Sequence conflicti | 592 – 594 | DTG → ETP in AAA42265 (PubMed:2691880).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17396 mRNA Translation: CAA35257.1 M31655 mRNA Translation: AAA42265.1 |
PIRi | S07047 |
Genome annotation databases
UCSCi | RGD:3900, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17396 mRNA Translation: CAA35257.1 M31655 mRNA Translation: AAA42265.1 |
PIRi | S07047 |
3D structure databases
SMRi | P14650 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000006526 |
Protein family/group databases
PeroxiBasei | 3973, RnoTPO |
PTM databases
GlyGeni | P14650, 5 sites |
Proteomic databases
jPOSTi | P14650 |
PaxDbi | P14650 |
Genome annotation databases
UCSCi | RGD:3900, rat |
Organism-specific databases
RGDi | 3900, Tpo |
Phylogenomic databases
eggNOGi | KOG2408, Eukaryota |
InParanoidi | P14650 |
PhylomeDBi | P14650 |
Enzyme and pathway databases
UniPathwayi | UPA00194 |
Reactomei | R-RNO-209968, Thyroxine biosynthesis |
Miscellaneous databases
PROi | PR:P14650 |
Family and domain databases
CDDi | cd00033, CCP, 1 hit |
Gene3Di | 1.10.640.10, 1 hit |
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom IPR029589, TPO |
PANTHERi | PTHR11475:SF60, PTHR11475:SF60, 1 hit |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF07645, EGF_CA, 1 hit PF00084, Sushi, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00032, CCP, 1 hit SM00181, EGF, 1 hit SM00179, EGF_CA, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit SSF57535, SSF57535, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00435, PEROXIDASE_1, 1 hit PS50292, PEROXIDASE_3, 1 hit PS50923, SUSHI, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PERT_RAT | |
Accessioni | P14650Primary (citable) accession number: P14650 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | February 23, 2022 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families