UniProtKB - P14635 (CCNB1_HUMAN)
G2/mitotic-specific cyclin-B1
CCNB1
Functioni
GO - Molecular functioni
- cyclin-dependent protein serine/threonine kinase activator activity Source: CAFA
- cyclin-dependent protein serine/threonine kinase regulator activity Source: GO_Central
- patched binding Source: BHF-UCL
- protein kinase binding Source: UniProtKB
- ubiquitin-like protein ligase binding Source: UniProtKB
GO - Biological processi
- anaphase-promoting complex-dependent catabolic process Source: Reactome
- cell division Source: UniProtKB-KW
- cellular response to fatty acid Source: Ensembl
- cellular response to hypoxia Source: Ensembl
- cellular response to iron(III) ion Source: Ensembl
- cellular response to organic cyclic compound Source: Ensembl
- digestive tract development Source: Ensembl
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
- G2/M transition of mitotic cell cycle Source: Reactome
- histone H3-S10 phosphorylation involved in chromosome condensation Source: Ensembl
- in utero embryonic development Source: Ensembl
- mitotic cell cycle phase transition Source: GO_Central
- mitotic metaphase plate congression Source: BHF-UCL
- mitotic nuclear envelope disassembly Source: Reactome
- mitotic spindle organization Source: BHF-UCL
- negative regulation of gene expression Source: Ensembl
- negative regulation of protein phosphorylation Source: Ensembl
- oocyte maturation Source: Ensembl
- positive regulation of attachment of spindle microtubules to kinetochore Source: BHF-UCL
- positive regulation of cardiac muscle cell proliferation Source: Ensembl
- positive regulation of fibroblast proliferation Source: Ensembl
- positive regulation of G2/M transition of mitotic cell cycle Source: CAFA
- positive regulation of histone phosphorylation Source: Ensembl
- positive regulation of mitochondrial ATP synthesis coupled electron transport Source: CAFA
- positive regulation of mitotic cell cycle Source: BHF-UCL
- positive regulation of mRNA 3'-end processing Source: Ensembl
- protein-containing complex assembly Source: Ensembl
- regulation of cell cycle Source: Reactome
- regulation of chromosome condensation Source: Ensembl
- regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
- regulation of mitotic cell cycle phase transition Source: Reactome
- regulation of mitotic cell cycle spindle assembly checkpoint Source: BHF-UCL
- response to DDT Source: Ensembl
- response to drug Source: Ensembl
- response to mechanical stimulus Source: Ensembl
- spermatogenesis Source: Ensembl
- tissue regeneration Source: Ensembl
- transcription initiation from RNA polymerase II promoter Source: Reactome
- ventricular cardiac muscle cell development Source: Ensembl
Keywordsi
Molecular function | Cyclin |
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | P14635 |
Reactomei | R-HSA-113507, E2F-enabled inhibition of pre-replication complex formation R-HSA-156711, Polo-like kinase mediated events R-HSA-162658, Golgi Cisternae Pericentriolar Stack Reorganization R-HSA-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-HSA-176408, Regulation of APC/C activators between G1/S and early anaphase R-HSA-176412, Phosphorylation of the APC/C R-HSA-176417, Phosphorylation of Emi1 R-HSA-2299718, Condensation of Prophase Chromosomes R-HSA-2465910, MASTL Facilitates Mitotic Progression R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-2514853, Condensation of Prometaphase Chromosomes R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-HSA-2995383, Initiation of Nuclear Envelope (NE) Reformation R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-4419969, Depolymerisation of the Nuclear Lamina R-HSA-6804114, TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-HSA-68875, Mitotic Prophase R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition R-HSA-69478, G2/M DNA replication checkpoint R-HSA-75035, Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-HSA-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-8878166, Transcriptional regulation by RUNX2 |
SignaLinki | P14635 |
SIGNORi | P14635 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: G2/mitotic-specific cyclin-B1 |
Gene namesi | Name:CCNB1 Synonyms:CCNB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000134057.14 |
HGNCi | HGNC:1579, CCNB1 |
MIMi | 123836, gene |
neXtProti | NX_P14635 |
Subcellular locationi
Cytoskeleton
Nucleus
Other locations
Cytoskeleton
- centrosome Source: UniProtKB
- spindle pole Source: BHF-UCL
Cytosol
- cytosol Source: HPA
Mitochondrion
- mitochondrial matrix Source: Ensembl
Nucleus
- condensed nuclear chromosome outer kinetochore Source: BHF-UCL
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- cyclin B1-CDK1 complex Source: CAFA
- cyclin-dependent protein kinase holoenzyme complex Source: GO_Central
- cytoplasm Source: UniProtKB
- membrane Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 133 | S → A: Strongly impairs phosphorylation by PLK1. 2 Publications | 1 | |
Mutagenesisi | 147 | S → A: Does not affect phosphorylation by PLK1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 891 |
OpenTargetsi | ENSG00000134057 |
PharmGKBi | PA95 |
Miscellaneous databases
Pharosi | P14635, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2412 |
DrugCentrali | P14635 |
Polymorphism and mutation databases
BioMutai | CCNB1 |
DMDMi | 116176 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080350 | 1 – 433 | G2/mitotic-specific cyclin-B1Add BLAST | 433 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 73 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 126 | Phosphoserine; by CDK12 Publications | 1 | |
Modified residuei | 128 | Phosphoserine1 Publication | 1 | |
Modified residuei | 133 | Phosphoserine; by PLK12 Publications | 1 | |
Modified residuei | 147 | Phosphoserine2 Publications | 1 | |
Modified residuei | 321 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | CPTAC-1236 |
EPDi | P14635 |
jPOSTi | P14635 |
MassIVEi | P14635 |
MaxQBi | P14635 |
PaxDbi | P14635 |
PeptideAtlasi | P14635 |
PRIDEi | P14635 |
ProteomicsDBi | 53068 [P14635-1] 65222 |
PTM databases
iPTMneti | P14635 |
MetOSitei | P14635 |
PhosphoSitePlusi | P14635 |
SwissPalmi | P14635 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSG00000134057, Expressed in secondary oocyte and 182 other tissues |
ExpressionAtlasi | P14635, baseline and differential |
Genevisiblei | P14635, HS |
Organism-specific databases
HPAi | ENSG00000134057, Tissue enhanced (lymphoid) |
Interactioni
Subunit structurei
Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10.
Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
Interacts with CCNF; interaction is required for nuclear localization.
Interacts with CDK5RAP3 (PubMed:15790566).
Interacts with RFPL4A and UBE2A (By similarity).
Interacts with INCA1 (PubMed:21540187).
By similarity7 PublicationsBinary interactionsi
Hide detailsP14635
GO - Molecular functioni
- patched binding Source: BHF-UCL
- protein kinase binding Source: UniProtKB
- ubiquitin-like protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107332, 167 interactors |
ComplexPortali | CPX-2007, Cyclin B1-CDK1 complex |
CORUMi | P14635 |
DIPi | DIP-59N |
ELMi | P14635 |
IntActi | P14635, 75 interactors |
MINTi | P14635 |
STRINGi | 9606.ENSP00000256442 |
Chemistry databases
BindingDBi | P14635 |
Miscellaneous databases
RNActi | P14635, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P14635 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14635 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 169 – 177 | Interaction with CDK2 | 9 | |
Regioni | 258 – 261 | Interaction with CDK2 | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 51 – 85 | Lys-richAdd BLAST | 35 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0653, Eukaryota |
GeneTreei | ENSGT00940000154586 |
HOGENOMi | CLU_020695_2_1_1 |
InParanoidi | P14635 |
OMAi | CAEENCQ |
OrthoDBi | 993640at2759 |
PhylomeDBi | P14635 |
TreeFami | TF101001 |
Family and domain databases
CDDi | cd00043, CYCLIN, 2 hits |
DisProti | DP00223 |
IDEALi | IID00141 |
InterProi | View protein in InterPro IPR039361, Cyclin IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR004367, Cyclin_C-dom IPR006671, Cyclin_N |
PANTHERi | PTHR10177, PTHR10177, 1 hit |
Pfami | View protein in Pfam PF02984, Cyclin_C, 1 hit PF00134, Cyclin_N, 1 hit |
PIRSFi | PIRSF001771, Cyclin_A_B_D_E, 1 hit |
SMARTi | View protein in SMART SM00385, CYCLIN, 2 hits SM01332, Cyclin_C, 1 hit |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00292, CYCLINS, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALRVTRNSK INAENKAKIN MAGAKRVPTA PAATSKPGLR PRTALGDIGN
60 70 80 90 100
KVSEQLQAKM PMKKEAKPSA TGKVIDKKLP KPLEKVPMLV PVPVSEPVPE
110 120 130 140 150
PEPEPEPEPV KEEKLSPEPI LVDTASPSPM ETSGCAPAEE DLCQAFSDVI
160 170 180 190 200
LAVNDVDAED GADPNLCSEY VKDIYAYLRQ LEEEQAVRPK YLLGREVTGN
210 220 230 240 250
MRAILIDWLV QVQMKFRLLQ ETMYMTVSII DRFMQNNCVP KKMLQLVGVT
260 270 280 290 300
AMFIASKYEE MYPPEIGDFA FVTDNTYTKH QIRQMEMKIL RALNFGLGRP
310 320 330 340 350
LPLHFLRRAS KIGEVDVEQH TLAKYLMELT MLDYDMVHFP PSQIAAGAFC
360 370 380 390 400
LALKILDNGE WTPTLQHYLS YTEESLLPVM QHLAKNVVMV NQGLTKHMTV
410 420 430
KNKYATSKHA KISTLPQLNS ALVQDLAKAV AKV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RHI0 | D6RHI0_HUMAN | G2/mitotic-specific cyclin-B1 | CCNB1 | 244 | Annotation score: | ||
E9PC90 | E9PC90_HUMAN | G2/mitotic-specific cyclin-B1 | CCNB1 | 401 | Annotation score: | ||
H0YA62 | H0YA62_HUMAN | G2/mitotic-specific cyclin-B1 | CCNB1 | 185 | Annotation score: | ||
H0Y9U8 | H0Y9U8_HUMAN | G2/mitotic-specific cyclin-B1 | CCNB1 | 61 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 108 | E → G in BAF82120 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 153 | V → A in BAF82120 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053892 | 362 – 398 | Missing in isoform 2. 1 PublicationAdd BLAST | 37 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25753 mRNA No translation available. AY338491 Genomic DNA Translation: AAP88038.1 AK289431 mRNA Translation: BAF82120.1 BT020128 mRNA Translation: AAV38930.1 AC010273 Genomic DNA No translation available. AC022107 Genomic DNA No translation available. BC006510 mRNA Translation: AAH06510.1 |
CCDSi | CCDS3997.1 [P14635-1] |
PIRi | A32992 |
RefSeqi | NP_114172.1, NM_031966.3 [P14635-1] |
Genome annotation databases
Ensembli | ENST00000256442; ENSP00000256442; ENSG00000134057 [P14635-1] ENST00000505500; ENSP00000424588; ENSG00000134057 [P14635-2] |
GeneIDi | 891 |
KEGGi | hsa:891 |
UCSCi | uc003jvm.4, human [P14635-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25753 mRNA No translation available. AY338491 Genomic DNA Translation: AAP88038.1 AK289431 mRNA Translation: BAF82120.1 BT020128 mRNA Translation: AAV38930.1 AC010273 Genomic DNA No translation available. AC022107 Genomic DNA No translation available. BC006510 mRNA Translation: AAH06510.1 |
CCDSi | CCDS3997.1 [P14635-1] |
PIRi | A32992 |
RefSeqi | NP_114172.1, NM_031966.3 [P14635-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2B9R | X-ray | 2.90 | A/B | 165-433 | [»] | |
2JGZ | X-ray | 2.90 | B | 167-426 | [»] | |
4Y72 | X-ray | 2.30 | B | 165-433 | [»] | |
4YC3 | X-ray | 2.70 | B | 165-433 | [»] | |
5HQ0 | X-ray | 2.30 | B | 165-433 | [»] | |
5LQF | X-ray | 2.06 | B/E | 165-433 | [»] | |
6GU2 | X-ray | 2.00 | B | 165-433 | [»] | |
6GU3 | X-ray | 2.65 | B | 165-433 | [»] | |
6GU4 | X-ray | 2.73 | B | 165-433 | [»] | |
SMRi | P14635 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107332, 167 interactors |
ComplexPortali | CPX-2007, Cyclin B1-CDK1 complex |
CORUMi | P14635 |
DIPi | DIP-59N |
ELMi | P14635 |
IntActi | P14635, 75 interactors |
MINTi | P14635 |
STRINGi | 9606.ENSP00000256442 |
Chemistry databases
BindingDBi | P14635 |
ChEMBLi | CHEMBL2412 |
DrugCentrali | P14635 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | P14635 |
MetOSitei | P14635 |
PhosphoSitePlusi | P14635 |
SwissPalmi | P14635 |
Polymorphism and mutation databases
BioMutai | CCNB1 |
DMDMi | 116176 |
Proteomic databases
CPTACi | CPTAC-1236 |
EPDi | P14635 |
jPOSTi | P14635 |
MassIVEi | P14635 |
MaxQBi | P14635 |
PaxDbi | P14635 |
PeptideAtlasi | P14635 |
PRIDEi | P14635 |
ProteomicsDBi | 53068 [P14635-1] 65222 |
Protocols and materials databases
Antibodypediai | 3537, 1825 antibodies |
DNASUi | 891 |
Genome annotation databases
Ensembli | ENST00000256442; ENSP00000256442; ENSG00000134057 [P14635-1] ENST00000505500; ENSP00000424588; ENSG00000134057 [P14635-2] |
GeneIDi | 891 |
KEGGi | hsa:891 |
UCSCi | uc003jvm.4, human [P14635-1] |
Organism-specific databases
CTDi | 891 |
DisGeNETi | 891 |
EuPathDBi | HostDB:ENSG00000134057.14 |
GeneCardsi | CCNB1 |
HGNCi | HGNC:1579, CCNB1 |
HPAi | ENSG00000134057, Tissue enhanced (lymphoid) |
MIMi | 123836, gene |
neXtProti | NX_P14635 |
OpenTargetsi | ENSG00000134057 |
PharmGKBi | PA95 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0653, Eukaryota |
GeneTreei | ENSGT00940000154586 |
HOGENOMi | CLU_020695_2_1_1 |
InParanoidi | P14635 |
OMAi | CAEENCQ |
OrthoDBi | 993640at2759 |
PhylomeDBi | P14635 |
TreeFami | TF101001 |
Enzyme and pathway databases
PathwayCommonsi | P14635 |
Reactomei | R-HSA-113507, E2F-enabled inhibition of pre-replication complex formation R-HSA-156711, Polo-like kinase mediated events R-HSA-162658, Golgi Cisternae Pericentriolar Stack Reorganization R-HSA-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-HSA-176408, Regulation of APC/C activators between G1/S and early anaphase R-HSA-176412, Phosphorylation of the APC/C R-HSA-176417, Phosphorylation of Emi1 R-HSA-2299718, Condensation of Prophase Chromosomes R-HSA-2465910, MASTL Facilitates Mitotic Progression R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-2514853, Condensation of Prometaphase Chromosomes R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-HSA-2995383, Initiation of Nuclear Envelope (NE) Reformation R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-4419969, Depolymerisation of the Nuclear Lamina R-HSA-6804114, TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-HSA-68875, Mitotic Prophase R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition R-HSA-69478, G2/M DNA replication checkpoint R-HSA-75035, Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-HSA-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-8878166, Transcriptional regulation by RUNX2 |
SignaLinki | P14635 |
SIGNORi | P14635 |
Miscellaneous databases
BioGRID-ORCSi | 891, 483 hits in 851 CRISPR screens |
ChiTaRSi | CCNB1, human |
EvolutionaryTracei | P14635 |
GeneWikii | Cyclin_B1 |
GenomeRNAii | 891 |
Pharosi | P14635, Tchem |
PROi | PR:P14635 |
RNActi | P14635, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134057, Expressed in secondary oocyte and 182 other tissues |
ExpressionAtlasi | P14635, baseline and differential |
Genevisiblei | P14635, HS |
Family and domain databases
CDDi | cd00043, CYCLIN, 2 hits |
DisProti | DP00223 |
IDEALi | IID00141 |
InterProi | View protein in InterPro IPR039361, Cyclin IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR004367, Cyclin_C-dom IPR006671, Cyclin_N |
PANTHERi | PTHR10177, PTHR10177, 1 hit |
Pfami | View protein in Pfam PF02984, Cyclin_C, 1 hit PF00134, Cyclin_N, 1 hit |
PIRSFi | PIRSF001771, Cyclin_A_B_D_E, 1 hit |
SMARTi | View protein in SMART SM00385, CYCLIN, 2 hits SM01332, Cyclin_C, 1 hit |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00292, CYCLINS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCNB1_HUMAN | |
Accessioni | P14635Primary (citable) accession number: P14635 Secondary accession number(s): A8K066, Q5TZP9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 214 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families