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Protein

G2/mitotic-specific cyclin-B1

Gene

CCNB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCyclin
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-113507 E2F-enabled inhibition of pre-replication complex formation
R-HSA-156711 Polo-like kinase mediated events
R-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-176417 Phosphorylation of Emi1
R-HSA-2299718 Condensation of Prophase Chromosomes
R-HSA-2465910 MASTL Facilitates Mitotic Progression
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2514853 Condensation of Prometaphase Chromosomes
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
R-HSA-4419969 Depolymerisation of the Nuclear Lamina
R-HSA-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-HSA-68875 Mitotic Prophase
R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-HSA-69478 G2/M DNA replication checkpoint
R-HSA-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8878166 Transcriptional regulation by RUNX2
SignaLinkiP14635
SIGNORiP14635

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-B1
Gene namesi
Name:CCNB1
Synonyms:CCNB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000134057.14
HGNCiHGNC:1579 CCNB1
MIMi123836 gene
neXtProtiNX_P14635

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133S → A: Strongly impairs phosphorylation by PLK1. 2 Publications1
Mutagenesisi147S → A: Does not affect phosphorylation by PLK1. 1 Publication1

Organism-specific databases

DisGeNETi891
OpenTargetsiENSG00000134057
PharmGKBiPA95

Chemistry databases

ChEMBLiCHEMBL2412

Polymorphism and mutation databases

BioMutaiCCNB1
DMDMi116176

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000803501 – 433G2/mitotic-specific cyclin-B1Add BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73N6-acetyllysineCombined sources1
Modified residuei126Phosphoserine; by CDK12 Publications1
Modified residuei128Phosphoserine1 Publication1
Modified residuei133Phosphoserine; by PLK12 Publications1
Modified residuei147Phosphoserine2 Publications1
Modified residuei321PhosphothreonineCombined sources1

Post-translational modificationi

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.1 Publication
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP14635
MaxQBiP14635
PaxDbiP14635
PeptideAtlasiP14635
PRIDEiP14635
ProteomicsDBi53068

PTM databases

iPTMnetiP14635
PhosphoSitePlusiP14635

Expressioni

Developmental stagei

Accumulates steadily during G2 and is abruptly destroyed at mitosis.1 Publication

Gene expression databases

BgeeiENSG00000134057 Expressed in 168 organ(s), highest expression level in secondary oocyte
CleanExiHS_CCNB1
ExpressionAtlasiP14635 baseline and differential
GenevisibleiP14635 HS

Organism-specific databases

HPAiCAB000115
CAB003804
HPA030741
HPA061448

Interactioni

Subunit structurei

Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3 (PubMed:15790566). Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1 (PubMed:21540187).By similarity7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107332, 148 interactors
ComplexPortaliCPX-2007 Cyclin B1-CDK1 complex
CORUMiP14635
DIPiDIP-59N
ELMiP14635
IntActiP14635, 61 interactors
MINTiP14635
STRINGi9606.ENSP00000256442

Chemistry databases

BindingDBiP14635

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00223
ProteinModelPortaliP14635
SMRiP14635
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14635

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni169 – 177Interaction with CDK29
Regioni258 – 261Interaction with CDK24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi51 – 85Lys-richAdd BLAST35

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

eggNOGiKOG0653 Eukaryota
COG5024 LUCA
GeneTreeiENSGT00760000118939
HOGENOMiHOG000167672
HOVERGENiHBG061650
InParanoidiP14635
KOiK05868
OMAiHMTVKNK
OrthoDBiEOG091G0E9B
PhylomeDBiP14635
TreeFamiTF101001

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR039361 Cyclin
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR004367 Cyclin_C-dom
IPR006671 Cyclin_N
PANTHERiPTHR10177 PTHR10177, 1 hit
PfamiView protein in Pfam
PF02984 Cyclin_C, 1 hit
PF00134 Cyclin_N, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SM01332 Cyclin_C, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00292 CYCLINS, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P14635-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALRVTRNSK INAENKAKIN MAGAKRVPTA PAATSKPGLR PRTALGDIGN
60 70 80 90 100
KVSEQLQAKM PMKKEAKPSA TGKVIDKKLP KPLEKVPMLV PVPVSEPVPE
110 120 130 140 150
PEPEPEPEPV KEEKLSPEPI LVDTASPSPM ETSGCAPAEE DLCQAFSDVI
160 170 180 190 200
LAVNDVDAED GADPNLCSEY VKDIYAYLRQ LEEEQAVRPK YLLGREVTGN
210 220 230 240 250
MRAILIDWLV QVQMKFRLLQ ETMYMTVSII DRFMQNNCVP KKMLQLVGVT
260 270 280 290 300
AMFIASKYEE MYPPEIGDFA FVTDNTYTKH QIRQMEMKIL RALNFGLGRP
310 320 330 340 350
LPLHFLRRAS KIGEVDVEQH TLAKYLMELT MLDYDMVHFP PSQIAAGAFC
360 370 380 390 400
LALKILDNGE WTPTLQHYLS YTEESLLPVM QHLAKNVVMV NQGLTKHMTV
410 420 430
KNKYATSKHA KISTLPQLNS ALVQDLAKAV AKV
Length:433
Mass (Da):48,337
Last modified:April 1, 1990 - v1
Checksum:iE2C4767AE8A11EC0
GO
Isoform 2 (identifier: P14635-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     362-398: Missing.

Note: No experimental confirmation available.
Show »
Length:396
Mass (Da):44,102
Checksum:i1B7AFCE21614F6D5
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PC90E9PC90_HUMAN
G2/mitotic-specific cyclin-B1
CCNB1
401Annotation score:
D6RHI0D6RHI0_HUMAN
G2/mitotic-specific cyclin-B1
CCNB1
244Annotation score:
H0YA62H0YA62_HUMAN
G2/mitotic-specific cyclin-B1
CCNB1
185Annotation score:
H0Y9U8H0Y9U8_HUMAN
G2/mitotic-specific cyclin-B1
CCNB1
61Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108E → G in BAF82120 (PubMed:14702039).Curated1
Sequence conflicti153V → A in BAF82120 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053892362 – 398Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25753 mRNA No translation available.
AY338491 Genomic DNA Translation: AAP88038.1
AK289431 mRNA Translation: BAF82120.1
BT020128 mRNA Translation: AAV38930.1
AC010273 Genomic DNA No translation available.
AC022107 Genomic DNA No translation available.
BC006510 mRNA Translation: AAH06510.1
CCDSiCCDS3997.1 [P14635-1]
PIRiA32992
RefSeqiNP_114172.1, NM_031966.3 [P14635-1]
UniGeneiHs.23960

Genome annotation databases

EnsembliENST00000256442; ENSP00000256442; ENSG00000134057 [P14635-1]
ENST00000505500; ENSP00000424588; ENSG00000134057 [P14635-2]
GeneIDi891
KEGGihsa:891
UCSCiuc003jvm.4 human [P14635-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25753 mRNA No translation available.
AY338491 Genomic DNA Translation: AAP88038.1
AK289431 mRNA Translation: BAF82120.1
BT020128 mRNA Translation: AAV38930.1
AC010273 Genomic DNA No translation available.
AC022107 Genomic DNA No translation available.
BC006510 mRNA Translation: AAH06510.1
CCDSiCCDS3997.1 [P14635-1]
PIRiA32992
RefSeqiNP_114172.1, NM_031966.3 [P14635-1]
UniGeneiHs.23960

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B9RX-ray2.90A/B165-433[»]
2JGZX-ray2.90B167-426[»]
4Y72X-ray2.30B165-433[»]
4YC3X-ray2.70B165-433[»]
5HQ0X-ray2.30B165-433[»]
5LQFX-ray2.06B/E165-433[»]
DisProtiDP00223
ProteinModelPortaliP14635
SMRiP14635
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107332, 148 interactors
ComplexPortaliCPX-2007 Cyclin B1-CDK1 complex
CORUMiP14635
DIPiDIP-59N
ELMiP14635
IntActiP14635, 61 interactors
MINTiP14635
STRINGi9606.ENSP00000256442

Chemistry databases

BindingDBiP14635
ChEMBLiCHEMBL2412

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiP14635
PhosphoSitePlusiP14635

Polymorphism and mutation databases

BioMutaiCCNB1
DMDMi116176

Proteomic databases

EPDiP14635
MaxQBiP14635
PaxDbiP14635
PeptideAtlasiP14635
PRIDEiP14635
ProteomicsDBi53068

Protocols and materials databases

DNASUi891
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000256442; ENSP00000256442; ENSG00000134057 [P14635-1]
ENST00000505500; ENSP00000424588; ENSG00000134057 [P14635-2]
GeneIDi891
KEGGihsa:891
UCSCiuc003jvm.4 human [P14635-1]

Organism-specific databases

CTDi891
DisGeNETi891
EuPathDBiHostDB:ENSG00000134057.14
GeneCardsiCCNB1
HGNCiHGNC:1579 CCNB1
HPAiCAB000115
CAB003804
HPA030741
HPA061448
MIMi123836 gene
neXtProtiNX_P14635
OpenTargetsiENSG00000134057
PharmGKBiPA95
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0653 Eukaryota
COG5024 LUCA
GeneTreeiENSGT00760000118939
HOGENOMiHOG000167672
HOVERGENiHBG061650
InParanoidiP14635
KOiK05868
OMAiHMTVKNK
OrthoDBiEOG091G0E9B
PhylomeDBiP14635
TreeFamiTF101001

Enzyme and pathway databases

ReactomeiR-HSA-113507 E2F-enabled inhibition of pre-replication complex formation
R-HSA-156711 Polo-like kinase mediated events
R-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-176417 Phosphorylation of Emi1
R-HSA-2299718 Condensation of Prophase Chromosomes
R-HSA-2465910 MASTL Facilitates Mitotic Progression
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2514853 Condensation of Prometaphase Chromosomes
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
R-HSA-4419969 Depolymerisation of the Nuclear Lamina
R-HSA-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-HSA-68875 Mitotic Prophase
R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-HSA-69478 G2/M DNA replication checkpoint
R-HSA-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8878166 Transcriptional regulation by RUNX2
SignaLinkiP14635
SIGNORiP14635

Miscellaneous databases

ChiTaRSiCCNB1 human
EvolutionaryTraceiP14635
GeneWikiiCyclin_B1
GenomeRNAii891
PROiPR:P14635
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134057 Expressed in 168 organ(s), highest expression level in secondary oocyte
CleanExiHS_CCNB1
ExpressionAtlasiP14635 baseline and differential
GenevisibleiP14635 HS

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR039361 Cyclin
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR004367 Cyclin_C-dom
IPR006671 Cyclin_N
PANTHERiPTHR10177 PTHR10177, 1 hit
PfamiView protein in Pfam
PF02984 Cyclin_C, 1 hit
PF00134 Cyclin_N, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SM01332 Cyclin_C, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00292 CYCLINS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCCNB1_HUMAN
AccessioniPrimary (citable) accession number: P14635
Secondary accession number(s): A8K066, Q5TZP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 10, 2018
This is version 195 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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