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Protein

Endoplasmin

Gene

HSP90B1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ATPBy similarity1
Binding sitei149ATPBy similarity1
Binding sitei162ATPBy similarity1
Binding sitei168ATPBy similarity1
Binding sitei199ATP; via amide nitrogenBy similarity1
Binding sitei448ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: UniProtKB
  • low-density lipoprotein particle receptor binding Source: MGI
  • protein phosphatase binding Source: MGI
  • RNA binding Source: UniProtKB
  • unfolded protein binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionChaperone
LigandATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1679131 Trafficking and processing of endosomal TLR
R-HSA-3000480 Scavenging by Class A Receptors
R-HSA-381183 ATF6 (ATF6-alpha) activates chaperone genes
R-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
R-HSA-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin
Alternative name(s):
94 kDa glucose-regulated protein
Short name:
GRP-94
Heat shock protein 90 kDa beta member 1
Tumor rejection antigen 1
gp96 homolog
Gene namesi
Name:HSP90B1
Synonyms:GRP94, TRA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000166598.12
HGNCiHGNC:12028 HSP90B1
MIMi191175 gene
neXtProtiNX_P14625

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

DisGeNETi7184
OpenTargetsiENSG00000166598
PharmGKBiPA36705

Chemistry databases

ChEMBLiCHEMBL1075323
DrugBankiDB02935 1-Methoxy-2-(2-Methoxyethoxy)Ethane
DB02103 2-Chlorodideoxyadenosine
DB03719 N-Ethyl-5'-Carboxamido Adenosine
DB03758 Radicicol
DB00615 Rifabutin
GuidetoPHARMACOLOGYi2904

Polymorphism and mutation databases

DMDMi119360

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Combined sources1 PublicationAdd BLAST21
ChainiPRO_000001359822 – 803EndoplasminAdd BLAST782

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei64PhosphoserineCombined sources1
Glycosylationi107N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi138InterchainBy similarity
Modified residuei172PhosphoserineBy similarity1
Glycosylationi217N-linked (GlcNAc...) asparagine2 Publications1
Modified residuei306Phosphoserine; by FAM20CCombined sources1 Publication1
Modified residuei403PhosphoserineBy similarity1
Modified residuei404N6-succinyllysineBy similarity1
Glycosylationi445N-linked (GlcNAc...) asparagine2 Publications1
Modified residuei447PhosphoserineCombined sources1
Modified residuei479N6-acetyllysineBy similarity1
Glycosylationi481N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi502N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei633N6-succinyllysineBy similarity1
Modified residuei786PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP14625
PaxDbiP14625
PeptideAtlasiP14625
PRIDEiP14625
ProteomicsDBi53067
TopDownProteomicsiP14625

2D gel databases

DOSAC-COBS-2DPAGEiP14625
OGPiP14625
REPRODUCTION-2DPAGEiIPI00027230

PTM databases

GlyConnecti1206
iPTMnetiP14625
PhosphoSitePlusiP14625
SwissPalmiP14625

Miscellaneous databases

PMAP-CutDBiP14625

Expressioni

Gene expression databases

BgeeiENSG00000166598 Expressed in 239 organ(s), highest expression level in testis
CleanExiHS_HSP90B1
ExpressionAtlasiP14625 baseline and differential
GenevisibleiP14625 HS

Organism-specific databases

HPAiCAB005224
HPA003901
HPA008424

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP (By similarity). Interacts with TLR4 and TLR9, but not with TLR3. Interacts with MZB1 in a calcium-dependent manner (By similarity). Interacts with METTL23.By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113036, 175 interactors
CORUMiP14625
DIPiDIP-36060N
IntActiP14625, 87 interactors
MINTiP14625
STRINGi9606.ENSP00000299767

Chemistry databases

BindingDBiP14625

Structurei

Secondary structure

1803
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP14625
SMRiP14625
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi800 – 803Prevents secretion from ER4

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0020 Eukaryota
COG0326 LUCA
GeneTreeiENSGT00920000149016
HOGENOMiHOG000031988
HOVERGENiHBG007374
InParanoidiP14625
KOiK09487
OMAiSDHEIFL
OrthoDBiEOG091G0270
PhylomeDBiP14625
TreeFamiTF105969

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 2 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00298 HSP90, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

P14625-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRALWVLGLC CVLLTFGSVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ
60 70 80 90 100
REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF
110 120 130 140 150
LRELISNASD ALDKIRLISL TDENALSGNE ELTVKIKCDK EKNLLHVTDT
160 170 180 190 200
GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY
210 220 230 240 250
SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV
260 270 280 290 300
LKEEASDYLE LDTIKNLVKK YSQFINFPIY VWSSKTETVE EPMEEEEAAK
310 320 330 340 350
EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV
360 370 380 390 400
EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE
410 420 430 440 450
YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET
460 470 480 490 500
LQQHKLLKVI RKKLVRKTLD MIKKIADDKY NDTFWKEFGT NIKLGVIEDH
510 520 530 540 550
SNRTRLAKLL RFQSSHHPTD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE
560 570 580 590 600
SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE
610 620 630 640 650
SEKTKESREA VEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS
660 670 680 690 700
QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR
710 720 730 740 750
IKEDEDDKTV LDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID
760 770 780 790 800
PDAKVEEEPE EEPEETAEDT TEDTEQDEDE EMDVGTDEEE ETAKESTAEK

DEL
Length:803
Mass (Da):92,469
Last modified:April 1, 1990 - v1
Checksum:i9BF6705A7A2ED0D0
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q96GW1Q96GW1_HUMAN
Endoplasmin
HSP90B1
315Annotation score:
A0A1W2PRR1A0A1W2PRR1_HUMAN
Endoplasmin
HSP90B1
315Annotation score:
H0YIV0H0YIV0_HUMAN
Endoplasmin
HSP90B1
147Annotation score:
F8W026F8W026_HUMAN
Endoplasmin
HSP90B1
48Annotation score:
F8VPC7F8VPC7_HUMAN
Endoplasmin
HSP90B1
53Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188T → S in AAK74072 (Ref. 4) Curated1
Sequence conflicti419T → P in AAK74072 (Ref. 4) Curated1
Sequence conflicti803L → F in AAK74072 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15187 mRNA Translation: CAA33261.1
M33716 Genomic DNA Translation: AAA68201.1
BC066656 mRNA Translation: AAH66656.1
M26596 Genomic DNA Translation: AAA58621.1
AY040226 mRNA Translation: AAK74072.1
CCDSiCCDS9094.1
PIRiA35954
RefSeqiNP_003290.1, NM_003299.2
UniGeneiHs.192374

Genome annotation databases

EnsembliENST00000299767; ENSP00000299767; ENSG00000166598
GeneIDi7184
KEGGihsa:7184

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15187 mRNA Translation: CAA33261.1
M33716 Genomic DNA Translation: AAA68201.1
BC066656 mRNA Translation: AAH66656.1
M26596 Genomic DNA Translation: AAA58621.1
AY040226 mRNA Translation: AAK74072.1
CCDSiCCDS9094.1
PIRiA35954
RefSeqiNP_003290.1, NM_003299.2
UniGeneiHs.192374

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NH9X-ray2.77A69-337[»]
ProteinModelPortaliP14625
SMRiP14625
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113036, 175 interactors
CORUMiP14625
DIPiDIP-36060N
IntActiP14625, 87 interactors
MINTiP14625
STRINGi9606.ENSP00000299767

Chemistry databases

BindingDBiP14625
ChEMBLiCHEMBL1075323
DrugBankiDB02935 1-Methoxy-2-(2-Methoxyethoxy)Ethane
DB02103 2-Chlorodideoxyadenosine
DB03719 N-Ethyl-5'-Carboxamido Adenosine
DB03758 Radicicol
DB00615 Rifabutin
GuidetoPHARMACOLOGYi2904

PTM databases

GlyConnecti1206
iPTMnetiP14625
PhosphoSitePlusiP14625
SwissPalmiP14625

Polymorphism and mutation databases

DMDMi119360

2D gel databases

DOSAC-COBS-2DPAGEiP14625
OGPiP14625
REPRODUCTION-2DPAGEiIPI00027230

Proteomic databases

EPDiP14625
PaxDbiP14625
PeptideAtlasiP14625
PRIDEiP14625
ProteomicsDBi53067
TopDownProteomicsiP14625

Protocols and materials databases

DNASUi7184
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299767; ENSP00000299767; ENSG00000166598
GeneIDi7184
KEGGihsa:7184

Organism-specific databases

CTDi7184
DisGeNETi7184
EuPathDBiHostDB:ENSG00000166598.12
GeneCardsiHSP90B1
H-InvDBiHIX0056796
HGNCiHGNC:12028 HSP90B1
HPAiCAB005224
HPA003901
HPA008424
MIMi191175 gene
neXtProtiNX_P14625
OpenTargetsiENSG00000166598
PharmGKBiPA36705
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0020 Eukaryota
COG0326 LUCA
GeneTreeiENSGT00920000149016
HOGENOMiHOG000031988
HOVERGENiHBG007374
InParanoidiP14625
KOiK09487
OMAiSDHEIFL
OrthoDBiEOG091G0270
PhylomeDBiP14625
TreeFamiTF105969

Enzyme and pathway databases

ReactomeiR-HSA-1679131 Trafficking and processing of endosomal TLR
R-HSA-3000480 Scavenging by Class A Receptors
R-HSA-381183 ATF6 (ATF6-alpha) activates chaperone genes
R-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
R-HSA-8957275 Post-translational protein phosphorylation

Miscellaneous databases

ChiTaRSiHSP90B1 human
GeneWikiiHSP90B1
GenomeRNAii7184
PMAP-CutDBiP14625
PROiPR:P14625
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166598 Expressed in 239 organ(s), highest expression level in testis
CleanExiHS_HSP90B1
ExpressionAtlasiP14625 baseline and differential
GenevisibleiP14625 HS

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 2 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00298 HSP90, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENPL_HUMAN
AccessioniPrimary (citable) accession number: P14625
Secondary accession number(s): Q96A97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 10, 2018
This is version 219 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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