UniProtKB - P14585 (LIN12_CAEEL)
Protein
Protein lin-12
Gene
lin-12
Organism
Caenorhabditis elegans
Status
Functioni
Involved in several cell fate decisions that require cell-cell interactions. It is possible that lin-12 encodes a membrane-bound receptor for a signal that enables expression of the ventral uterine precursor cell fate. Activity in cell fate decisions and tumorigenesis is negatively regulated by sel-10. Functions in uterine cells to promote basement membrane mobility during tissue remodeling (PubMed:27661254).3 Publications
GO - Molecular functioni
- calcium ion binding Source: InterPro
- Notch binding Source: GO_Central
- RNA polymerase II transcription factor binding Source: WormBase
- transmembrane signaling receptor activity Source: WormBase
GO - Biological processi
- cell-cell signaling involved in cell fate commitment Source: WormBase
- cell fate specification Source: WormBase
- dauer exit Source: WormBase
- nematode larval development Source: WormBase
- Notch signaling pathway Source: WormBase
- oviposition Source: WormBase
- positive regulation of mesodermal cell fate specification Source: UniProtKB
- regulation of cell fate specification Source: UniProtKB
- regulation of mesodermal cell fate specification Source: UniProtKB
- regulation of vulval development Source: UniProtKB
- vulval development Source: WormBase
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation |
Enzyme and pathway databases
Reactomei | R-CEL-1912420, Pre-NOTCH Processing in Golgi R-CEL-9013700, NOTCH4 Activation and Transmission of Signal to the Nucleus R-CEL-9604323, Negative regulation of NOTCH4 signaling |
SignaLinki | P14585 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein lin-12Alternative name(s): Abnormal cell lineage protein 12 |
Gene namesi | Name:lin-12Imported ORF Names:R107.8Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | R107.8 ; CE00274 ; WBGene00003001 ; lin-12 |
Subcellular locationi
Plasma membrane
- Apical cell membrane 1 Publication; Single-pass type I membrane protein Sequence analysis
Note: Localizes to the apical cell membrane of vulval precursor cells.1 Publication
Nucleus
- nucleus Source: WormBase
Plasma Membrane
- apical plasma membrane Source: WormBase
- plasma membrane Source: WormBase
Other locations
- integral component of membrane Source: UniProtKB-KW
- RNA polymerase II transcription regulator complex Source: WormBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 16 – 908 | ExtracellularSequence analysisAdd BLAST | 893 | |
Transmembranei | 909 – 931 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 932 – 1429 | CytoplasmicSequence analysisAdd BLAST | 498 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
RNAi-mediated knockdown in cells of the pi uterine cell lineage results in impaired basement membrane mobility.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | Sequence analysisAdd BLAST | 15 | |
ChainiPRO_0000007634 | 16 – 1429 | Protein lin-12Add BLAST | 1414 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 24 ↔ 35 | By similarity | ||
Disulfide bondi | 29 ↔ 49 | By similarity | ||
Glycosylationi | 41 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 51 ↔ 60 | By similarity | ||
Disulfide bondi | 118 ↔ 129 | By similarity | ||
Disulfide bondi | 123 ↔ 138 | By similarity | ||
Disulfide bondi | 140 ↔ 149 | By similarity | ||
Disulfide bondi | 156 ↔ 169 | By similarity | ||
Disulfide bondi | 163 ↔ 178 | By similarity | ||
Glycosylationi | 165 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 180 ↔ 189 | By similarity | ||
Glycosylationi | 194 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 205 ↔ 227 | By similarity | ||
Disulfide bondi | 221 ↔ 234 | By similarity | ||
Disulfide bondi | 236 ↔ 245 | By similarity | ||
Disulfide bondi | 254 ↔ 264 | By similarity | ||
Disulfide bondi | 259 ↔ 273 | By similarity | ||
Disulfide bondi | 275 ↔ 284 | By similarity | ||
Disulfide bondi | 291 ↔ 302 | By similarity | ||
Disulfide bondi | 296 ↔ 311 | By similarity | ||
Disulfide bondi | 313 ↔ 322 | By similarity | ||
Disulfide bondi | 327 ↔ 339 | By similarity | ||
Disulfide bondi | 334 ↔ 351 | By similarity | ||
Disulfide bondi | 353 ↔ 362 | By similarity | ||
Disulfide bondi | 369 ↔ 381 | By similarity | ||
Disulfide bondi | 375 ↔ 390 | By similarity | ||
Glycosylationi | 378 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 392 ↔ 401 | By similarity | ||
Disulfide bondi | 408 ↔ 419 | By similarity | ||
Disulfide bondi | 413 ↔ 429 | By similarity | ||
Disulfide bondi | 431 ↔ 440 | By similarity | ||
Disulfide bondi | 462 ↔ 475 | By similarity | ||
Disulfide bondi | 469 ↔ 480 | By similarity | ||
Disulfide bondi | 482 ↔ 491 | By similarity | ||
Disulfide bondi | 507 ↔ 518 | By similarity | ||
Disulfide bondi | 512 ↔ 529 | By similarity | ||
Glycosylationi | 515 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 531 ↔ 540 | By similarity | ||
Disulfide bondi | 547 ↔ 558 | By similarity | ||
Disulfide bondi | 552 ↔ 567 | By similarity | ||
Disulfide bondi | 569 ↔ 578 | By similarity | ||
Disulfide bondi | 586 ↔ 597 | By similarity | ||
Disulfide bondi | 591 ↔ 607 | By similarity | ||
Disulfide bondi | 609 ↔ 618 | By similarity | ||
Glycosylationi | 623 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 638 ↔ 661 | By similarity | ||
Disulfide bondi | 643 ↔ 656 | By similarity | ||
Disulfide bondi | 652 ↔ 668 | By similarity | ||
Disulfide bondi | 678 ↔ 702 | By similarity | ||
Disulfide bondi | 684 ↔ 697 | By similarity | ||
Disulfide bondi | 693 ↔ 709 | By similarity | ||
Disulfide bondi | 716 ↔ 742 | By similarity | ||
Disulfide bondi | 724 ↔ 737 | By similarity | ||
Disulfide bondi | 733 ↔ 749 | By similarity | ||
Glycosylationi | 751 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 754 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 900 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P14585 |
PRIDEi | P14585 |
PTM databases
iPTMneti | P14585 |
Expressioni
Gene expression databases
Bgeei | WBGene00003001, Expressed in material anatomical entity and 4 other tissues |
Interactioni
Subunit structurei
Interacts with sel-10.
1 PublicationBinary interactionsi
P14585
With | #Exp. | IntAct |
---|---|---|
sel-10 [Q93794] | 3 | EBI-326049,EBI-323098 |
GO - Molecular functioni
- Notch binding Source: GO_Central
- RNA polymerase II transcription factor binding Source: WormBase
Protein-protein interaction databases
BioGRIDi | 41481, 19 interactors |
ComplexPortali | CPX-3152, CSL-Notch-Mastermind transcription factor complex |
DIPi | DIP-25208N |
ELMi | P14585 |
IntActi | P14585, 5 interactors |
MINTi | P14585 |
STRINGi | 6239.R107.8 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14585 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14585 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 61 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 114 – 150 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 152 – 190 | EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 201 – 246 | EGF-like 4PROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 250 – 285 | EGF-like 5PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 287 – 323 | EGF-like 6PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 323 – 363 | EGF-like 7PROSITE-ProRule annotationAdd BLAST | 41 | |
Domaini | 365 – 402 | EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 404 – 441 | EGF-like 9PROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 449 – 492 | EGF-like 10PROSITE-ProRule annotationAdd BLAST | 44 | |
Domaini | 503 – 541 | EGF-like 11PROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 543 – 579 | EGF-like 12PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 582 – 619 | EGF-like 13PROSITE-ProRule annotationAdd BLAST | 38 | |
Repeati | 638 – 674 | LNR 1Add BLAST | 37 | |
Repeati | 678 – 709 | LNR 2Add BLAST | 32 | |
Repeati | 716 – 754 | LNR 3Add BLAST | 39 | |
Repeati | 1093 – 1122 | ANK 1Add BLAST | 30 | |
Repeati | 1126 – 1158 | ANK 2Add BLAST | 33 | |
Repeati | 1162 – 1194 | ANK 3Add BLAST | 33 | |
Repeati | 1206 – 1236 | ANK 4Add BLAST | 31 | |
Repeati | 1240 – 1269 | ANK 5Add BLAST | 30 |
Keywords - Domaini
ANK repeat, EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00980000198606 |
HOGENOMi | CLU_005264_0_0_1 |
InParanoidi | P14585 |
OMAi | TCIDSPL |
OrthoDBi | 7525at2759 |
PhylomeDBi | P14585 |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
IDEALi | IID50027 |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR001881, EGF-like_Ca-bd_dom IPR013032, EGF-like_CS IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf IPR035993, Notch-like_dom_sf IPR000800, Notch_dom IPR010660, Notch_NOD_dom IPR011656, Notch_NODP_dom |
Pfami | View protein in Pfam PF00023, Ank, 1 hit PF12796, Ank_2, 1 hit PF00008, EGF, 6 hits PF07645, EGF_CA, 1 hit PF12661, hEGF, 3 hits PF06816, NOD, 1 hit PF07684, NODP, 1 hit PF00066, Notch, 3 hits |
PRINTSi | PR01452, LNOTCHREPEAT |
SMARTi | View protein in SMART SM00248, ANK, 6 hits SM00181, EGF, 13 hits SM00179, EGF_CA, 9 hits SM00004, NL, 3 hits SM01338, NOD, 1 hit SM01339, NODP, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF57184, SSF57184, 1 hit SSF90193, SSF90193, 3 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 3 hits PS00010, ASX_HYDROXYL, 3 hits PS00022, EGF_1, 12 hits PS01186, EGF_2, 11 hits PS50026, EGF_3, 13 hits PS01187, EGF_CA, 2 hits PS50258, LNR, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P14585-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIPTICFLF LLISLSKSLH IGSCLGLICG RNGHCHAGPV NGTQTSYWCR
60 70 80 90 100
CDEGFGGEYC EQQCDVSKCG ADEKCVFDKD YRMETCVCKD CDINGNSLLK
110 120 130 140 150
PSCPSGYGGD DCKTQGWCYP SVCMNGGQCI GAGNRAKCAC PDGFKGERCE
160 170 180 190 200
LDVNECEENK NACGNRSTCM NTLGTYICVC PQGFLPPDCL KPGNTSTVEF
210 220 230 240 250
KQPVCFLEIS ADHPDGRSMY CQNGGFCDKA SSKCQCPPGY HGSTCELLEK
260 270 280 290 300
EDSCASNPCS HGVCISFSGG FQCICDDGYS GSYCQEGKDN CVNNKCEAGS
310 320 330 340 350
KCINGVNSYF CDCPPERTGP YCEKMDCSAI PDICNHGTCI DSPLSEKAFE
360 370 380 390 400
CQCEPGYEGI LCEQDKNECL SENMCLNNGT CVNLPGSFRC DCARGFGGKW
410 420 430 440 450
CDEPLNMCQD FHCENDGTCM HTSDHSPVCQ CKNGFIGKRC EKECPIGFGG
460 470 480 490 500
VRCDLRLEIG ICSRQGGKCF NGGKCLSGFC VCPPDFTGNQ CEVNRKNGKS
510 520 530 540 550
SLSENLCLSD PCMNNATCID VDAHIGYACI CKQGFEGDIC ERHKDLCLEN
560 570 580 590 600
PCSNGGVCHQ HRESFSCDCP PGFYGNGCEQ EKMFRCLKST CQNGGVCINE
610 620 630 640 650
EEKGRKCECS YGFSGARCEE KINLTGFTEK DSLLRSVCEK RKCSERANDG
660 670 680 690 700
NCDADCNYAA CKFDGGDCSG KREPFSKCRY GNMCADFFAN GVCNQACNNE
710 720 730 740 750
ECLYDGMDCL PAVVRCPVKI REHCASRFAN GICDPECNTN GCGFDGGDCD
760 770 780 790 800
NETNATIITN IRITVQMDPK EFQVTGGQSL MEISSALRVT VRIQRDEEGP
810 820 830 840 850
LVFQWNGESE MDRVKMNERQ LTEQHVLSTS ISRKIKRSAT NIGVVVYLEV
860 870 880 890 900
QENCDTGKCL YKDAQSVVDS ISARLAKKGI DSFGIPISEA LVAEPRKSGN
910 920 930 940 950
NTGFLSWNAL LLIGAGCLIV MVVLMLGALP GNRTRKRRMI NASVWMPPME
960 970 980 990 1000
NEEKNRKNHQ SITSSQHSLL EASYDGYIKR QRNELQHYSL YPNPQGYGNG
1010 1020 1030 1040 1050
NDFLGDFNHT NLQIPTEPEP ESPIKLHTEA AGSYAITEPI TRESVNIIDP
1060 1070 1080 1090 1100
RHNRTVLHWI ASNSSAEKSE DLIVHEAKEC IAAGADVNAM DCDENTPLML
1110 1120 1130 1140 1150
AVLARRRRLV AYLMKAGADP TIYNKSERSA LHQAAANRDF GMMVYMLNST
1160 1170 1180 1190 1200
KLKGDIEELD RNGMTALMIV AHNEGRDQVA SAKLLVEKGA KVDYDGAARK
1210 1220 1230 1240 1250
DSEKYKGRTA LHYAAQVSNM PIVKYLVGEK GSNKDKQDED GKTPIMLAAQ
1260 1270 1280 1290 1300
EGRIEVVMYL IQQGASVEAV DATDHTARQL AQANNHHNIV DIFDRCRPER
1310 1320 1330 1340 1350
EYSMDLHIQH THQPQPSRKV TRAPKKQTSR SKKESASNSR DSTHLTPPPS
1360 1370 1380 1390 1400
DGSTSTPSPQ HFMNTTHTTP TSLNYLSPEY QTEAGSSEAF QPQCGAFGNG
1410 1420
EMWYTRASTS YTQMQNEPMT RYSEPAHYF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12069 Genomic DNA Translation: AAA70191.1 BX284603 Genomic DNA Translation: CAA78474.1 |
PIRi | S06434 |
RefSeqi | NP_499007.1, NM_066606.3 |
Genome annotation databases
EnsemblMetazoai | R107.8.1; R107.8.1; WBGene00003001 |
GeneIDi | 176282 |
KEGGi | cel:CELE_R107.8 |
UCSCi | R107.8, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12069 Genomic DNA Translation: AAA70191.1 BX284603 Genomic DNA Translation: CAA78474.1 |
PIRi | S06434 |
RefSeqi | NP_499007.1, NM_066606.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FO1 | X-ray | 3.12 | E | 931-1301 | [»] | |
3BRD | X-ray | 2.21 | D | 930-957 | [»] | |
3BRF | X-ray | 2.47 | D | 938-950 | [»] | |
SMRi | P14585 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 41481, 19 interactors |
ComplexPortali | CPX-3152, CSL-Notch-Mastermind transcription factor complex |
DIPi | DIP-25208N |
ELMi | P14585 |
IntActi | P14585, 5 interactors |
MINTi | P14585 |
STRINGi | 6239.R107.8 |
PTM databases
iPTMneti | P14585 |
Proteomic databases
PaxDbi | P14585 |
PRIDEi | P14585 |
Genome annotation databases
EnsemblMetazoai | R107.8.1; R107.8.1; WBGene00003001 |
GeneIDi | 176282 |
KEGGi | cel:CELE_R107.8 |
UCSCi | R107.8, c. elegans |
Organism-specific databases
CTDi | 176282 |
WormBasei | R107.8 ; CE00274 ; WBGene00003001 ; lin-12 |
Phylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00980000198606 |
HOGENOMi | CLU_005264_0_0_1 |
InParanoidi | P14585 |
OMAi | TCIDSPL |
OrthoDBi | 7525at2759 |
PhylomeDBi | P14585 |
Enzyme and pathway databases
Reactomei | R-CEL-1912420, Pre-NOTCH Processing in Golgi R-CEL-9013700, NOTCH4 Activation and Transmission of Signal to the Nucleus R-CEL-9604323, Negative regulation of NOTCH4 signaling |
SignaLinki | P14585 |
Miscellaneous databases
EvolutionaryTracei | P14585 |
PROi | PR:P14585 |
Gene expression databases
Bgeei | WBGene00003001, Expressed in material anatomical entity and 4 other tissues |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
IDEALi | IID50027 |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR001881, EGF-like_Ca-bd_dom IPR013032, EGF-like_CS IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf IPR035993, Notch-like_dom_sf IPR000800, Notch_dom IPR010660, Notch_NOD_dom IPR011656, Notch_NODP_dom |
Pfami | View protein in Pfam PF00023, Ank, 1 hit PF12796, Ank_2, 1 hit PF00008, EGF, 6 hits PF07645, EGF_CA, 1 hit PF12661, hEGF, 3 hits PF06816, NOD, 1 hit PF07684, NODP, 1 hit PF00066, Notch, 3 hits |
PRINTSi | PR01452, LNOTCHREPEAT |
SMARTi | View protein in SMART SM00248, ANK, 6 hits SM00181, EGF, 13 hits SM00179, EGF_CA, 9 hits SM00004, NL, 3 hits SM01338, NOD, 1 hit SM01339, NODP, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF57184, SSF57184, 1 hit SSF90193, SSF90193, 3 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 3 hits PS00010, ASX_HYDROXYL, 3 hits PS00022, EGF_1, 12 hits PS01186, EGF_2, 11 hits PS50026, EGF_3, 13 hits PS01187, EGF_CA, 2 hits PS50258, LNR, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LIN12_CAEEL | |
Accessioni | P14585Primary (citable) accession number: P14585 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references