UniProtKB - P14576 (SRP54_MOUSE)
Protein
Signal recognition particle 54 kDa protein
Gene
Srp54
Organism
Mus musculus (Mouse)
Status
Functioni
Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). Plays a role in proliferation and differentiation of granulocytic cells, neutrophils migration capacity and exocrine pancreas development.By similarity
Catalytic activityi
- This reaction proceeds in the forwardBy similarity direction.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 108 – 115 | GTPBy similarity | 8 | |
Nucleotide bindingi | 190 – 194 | GTPBy similarity | 5 | |
Nucleotide bindingi | 248 – 251 | GTPBy similarity | 4 |
GO - Molecular functioni
- 7S RNA binding Source: MGI
- endoplasmic reticulum signal peptide binding Source: MGI
- GDP binding Source: MGI
- GTPase activity Source: UniProtKB
- GTP binding Source: MGI
- ribonucleoprotein complex binding Source: MGI
GO - Biological processi
- exocrine pancreas development Source: UniProtKB
- granulocyte differentiation Source: UniProtKB
- neutrophil chemotaxis Source: UniProtKB
- SRP-dependent cotranslational protein targeting to membrane Source: MGI
- SRP-dependent cotranslational protein targeting to membrane, translocation Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Ribonucleoprotein, RNA-binding |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1799339, SRP-dependent cotranslational protein targeting to membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Signal recognition particle 54 kDa protein (EC:3.6.5.-By similarity)Short name: SRP54 |
Gene namesi | Name:Srp54 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346087, Srp54 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Nucleus
- Nucleus speckle By similarity
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- signal recognition particle, endoplasmic reticulum targeting Source: MGI
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Nucleus, Signal recognition particlePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101194 | 1 – 504 | Signal recognition particle 54 kDa proteinAdd BLAST | 504 |
Proteomic databases
EPDi | P14576 |
jPOSTi | P14576 |
MaxQBi | P14576 |
PaxDbi | P14576 |
PeptideAtlasi | P14576 |
PRIDEi | P14576 |
PTM databases
iPTMneti | P14576 |
PhosphoSitePlusi | P14576 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000073079, Expressed in retinal neural layer and 53 other tissues |
ExpressionAtlasi | P14576, baseline and differential |
Genevisiblei | P14576, MM |
Interactioni
Subunit structurei
Interacts with RNPS1 (By similarity). Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.
By similarityProtein-protein interaction databases
BioGRIDi | 204879, 2 interactors |
IntActi | P14576, 3 interactors |
MINTi | P14576 |
STRINGi | 10090.ENSMUSP00000106336 |
Miscellaneous databases
RNActi | P14576, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14576 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14576 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 295 | G-domainAdd BLAST | 295 | |
Regioni | 296 – 504 | M-domainAdd BLAST | 209 |
Domaini
Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0780, Eukaryota |
GeneTreei | ENSGT00550000074824 |
HOGENOMi | CLU_009301_6_1_1 |
InParanoidi | P14576 |
OMAi | RPNAVNQ |
OrthoDBi | 463152at2759 |
PhylomeDBi | P14576 |
TreeFami | TF106249 |
Family and domain databases
Gene3Di | 1.10.260.30, 1 hit 1.20.120.140, 1 hit |
HAMAPi | MF_00306, SRP54, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR036891, Signal_recog_part_SRP54_M_sf IPR013822, Signal_recog_particl_SRP54_hlx IPR004125, Signal_recog_particle_SRP54_M IPR022941, SRP54 IPR006325, SRP54_euk IPR000897, SRP54_GTPase_dom IPR042101, SRP54_N_sf |
PANTHERi | PTHR11564, PTHR11564, 1 hit |
Pfami | View protein in Pfam PF00448, SRP54, 1 hit PF02881, SRP54_N, 1 hit PF02978, SRP_SPB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00962, SRP54, 1 hit SM00963, SRP54_N, 1 hit |
SUPFAMi | SSF47446, SSF47446, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01425, SRP54_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00300, SRP54, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P14576-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK
60 70 80 90 100
QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK
110 120 130 140 150
QNVIMFVGLQ GSGKTTTCSK LAYYYQRKGW KTCLICADTF RAGAFDQLKQ
160 170 180 190 200
NATKARIPFY GSYTEMDPVI IASEGVEKFK NENFEIIIVD TSGRHKQEDS
210 220 230 240 250
LFEEMLQVSN AIQPDNIVYV MDASIGQACE AQAKAFKDKV DVASVIVTKL
260 270 280 290 300
DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI
310 320 330 340 350
EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG
360 370 380 390 400
MIPGFGTDFM SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP
410 420 430 440 450
GRIQRVARGS GVSTRDVQEL LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV
460 470 480 490 500
SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL QSMMRQFQQG AAGNMKGMMG
FNNM
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P809 | A0A1W2P809_MOUSE | Signal recognition particle 54A | Srp54b Srp54a | 89 | Annotation score: | ||
A0A1Y7VJJ0 | A0A1Y7VJJ0_MOUSE | Signal recognition particle 54A | Srp54a | 110 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 205 | M → R in CAA34386 (PubMed:2502718).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014953 | 86 – 103 | LVDPG…GKQNV → VKVYQHYESINSVLLSPF in isoform 2. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_014954 | 104 – 504 | Missing in isoform 2. 1 PublicationAdd BLAST | 401 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16319 mRNA Translation: CAA34386.1 AK010716 mRNA Translation: BAB27138.1 AK011928 mRNA Translation: BAB27921.1 AK136033 mRNA Translation: BAE22786.1 BC019683 mRNA Translation: AAH19683.1 |
CCDSi | CCDS36449.1 [P14576-1] CCDS49065.1 [P14576-1] |
PIRi | S05198 |
RefSeqi | NP_001093579.1, NM_001100109.1 [P14576-1] NP_036029.2, NM_011899.4 [P14576-1] |
Genome annotation databases
Ensembli | ENSMUST00000021407; ENSMUSP00000021407; ENSMUSG00000073079 [P14576-1] ENSMUST00000110708; ENSMUSP00000106336; ENSMUSG00000112449 [P14576-1] ENSMUST00000220578; ENSMUSP00000152764; ENSMUSG00000073079 [P14576-1] |
GeneIDi | 24067 665155 |
KEGGi | mmu:24067 mmu:665155 |
UCSCi | uc007nof.1, mouse [P14576-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16319 mRNA Translation: CAA34386.1 AK010716 mRNA Translation: BAB27138.1 AK011928 mRNA Translation: BAB27921.1 AK136033 mRNA Translation: BAE22786.1 BC019683 mRNA Translation: AAH19683.1 |
CCDSi | CCDS36449.1 [P14576-1] CCDS49065.1 [P14576-1] |
PIRi | S05198 |
RefSeqi | NP_001093579.1, NM_001100109.1 [P14576-1] NP_036029.2, NM_011899.4 [P14576-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RY1 | electron microscopy | 12.00 | W | 326-434 | [»] | |
1WGW | NMR | - | A | 4-85 | [»] | |
SMRi | P14576 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204879, 2 interactors |
IntActi | P14576, 3 interactors |
MINTi | P14576 |
STRINGi | 10090.ENSMUSP00000106336 |
PTM databases
iPTMneti | P14576 |
PhosphoSitePlusi | P14576 |
Proteomic databases
EPDi | P14576 |
jPOSTi | P14576 |
MaxQBi | P14576 |
PaxDbi | P14576 |
PeptideAtlasi | P14576 |
PRIDEi | P14576 |
Genome annotation databases
Ensembli | ENSMUST00000021407; ENSMUSP00000021407; ENSMUSG00000073079 [P14576-1] ENSMUST00000110708; ENSMUSP00000106336; ENSMUSG00000112449 [P14576-1] ENSMUST00000220578; ENSMUSP00000152764; ENSMUSG00000073079 [P14576-1] |
GeneIDi | 24067 665155 |
KEGGi | mmu:24067 mmu:665155 |
UCSCi | uc007nof.1, mouse [P14576-1] |
Organism-specific databases
CTDi | 24067 665155 |
MGIi | MGI:1346087, Srp54 |
Phylogenomic databases
eggNOGi | KOG0780, Eukaryota |
GeneTreei | ENSGT00550000074824 |
HOGENOMi | CLU_009301_6_1_1 |
InParanoidi | P14576 |
OMAi | RPNAVNQ |
OrthoDBi | 463152at2759 |
PhylomeDBi | P14576 |
TreeFami | TF106249 |
Enzyme and pathway databases
Reactomei | R-MMU-1799339, SRP-dependent cotranslational protein targeting to membrane |
Miscellaneous databases
BioGRID-ORCSi | 24067, 5 hits in 10 CRISPR screens 665155, 4 hits in 9 CRISPR screens |
ChiTaRSi | Srp54a, mouse |
EvolutionaryTracei | P14576 |
PROi | PR:P14576 |
RNActi | P14576, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000073079, Expressed in retinal neural layer and 53 other tissues |
ExpressionAtlasi | P14576, baseline and differential |
Genevisiblei | P14576, MM |
Family and domain databases
Gene3Di | 1.10.260.30, 1 hit 1.20.120.140, 1 hit |
HAMAPi | MF_00306, SRP54, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR036891, Signal_recog_part_SRP54_M_sf IPR013822, Signal_recog_particl_SRP54_hlx IPR004125, Signal_recog_particle_SRP54_M IPR022941, SRP54 IPR006325, SRP54_euk IPR000897, SRP54_GTPase_dom IPR042101, SRP54_N_sf |
PANTHERi | PTHR11564, PTHR11564, 1 hit |
Pfami | View protein in Pfam PF00448, SRP54, 1 hit PF02881, SRP54_N, 1 hit PF02978, SRP_SPB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00962, SRP54, 1 hit SM00963, SRP54_N, 1 hit |
SUPFAMi | SSF47446, SSF47446, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01425, SRP54_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00300, SRP54, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SRP54_MOUSE | |
Accessioni | P14576Primary (citable) accession number: P14576 Secondary accession number(s): Q3UWX8, Q9CWH7, Q9D008 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | August 16, 2005 | |
Last modified: | February 10, 2021 | |
This is version 189 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families