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Protein

Nidogen-1

Gene

NID1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • collagen binding Source: BHF-UCL
  • extracellular matrix structural constituent Source: BHF-UCL
  • laminin-1 binding Source: Ensembl
  • laminin binding Source: BHF-UCL
  • proteoglycan binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Biological processCell adhesion
LigandCalcium

Enzyme and pathway databases

ReactomeiR-HSA-1474228 Degradation of the extracellular matrix
R-HSA-3000157 Laminin interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Nidogen-1
Short name:
NID-1
Alternative name(s):
Entactin
Gene namesi
Name:NID1
Synonyms:NID
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000116962.14
HGNCiHGNC:7821 NID1
MIMi131390 gene
neXtProtiNX_P14543

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi4811
OpenTargetsiENSG00000116962
PharmGKBiPA31625

Chemistry databases

DrugBankiDB00013 Urokinase

Polymorphism and mutation databases

BioMutaiNID1
DMDMi251757450

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Add BLAST28
ChainiPRO_000000766929 – 1247Nidogen-1Add BLAST1219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei289SulfotyrosineSequence analysis1
Modified residuei296SulfotyrosineSequence analysis1
Disulfide bondi390 ↔ 403By similarity
Disulfide bondi397 ↔ 412By similarity
Disulfide bondi411 ↔ 618By similarity
Disulfide bondi414 ↔ 425By similarity
Disulfide bondi672 ↔ 685By similarity
Disulfide bondi679 ↔ 695By similarity
Disulfide bondi697 ↔ 708By similarity
Disulfide bondi714 ↔ 727By similarity
Disulfide bondi721 ↔ 736By similarity
Disulfide bondi738 ↔ 750By similarity
Disulfide bondi762 ↔ 777By similarity
Disulfide bondi769 ↔ 787By similarity
Disulfide bondi789 ↔ 800By similarity
Disulfide bondi806 ↔ 817By similarity
Disulfide bondi811 ↔ 826By similarity
Disulfide bondi828 ↔ 839By similarity
Disulfide bondi849 ↔ 878By similarity
Disulfide bondi889 ↔ 896By similarity
Disulfide bondi898 ↔ 919By similarity
Glycosylationi922O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi935O-linked (GalNAc...) threonineSequence analysis1
Disulfide bondi1212 ↔ 1223By similarity
Disulfide bondi1219 ↔ 1232By similarity
Disulfide bondi1234 ↔ 1243By similarity

Post-translational modificationi

N- and O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

EPDiP14543
MaxQBiP14543
PaxDbiP14543
PeptideAtlasiP14543
PRIDEiP14543
ProteomicsDBi53056
53057 [P14543-2]

PTM databases

iPTMnetiP14543
PhosphoSitePlusiP14543

Expressioni

Gene expression databases

BgeeiENSG00000116962 Expressed in 208 organ(s), highest expression level in endometrium
CleanExiHS_NID1
GenevisibleiP14543 HS

Organism-specific databases

HPAiCAB010044

Interactioni

Subunit structurei

Interacts with FBLN1 and LGALS3BP (By similarity). Interacts with PLXDC1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110876, 19 interactors
ComplexPortaliCPX-1265 Laminin111-nidogen complex
CPX-1282 Laminin211-nidogen complex
CPX-1285 Laminin221-nidogen complex
IntActiP14543, 10 interactors
MINTiP14543
STRINGi9606.ENSP00000264187

Structurei

3D structure databases

ProteinModelPortaliP14543
SMRiP14543
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini106 – 268NIDOPROSITE-ProRule annotationAdd BLAST163
Domaini386 – 426EGF-like 1PROSITE-ProRule annotationAdd BLAST41
Domaini430 – 667Nidogen G2 beta-barrelPROSITE-ProRule annotationAdd BLAST238
Domaini668 – 709EGF-like 2PROSITE-ProRule annotationAdd BLAST42
Domaini710 – 751EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini758 – 801EGF-like 4PROSITE-ProRule annotationAdd BLAST44
Domaini802 – 840EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini846 – 919Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST74
Repeati990 – 1032LDL-receptor class B 1Add BLAST43
Repeati1033 – 1075LDL-receptor class B 2Add BLAST43
Repeati1076 – 1120LDL-receptor class B 3Add BLAST45
Repeati1121 – 1162LDL-receptor class B 4Add BLAST42
Domaini1208 – 1244EGF-like 6PROSITE-ProRule annotationAdd BLAST37

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi702 – 704Cell attachment site3

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG1214 Eukaryota
ENOG410XR8N LUCA
GeneTreeiENSGT00910000144086
HOGENOMiHOG000072712
HOVERGENiHBG006498
InParanoidiP14543
KOiK06826
OMAiIGVAYDC
OrthoDBiEOG091G030P
PhylomeDBiP14543
TreeFamiTF320666

Family and domain databases

CDDicd00255 nidG2, 1 hit
cd00191 TY, 1 hit
Gene3Di2.120.10.30, 1 hit
4.10.800.10, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR026823 cEGF
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR024731 EGF_dom
IPR006605 G2_nidogen/fibulin_G2F
IPR009017 GFP
IPR009030 Growth_fac_rcpt_cys_sf
IPR000033 LDLR_classB_rpt
IPR003886 NIDO_dom
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF12662 cEGF, 1 hit
PF12947 EGF_3, 1 hit
PF07645 EGF_CA, 1 hit
PF07474 G2F, 1 hit
PF00058 Ldl_recept_b, 3 hits
PF06119 NIDO, 1 hit
PF00086 Thyroglobulin_1, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 6 hits
SM00179 EGF_CA, 4 hits
SM00682 G2F, 1 hit
SM00135 LY, 5 hits
SM00539 NIDO, 1 hit
SM00211 TY, 1 hit
SUPFAMiSSF54511 SSF54511, 1 hit
SSF57184 SSF57184, 2 hits
SSF57610 SSF57610, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 5 hits
PS50026 EGF_3, 5 hits
PS01187 EGF_CA, 2 hits
PS51120 LDLRB, 4 hits
PS51220 NIDO, 1 hit
PS50993 NIDOGEN_G2, 1 hit
PS00484 THYROGLOBULIN_1_1, 1 hit
PS51162 THYROGLOBULIN_1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P14543-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLASSSRIRA AWTRALLLPL LLAGPVGCLS RQELFPFGPG QGDLELEDGD
60 70 80 90 100
DFVSPALELS GALRFYDRSD IDAVYVTTNG IIATSEPPAK ESHPGLFPPT
110 120 130 140 150
FGAVAPFLAD LDTTDGLGKV YYREDLSPSI TQRAAECVHR GFPEISFQPS
160 170 180 190 200
SAVVVTWESV APYQGPSRDP DQKGKRNTFQ AVLASSDSSS YAIFLYPEDG
210 220 230 240 250
LQFHTTFSKK ENNQVPAVVA FSQGSVGFLW KSNGAYNIFA NDRESVENLA
260 270 280 290 300
KSSNSGQQGV WVFEIGSPAT TNGVVPADVI LGTEDGAEYD DEDEDYDLAT
310 320 330 340 350
TRLGLEDVGT TPFSYKALRR GGADTYSVPS VLSPRRAATE RPLGPPTERT
360 370 380 390 400
RSFQLAVETF HQQHPQVIDV DEVEETGVVF SYNTDSRQTC ANNRHQCSVH
410 420 430 440 450
AECRDYATGF CCSCVAGYTG NGRQCVAEGS PQRVNGKVKG RIFVGSSQVP
460 470 480 490 500
IVFENTDLHS YVVMNHGRSY TAISTIPETV GYSLLPLAPV GGIIGWMFAV
510 520 530 540 550
EQDGFKNGFS ITGGEFTRQA EVTFVGHPGN LVIKQRFSGI DEHGHLTIDT
560 570 580 590 600
ELEGRVPQIP FGSSVHIEPY TELYHYSTSV ITSSSTREYT VTEPERDGAS
610 620 630 640 650
PSRIYTYQWR QTITFQECVH DDSRPALPST QQLSVDSVFV LYNQEEKILR
660 670 680 690 700
YALSNSIGPV REGSPDALQN PCYIGTHGCD TNAACRPGPR TQFTCECSIG
710 720 730 740 750
FRGDGRTCYD IDECSEQPSV CGSHTICNNH PGTFRCECVE GYQFSDEGTC
760 770 780 790 800
VAVVDQRPIN YCETGLHNCD IPQRAQCIYT GGSSYTCSCL PGFSGDGQAC
810 820 830 840 850
QDVDECQPSR CHPDAFCYNT PGSFTCQCKP GYQGDGFRCV PGEVEKTRCQ
860 870 880 890 900
HEREHILGAA GATDPQRPIP PGLFVPECDA HGHYAPTQCH GSTGYCWCVD
910 920 930 940 950
RDGREVEGTR TRPGMTPPCL STVAPPIHQG PAVPTAVIPL PPGTHLLFAQ
960 970 980 990 1000
TGKIERLPLE GNTMRKTEAK AFLHVPAKVI IGLAFDCVDK MVYWTDITEP
1010 1020 1030 1040 1050
SIGRASLHGG EPTTIIRQDL GSPEGIAVDH LGRNIFWTDS NLDRIEVAKL
1060 1070 1080 1090 1100
DGTQRRVLFE TDLVNPRGIV TDSVRGNLYW TDWNRDNPKI ETSYMDGTNR
1110 1120 1130 1140 1150
RILVQDDLGL PNGLTFDAFS SQLCWVDAGT NRAECLNPSQ PSRRKALEGL
1160 1170 1180 1190 1200
QYPFAVTSYG KNLYFTDWKM NSVVALDLAI SKETDAFQPH KQTRLYGITT
1210 1220 1230 1240
ALSQCPQGHN YCSVNNGGCT HLCLATPGSR TCRCPDNTLG VDCIEQK
Length:1,247
Mass (Da):136,377
Last modified:July 7, 2009 - v3
Checksum:iFCC839416525CD48
GO
Isoform 2 (identifier: P14543-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     710-842: Missing.

Show »
Length:1,114
Mass (Da):122,017
Checksum:i177A25689611C3BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33 – 34EL → SS in CAA57709 (PubMed:7557988).Curated2
Sequence conflicti37 – 42FGPGQG → SAPDR in CAA57709 (PubMed:7557988).Curated6
Sequence conflicti653L → F in AAA59932 (PubMed:2574658).Curated1
Sequence conflicti653L → F in CAA57709 (PubMed:7557988).Curated1
Sequence conflicti1115T → H in AAA59932 (PubMed:2574658).Curated1
Sequence conflicti1115T → H in CAA57709 (PubMed:7557988).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05576031R → L. Corresponds to variant dbSNP:rs2071529Ensembl.1
Natural variantiVAR_05812360S → R1 PublicationCorresponds to variant dbSNP:rs17857302Ensembl.1
Natural variantiVAR_024264246V → I4 PublicationsCorresponds to variant dbSNP:rs10733133Ensembl.1
Natural variantiVAR_055761302R → H. Corresponds to variant dbSNP:rs16833183Ensembl.1
Natural variantiVAR_055762335R → H. Corresponds to variant dbSNP:rs34406281Ensembl.1
Natural variantiVAR_055763387R → H. Corresponds to variant dbSNP:rs16833154Ensembl.1
Natural variantiVAR_021904669Q → R. Corresponds to variant dbSNP:rs3738534Ensembl.1
Natural variantiVAR_058124807Q → H1 PublicationCorresponds to variant dbSNP:rs3738531Ensembl.1
Natural variantiVAR_055764970K → E. Corresponds to variant dbSNP:rs16833060Ensembl.1
Natural variantiVAR_0358351036F → S in a colorectal cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_0557651163L → V. Corresponds to variant dbSNP:rs16833032Ensembl.1
Natural variantiVAR_0557661226T → I. Corresponds to variant dbSNP:rs6662744Ensembl.1
Natural variantiVAR_0581251246Q → R3 PublicationsCorresponds to variant dbSNP:rs3213190Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017254710 – 842Missing in isoform 2. 2 PublicationsAdd BLAST133

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30269 mRNA Translation: AAA59932.1
X82245
, X84819, X84820, X84821, X84822, X84823, X84824, X84825, X84826, X84827, X84828, X84829, X84830, X84831, X84832, X84833, X84834, X84835, X84836, X84837 Genomic DNA Translation: CAA57709.1
AL122018 Genomic DNA No translation available.
AL139161 Genomic DNA No translation available.
BC045606 mRNA Translation: AAH45606.1
AB209448 mRNA Translation: BAD92685.1
M27445 mRNA Translation: AAA57261.1
CCDSiCCDS1608.1 [P14543-1]
PIRiA33322 MMHUND
RefSeqiNP_002499.2, NM_002508.2 [P14543-1]
UniGeneiHs.356624

Genome annotation databases

EnsembliENST00000264187; ENSP00000264187; ENSG00000116962 [P14543-1]
ENST00000366595; ENSP00000355554; ENSG00000116962 [P14543-2]
GeneIDi4811
KEGGihsa:4811
UCSCiuc001hxo.4 human [P14543-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30269 mRNA Translation: AAA59932.1
X82245
, X84819, X84820, X84821, X84822, X84823, X84824, X84825, X84826, X84827, X84828, X84829, X84830, X84831, X84832, X84833, X84834, X84835, X84836, X84837 Genomic DNA Translation: CAA57709.1
AL122018 Genomic DNA No translation available.
AL139161 Genomic DNA No translation available.
BC045606 mRNA Translation: AAH45606.1
AB209448 mRNA Translation: BAD92685.1
M27445 mRNA Translation: AAA57261.1
CCDSiCCDS1608.1 [P14543-1]
PIRiA33322 MMHUND
RefSeqiNP_002499.2, NM_002508.2 [P14543-1]
UniGeneiHs.356624

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NDXmodel-A941-1206[»]
ProteinModelPortaliP14543
SMRiP14543
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110876, 19 interactors
ComplexPortaliCPX-1265 Laminin111-nidogen complex
CPX-1282 Laminin211-nidogen complex
CPX-1285 Laminin221-nidogen complex
IntActiP14543, 10 interactors
MINTiP14543
STRINGi9606.ENSP00000264187

Chemistry databases

DrugBankiDB00013 Urokinase

PTM databases

iPTMnetiP14543
PhosphoSitePlusiP14543

Polymorphism and mutation databases

BioMutaiNID1
DMDMi251757450

Proteomic databases

EPDiP14543
MaxQBiP14543
PaxDbiP14543
PeptideAtlasiP14543
PRIDEiP14543
ProteomicsDBi53056
53057 [P14543-2]

Protocols and materials databases

DNASUi4811
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264187; ENSP00000264187; ENSG00000116962 [P14543-1]
ENST00000366595; ENSP00000355554; ENSG00000116962 [P14543-2]
GeneIDi4811
KEGGihsa:4811
UCSCiuc001hxo.4 human [P14543-1]

Organism-specific databases

CTDi4811
DisGeNETi4811
EuPathDBiHostDB:ENSG00000116962.14
GeneCardsiNID1
H-InvDBiHIX0023622
HGNCiHGNC:7821 NID1
HPAiCAB010044
MIMi131390 gene
neXtProtiNX_P14543
OpenTargetsiENSG00000116962
PharmGKBiPA31625
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1214 Eukaryota
ENOG410XR8N LUCA
GeneTreeiENSGT00910000144086
HOGENOMiHOG000072712
HOVERGENiHBG006498
InParanoidiP14543
KOiK06826
OMAiIGVAYDC
OrthoDBiEOG091G030P
PhylomeDBiP14543
TreeFamiTF320666

Enzyme and pathway databases

ReactomeiR-HSA-1474228 Degradation of the extracellular matrix
R-HSA-3000157 Laminin interactions

Miscellaneous databases

ChiTaRSiNID1 human
GeneWikiiEntactin
GenomeRNAii4811
PROiPR:P14543
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116962 Expressed in 208 organ(s), highest expression level in endometrium
CleanExiHS_NID1
GenevisibleiP14543 HS

Family and domain databases

CDDicd00255 nidG2, 1 hit
cd00191 TY, 1 hit
Gene3Di2.120.10.30, 1 hit
4.10.800.10, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR026823 cEGF
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR024731 EGF_dom
IPR006605 G2_nidogen/fibulin_G2F
IPR009017 GFP
IPR009030 Growth_fac_rcpt_cys_sf
IPR000033 LDLR_classB_rpt
IPR003886 NIDO_dom
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF12662 cEGF, 1 hit
PF12947 EGF_3, 1 hit
PF07645 EGF_CA, 1 hit
PF07474 G2F, 1 hit
PF00058 Ldl_recept_b, 3 hits
PF06119 NIDO, 1 hit
PF00086 Thyroglobulin_1, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 6 hits
SM00179 EGF_CA, 4 hits
SM00682 G2F, 1 hit
SM00135 LY, 5 hits
SM00539 NIDO, 1 hit
SM00211 TY, 1 hit
SUPFAMiSSF54511 SSF54511, 1 hit
SSF57184 SSF57184, 2 hits
SSF57610 SSF57610, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 5 hits
PS50026 EGF_3, 5 hits
PS01187 EGF_CA, 2 hits
PS51120 LDLRB, 4 hits
PS51220 NIDO, 1 hit
PS50993 NIDOGEN_G2, 1 hit
PS00484 THYROGLOBULIN_1_1, 1 hit
PS51162 THYROGLOBULIN_1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNID1_HUMAN
AccessioniPrimary (citable) accession number: P14543
Secondary accession number(s): Q14942
, Q59FL2, Q5TAF2, Q5TAF3, Q86XD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 7, 2009
Last modified: November 7, 2018
This is version 205 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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