UniProtKB - P14532 (CCPR_PSEAE)
Protein
Cytochrome c551 peroxidase
Gene
ccpA
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Catalyzes the peroxidative oxidation of azurin and cytochrome c551. Likely to provide protection against toxic peroxides.
Catalytic activityi
- EC:1.11.1.5
Cofactori
hemeNote: Binds 2 heme groups. Heme 1 is low-potential (-330 mV) with 2 His axial ligands and functions in the peroxidase reaction, while heme 2 is high potential (+320 mV) with His and Met axial ligands and functions to feed electrons from electron-shuttle proteins such as cytochrome c and azurin.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 74 | Heme 1; covalent | 1 | |
Binding sitei | 77 | Heme 1; covalent | 1 | |
Metal bindingi | 78 | Iron (heme 1 axial ligand) | 1 | |
Binding sitei | 220 | Heme 2; covalent | 1 | |
Binding sitei | 223 | Heme 2; covalent | 1 | |
Metal bindingi | 224 | Iron (heme 2 axial ligand) | 1 | |
Metal bindingi | 284 | Iron (heme 1 axial ligand) | 1 | |
Metal bindingi | 298 | Iron (heme 2 axial ligand) | 1 |
GO - Molecular functioni
- cytochrome-c peroxidase activity Source: PseudoCAP
- electron transfer activity Source: InterPro
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Electron transport, Transport |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-17891 PAER208964:G1FZ6-4681-MONOMER |
BRENDAi | 1.11.1.5, 5087 |
Protein family/group databases
PeroxiBasei | 3543, PaerDiHCcP |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome c551 peroxidase (EC:1.11.1.5)Short name: CCP Short name: Cytochrome c peroxidase |
Gene namesi | Name:ccpA Ordered Locus Names:PA4587 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA4587 |
Subcellular locationi
Other locations
Other locations
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 1 PublicationAdd BLAST | 23 | |
ChainiPRO_0000006598 | 24 – 346 | Cytochrome c551 peroxidase1 PublicationAdd BLAST | 323 |
Post-translational modificationi
Binds 2 heme groups per subunit.
Proteomic databases
PaxDbi | P14532 |
PRIDEi | P14532 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14532 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14532 |
Family & Domainsi
Domaini
Organized into two domains, each containing a covalent c-heme in a structure reminiscent of class 1 cytochromes c. The domains are related by a quasi-twofold axis. The domain interface contains a calcium-binding site with an unusual set of ligands.
Keywords - Domaini
SignalPhylogenomic databases
HOGENOMi | CLU_034652_1_1_6 |
InParanoidi | P14532 |
OMAi | KGPINAP |
PhylomeDBi | P14532 |
Family and domain databases
Gene3Di | 1.10.760.10, 2 hits |
InterProi | View protein in InterPro IPR009056, Cyt_c-like_dom IPR036909, Cyt_c-like_dom_sf IPR004852, Di-haem_cyt_c_peroxidsae IPR026259, MauG/Cytc_peroxidase |
Pfami | View protein in Pfam PF03150, CCP_MauG, 1 hit PF00034, Cytochrom_C, 1 hit |
PIRSFi | PIRSF000294, Cytochrome-c_peroxidase, 1 hit |
SUPFAMi | SSF46626, SSF46626, 2 hits |
PROSITEi | View protein in PROSITE PS51007, CYTC, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P14532-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQSSQLLPLG SLLLSFATPL AQADALHDQA SALFKPIPEQ VTELRGQPIS
60 70 80 90 100
EQQRELGKKL FFDPRLSRSH VLSCNTCHNV GTGGADNVPT SVGHGWQKGP
110 120 130 140 150
RNSPTVFNAV FNAAQFWDGR AKDLGEQAKG PIQNSVEMHS TPQLVEQTLG
160 170 180 190 200
SIPEYVDAFR KAFPKAGKPV SFDNMALAIE AYEATLVTPD SPFDLYLKGD
210 220 230 240 250
DKALDAQQKK GLKAFMDSGC SACHNGINLG GQAYFPFGLV KKPDASVLPS
260 270 280 290 300
GDKGRFAVTK TQSDEYVFRA APLRNVALTA PYFHSGQVWE LKDAVAIMGN
310 320 330 340
AQLGKQLAPD DVENIVAFLH SLSGKQPRVE YPLLPASTET TPRPAE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 86 | D → G (PubMed:8543038).Curated | 1 | |
Sequence conflicti | 93 | Missing (PubMed:8543038).Curated | 1 | |
Sequence conflicti | 96 | W → G (PubMed:8543038).Curated | 1 | |
Sequence conflicti | 102 – 120 | Missing (PubMed:8543038).CuratedAdd BLAST | 19 | |
Sequence conflicti | 137 | E → Q (PubMed:8543038).Curated | 1 | |
Sequence conflicti | 175 | M → V (PubMed:10984043).Curated | 1 | |
Sequence conflicti | 228 | Missing (PubMed:8543038).Curated | 1 | |
Sequence conflicti | 286 – 287 | GQ → QG (PubMed:8543038).Curated | 2 | |
Sequence conflicti | 290 | E → Q (PubMed:8543038).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 81 | G → D. | 1 | |
Natural varianti | 83 | G → D. | 1 | |
Natural varianti | 152 | I → V. | 1 | |
Natural varianti | 164 | P → A. | 1 | |
Natural varianti | 198 | K → I. | 1 | |
Natural varianti | 334 | L → W. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U23766 Genomic DNA Translation: AAC43378.1 AE004091 Genomic DNA Translation: AAG07975.1 |
PIRi | I53515 |
RefSeqi | NP_253277.1, NC_002516.2 WP_003094815.1, NZ_QZGE01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAG07975; AAG07975; PA4587 |
GeneIDi | 881021 |
KEGGi | pae:PA4587 |
PATRICi | fig|208964.12.peg.4801 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U23766 Genomic DNA Translation: AAC43378.1 AE004091 Genomic DNA Translation: AAG07975.1 |
PIRi | I53515 |
RefSeqi | NP_253277.1, NC_002516.2 WP_003094815.1, NZ_QZGE01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EB7 | X-ray | 2.40 | A | 24-346 | [»] | |
2VHD | X-ray | 2.30 | A/B | 24-346 | [»] | |
SMRi | P14532 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB03317, Ferroheme C |
Protein family/group databases
PeroxiBasei | 3543, PaerDiHCcP |
Proteomic databases
PaxDbi | P14532 |
PRIDEi | P14532 |
Genome annotation databases
EnsemblBacteriai | AAG07975; AAG07975; PA4587 |
GeneIDi | 881021 |
KEGGi | pae:PA4587 |
PATRICi | fig|208964.12.peg.4801 |
Organism-specific databases
PseudoCAPi | PA4587 |
Phylogenomic databases
HOGENOMi | CLU_034652_1_1_6 |
InParanoidi | P14532 |
OMAi | KGPINAP |
PhylomeDBi | P14532 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-17891 PAER208964:G1FZ6-4681-MONOMER |
BRENDAi | 1.11.1.5, 5087 |
Miscellaneous databases
EvolutionaryTracei | P14532 |
Family and domain databases
Gene3Di | 1.10.760.10, 2 hits |
InterProi | View protein in InterPro IPR009056, Cyt_c-like_dom IPR036909, Cyt_c-like_dom_sf IPR004852, Di-haem_cyt_c_peroxidsae IPR026259, MauG/Cytc_peroxidase |
Pfami | View protein in Pfam PF03150, CCP_MauG, 1 hit PF00034, Cytochrom_C, 1 hit |
PIRSFi | PIRSF000294, Cytochrome-c_peroxidase, 1 hit |
SUPFAMi | SSF46626, SSF46626, 2 hits |
PROSITEi | View protein in PROSITE PS51007, CYTC, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCPR_PSEAE | |
Accessioni | P14532Primary (citable) accession number: P14532 Secondary accession number(s): Q51369 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references