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UniProtKB - P14418 (PA2A_GLOHA)
Protein
Acidic phospholipase A2
Gene
N/A
Organism
Gloydius halys (Chinese water mocassin) (Agkistrodon halys)
Status
Functioni
Snake venom phospholipase A2 (PLA2) that acts in vivo as an anti-thrombotic agent. Inhibits platelet aggregation induced by ADP, arachidonic acid, and thrombin. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
1 PublicationCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotationEC:3.1.1.4PROSITE-ProRule annotation
Cofactori
Ca2+2 PublicationsNote: Binds 1 Ca2+ ion.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 27 | Calcium; via carbonyl oxygenCombined sources2 Publications | 1 | |
Metal bindingi | 29 | Calcium; via carbonyl oxygenCombined sources2 Publications | 1 | |
Metal bindingi | 31 | Calcium; via carbonyl oxygenCombined sources2 Publications | 1 | |
Active sitei | 47 | By similarity | 1 | |
Metal bindingi | 48 | CalciumCombined sources2 Publications | 1 | |
Active sitei | 89 | By similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- lipid catabolic process Source: UniProtKB-KW
- phospholipid metabolic process Source: InterPro
Keywordsi
Molecular function | Blood coagulation cascade inhibiting toxin, Hemostasis impairing toxin, Hydrolase, Platelet aggregation inhibiting toxin, Toxin |
Biological process | Lipid degradation, Lipid metabolism |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.1.4, 195 |
Names & Taxonomyi
Protein namesi | Recommended name: Acidic phospholipase A2 (EC:3.1.1.4)Short name: svPLA2 Alternative name(s): APLA2 Phosphatidylcholine 2-acylhydrolase |
Organismi | Gloydius halys (Chinese water mocassin) (Agkistrodon halys) |
Taxonomic identifieri | 8714 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Crotalinae › Gloydius |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Pharmaceutical usei
The venom of this snake has been orally administered to patients in China for about 2000 years, mostly to treat thrombotic diseases.
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161597 | 1 – 124 | Acidic phospholipase A21 PublicationAdd BLAST | 124 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 26 ↔ 116 | Combined sources2 Publications | ||
Disulfide bondi | 28 ↔ 44 | Combined sources2 Publications | ||
Disulfide bondi | 43 ↔ 95 | Combined sources2 Publications | ||
Disulfide bondi | 49 ↔ 124 | Combined sources2 Publications | ||
Disulfide bondi | 50 ↔ 88 | Combined sources2 Publications | ||
Disulfide bondi | 57 ↔ 81 | Combined sources2 Publications | ||
Disulfide bondi | 75 ↔ 86 | Combined sources2 Publications |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.1 Publication
Interactioni
Subunit structurei
Monomer.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14418 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14418 |
Family & Domainsi
Sequence similaritiesi
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
P14418-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
SLIQFETLIM KVAKKSGMFW YSNYGCYCGW GGQGRPQDAT DRCCFVHDCC
60 70 80 90 100
YGKVTGCDPK MDVYSFSEEN GDIVCGGDDP CKKEICECDR AAAICFRDNL
110 120
TLYNDKKYWA FGAKNCPQEE SEPC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 101 – 102 | TL → NT AA sequence (PubMed:18456297).Curated | 2 |
Mass spectrometryi
Molecular mass is 13962 Da. Determined by ESI. 1 Publication
Sequence databases
PIRi | A26535 |
Similar proteinsi
Cross-referencesi
Sequence databases
PIRi | A26535 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BK9 | X-ray | 2.00 | A | 1-124 | [»] | |
1PSJ | X-ray | 2.00 | A | 1-124 | [»] | |
SMRi | P14418 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
BRENDAi | 3.1.1.4, 195 |
Miscellaneous databases
EvolutionaryTracei | P14418 |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PA2A_GLOHA | |
Accessioni | P14418Primary (citable) accession number: P14418 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, PharmaceuticalDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families