UniProtKB - P14407 (FUMB_ECOLI)
Protein
Fumarate hydratase class I, anaerobic
Gene
fumB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reversible hydration of fumarate to (S)-malate. Functions in the generation of fumarate for use as an anaerobic electron acceptor. To a lesser extent, also displays D-tartrate dehydratase activity, but is not able to convert (R)-malate, L-tartrate or meso-tartrate. Is required for anaerobic growth on D-tartrate.4 Publications
Catalytic activityi
Cofactori
[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication
Kineticsi
- KM=300 µM for (S)-malate1 Publication
- KM=320 µM for fumarate1 Publication
- KM=800 µM for D-tartrate1 Publication
- Vmax=490 µmol/min/mg enzyme for (S)-malate dehydration1 Publication
- Vmax=1430 µmol/min/mg enzyme for fumarate hydration1 Publication
- Vmax=9.2 µmol/min/mg enzyme for D-tartrate dehydration1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 105 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 224 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 318 | Iron-sulfur (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- D(-)-tartrate dehydratase activity Source: EcoCyc
- fumarate hydratase activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- tricarboxylic acid cycle Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:FUMB-MONOMER MetaCyc:FUMB-MONOMER |
SABIO-RKi | P14407 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fumB1 Publication Ordered Locus Names:b4122, JW4083 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Disruption of this gene seriously impairs growth under anaerobic conditions on D-tartrate when glycerol is supplied as an electron donor (D-tartrate fermentation). Cells lacking this gene also lose most (about 79%) of D-tartrate dehydratase activity.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195660 | 1 – 548 | Fumarate hydratase class I, anaerobicAdd BLAST | 548 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 192 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P14407 |
PaxDbi | P14407 |
PRIDEi | P14407 |
PTM databases
iPTMneti | P14407 |
Expressioni
Inductioni
Is mainly expressed during anaerobic growth. Is under the control of the Fnr transcriptional regulator.1 Publication
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4262150, 11 interactors |
IntActi | P14407, 6 interactors |
STRINGi | 511145.b4122 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I fumarase family.Curated
Phylogenomic databases
eggNOGi | COG1838, Bacteria COG1951, Bacteria |
HOGENOMi | CLU_026758_0_0_6 |
InParanoidi | P14407 |
PhylomeDBi | P14407 |
Family and domain databases
Gene3Di | 3.20.130.10, 1 hit |
InterProi | View protein in InterPro IPR004646, Fe-S_hydro-lyase_TtdA-typ_cat IPR004647, Fe-S_hydro-lyase_TtdB-typ_cat IPR036660, Fe-S_hydroAse_TtdB_cat_sf IPR011167, Fe_dep_fumarate_hydratase IPR020557, Fumarate_lyase_CS |
Pfami | View protein in Pfam PF05681, Fumerase, 1 hit PF05683, Fumerase_C, 1 hit |
PIRSFi | PIRSF001394, Fe_dep_fumar_hy, 1 hit |
SUPFAMi | SSF117457, SSF117457, 1 hit |
TIGRFAMsi | TIGR00722, ttdA_fumA_fumB, 1 hit TIGR00723, ttdB_fumA_fumB, 1 hit |
PROSITEi | View protein in PROSITE PS00163, FUMARATE_LYASES, 1 hit |
i Sequence
Sequence statusi: Complete.
P14407-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNKPFIYQA PFPMGKDNTE YYLLTSDYVS VADFDGETIL KVEPEALTLL
60 70 80 90 100
AQQAFHDASF MLRPAHQKQV AAILHDPEAS ENDKYVALQF LRNSEIAAKG
110 120 130 140 150
VLPTCQDTGT AIIVGKKGQR VWTGGGDEET LSKGVYNTYI EDNLRYSQNA
160 170 180 190 200
ALDMYKEVNT GTNLPAQIDL YAVDGDEYKF LCVAKGGGSA NKTYLYQETK
210 220 230 240 250
ALLTPGKLKN FLVEKMRTLG TAACPPYHIA FVIGGTSAET NLKTVKLASA
260 270 280 290 300
HYYDELPTEG NEHGQAFRDV QLEQELLEEA QKLGLGAQFG GKYFAHDIRV
310 320 330 340 350
IRLPRHGASC PVGMGVSCSA DRNIKAKINR EGIWIEKLEH NPGQYIPQEL
360 370 380 390 400
RQAGEGEAVK VDLNRPMKEI LAQLSQYPVS TRLSLTGTII VGRDIAHAKL
410 420 430 440 450
KELIDAGKEL PQYIKDHPIY YAGPAKTPAG YPSGSLGPTT AGRMDSYVDL
460 470 480 490 500
LQSHGGSMIM LAKGNRSQQV TDACHKHGGF YLGSIGGPAA VLAQQSIKHL
510 520 530 540
ECVAYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIVN KQCANCTK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 50 | L → V in AAA23827 (PubMed:2656658).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27058 Genomic DNA Translation: AAA23827.1 U14003 Genomic DNA Translation: AAA97022.1 U00096 Genomic DNA Translation: AAC77083.1 AP009048 Genomic DNA Translation: BAE78124.1 |
PIRi | A65222, B44511 |
RefSeqi | NP_418546.1, NC_000913.3 WP_000066707.1, NZ_SSZK01000018.1 |
Genome annotation databases
EnsemblBacteriai | AAC77083; AAC77083; b4122 BAE78124; BAE78124; BAE78124 |
GeneIDi | 948642 |
KEGGi | ecj:JW4083 eco:b4122 |
PATRICi | fig|1411691.4.peg.2578 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27058 Genomic DNA Translation: AAA23827.1 U14003 Genomic DNA Translation: AAA97022.1 U00096 Genomic DNA Translation: AAC77083.1 AP009048 Genomic DNA Translation: BAE78124.1 |
PIRi | A65222, B44511 |
RefSeqi | NP_418546.1, NC_000913.3 WP_000066707.1, NZ_SSZK01000018.1 |
3D structure databases
SMRi | P14407 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262150, 11 interactors |
IntActi | P14407, 6 interactors |
STRINGi | 511145.b4122 |
PTM databases
iPTMneti | P14407 |
Proteomic databases
jPOSTi | P14407 |
PaxDbi | P14407 |
PRIDEi | P14407 |
Genome annotation databases
EnsemblBacteriai | AAC77083; AAC77083; b4122 BAE78124; BAE78124; BAE78124 |
GeneIDi | 948642 |
KEGGi | ecj:JW4083 eco:b4122 |
PATRICi | fig|1411691.4.peg.2578 |
Organism-specific databases
EchoBASEi | EB0352 |
Phylogenomic databases
eggNOGi | COG1838, Bacteria COG1951, Bacteria |
HOGENOMi | CLU_026758_0_0_6 |
InParanoidi | P14407 |
PhylomeDBi | P14407 |
Enzyme and pathway databases
BioCyci | EcoCyc:FUMB-MONOMER MetaCyc:FUMB-MONOMER |
SABIO-RKi | P14407 |
Miscellaneous databases
PROi | PR:P14407 |
Family and domain databases
Gene3Di | 3.20.130.10, 1 hit |
InterProi | View protein in InterPro IPR004646, Fe-S_hydro-lyase_TtdA-typ_cat IPR004647, Fe-S_hydro-lyase_TtdB-typ_cat IPR036660, Fe-S_hydroAse_TtdB_cat_sf IPR011167, Fe_dep_fumarate_hydratase IPR020557, Fumarate_lyase_CS |
Pfami | View protein in Pfam PF05681, Fumerase, 1 hit PF05683, Fumerase_C, 1 hit |
PIRSFi | PIRSF001394, Fe_dep_fumar_hy, 1 hit |
SUPFAMi | SSF117457, SSF117457, 1 hit |
TIGRFAMsi | TIGR00722, ttdA_fumA_fumB, 1 hit TIGR00723, ttdB_fumA_fumB, 1 hit |
PROSITEi | View protein in PROSITE PS00163, FUMARATE_LYASES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FUMB_ECOLI | |
Accessioni | P14407Primary (citable) accession number: P14407 Secondary accession number(s): P78139, Q2M6I2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | July 19, 2003 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families