UniProtKB - P14403 (POLG_JAEVN)
Genome polyprotein
Functioni
Catalytic activityi
- Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala. EC:3.4.21.91
Cofactori
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- double-stranded RNA binding Source: InterPro
- protein dimerization activity Source: InterPro
- RNA helicase activity Source: UniProtKB-EC
- serine-type endopeptidase activity Source: InterPro
- structural molecule activity Source: InterPro
GO - Biological processi
- clathrin-dependent endocytosis of virus by host cell Source: UniProtKB-KW
- fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
- suppression by virus of host STAT1 activity Source: UniProtKB-KW
- suppression by virus of host STAT2 activity Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Genome polyproteinCleaved into the following 9 chains: Alternative name(s): Core protein Alternative name(s): Matrix protein Alternative name(s): Flavivirin protease NS2B regulatory subunit Non-structural protein 2B Serine protease NS3 (EC:3.4.21.91By similarity, EC:3.6.1.15By similarity, EC:3.6.4.13By similarity) Alternative name(s): Flavivirin protease NS3 catalytic subunit Non-structural protein 3 |
Organismi | Japanese encephalitis virus (strain Nakayama) (JEV) |
Taxonomic identifieri | 11076 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Flasuviricetes › Amarillovirales › Flaviviridae › Flavivirus › |
Virus hosti | Ardeidae (herons) [TaxID: 8899] Bos taurus (Bovine) [TaxID: 9913] Culex gelidus [TaxID: 308713] Culex tritaeniorhynchus (Mosquito) [TaxID: 7178] Equus caballus (Horse) [TaxID: 9796] Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823] |
Subcellular locationi
- Virion By similarity
- Host nucleus By similarity
- Host cytoplasm By similarity
- host perinuclear region By similarity
- Secreted By similarity
- Virion membrane By similarity; Multi-pass membrane protein By similarity
- Host endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis Note: ER membrane retention is mediated by the transmembrane domains.By similarity
- Virion membrane Curated; Multi-pass membrane protein By similarity
- Host endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis Note: ER membrane retention is mediated by the transmembrane domains.By similarity
- Secreted By similarity
- Host endoplasmic reticulum membrane ; Peripheral membrane protein ; Lumenal side By similarity Note: Located in RE-derived vesicles hosting the replication complex.By similarity
- Host endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
- Host endoplasmic reticulum membrane ; Multi-pass membrane protein By similarity
- Host endoplasmic reticulum membrane PROSITE-ProRule annotation; Peripheral membrane protein PROSITE-ProRule annotation; Cytoplasmic side PROSITE-ProRule annotation Note: Remains non-covalently associated to serine protease subunit NS2B.PROSITE-ProRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 36 – 56 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 57 – 180 | ExtracellularSequence analysisAdd BLAST | 124 | |
Transmembranei | 181 – 201 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 202 – 207 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 208 – 222 | HelicalCuratedAdd BLAST | 15 | |
Topological domaini | 223 – 674 | ExtracellularSequence analysisAdd BLAST | 452 | |
Transmembranei | 675 – 695 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 696 – 701 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 702 – 722 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 723 – 1147 | ExtracellularSequence analysisAdd BLAST | 425 | |
Transmembranei | 1148 – 1168 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1169 – 1178 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 1179 – 1199 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1200 | LumenalSequence analysis | 1 | |
Transmembranei | 1201 – 1221 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1222 – 1237 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 1238 – 1258 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1259 – 1269 | LumenalSequence analysisAdd BLAST | 11 | |
Transmembranei | 1270 – 1290 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1291 – 1302 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 1303 – 1323 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1324 – 1326 | LumenalSequence analysis | 3 | |
Transmembranei | 1327 – 1347 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1348 – 1404 | CytoplasmicSequence analysisAdd BLAST | 57 | |
Intramembranei | 1405 – 1425 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1426 – ›1440 | CytoplasmicSequence analysisAdd BLAST | ›15 |
GO - Cellular componenti
- extracellular region Source: UniProtKB-SubCell
- host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
- host cell nucleus Source: UniProtKB-SubCell
- host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
- viral capsid Source: UniProtKB-KW
- viral envelope Source: UniProtKB-KW
- virion membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Capsid protein, Host cytoplasm, Host endoplasmic reticulum, Host membrane, Host nucleus, Membrane, Secreted, Viral envelope protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000405200 | ‹1 – ›1440 | Genome polyproteinAdd BLAST | ›1440 | |
ChainiPRO_0000037869 | ‹1 – 31 | Capsid protein CBy similarityAdd BLAST | ›31 | |
PropeptideiPRO_0000405201 | 32 – 53 | ER anchor for the capsid protein C, removed in mature form by serine protease NS3By similarityAdd BLAST | 22 | |
ChainiPRO_0000405202 | 54 – 222 | Protein prMBy similarityAdd BLAST | 169 | |
ChainiPRO_0000037870 | 54 – 146 | Peptide prBy similarityAdd BLAST | 93 | |
ChainiPRO_0000037871 | 147 – 222 | Small envelope protein MBy similarityAdd BLAST | 76 | |
ChainiPRO_0000037872 | 223 – 722 | Envelope protein EBy similarityAdd BLAST | 500 | |
ChainiPRO_0000037873 | 723 – 1074 | Non-structural protein 1By similarityAdd BLAST | 352 | |
ChainiPRO_0000037874 | 1075 – 1301 | Non-structural protein 2ABy similarityAdd BLAST | 227 | |
ChainiPRO_0000037875 | 1302 – 1432 | Serine protease subunit NS2BBy similarityAdd BLAST | 131 | |
ChainiPRO_0000037876 | 1433 – ›1440 | Serine protease NS3By similarity | ›8 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 68 | N-linked (GlcNAc...) asparagine; by hostBy similarity | 1 | |
Disulfide bondi | 225 ↔ 252 | By similarity | ||
Disulfide bondi | 282 ↔ 343 | By similarity | ||
Disulfide bondi | 282 ↔ 338 | By similarity | ||
Disulfide bondi | 296 ↔ 327 | By similarity | ||
Disulfide bondi | 314 ↔ 343 | By similarity | ||
Disulfide bondi | 314 ↔ 338 | By similarity | ||
Glycosylationi | 376 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Disulfide bondi | 412 ↔ 509 | By similarity | ||
Disulfide bondi | 526 ↔ 557 | By similarity | ||
Disulfide bondi | 726 ↔ 737 | By similarity | ||
Disulfide bondi | 777 ↔ 865 | By similarity | ||
Glycosylationi | 852 | N-linked (GlcNAc...) asparagine; by hostBy similarity | 1 | |
Disulfide bondi | 901 ↔ 945 | By similarity | ||
Glycosylationi | 929 | N-linked (GlcNAc...) asparagine; by hostBy similarity | 1 | |
Disulfide bondi | 1002 ↔ 1051 | By similarity | ||
Disulfide bondi | 1013 ↔ 1034 | By similarity | ||
Disulfide bondi | 1035 ↔ 1038 | By similarity |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 31 – 32 | Cleavage; by viral protease NS3By similarity | 2 | |
Sitei | 53 – 54 | Cleavage; by host signal peptidaseBy similarity | 2 | |
Sitei | 146 – 147 | Cleavage; by host furinBy similarity | 2 | |
Sitei | 222 – 223 | Cleavage; by host signal peptidaseBy similarity | 2 | |
Sitei | 722 – 723 | Cleavage; by host signal peptidaseBy similarity | 2 | |
Sitei | 1074 – 1075 | Cleavage; by hostBy similarity | 2 | |
Sitei | 1301 – 1302 | Cleavage; by viral protease NS3By similarity | 2 | |
Sitei | 1432 – 1433 | Cleavage; by autolysisBy similarity | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Homodimer (By similarity).
Interacts (via N-terminus) with host EXOC1 (via C-terminus); this interaction results in EXOC1 degradation through the proteasome degradation pathway (By similarity).
By similarityForms heterodimers with envelope protein E in the endoplasmic reticulum and Golgi.
By similarityHomodimer; in the endoplasmic reticulum and Golgi (By similarity).
Interacts with protein prM (By similarity).
Interacts with non-structural protein 1 (By similarity).
By similarityHomodimer; Homohexamer when secreted (By similarity).
Interacts with envelope protein E (By similarity). NS1 interacts with NS4B (By similarity).
Interacts with host complement protein CFH; this interaction leads to the degradation of C3 (By similarity).
By similarityForms a heterodimer with serine protease NS3 (By similarity). May form homooligomers (By similarity).
By similarityForms a heterodimer with NS2B (By similarity).
Interacts with non-structural protein 2A (via N-terminus) (By similarity).
Interacts with NS4B (By similarity).
Interacts with unphosphorylated RNA-directed RNA polymerase NS5; this interaction stimulates RNA-directed RNA polymerase NS5 guanylyltransferase activity (By similarity).
By similarityGO - Molecular functioni
- protein dimerization activity Source: InterPro
Structurei
Secondary structure
3D structure databases
SMRi | P14403 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 320 – 333 | Fusion peptideBy similarityAdd BLAST | 14 | |
Regioni | 1355 – 1394 | Interacts with and activates NS3 proteasePROSITE-ProRule annotationAdd BLAST | 40 |
Domaini
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd12149, Flavi_E_C, 1 hit |
Gene3Di | 1.10.8.970, 1 hit 1.20.1280.260, 1 hit 2.60.260.50, 1 hit 2.60.40.350, 1 hit 2.60.98.10, 1 hit 3.30.387.10, 1 hit 3.30.67.10, 1 hit |
InterProi | View protein in InterPro IPR000069, Env_glycoprot_M_flavivir IPR038302, Env_glycoprot_M_sf_flavivir IPR013755, Flav_gly_cen_dom_subdom1 IPR001122, Flavi_capsidC IPR027287, Flavi_E_Ig-like IPR026470, Flavi_E_Stem/Anchor_dom IPR038345, Flavi_E_Stem/Anchor_dom_sf IPR001157, Flavi_NS1 IPR000752, Flavi_NS2A IPR000487, Flavi_NS2B IPR002535, Flavi_propep IPR038688, Flavi_propep_sf IPR000336, Flavivir/Alphavir_Ig-like_sf IPR011998, Glycoprot_cen/dimer IPR036253, Glycoprot_cen/dimer_sf IPR038055, Glycoprot_E_dimer_dom IPR013756, GlyE_cen_dom_subdom2 IPR014756, Ig_E-set |
Pfami | View protein in Pfam PF01003, Flavi_capsid, 1 hit PF02832, Flavi_glycop_C, 1 hit PF00869, Flavi_glycoprot, 1 hit PF01004, Flavi_M, 1 hit PF00948, Flavi_NS1, 1 hit PF01005, Flavi_NS2A, 1 hit PF01002, Flavi_NS2B, 1 hit PF01570, Flavi_propep, 1 hit |
SUPFAMi | SSF56983, SSF56983, 1 hit SSF81296, SSF81296, 1 hit |
TIGRFAMsi | TIGR04240, flavi_E_stem, 1 hit |
PROSITEi | View protein in PROSITE PS51527, FLAVIVIRUS_NS2B, 1 hit |
i Sequence
Sequence statusi: Fragment.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
SVAMKHLTSF KRELGTLIDA VNKRGRKQNK RGGNEGSIMW LASLAVVIAC
60 70 80 90 100
AGAMKLSNFQ GKLLMTVNNT DIADVIVIPN PSKGENRCWV RAIDVGYMCE
110 120 130 140 150
DTITYECPKL TMGNDPEDVD CWCDNQEVYV QYGRCTRTRH SKRSRRSVSV
160 170 180 190 200
QTHGESSLVN KKEAWLDSTK ATRYLMKTEN WIVRNPGYAF LAAILGWMLG
210 220 230 240 250
SNNGQRRWYF TILLLLVAPA YSFNCLGMGN RDFIEGASGA TWVDLVLEGD
260 270 280 290 300
SCLTIMANDK PTLDVRMINI EAVQLAEVRS YCYHASVTDI STVARCPTTG
310 320 330 340 350
EAHNEKRADS SYVCKQGFTD RGWGNGCGLF GKGSIDTCAK FSCTSKAIGR
360 370 380 390 400
TIQPENIKYE VGIFVHGTTT SENHGNYSAQ VGASQAAKFT VTPNAPSITL
410 420 430 440 450
KLGDYGEVTL DCEPRSGLNT EAFYVMTVGS KSFLVHREWF HDLALPWTPP
460 470 480 490 500
SSTAWRNREL LMEFEEAHAT KQSVVALGSQ EGGLHQALAG AIVVEYSSSV
510 520 530 540 550
KLTSGHLKCR LKMDKLALKG TTYGMCTEKF SFAKNPADTG HGTVVIELSY
560 570 580 590 600
SGSDGPCKIP IVSVASLNDM TPVGRLVTVN PFVATSSANS KVLVEMEPPF
610 620 630 640 650
GDSYIVVGRG DKQINHHWHK AGSTLGKAFS TTLKGAQRLA ALGDTAWDFG
660 670 680 690 700
SIGGVFNSIG KAVHQVFGGA FRTLFGGMSW ITQGLMGALL LWMGVNARDR
710 720 730 740 750
SIALAFLATG GVLVFLATNV HADTGCAIDI TRKEMRCGSG IFVHNDVEAW
760 770 780 790 800
VDRYKYLPET PRSLAKIVHK AHKEGVCGVR SVTRLEHQMW EAVRDELNVL
810 820 830 840 850
LKENAVDLSV VVNKPVGRYR SAPKRLSMTQ EKFEMGWKAW GKSILFAPEL
860 870 880 890 900
ANSTFVVDGP ETKECPDEHR AWNSIEIEDF GFGITSTRVW LKIREESTDE
910 920 930 940 950
CDGAIIGTAV KGHVAVHSDL SYWIESRYND TWKLERAVFG EVKSCTWPET
960 970 980 990 1000
HTLWGDGVEE SELIIPHTIA GPKSKHNRRE GYKTQNQGPW DENGIVLDFD
1010 1020 1030 1040 1050
YCPGTKVTIT EDCGKRGPSV RTTTDSGKLI TDWCCRSCSL PPLRFRTENG
1060 1070 1080 1090 1100
CWYGMEIRPV RHDETTLVRS QVDAFNGEMV DPFQLGLLVM FLATQEVLRK
1110 1120 1130 1140 1150
RWTARLTIPA VLGALLVLML GGITYTDLAR YVVLVAAAFA EANSGGDVLH
1160 1170 1180 1190 1200
LALIAVFKIQ PAFLVMNMLS TRWTNQENVV LVLGAAFFHL ASVDLQIGVH
1210 1220 1230 1240 1250
GILNAAAIAW MIVRAITFPT TSSVTMPVLA LLTPGMRALY LDTYRIILLV
1260 1270 1280 1290 1300
IGICSLLQER KKTMAKKKGA VLLGLALTST GWFSPTTIAA GLMVCNPNKK
1310 1320 1330 1340 1350
RGWPATEFLS AVGLMFAIVG GLAELDIESM SIPFMLAGLM AVSYVVSGKA
1360 1370 1380 1390 1400
TDMWLERAAD ISWEMDAAIT GSSRRLDVKL DDDGDFHLID DPGVPWKVWV
1410 1420 1430 1440
LRMSCIGLAA LTPWAIVPAA FGYWLTLKTT KRGGVFWDTP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Non-terminal residuei | 1440 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16574 Genomic RNA Translation: AAA46251.1 |
PIRi | A27844, GNWVJF |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16574 Genomic RNA Translation: AAA46251.1 |
PIRi | A27844, GNWVJF |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4R8T | X-ray | 2.13 | A | 1352-1369 | [»] | |
SMRi | P14403 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protocols and materials databases
ABCDi | P14403, 1 sequenced antibody |
Family and domain databases
CDDi | cd12149, Flavi_E_C, 1 hit |
Gene3Di | 1.10.8.970, 1 hit 1.20.1280.260, 1 hit 2.60.260.50, 1 hit 2.60.40.350, 1 hit 2.60.98.10, 1 hit 3.30.387.10, 1 hit 3.30.67.10, 1 hit |
InterProi | View protein in InterPro IPR000069, Env_glycoprot_M_flavivir IPR038302, Env_glycoprot_M_sf_flavivir IPR013755, Flav_gly_cen_dom_subdom1 IPR001122, Flavi_capsidC IPR027287, Flavi_E_Ig-like IPR026470, Flavi_E_Stem/Anchor_dom IPR038345, Flavi_E_Stem/Anchor_dom_sf IPR001157, Flavi_NS1 IPR000752, Flavi_NS2A IPR000487, Flavi_NS2B IPR002535, Flavi_propep IPR038688, Flavi_propep_sf IPR000336, Flavivir/Alphavir_Ig-like_sf IPR011998, Glycoprot_cen/dimer IPR036253, Glycoprot_cen/dimer_sf IPR038055, Glycoprot_E_dimer_dom IPR013756, GlyE_cen_dom_subdom2 IPR014756, Ig_E-set |
Pfami | View protein in Pfam PF01003, Flavi_capsid, 1 hit PF02832, Flavi_glycop_C, 1 hit PF00869, Flavi_glycoprot, 1 hit PF01004, Flavi_M, 1 hit PF00948, Flavi_NS1, 1 hit PF01005, Flavi_NS2A, 1 hit PF01002, Flavi_NS2B, 1 hit PF01570, Flavi_propep, 1 hit |
SUPFAMi | SSF56983, SSF56983, 1 hit SSF81296, SSF81296, 1 hit |
TIGRFAMsi | TIGR04240, flavi_E_stem, 1 hit |
PROSITEi | View protein in PROSITE PS51527, FLAVIVIRUS_NS2B, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | POLG_JAEVN | |
Accessioni | P14403Primary (citable) accession number: P14403 Secondary accession number(s): P08769 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references